CRISPR Plasmids: gRNAs
The table below lists gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that you determine if your cell type contains the target sequence before using any of these gRNA plasmids and review the publication associated with each plasmid for more information on how it was originally used.
Here are a few questions to consider when choosing a gRNA:
- Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce the gRNA activity. The variety in sequence decreases the gRNA activity and increases off-target effect.
- Which species or variant of Cas9 (S. pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible with a single species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are upstream of a 5' NGG 3' PAM sequence.
- Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments use targets within promoters.
Do you have gRNAs you'd like to add to the Addgene collection? Click here to start the deposit process and have your plasmids added to the list. You can also email [email protected] for more information and assistance with the deposit process.
Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, PAM requirements, experimental considerations, and more, created and maintained by the Doudna Lab.
Please note that the following table lists both published and unpublished plasmids containing gRNA sequences designed to be used with any CRISPR nuclease and function. Please see the publication or plasmid information page, or contact the depositing PI for more information on the experimental use of these plasmids.