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CRISPR main page header CRISPR/Cas9 Plasmids and Resources

Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use the CRISPR/Cas9 technology in your own lab. Browse plasmids below or check out our CRISPR resources on how to start using CRISPR in your lab.

Addgene CRISPR Resources

  • CRISPR Guide: Essential background information on CRISPR/Cas9 and the basics for planning your first CRISPR experiment.
  • CRISPR 101 eBook: Based on our popular "CRISPR 101" blog series, we've organized a comprehensive CRISPR resource for you to download.
  • CRISPR Resources: Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to CRISPR forums, and more.
  • CRISPR Pooled Libraries: Find more information about using gRNA pooled libraries and browse our current list of all pooled libraries.
  • CRISPR History: Learn how CRISPR/Cas9 was modified from a bacterial immune system to a revolutionary tool for genome engineering.
  • Validated gRNA Sequences: Search or deposit gRNA sequences that have been described in peer reviewed publications.
  • Blog Posts: Want the latest news on CRISPR? Experts cover CRISPR/Cas9 topics on Addgene's blog.
  • Addgene's Viral Service: Request ready-to-use viral preps of select CRISPR lentiviral and AAV plasmids.

Browse CRISPR Plasmids by Function

CRISPR cut cartoon button Cut Wild type Cas9 efficiently generates double strand breaks (DSBs) at sequences homologous to co-expressed gRNA.
CRISPR nick cartoon button Nick A mutated "nickase" version of the Cas9 enzyme generates a single-strand DNA break (Nick), instead of a double-strand DNA break (Cut).
CRISPR interfere cartoon button Interfere A catalytically inactive Cas9 (dCas9) can knockdown gene expression by interfering with transcription. The dCas9 can sometimes be fused to an additional repressor peptide.
CRISPR activate cartoon button Activate A catalytically inactive Cas9 (dCas9) fused to an activator peptide can activate or increase gene expression.
CRISPR cut cartoon button dCas9-FokI A catalytically inactive Cas9 (dCas9) fused to FokI nuclease to generate double strand breaks (DSBs, Cut) at sequences homologous to two co-expressed gRNA.
CRISPR purify cartoon button Purify Isolate specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused with an epitope tag(s).
CRISPR visualize cartoon button Visualize Visualize specific genomic regions of interest using a catalytically inactive Cas9 (dCas9) fused to a fluorescent protein.
CRISPR libraries cartoon button Screen Use pooled CRISPR libraries to screen for genes involved in specific biological processes.
CRISPR flag tag icon Tag Find the tools for tagging your endogenous protein of interest.
empty gRNA cartoon button Validated gRNAs Experimentally validated, pre-made gRNA plasmids to specific genes.
Round_Empty_gRNA_Expression_Icon.png Empty gRNA Vectors Select a gRNA plasmid based on a variety of factors, such as presence or absence of Cas9 and number of gRNAs (multiplex vectors).

Browse Plasmids by Model Organism

Mammalian Bacteria Drosophila Plant
C. elegans Yeast Zebrafish Xenopus
Click to Download CRISPR 101 2nd Edition-01.png