| p414-TEF1p-Cas9-CYC1t | Human Optimized S. pyogenes Cas9 (Homo sapiens) | TEF1 promoter | TRP1 |
Church
| Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.
Nucleic Acids Res
|
p414
|
| p415-GalL-Cas9-CYC1t | Human Optimized S. pyogenes Cas9 (Homo sapiens) | GalL | LEU2 |
Church
| Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.
Nucleic Acids Res
|
p415
|
| pACT2-CAS9c | Cas9c (Synthetic) | yeast ADH1 promoter | LEU2 |
Zhao
| Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing.
J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
|
Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
pACT2
|
| pMZ222 | Cas9 (Other) | adh1 | LEU2 |
Zaratiegui
| Implementation of the CRISPR-Cas9 system in fission yeast.
Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
|
Constitutive expression of humanized Cas9
pART1
|
| pMZ374 | Cas9 (Other), rrk1:sgRNA (Other) | adh1, rrk1 | ura4 |
Zaratiegui
| Implementation of the CRISPR-Cas9 system in fission yeast.
Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
|
Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).
pMZ283
|
| pCT | iCas9 (Other), tracrRNA (Other) | TEF1p, RPR1p | LEU2 |
Zhao
| Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae.
ACS Synth Biol. 2014 Sep 19.
|
Plasmid encoding iCas9 and tracrRNA on pRS415 backbone
pRS415
|
| pCRCT | iCas9 (Other), tracrRNA (Other) | TEF1p, RPR1p | URA3 |
Zhao
| Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae.
ACS Synth Biol. 2014 Sep 19.
|
Plasmid encoding iCas9, tracrRNA and crRNAs
pRS426
|
| Cas9-NAT | Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette | | Nourseothricin(clonNat) |
Jin
| Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.
Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3.
|
Cas9 expression cassette carried by a yeast single-copy episome plasmid
pRS414-TEF1p-Cas9-CYC1t
|
| pML104 | Cas9 (Saccharomyces cerevisiae), single guide RNA expression cassette (Saccharomyces cerevisiae) | pTDH3, pSNR52 | URA3 |
Wyrick
| New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
pRSII426
|
| pML107 | Cas9 (Saccharomyces cerevisiae), single guide RNA expression cassette (Saccharomyces cerevisiae) | pTDH3 (aka GAP promoter), pSNR52 | LEU2 |
Wyrick
| New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
pRSII425
|
| pJH001 | Cas9 (Saccharomyces cerevisiae), Empty | | LEU2 |
Wyrick
| New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.
Yeast. 2015 Dec;32(12):711-20. doi: 10.1002/yea.3098. Epub 2015 Sep 21.
|
High copy Cas9 expression vector with LEU2 marker for yeast transformation
pRSII425
|
| p415-PtrpC-Cas9-TtrpC-CYC1t | humanized Cas9 (Homo sapiens) | | LEU2 |
Hong
| Efficient gene editing in Neurospora crassa with CRISPR technology
Fungal Biology and Biotechnology 2015, 2:4
|
Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.
pRS415
|
| pCRISPRyl | Codon optimized Cas9 from S. pyogenes (Synthetic), sgRNA expression cassette (Synthetic) | UAS1B8-TEF(136), SCR1'-tRNA | LEU2 |
Wheeldon
| Synthetic RNA polymerase III promoters facilitate high efficiency CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica.
ACS Synth Biol. 2015 Dec 29.
|
CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
pUC19
|
| pMZ376 | Cas9 (Other), rrk1:sgRNA (Other) | adh1, rrk1 | Neomycin (select with G418) |
Zaratiegui
|
sgRNA/Cas9
(unpublished)
|
Combined sgRNA/Cas9 vector with KanMX marker
pMZ374
|
| pMZ377 | Cas9 (Other), rrk1:sgRNA (Other) | adh1, rrk1 | LEU2 |
Zaratiegui
|
sgRNA/Cas9
(unpublished)
|
Combined sgRNA/Cas9 vector with LEU2 marker
pMZ374
|
| pMZ379 | Cas9 (Other), rrk1:sgRNA (Other) | adh1, rrk1 | nourseothricin |
Zaratiegui
|
sgRNA/Cas9
(unpublished)
|
Combined sgRNA/Cas9 vector with NatMX marker
pMZ374
|
| pCfB2312 (TEF1p-Cas9-CYC1t_kanMX) | Cas9 (Other) | | Gentamicin |
Borodina
| EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. Epub 2016 Jun 23.
