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CRISPR Plasmids: Mammalian Expression


The following CRISPR plasmids have been designed for use in mammalian expression systems.

Cut

Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.

To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower efficiency than NHEJ.

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
hCas9Cas9CMV Church RNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3. Expresses human codon optimized Cas9 nuclease for genome engineering pcDNA3.3-TOPO
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-purohumanized S. pyogenes Cas9Puromycin Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA. pUC ori vector
pX330-U6-Chimeric_BB-CBh-hSpCas9humanized S. pyogenes Cas9CBh Zhang Multiplex Genome Engineering Using CRISPR/Cas Systems. Science. 2013 Jan 3. A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid. pUC ori vector
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • pMJ920Cas9 (Synthetic)CMV Doudna RNA-programmed genome editing in human cells. elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29. MacroLab 6D
    JDS246mammalian codon-optimized streptococcus pyogenes Cas9 - 3X FlagCMV Joung Joung lab unpublished CRISPR plasmids (unpublished) Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters unknown
    p3s-Cas9HCCas9CMV Kim Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2507. pCDNA3.1
    pCas9_GFPCas9-2A-GFP (Synthetic)CAG Musunuru Enhanced efficiency of human pluripotent stem cell genome editing through replacing TALENs with CRISPRs. Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 10.1016/j.stem.2013.03.006. Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells pCAG
    pST1374-NLS-flag-linker-Cas9Human codon optimized Cas9CMVBlasticidin Huang Generation of gene-modified mice via Cas9/RNA-mediated gene targeting. Cell Res. 2013 Apr 2. doi: 10.1038/cr.2013.46. pST1374 (Addgene #13426)
    pSimpleII-NLS-NmCas9-HA-NLS(s)NmCas9 (Other)EF1a Thomson Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. This plasmid encode NmCas9 with two NLS and a HA tag pSimpleII
    pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)NmCas9 (Other), U6pr-tracrRNA (Other)EF1a, U6 Thomson Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter. pSimpleII
    pSimpleII-NmCas9-FLAGNmCas9 (Other) Thomson Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. FLAG tagged NmCas9 with no NLS. pSimpleII
    pSpCas9 (PX165)hSpCas9 (Synthetic)Cbh Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS) PX165
    pSpCas9(BB)-2A-GFP (PX458)hSpCas9 (Synthetic)Cbh Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA PX458
    pSpCas9(BB)-2A-Puro (PX459)hSpCas9-2A-Puro (Synthetic)CbhPuromycin Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988. PX459
    pCAG-T3-hCAS-pACodon optimized Cas9 (Synthetic)CAG promoter Fujii Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res. 2013 Aug 30. Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys pCAGGS
    M-SPcasCas9 (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian S. pyogenes Cas9 expression, human optimized pcDNA3.3 TOPO
    M-ST1casCas9 (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian S. thermophilus #1 Cas9 expression, human optimized pcDNA3.3 TOPO
    M-NMcasCas9 (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian N. meningitidis Cas9 expression, human optimized pcDNA3.3 hygro
    pCW-Cas9humanized S. pyogenes Cas9Tet ONPuromycin Sabatini Genetic screens in human cells using the CRISPR-Cas9 system. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. Doxycycline-inducible lentiviral expression of SpCas9 pCW57.1
    pCAG-hCas9hCas9CAGNeomycin (select with G418) Hatada Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system. Int J Mol Sci. 2013 Sep 30;14(10):19774-81. doi: 10.3390/ijms141019774. Expresses hCas9 under the CAG promoter for CRISPR pEGFP-N1
    lentiCRISPR v2Cas9 (Synthetic), Puromycin resistanceEFS-NS, EFS-NSPuromycin Zhang Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone. Custom
    lentiCas9-BlastCas9 (Synthetic), Blasticidin resistanceEFS-NS, EFS-NSBlasticidin Zhang Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone. pFUGW
