| MLM3613 | Streptococcus pyogenes Cas9 | CRISPR ; zebrafish expression | CMV |
Joung
| Efficient genome editing in zebrafish using a CRISPR-Cas system.
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
|
Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters
unknown
|
| MLM3639 | Streptococcus pyogenes Cas9-3X Flag | CRISPR ; zebrafish expression | CMV |
Joung
| Efficient genome editing in zebrafish using a CRISPR-Cas system.
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
|
unknown
|
| pST1374-NLS-flag-linker-Cas9 | Human codon optimized Cas9 | Mammalian Expression, CRISPR | CMV |
Huang
| Generation of gene-modified mice via Cas9/RNA-mediated gene targeting.
Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46.
|
pST1374 (Addgene #13426)
|
| pT3TS-nCas9n | Cas9 (Synthetic) | Bacterial Expression, CRISPR | T3 |
Chen
| Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system.
Proc Natl Acad Sci U S A. 2013 Aug 5.
|
pT3T7
|
| pCS2-Cas9 | Cas9 (Other) | Mammalian Expression, CRISPR ; xenopus and zebrafish expression | SP6 |
Schier
| Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014.
|
For in vivo expression of Cas9 or generation of Cas9 mRNA
pCS2
|
| pCS2-nCas9n | nls-zcas9-nls (Synthetic) | Mammalian Expression, CRISPR | CMV |
Chen
| Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system.
Proc Natl Acad Sci U S A. 2013 Aug 5.
|
expression of an optimized Cas9 for genome-editing in zebrafish
pCS2+
|
| pCS2+hSpCas9 | humanized S.pyogenes Cas9 | Mammalian Expression, CRISPR ; medaka, zebrafish | SP6 |
Kinoshita
| Targeted mutagenesis using CRISPR/Cas system in medaka.
Biol Open. 2014 Apr 11;3(5):362-71. doi: 10.1242/bio.20148177.
|
Encode hSpCas9 for in vitro transcription with Sp6
pCS2
|
| pCS2-nCas9n-nanos 3’UTR | nanos 3' UTR (Danio rerio) | CRISPR | SP6 |
Giraldez
| CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo.
Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3543.
|
expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish
pCS2-nCas9n
nanos3 cb725, nanos, nanos1, nos1
|
| pME-Cas9 | Cas9 (Danio rerio) | CRISPR ; Tol2 Middle Entry vector for Gateway | |
Zon
| A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish.
Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032.
|
Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS
pME-MCS (Tol2Kit #237)
|
| pME-Cas9-T2A-GFP | Cas9-T2A-GFP (Danio rerio) | CRISPR ; Tol2 Middle Entry vector for Gateway | |
Zon
| A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish.
Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032.
|
Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
pME-MCS (Tol2Kit #237)
|
| pME-Cas9 | codon optimized Cas9 (Other) | CRISPR | |
Chen
| Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.
Genetics. 2015 Apr 8. pii: genetics.115.176917.
|
expresses codon optimized Cas9 in zebrafish
pME-MCS
|
| pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 | 5x UAS (Saccharomyces cerevisiae), Cas9 (Other), T2A, GFP, U6 promoter (Danio rerio) | CRISPR | |
Del Bene
| 2C-Cas9: a versatile tool for clonal analysis of gene function.
Genome Res. 2016 Mar 8.
|
Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter
pminiTol2
|
| pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2 | 5xUAS, Cas9, Cre, U6 promoter | CRISPR | |
Del Bene
| 2C-Cas9: a versatile tool for clonal analysis of gene function.
Genome Res. 2016 Mar 8.
|
Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter
pminiTol2
|
| pMJ922 | SpCas9 (Other) | Bacterial Expression | T7 |
Jinek
| Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes.
Development. 2016 Apr 29. pii: dev.134809.
|
Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
pET His6 MBP N10 TEV LIC cloning vector (2C-T)
|
| pMJ923 | SpCas9 (Other) | Bacterial Expression | T7 |
Jinek
| Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes.
Development. 2016 Apr 29. pii: dev.134809.
|
Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
pET His6 MBP N10 TEV LIC cloning vector (2C-T)
|
| pT3TS-zAsCpf1 | AsCpf1 (Danio rerio) | CRISPR | |
Giraldez
| CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing.
Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
|
plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)
pT3TS
|
| pME-cas9-2A-mCherry | cas9-2A-mCherry (Danio rerio) | CRISPR | |
Deng
| Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish.
Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 10.1242/dmm.033027.
|
An entry vector with cas9 -2A-mCherry
pME
|
| pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA | 5xU6:sgRNA (Danio rerio), Cas9-t2A-GFP (Danio rerio) | CRISPR | U6, hsp70 |
Schier
| Simultaneous single-cell profiling of lineages and cell types in the vertebrate brain.
Nat Biotechnol. 2018 Mar 28. pii: nbt.4103. doi: 10.1038/nbt.4103.
|
Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish
Tol2
|
| pME-nCas9n-P2A-mKate2-pA | nCas9n and mKate2 (Synthetic) | Zebrafish plasmids | |
Parton
|
Parton Lab Plasmids for in vivo cell biology in the zebrafish
(unpublished)
|
Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2
pDONR 221
|
| MiniCoopR U6:gRNA, mitfa:Cas9 | | CRISPR ; Tol2 | |
Zon
| Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma.
Science. 2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509.
|
Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes
MiniCoopR
|
| MiniCoopR 2xU6:gRNA, mitfa:Cas9 | | CRISPR ; Tol2 | |
Zon
| Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma.
Science. 2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509.
|
Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes
MiniCoopR
|
| pT3TS-nCas9n-A71 | nCas9n (Other) | RNA in vitro transcription vector | T3 |
Berman
|
Genome editing in zebrafish using high-fidelity Cas9 nucleases
(unpublished)
|
micro-injection of poly-adenylated nCas9n RNA
pT3TS
|
| pT3TS-Hypa-nCas9n-A69 | Hypa-nCas9n (Other) | RNA in vitro transcription vector | T3 |
Berman
|
Genome editing in zebrafish using high-fidelity Cas9 nucleases
(unpublished)
|
micro-injection of poly-adenylated Hypa-nCas9n RNA
pT3TS
|
| pT3TS-HiFi-nCas9n-A72 | HiFi-nCas9n (Other) | RNA in vitro transcription vector | T3 |
Berman
|
Genome editing in zebrafish using high-fidelity Cas9 nucleases
(unpublished)
|
micro-injection of poly-adenylated HiFi-nCas9n RNA
pT3TS
|
| pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP | hsp70l promoter (Danio rerio), floxed DsRed cassette (Synthetic), Cas9-GFP (Synthetic) | Cre/Lox, CRISPR | |
Brand
| Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish.
Nature Communications. 2021. 12: 1125.
|
Cre-dependet Cas9-GFP expression
pBluescript
|
| pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP | hsp70l promoter (Danio rerio), floxed Non-FP cassette (Synthetic), Cas9-GFP (Synthetic) | Cre/Lox, CRISPR | |
Brand
| Cell-fate plasticity, adhesion and cell sorting complementarily establish a sharp midbrain-hindbrain boundary.
Development. 2020 Jun 8;147(11). pii: dev.186882. doi: 10.1242/dev.186882.
|
Cre-dependet Cas9-GFP expression
pBluescript
|