|
Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance
pRS414
|
| pRS416gT-GalL-Cas9 | Cas9 (Synthetic), guide RNA (gRNA) (Synthetic), Tetracycline Repressor (Other) | GalL, RPR1 promoter with TetO site, GPM1 promoter | URA3 |
Davis
| Distinct patterns of Cas9 mismatch tolerance in vitro and in vivo.
Nucleic Acids Res. 2016 May 19. pii: gkw417.
|
pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
pRS416
|
| pRCC-K | Cas9 (Other), empty gRNA cassette (Synthetic) | ROX3, SNP52p | Neomycin (select with G418) |
Boles
| Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
|
Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
pRS42K
Tag
/ Fusion Protein- NTS (C terminal on backbone)
|
| pRCC-N | Cas9 (Other), empty gRNA cassette (Synthetic) | ROX3, SNP52p | Nourseothricin |
Boles
| Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
|
Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
pRS42N
Tag
/ Fusion Protein- NTS (C terminal on backbone)
|
| pDuRCC-K | Cas9 (Other), empty gRNA cassette (Synthetic), empty gRNA cassette (Synthetic) | ROX3, SNP52p, SNP52p | Neomycin (select with G418) |
Boles
| Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
|
Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
pRS42K
Tag
/ Fusion Protein- NTS (C terminal on backbone)
|
| pDuRCC-N | Cas9 (Other), empty gRNA cassette (Synthetic), empty gRNA cassette (Synthetic) | ROX3, SNP52p, SNP52p | Nourseothricin |
Boles
| Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae.
J Microbiol Methods. 2016 Aug;127:203-5. doi: 10.1016/j.mimet.2016.06.020. Epub 2016 Jun 17.
|
Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
pRS42N
Tag
/ Fusion Protein- NTS (C terminal on backbone)
|
| pML104-HygMx4 | HphMX4 (Other) | | Hygromycin |
Wittkopp
|
Patricia Wittkopp yeast CRISPR plasmids
(unpublished)
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
pRSII426
|
| pML104-KanMx4 | KanMX4 (Other) | | Neomycin (select with G418) |
Wittkopp
|
Patricia Wittkopp yeast CRISPR plasmids
(unpublished)
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
pRSII426
|
| pML104-NatMx3 | NatMx3 (Other) | | nourseothricin (nat) |
Wittkopp
|
Patricia Wittkopp yeast CRISPR plasmids
(unpublished)
|
Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
pRSII426
|
| pCfB2312 (TEF1p-Cas9-CYC1t_kanMX) | Cas9 (Other) | | G418 |
Borodina
| CRISPR–Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains
Metab Eng Commun 2015
|
Cas9-carrying vector with kanMX marker
p414-TEF1p-Cas9-CYC1t
|
| pCRISPRyl_AXP | Cas9 | UAS1B8-TEF(136) | LEU2 |
Wheeldon
| Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at AXP locus in Y. lipolytica
pUC19
|
| pCRISPRyl_XPR2 | Cas9 | UAS1B8-TEF(136) | LEU2 |
Wheeldon
| Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at XPR2 locus in Y. lipolytica
pUC19
|
| pCRISPRyl_A08 | Cas9 | UAS1B8-TEF(136) | LEU2 |
Wheeldon
| Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at A08 locus in Y. lipolytica
pUC19
|
| pCRISPRyl_D17 | Cas9 | UAS1B8-TEF(136) | LEU2 |
Wheeldon
| Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at D17 locus in Y. lipolytica
pUC19
|
| pCRISPRyl_MFE1 | Cas9 | UAS1B8-TEF(136) | LEU2 |
Wheeldon
| Standardized markerless gene integration for pathway engineering in Yarrowia lipolytica.