    pLenti-OC-IRES-BSDOCT1 (Homo sapiens), Cas9 (Other)CMVBlasticidin Wei High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature. 2014 Apr 9. doi: 10.1038/nature13166. To create stable cell clones with high-level expression of Cas9 and OCT1 pLenti-CMV-BSD POU2F1 OCT1, OTF1, oct-1B
    pLV hUbC-Cas9-T2A-GFPhumanized Cas9 T2A GFP (Other)hUbC Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. 3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP FUGW
    pSQT834Csy4-T2A-Cas9-NLS (Homo sapiens)CAG Joung Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. Csy4 and Cas9 nuclease expression plasmid pCAG-CFP
    pSQT817Cas9CAG Joung Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. wildtype Cas9 expression plasmid pCAG-CFP
    pL-CRISPR.EFS.GFPSpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-eGFP (Synthetic)EFS, hU6 Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven pLKO.005
    pL-CRISPR.EFS.tRFPSpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-tRFP (Synthetic) Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven pLKO.005
    pLKO5d.EFS.SpCas9.P2A.BSDSpCas9 (Other), EFS (Homo sapiens), P2A-BSD (Synthetic)Blasticidin Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven pLKO.005
    pL-CRISPR.SFFV.tRFPSpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-tRFP (Synthetic) Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven pLKO.005
    pL-CRISPR.SFFV.GFPSpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-eGFP (Synthetic) Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven pLKO.005
    pL-CRISPR.EFS.PACSpCas9 (Other), Sp sgRNA scaffold, EFS (Homo sapiens), P2A-PAC (Synthetic)Puromycin Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven pLKO.005
    pL-CRISPR.SFFV.PACSpCas9 (Other), Sp sgRNA scaffold, SFFV (Synthetic), P2A-PAC (Synthetic)Puromycin Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven pLKO.005
    pAdSh.PGK.Cas9Human codon-optimized S. pyogenes Cas9 (Synthetic)Neomycin (select with G418) Goncalves Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter pBR322-based pAdEasy backbone
    pLKO5d.EFS.SpCas9.P2A.PACSpCas9 (Other), EFS (Homo sapiens), P2A-PAC (Synthetic)Puromycin Ebert Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven pLKO.005
    Puro-Cas9 donorhSpCas9 (Other)Puromycin Huangfu An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus N/A
    pX330A-1x2humanized S. pyogenes Cas9 nuclease (Other)CBh Yamamoto Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. Expresses Cas9 nuclease and gRNA pUC ori vector
    piCRg EntryhSpCas9 Huangfu An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. Cas9/gRNA Entry expression plasmid Low copy
    pCAG-T3-hCASeGFP-pACAS9 (Synthetic)CAG Fujii Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res. 2013 Aug 30. Expresses CAS9-eGFP fusion protein in mammalian cells. pCAGGS
    Eef1a-1955/-1>nls::Cas9::nlsnls::Cas9::nls (Other)Ciinte.Eef1a (EF1alpha) -1955/-1 Christiaen Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Eef1a (EF1alpha) promoter driving nls::Cas9::nls pCESAx
    Mesp-1916/-1>nls::Cas9::nlsnls::Cas9::nls (Other)Ciinte.Mesp -1916/-1 Christiaen Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Mesp driver driving nls::Cas9::nls pCESAx
    Sox1/2/3-2373/+3>nls::Cas9::nlsnls::Cas9::nls (Other)Ciinte.Sox1/2/3 (SoxB1) -2373/+3 Christiaen Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls pCESAx
    pHL-EF1a-SphcCas9-iP-ACRISPR Cas9 (Other)Puromycin Hotta Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 10.1016/j.stemcr.2014.10.013. Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene. pHL-EF1a-GW-iP-A
    pSECCCas9 (Other), CreEFS, EFS (after Cas9-2A) Jacks Rapid modelling of cooperating genetic events in cancer through somatic genome editing. Nature. 2014 Oct 22. doi: 10.1038/nature13906. 3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre pLL3.3
    PX551SpCas9 (Synthetic)pMecp2 Zhang In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055. pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter. pAAV
    LSL-Cas9-Rosa26TVCas9 (Synthetic), EGFPCAG, CAGNeomycin (select with G418) ; DTA Zhang CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Sep 24. pii: S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014. Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection. Ai9
    pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNAhSaCas9 (Other), Chimeric guide for SaCas9 (Other) Zhang In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. pAAV
    pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpAhSaCas9 (Other) Zhang In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9). pAAV
    pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNAhSaCas9 (Other), Chimeric guide for SaCas9 (Other) Zhang In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 1. doi: 10.1038/nature14299. A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA. pAAV
    pMuLE ENTR CMV-hCas9 R4-R3human codon optimized Cas9 (Synthetic)CMV Frew A versatile modular vector system for rapid combinatorial mammalian genetics. J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning N/A
    pMuLE ENTR SV40-hCas9 L3-L2human codon optimized Cas9 (Synthetic)SV40 Frew A versatile modular vector system for rapid combinatorial mammalian genetics. J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning N/A
    pMuLE ENTR SV40-hCas9 L5-L2human codon optimized Cas9 (Synthetic)SV40 Frew A versatile modular vector system for rapid combinatorial mammalian genetics. J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743. MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning N/A
    c3GIC9humanized S. Pyogenes Cas9 (Other)TRE3G Dow Inducible in vivo genome editing with CRISPR-Cas9. Nat Biotechnol. 2015 Apr;33(4):390-4. doi: 10.1038/nbt.3155. Epub 2015 Feb 18. col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes col1a1 Flp-in targeting construct
    ptreCas9-mKate2ps-T1gRNACas9–mKate2ps (Synthetic) Bleris CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18. Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA pTag-CFP
    pCas9–mKate2ps–EgRNACas9–mKate2ps (Synthetic) Bleris CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18. Empty; no U6 gRNA cassette pTag-CFP
    pCas9–mKate2ps–T1gRNACas9–mKate2ps (Synthetic) Bleris CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18. t1 gRNA (ATGAGAATCAAGGCGGTCGA) pTag-CFP
    PX851SpCas9 (aa 2-535)CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4). PX330
    PX852SpCas9 (aa536-1368)CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4). PX330
    PX853SpCas9 (aa 2-573)CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5). PX330
    PX854SpCas9 (aa573-1368)CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5). PX330
    pLSC-5SpCas9(574-1368), SpCas9(2-573)EFS, EFSPuromycin Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. Lenti vector for expression of inducible split Cas9 lentiCRISPR
    pSpCas9(BB)-2A-Puro (PX459) V2.0hSpCas9-2A-Puro V2.0 (Synthetic)CbhPuromycin Zhang Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0) PX459
    lentiCas9-EGFPCas9 (Synthetic), EGFP (Other)EFS-NS, EFS-NS Zhang Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. Epub 2015 Mar 5. Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone. pFUGW
    pX330S-2-PITChhumanized S. pyogenes Cas9 (Other)CBh Yamamoto MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. Expresses Cas9 nuclease and the PITCh-gRNA pUC ori vector
    pX330A-FBL/PITChhumanized S. pyogenes Cas9 (Other)CBh Yamamoto MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems. Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA pUC ori vector
    Adeno Cas9U6 and CBh Ventura In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. Adenoviral vector for the expression of the Cas9 pacAd5
  • Tag / Fusion Protein
    • FLAG
  • pX333CBh; U6 Ventura In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter. pX333
  • Tag / Fusion Protein
    • 3XFLAG-Cas9 (N terminal on backbone)
  • pKMD106e - intein-Cas9(S219)mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG (Other)CMV Liu Small molecule-triggered Cas9 protein with improved genome-editing specificity. Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6. Expresses intein-Cas9(S219) in mammalian cells pJDS246
    pKMD106h - intein-Cas9(C574)mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG (Other)CMV Liu Small molecule-triggered Cas9 protein with improved genome-editing specificity. Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6. Expresses intein-Cas9(C574) in mammalian cells pJDS246
