CRISPR/Cas9 Plasmids and Resources
Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use the CRISPR/Cas9 technology in your own lab. Browse plasmids below or check out our CRISPR resources on how to start using CRISPR in your lab.
Addgene CRISPR Resources
- CRISPR Guide: Essential background information on CRISPR/Cas9 and the basics for planning your first CRISPR experiment.
- CRISPR 101 eBook: Based on our popular "CRISPR 101" blog series, we've organized a comprehensive CRISPR resource for you to download.
- CRISPR Resources: Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and more.
- CRISPR Pooled Libraries: Find more information about using gRNA pooled libraries and browse our current list of all pooled libraries.
- CRISPR History: Learn how CRISPR/Cas9 was modified from a bacterial immune system to a revolutionary tool for genome engineering.
- Validated gRNA Sequences: Search or deposit gRNA sequences that have been described in peer reviewed publications.
- Blog Posts: Want the latest news on CRISPR? Experts cover CRISPR/Cas9 topics on Addgene's blog.
- Addgene's Viral Service: Request ready-to-use viral preps of select CRISPR lentiviral and AAV plasmids.
Browse CRISPR Plasmids by Function
|Cut||Wild type Cas9 efficiently generates double strand breaks (DSBs) at sequences homologous to co-expressed gRNA.|
|Nick||A mutated "nickase" version of the Cas9 enzyme generates a single-strand DNA break (Nick), instead of a double-strand DNA break (Cut).|
|Interfere||A catalytically inactive Cas9 (dCas9) can knockdown gene expression by interfering with transcription. The dCas9 can sometimes be fused to an additional repressor peptide.|
|Activate||A catalytically inactive Cas9 (dCas9) fused to an activator peptide can activate or increase gene expression.|
|dCas9-FokI||A catalytically inactive Cas9 (dCas9) fused to FokI nuclease to generate double strand breaks (DSBs, Cut) at sequences homologous to two co-expressed gRNA.|
|Purify||Isolate specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused with an epitope tag(s).|
|Visualize||Visualize specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused to a fluorescent protein.|
|Screen||Use pooled CRISPR libraries to screen for genes involved in specific biological processes.|
|Tag||Find the tools for tagging your endogenous protein of interest.|
|Validated gRNAs||Experimentally validated, pre-made gRNA plasmids to specific genes.|
|Empty gRNA Vectors||Select a gRNA plasmid based on a variety of factors, such as presence or absence of Cas9 and number of gRNAs (multiplex vectors).|
Browse Plasmids by Model Organism