ACS Synth Biol. 2016 Dec 19.
|
Introduces DSB at MFE1 locus in Y. lipolytica
pUC19
|
| pJK-caCas9-NatMX-Neut5L | | | Nourseothricin |
Church
| A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans.
Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23.
|
caCas9 integrating vector into the Neut5L locus
pJK1027
|
| pJK-caCas9-NatMX-Neut5L-Ade2 drive | Ade2 gene drive (Other) | | Nourseothricin |
Church
| A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans.
Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23.
|
caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus
pJK1027
|
| pJK-caCas9-NatMX-Neut5L-Leu2 drive | Leu2 gene drive (Other) | | |
Church
| A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans.
Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23.
|
caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus
pjk1027
|
| pWS158 | Cas9 (Saccharomyces cerevisiae) | | URA3 |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - URA3
2 micron
|
| pWS171 | Cas9 (Saccharomyces cerevisiae) | | LEU2 |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - LEU2
2 micron
|
| pWS172 | Cas9 (Saccharomyces cerevisiae) | | HIS3 |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - HIS3
2 micron
|
| pWS173 | Cas9 (Saccharomyces cerevisiae) | | Kanamycin (select with G418) |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - KanR
2 micron
|
| pWS174 | Cas9 (Saccharomyces cerevisiae) | | Nourseothricin |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - NatR
2 micron
|
| pWS175 | Cas9 (Saccharomyces cerevisiae) | | Hygromycin |
Ellis
|
Ellis Lab Yeast CRISPR Plasmids
(unpublished)
|
Cas9 gap repair vector - HygR
2 micron
|
| pADH99 | US_HIS1, FRT, pENO1, C. albicans Cas9, pMAL2, FLP, NAT 1 of 2 | | Nourseothricin (NAT) |
Hernday
| An efficient, rapid, and recyclable system for CRISPR-mediated genome editing in Candida albicans
mSphere Apr 2017, 2 (2) e00149-17
|
NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
pUC19
|
| pADH137 | C. albicans LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2 | | Nourseothricin (NAT) |
Hernday
| An efficient, rapid, and recyclable system for CRISPR-mediated genome editing in Candida albicans
mSphere Apr 2017, 2 (2) e00149-17
|
NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.
pUC19
|
| pADH140 | C. maltosa LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2 | | Nourseothricin (NAT) |
Hernday
| An efficient, rapid, and recyclable system for CRISPR-mediated genome editing in Candida albicans
mSphere Apr 2017, 2 (2) e00149-17
|
NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.
pUC19
|
| pDHt/sk-PC | Cas9 (Other) | Ppdc | Hygromycin |
Zhou
| Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.
Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015.
|
Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei
pDHt/sk
|
| pDHt/sk-PE | Cas9-eGFP (Other) | Ppdc | Hygromycin |
Zhou
| Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.
Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015.
|
Expression of codon-optimized Cas9-eGFP fusion protein with Ppdc promoter in Trichoderma reesei
pDHt/sk
Tag
/ Fusion Protein- EGFP (C terminal on insert)
|
| pDHt/sk-CC | Cas9 (Other) | Pcbh1 | Hygromycin |
Zhou
| Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.
Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015.
|
Expression of codon-optimized Cas9 with Pcbh1 promoter in Trichoderma reesei
pDHt/sk
|
| pDHt/sk-CE | Cas9-eGFP (Other) | Pcbh2 | Hygromycin |
Zhou
| Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system.
Cell Discov. 2015 May 12;1:15007. doi: 10.1038/celldisc.2015.7. eCollection 2015.
|
Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei
pDHt/sk
Tag
/ Fusion Protein- EGFP (C terminal on insert)
|
| pYZ033 | | | Spura4 |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
Entry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1
pMZ374
|
| pYZ146 | gRNA targeting Sp.leu1 (Schizosaccharomyces pombe) | | Spura4 |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe
pYZ033
leu1 SPBC1A4.02c
|
| pYZ164 | gRNA targeting Sp.his3 (Schizosaccharomyces pombe) | | Spura4 |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
Cas9-gRNA his3 plasmid for his3 deletion in S. pombe
pYZ033
his3 SPBC11B10.02c
|
| pYZ173 | gRNA targeting Sp.lys9 (Schizosaccharomyces pombe) | | Spura4 |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
Cas9-gRNA lys9 plasmid for lys9 deletion in S. pombe
pYZ033
SPBC3B8.03 SPBC3B8.03
|
| pDB4279 | Cas9 (Other) | | |
Du
| A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast.