    pKMD111e - intein-Cas9(S219-G521R)mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG (Other)CMV Liu Small molecule-triggered Cas9 protein with improved genome-editing specificity. Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. Epub 2015 Apr 6. Expresses intein-Cas9(S219-G521R) in mammalian cells pJDS246
    pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)Cas9 (Other), hU6 promoter; BbsI sites for sgRNA, H1 promoter; BamHI site for shRNACBh, U6, H1 Kuehn Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A pU6-(BbsI)_CBh-Cas9-T2A-BFP
    pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1BCas9 (Other), sgRNA cassetteCBh, U6 Kuehn Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A pU6-(BbsI)_CBh-Cas9-T2A-BFP
    pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1BCas9 (Other), sgRNA targeting ROSA26-1CBh, U6 Kuehn Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
    pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6Cas9 (Other), sgRNA cassetteCBh, U6 Kuehn Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A pU6-(BbsI)_CBh-Cas9-T2A-BFP
    pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1BCas9 (Other), sgRNA cassetteCBh, U6 Kuehn Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A pU6-(BbsI)_CBh-Cas9-T2A-mCherry
    pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6Cas9 (Other), sgRNA cassetteCBh, U6 Kuehn Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A pU6-(BbsI)_CBh-Cas9-T2A-mCherry
    pU6-(BbsI)_CBh-Cas9-T2A-BFPCas9 (Other), sgRNA cassetteCBh, U6 Kuehn Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide pX330
    pU6-(BbsI)_CBh-Cas9-T2A-mCherryCas9 (Other), sgRNA cassetteCBh, U6 Kuehn Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide pX330
    pMCS-rybozyme-IRES-CAS9CAS9 (Synthetic), HDV ribozyme (Other) Fujii Development of a mono-promoter-driven CRISPR/Cas9 system in mammalian cells. Sci Rep. 2015 Dec 16;5:18341. doi: 10.1038/srep18341. Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9. pCAGGS
    pSaCas9_GFPSaCas9-2A-GFP (Synthetic)CAG Musunuru Staphylococcus aureus Cas9 (unpublished) Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells pCAG
    pCas9–mKate2ps–CgRNACas9–mKate2ps Bleris CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells. Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18. Control gRNA (GTCAAGGCACTCTTGCCTA) pTag-CFP
    MSCV_Cas9_puroCas9 (Synthetic)MSSV_LTRPuromycin Vakoc Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains. Nat Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235. Retroviral introduction of Cas9 into mammalian cell line. MSCV_puro
    MSP469mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag (Other)CMV & T7 Joung Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015 Jun 22. doi: 10.1038/nature14592. Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant) JDS246
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x FLAG (C terminal on insert)
  • MSP680mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag (Other)CMV & T7 Joung Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015 Jun 22. doi: 10.1038/nature14592. Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant) JDS246
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x FLAG (C terminal on insert)
  • MSP1101mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag (Other)CMV & T7 Joung Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015 Jun 22. doi: 10.1038/nature14592. Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant) JDS246
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x FLAG (C terminal on insert)
  • MSP977mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag (Other)CMV & T7 Joung Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015 Jun 22. doi: 10.1038/nature14592. Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG JDS246
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x FLAG (C terminal on insert)
  • MSP1594mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS (Other)CAG Joung Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015 Jun 22. doi: 10.1038/nature14592. Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS pCAG-CFP
  • Tag / Fusion Protein
    • NLS (C terminal on insert)
  • BPK2139mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag (Other)CAG Joung Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature. 2015 Jun 22. doi: 10.1038/nature14592. Human expression vector for S .aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG pCAG-CFP
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • 3x FLAG (C terminal on insert)
  • pBGKCas9 Ohtsuka Mouse Genome Editing Using the CRISPR/Cas System. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83. Vector for Cas9 mRNA synthesis pcDNA3.1
    pCAS9-mCherry-Frame +0gRNA frame +0 Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. CRISPaint frame selector +0 pCAS9-mCherry
    pCAS9-mCherry-Frame +1gRNA frame +1 Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. CRISPaint frame selector +1 pCAS9-mCherry
    pCAS9-mCherry-Frame +2gRNA frame +2 Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. CRISPaint frame selector +2 pCAS9-mCherry
    pCAS9-mCherry-TUBBgRNA TUBB Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. CRISPaint target selector TUBB pCAS9-mCherry
    pCAS9-mCherry-ACTG1gRNA ACTG1 (Homo sapiens) Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. CRISPaint target selector ACTG1 pCAS9-mCherry
    pCAS9-mCherry-HIST1H4CgRNA HIST1H4C (Homo sapiens) Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. CRISPaint target selector HIST1H4C pCAS9-mCherry
    pKLV2-EF1a-BsdCas9-WEF1a-Cas9 cassette, WPRE (Homo sapiens)EF1a Yusa A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus pKLV2 lentiviral vector
    pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-WU6gRNA cassette, PGKGFP2ABFP cassette, WPRE (Homo sapiens) Yusa A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter (control) with GFP and BFP pKLV2 lentiviral vector
    pRosa26-EF1a-hCas9IRESneoEF1a-hCas9IRESneopA (Synthetic) Yusa A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette pBluescriptII
    phumanCas9 (GB0575)Cas9 coding region (human codon optimised) (Other) Orzaez GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Plant Physiol. 2013 Jul;162(3):1618-31. Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part pUPD
    PX377 Corynebacter diphtheriae Cas9CdCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Corynebacter diphtheriae Cas9 pDREAM
    PX378 Sutterella wadsworthensis Cas9SwCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Sutterella wadsworthensis Cas9 pDREAM
    PX379 Legionella pneumophila str. Paris Cas9LpCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Legionella pneumophila str. Paris Cas9 pDREAM
    PX380 Treponema denticola Cas9TdCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Treponema denticola Cas9 pDREAM
    PX381 Filifactor alocis Cas9FaCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Filifactor alocis Cas9 pDREAM
    PX382 Staphylococcus pseudintermedius Cas9SpsCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Staphylococcus pseudintermedius Cas9 pDREAM
    PX383 Lactobacillus johnsonii Cas9LjCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Lactobacillus johnsonii Cas9 pDREAM
    PX385 Streptococcus pasteurianus Cas9SpaCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Streptococcus pasteurianus Cas9 pDREAM
    PX386 Lactobacillus farciminis Cas9LfCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Lactobacillus farciminis Cas9 pDREAM
    PX387 Mycoplasma mobile Cas9MmCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Mycoplasma mobile Cas9 pDREAM
    PX388 Bacteroides coprophilus Cas9BcCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Bacteroides coprophilus Cas9 pDREAM
    PX389 Fluviicola taffensis Cas9FtCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Fluviicola taffensis Cas9 pDREAM
    PX390 Flavobacterium columnare Cas9FcCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Flavobacterium columnare Cas9 pDREAM
    PX391 Sphaerochaeta globus str. Buddy Cas9SgCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Sphaerochaeta globus str. Buddy Cas9 pDREAM
    PX392 Azospirillum B510 Cas9AzoCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Azospirillum B510 Cas9 pDREAM
    PX393 Gluconacetobacter diazotrophicus Cas9GdCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Gluconacetobacter diazotrophicus Cas9 pDREAM
    PX394 Neisseria cinerea Cas9NcCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Neisseria cinerea Cas9 pDREAM
    PX395 Roseburia intestinalis Cas9RiCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Roseburia intestinalis Cas9 pDREAM