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164.
|
A control plasmid containing the intact bsdMX marker
pMZ374 (Addgene 59896)
|
| pDB4280 | Cas9 (Other) | | |
Du
| A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast.
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164.
|
A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system
pMZ374 (Addgene 59896)
|
| pDB4281 | Cas9 (Other) | | |
Du
| A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast.
G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164.
|
A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system
pMZ374 (Addgene 59896)
|
| pIW447-CRISPR XYL2 | Codon optimized Cas9 (Synthetic), sgRNA expression cassette (Synthetic) | | URA3 |
Wheeldon
| CRISPR-Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus.
Biotechnol Biofuels. 2017 Jun 24;10:164. doi: 10.1186/s13068-017-0854-5. eCollection 2017.
|
K. marxianus CRISPR Plasmid for XYL2 editing
pJSK316-GPD
|
| pIW601-KmCRISPR | Codon optimized Cas9 (Synthetic), sgRNA expression cassette (Synthetic) | | URA3 |
Wheeldon
| CRISPR-Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus.
Biotechnol Biofuels. 2017 Jun 24;10:164. doi: 10.1186/s13068-017-0854-5. eCollection 2017.
|
K. marxianus CRISPR Plasmid for sgRNA cloning
pJSK316-GPD
|
| bRA89 | | | Hygromycin |
Haber
| Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.
Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12.
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker
pRS316
|
| bRA90 | | | LEU2 |
Haber
| Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.
Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12.
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker
pRS316
|
| bRA66 | | | Hygromycin |
Haber
| Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.
Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12.
|
GAL1 driven Cas9. gRNA can be cloned into BplI sites.
pRS316
|
| bRA77 | | | LEU2 |
Haber
| Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.
Nature. 2017 Apr 20;544(7650):377-380. doi: 10.1038/nature22046. Epub 2017 Apr 12.
|
GAL1 driven Cas9. gRNA can be cloned into BplI sites.
pRS316
|
| pJH2970 | | | HIS3 |
Haber
| Cas9-mediated gene editing in Saccharomyces cerevisiae
Protocol Exchange (2017)
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker
pRS316
|
| pJH2971 | | | Neomycin (select with G418) |
Haber
| Cas9-mediated gene editing in Saccharomyces cerevisiae
Protocol Exchange (2017)
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
pRS316
|
| pJH2972 | | | URA3 |
Haber
| Cas9-mediated gene editing in Saccharomyces cerevisiae
Protocol Exchange (2017)
|
Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker
pRS316
|
| pUDP004 | | ScTDH3 | amdS (acetamidase gene conferring growth on acetamide) |
Daran
| CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus.
Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1.
|
E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)
not applicable
|
| pUDP012 | HH-gRNA-HDV targetting SeILV6 in S. pastorianus (Saccharomyces cerevisiae) | ScTDH3, ScTDH3 | amdS (acetamidase gene conferring growth on acetamide) |
Daran
| CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus.
Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1.
|
E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)
pUDP004
|
| pUDP044 | polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus (Saccharomyces cerevisiae) | ScTDH3, ScTDH3 | amdS (acetamidase gene conferring growth on acetamide) |
Daran
| CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus.
Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1.
|
pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
pUDP004
|
| pCSN061 | S. pyogenes Cas9 codon optimized for expression in S. cerevisiae., KanMX marker expression cassette. (Other) | Heterologous promoter from K. lactis (KLLA0F20031g), Heterologous TEF1 promoter from A. gossypii. | TRP1 ; KanMX |
Verwaal
| CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae.
Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
|
Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
pRS414
NEWENTRY
|
| pCSN067 | Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae. (Other), KanMX marker expression cassette. (Other) | Heterologous promoter from K. lactis (KLLA0F20031g)., Heterologous TEF1 promoter from A. gossypii. | TRP1 ; KanMX |
Verwaal
| CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae.
Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
|
Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.
pRS414
|
| pCSN068 | Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae. (Other), KanMX marker expression cassette. (Other) | Heterologous promoter from K. lactis (KLLA0F20031g)., Heterologous TEF1 promoter from A. gossypii. | TRP1 ; KanMX |
Verwaal
| CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae.
Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
|
Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
pRS414
|
| pYZ292 | | | LEU2 ; Sc |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - ScLEU2 marker
pYZ292
|
| pYZ293 | | | Sp leu1 |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - Sp leu1 marker
pYZ293
|
| pYZ294 | | | kanMX |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - KanMX marker
pYZ294
|
| pYZ300 | | | Sp his3 |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - Sp his3 marker
pYZ300
|
| pYZ301 | | | Sp lys9 |
Boeke
| Construction of Designer Selectable Marker Deletions with CRISPR-Cas9 Toolbox in Schizosaccharomyces pombe and Optimized Design of Common Entry Vectors.
G3 (Bethesda). 2018 Jan 10. pii: g3.117.300363. doi: 10.1534/g3.117.300363.
|
S. pombe gRNA entry vector with Cas9 - Sp lys9 marker
pYZ301
|
| pUDE731 | Fncpf1 (Other) | TEF1 | URA3 |
Daran
| FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae.
Nucleic Acids Research (2017), gkx1007
|
S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p
pMEL10
|
| pUDC175 | Fncpf1 (Other) | TEF1 | TRP1 |
Daran
| FnCpf1, a novel and efficient genome editing tool for Saccharomyces cerevisiae.
Nucleic Acids Research (2017), gkx1007
|
S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter
p414TEF1
|
| pUDP002 | hph (Other), Spcas9 D147Y P411T (Other) | Ashbya gossypii (Eremothecium gossypii) TEF1, Arxula adeninivorans TEF1 | Hygromycin |
Daran
| Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid.
FEMS Yeast Res. 2018 May 1;18(3). pii: 4847887. doi: 10.1093/femsyr/foy012.
|
Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
not applicable
|
| BB3cN_pGAP_23*_pLAT1_Cas9 | HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) | | NTC |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
Episomal ARS/CEN Backbone
|
| BB3cH_pGAP_23*_pLAT1_Cas9 | HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) | | Hygromycin |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
Episomal ARS/CEN Backbone
|
| BB3cK_pGAP_23*_pLAT1_Cas9 | HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) | | Neomycin (select with G418) |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
Episomal ARS/CEN Backbone
|
| BB3cK_pGAP_23*_pTEF_Cas9 | HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) | | Neomycin (select with G418) |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
Episomal ARS/CEN Backbone
|
| BB3cK_pGAP_23*_pPFK300_Cas9 | HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) | | Neomycin (select with G418) |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
Episomal ARS/CEN Backbone
|
| BB3cH_pGAP_23*_pPFK300_Cas9 | HH - FS23 linker - HDV (Other), human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS) (Homo sapiens) | | Hygromycin |
Gasser
|
CRISPR/Cas9-mediated homology directed genome editing in Pichia pastoris
(unpublished)
|
hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
Episomal ARS/CEN Backbone
|
| pCfB4906 | Cas9 (Other) | | Hygromycin |
Borodina
| EasyCloneYALI: CRISPR/Cas9-Based Synthetic Toolbox for Engineering of the Yeast Yarrowia lipolytica.
Biotechnol J. 2018 Jan 29. doi: 10.1002/biot.201700543.
|
EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance
Unknown
|
| pCfB6364 | Cas9 (Other) | | D-serine marker; enables growth on D-serine |
Borodina
| EasyCloneYALI: CRISPR/Cas9-Based Synthetic Toolbox for Engineering of the Yeast Yarrowia lipolytica.