    PX396 Parvibaculum lavamentivorans Cas9PlCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Parvibaculum lavamentivorans Cas9 pDREAM
    PX398 Nitratifractor salsuginis str DSM 16511 Cas9NsCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Nitratifractor salsuginis str DSM 16511 Cas9 pDREAM
    PX399 Mycoplasma gallisepticum str. F Cas9MgCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Mycoplasma gallisepticum str. F Cas9 pDREAM
    PX400 Campylobacter lari CF89-12 Cas9ClCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Campylobacter lari CF89-12 Cas9 pDREAM
    PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9St3Cas9 (Synthetic)CBh Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Streptococcus thermophilus LMD-9 CRISPR 3 Cas9 Unknown
    PX404 Campylobacter jejuni Cas9CjCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Campylobacter jejuni Cas9 Unknown
    pKLV2-EF1a-Cas9Bsd-WEF1a-Cas9 cassette, WPRE (Homo sapiens)EF1a Yusa A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus pKLV2 lentiviral vector
    LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIRpuromycin (Synthetic), hCas9 (Synthetic)CAG, CAGPuromycin Elverløv-Jakobsen Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression Amp
    px330_SMAD exon 9 gRNASMAD exon 9 gRNA (Synthetic)U6 Elverløv-Jakobsen Cas9/CRISPR pig transposon plasmid_LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR (unpublished) px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter. amp
    CAS9PBKSCas9 (Other)CbAkt Bennett Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis. Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. Epub 2017 Feb 16. CbAkt promoter driven Cas9-2A-GFP (from px458) pBKS
    Cas9-2A-CreCas9 and Cre recombinase (Homo sapiens)CAG Zhang Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. co-expressing Cas9 and Cre recombinase Cas9-2A-GFP
    PX405 Neisseria meningitidis Cas9NmCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Neisseria meningitidis Cas9 pDREAM
    PX406 Pasteurella multocida Cas9PmCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Pasteurella multocida Cas9 pDREAM
    PX408 Francisella tularensis subsp. novicida Cas9FtCas9 (Synthetic)CMV Zhang Feng Zhang lab bacterial Cas9 orthologs (unpublished) Francisella tularensis subsp. novicida Cas9 pDREAM
    pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268Zif268 (Mus musculus) Wolfe DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 fused to Zif268 in mammalian cells pCSDest2-SpCas9-NLS-3XHA-NLS Egr1 A530045N19Rik, ETR103, Egr-1, Krox-1, Krox-24, Krox24, NGF1-A, NGFI-A, NGFIA, TIS8, Zenk, Zfp-6, Zif268, egr
    pCSDest2-SpCas9-NLS-3XHA-NLSSpCas9 (Other) Wolfe DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 in mammalian cells pCSDest2
    pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2TS2 ZFP (Synthetic) Wolfe DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells pCSDest2-SpCas9-NLS-3XHA-NLS
    pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3TS3 ZFP (Synthetic) Wolfe DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells pCSDest2-SpCas9-NLS-3XHA-NLS
    pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4TS4 ZFP (Synthetic) Wolfe DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells pCSDest2-SpCas9-NLS-3XHA-NLS
    pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3TS3 ZFP (Synthetic) Wolfe DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268
    pY004 (pcDNA3.1-hFnCpf1)hFnCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized FnCpf1 pcDNA3.1
    pY005 (pcDNA3.1-hLb3Cpf1)hLb3Cpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized Lb3Cpf1 pcDNA3.1
    pY006 (pcDNA3.1-hBpCpf1)hBpCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized BpCpf1 pcDNA3.1
    pY007 (pcDNA3.1-hPeCpf1)hPeCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized PeCpf1 pcDNA3.1
    pY008 (pcDNA3.1-hPbCpf1)hPbCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized LPbCpf1 pcDNA3.1
    pY009 (pcDNA3.1-hSsCpf1)hSsCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized SsCpf1 pcDNA3.1
    pY010 (pcDNA3.1-hAsCpf1)hAsCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized AsCpf1 pcDNA3.1
    pY011 (pcDNA3.1-hLb2Cpf1)hLb2Cpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized Lb2Cpf1 pcDNA3.1
    pY012 (pcDNA3.1-hCMtCpf1)hCMtCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized CMtCpf1 pcDNA3.1
    pY013 (pcDNA3.1-hEeCpf1)hEeCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized EeCpf1 pcDNA3.1
    pY014 (pcDNA3.1-hMbCpf1)hMbCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized MbCpf1 pcDNA3.1
    pY015 (pcDNA3.1-hLiCpf1)hLiCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized LiCpf1 pcDNA3.1
    pY016 (pcDNA3.1-hLbCpf1)hLbCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized LbCpf1 pcDNA3.1
    pY017 (pcDNA3.1-hPcCpf1)hPcCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized PcCpf1 pcDNA3.1
    pY018 (pcDNA3.1-hPdCpf1)hPdCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized PdCpf1 pcDNA3.1
    pY019 (pcDNA3.1-hPmCpf1)hPmCpf1 (Other)CMVNeomycin (select with G418) Zhang Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. Expresses humanized PmCpf1 pcDNA3.1
    FUCas9Cherryhumanised S.pyogenes cas9 (Other)hUbC Herold An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo. Cell Rep. 2015 Mar 3;10(8):1422-32. doi: 10.1016/j.celrep.2015.02.002. Epub 2015 Feb 26. Expresses Cas9 with fluorescent Cherry reporter FUGW
    lentiCas9-VenusCas9 (Synthetic), Venus Reporter (Synthetic)EFS-NS, P2AVenus Bauer BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Nature. 2015 Sep 16. doi: 10.1038/nature15521. Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone. pFUGW
    MSP1830mammalian codon-optimized KKH variant Staphylococcus aureus Cas9(E782K/N968K/R1015H)-NLS-3xFlag (Other)CAG Joung Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition. Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404. Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG pCAG-CFP
    spCas9-BlastR (pCBhCas9-BlastR)spCas9CBhBlasticidin Sherwood Cloning-free CRISPR. Stem Cell Reports. 2015 Oct 27. pii: S2213-6711(15)00284-2. doi: 10.1016/j.stemcr.2015.09.022. Expresses SpCas9 and Blasticidin resistance p2Tol2
    pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)humanized S. pyogenes Cas9 fused to human Geminin (Homo sapiens)CBh Draetta Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair. Cell Rep. 2016 Feb 3. pii: S2211-1247(16)00040-1. doi: 10.1016/j.celrep.2016.01.019. A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 GMNN Gem, MGORS6
    eSpCas9(1.1)enhanced specificity Cas9 (1.1) (Other)CBh Zhang Rationally engineered Cas9 nucleases with improved specificity. Science. 2015 Dec 1. pii: aad5227. Expresses high specificity SpCas9. Px330-like plasmid. pX330-U6-Chimeric_BB-CBh-hSpCas9
    pCR1002T7pr_10xHis-MBP-TEV-SPyCas9 Corn Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481. Express Streptococcus pyogenes Cas9 SPynCas9
    VP12mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag (Other)CMV Joung High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016 Jan 6. doi: 10.1038/nature16526. Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG JDS246
    MSP2135mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag (Other)CMV Joung High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016 Jan 6. doi: 10.1038/nature16526. Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG JDS246
    MSP2133mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag (Other)CMV Joung High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016 Jan 6. doi: 10.1038/nature16526. Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG JDS246
    MSP2440mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag (Other)CMV Joung High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016 Jan 6. doi: 10.1038/nature16526. Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG JDS246
    BPK2797mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag (Other)CMV Joung High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016 Jan 6. doi: 10.1038/nature16526. Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG JDS246
    MSP2443mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag (Other)CMV Joung High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016 Jan 6. doi: 10.1038/nature16526. Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG JDS246