Biotechnol J. 2018 Jan 29. doi: 10.1002/biot.201700543.
|
EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance
Unknown
|
| pHCas9-Nours | Cas9 (Other) | TEF1 | URA3 |
Yang
| Unraveling the genetic basis of fast l-arabinose consumption on top of recombinant xylose-fermenting Saccharomyces cerevisiae.
Biotechnol Bioeng. 2018 Sep 10. doi: 10.1002/bit.26827.
|
Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae
pSH47
|
| pV1025 | CaCas9 (Synthetic) | | Ampicillin |
Fink
| A CRISPR system permits genetic engineering of essential genes and gene families.
Sci Adv. 2015;1(3):e1500248.
|
Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
pV941
|
| pV1093 | CaCas9/sgRNA-BsmBI stuffer (Synthetic) | | clonNAT |
Fink
| A CRISPR system permits genetic engineering of essential genes and gene families.
Sci Adv. 2015;1(3):e1500248.
|
CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
pV1088/pV1090
|
| pV1200 | CaCas9/sgRNA-BsmBI stuffer (Synthetic) | | Ampicillin |
Fink
| A CRISPR system permits genetic engineering of essential genes and gene families.
Sci Adv. 2015;1(3):e1500248.
|
CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)
pV1025/pV1093
|
| pV1393 | CaCas9/sgRNA-BsmBI stuffer (Synthetic) | | Ampicillin |
Fink
| New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
pV1390
|
| pV1539 | CaCas9/sgRNA-BsmBI stuffer (Synthetic) | | Ampicillin |
Fink
| New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
Genedrive entry vector for Candida albicans
pV1524
|
| pV1326 | CaCas9/sgRNA-BsmBI stuffer (Synthetic) | | URA3 ; clonNAT |
Fink
| New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR
pRS416
|
| pV1382 | CaCas9/sgRNA-BsmBI stuffer (Synthetic) | | URA3 ; clonNAT |
Fink
| New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR
pRS416
|
| pV1464 | CaCas9/sgRNA-BsmBI stuffer (Synthetic) | | URA3 ; clonNAT |
Fink
| New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
|
N. castellii Solo CRISPR vector, marked with URA3 and NatR
pRS416
|
| pZS157 CRISPEY RT/Cas9 | SpCas9, Ec86-RT (Other) | GAL1-GAL10 | HIS3 |
Fraser
| Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing.
Cell. 2018 Sep 18. pii: S0092-8674(18)31118-8. doi: 10.1016/j.cell.2018.08.057.
|
Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression
pRS403
|
| eGFP L202 gRNA | eGFP L202 gRNA (Other) | U6 | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.
MLM3636
|
| eGFP L138 gRNA | eGFP L138 gRNA (Other) | U6 | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.
MLM3636
|
| pAH235 | humanized Streptococcus pyogenes Cas9 (Synthetic) | nmt41 | S.pombe ura4 |
Tanaka
| Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976.
|
Cas9 expression controlled by the nmt41 promoter
pSLF273
|
| pAH237 | gRNA cassette (Schizosaccharomyces pombe), humanized Streptococcus pyogenes Cas9 (Schizosaccharomyces pombe) | S.pombe rrk1, nmt41 | LEU2 |
Tanaka
| Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976.
|
Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast
pAH233
|
| pAH243 | humanized Streptococcus pyogenes Cas9 (Schizosaccharomyces pombe), gRNA expression module (Schizosaccharomyces pombe) | nmt41, S.pombe rrk1 | S.pombe ura4 |
Tanaka
| Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976.
|
Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast
pSLF273
|
| pUCC001 | HH-BsaI (Synthetic) | TDH3 promoter from S.cerevisiae (already present in backbone) | Hygromycin |
Morrissey
| Biological Parts for Kluyveromyces marxianus Synthetic Biology
Front. Bioeng. Biotechnol., 07 May 2019
|
Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
pUDP002
|
| pSDMA65 | Hygromycin (HYG) (Synthetic) | ACT1 | Hygromycin |
Fraser
|
Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.
(unpublished)
|
Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.
pBlueScript II SK(-)
|
CRISPR Plasmids: Yeast