    pCas9-polyACas9-polyadenine (Other)T7 Mashimo ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431. express hCas9 byT7 promoter with polyadenine tails pcDNA3.3-TOPO
    MSP2372mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag (Other)CMV Joung High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. Nature. 2016 Jan 6. doi: 10.1038/nature16526. Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG JDS246
    Lenti‐Cas9‐2A‐BlastCas9 (Synthetic)EFS-NSBlasticidin Moffat High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter lenti CRISPR pXPR_001
    pAAVS1-PDi-CRISPRnCas9 (Synthetic), rtTA (Synthetic)TRE, CAGPuromycin Conklin CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10. Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus pAAVS1
    pAWp30EFSp-Cas9-P2A-Zeo Lu Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM. Proc Natl Acad Sci U S A. 2016 Feb 10. pii: 201517883. pFUGW-EFSp-Cas9-P2A-Zeo pFUGW
    pTE4396As crRNA, AsCpf1human U6, CMVNeomycin (select with G418) Welker Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0. Expresses human codon-optimized AsCpf1 and As crRNA. pcDNA3.1
    pTE4398Lb crRNA, LbCpf1human U6, CMVNeomycin (select with G418) Welker Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0. Expresses human codon-optimized LbCpf1 and Lb crRNA. pcDNA3.1
    pBLO1811_Cas9_noNLS_humanCas9 (Homo sapiens) Savage Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA px330
    pBLO1806_Cas9_2xNLS_humanCas9 (Homo sapiens) Savage Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA px330
    pLentiCRISPR-EGFP Bornhauser Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986. Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance lentiCRISPRv1
  • Tag / Fusion Protein
    • EGFP
  • pLentiCRISPR-tagBFP Bornhauser Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986. Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance lentiCRISPRv1
  • Tag / Fusion Protein
    • tagBFP
  • pLentiCRISPR-mCherry Bornhauser Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986. Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation. lentiCRISPRv1
  • Tag / Fusion Protein
    • mCherry
  • pLentiCRISPR-RFP657 Bornhauser Activation of concurrent apoptosis and necroptosis by SMAC mimetics for the treatment of refractory and relapsed ALL. Sci Transl Med. 2016 May 18;8(339):339ra70. doi: 10.1126/scitranslmed.aad2986. Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance lentiCRISPRv1
  • Tag / Fusion Protein
    • RFP657
  • FUG-T2A-Cas9Cas9Ubiquitin Luikart A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons. Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611. Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL. FUGW
    pRubiC-T2A-Cas9Cas9Ubiquitin Luikart A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons. Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611. Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL. pRubi
    pRubiG-T2A-Cas9Cas9Ubiquitin Luikart A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons. Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611. Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL. pRubi
    PXLCas9U6 Luikart A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons. Sci Rep. 2016 May 10;6:25611. doi: 10.1038/srep25611. PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA. PX330
    pX330-BsaIx2-DD-Cas9DD-SpCas9 (Other)CAG Calos In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining. Nucleic Acids Res. 2016 Jan 13. pii: gkv1542. Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1 modified pX330
    pCas9-VRER_2A_GFPCas9-VRER (Synthetic)CAG Tessier-Lavigne Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9. Nature. 2016 May 5;533(7601):125-9. doi: 10.1038/nature17664. Epub 2016 Apr 27. Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control pCAG
    pXPR_BRD111Cas9Blasticidin Hahn Integrated genetic and pharmacologic interrogation of rare cancers. NATURE COMMUNICATIONS 7:11987 Expresses Cas9 with the pLX311 backbone pLX311
    CAG-Cas9-T2A-EGFP-ires-puroWT SpCas9-T2A-EGFP-ires-puromycin resistance (Other)CAGPuromycin Otonkoski An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation. Cell Rep. 2017 Apr 11;19(2):281-294. doi: 10.1016/j.celrep.2017.03.055. Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter. PyCAG
    pLentiCas9-T2A-BFPT2A-BFPIn frame with Cas9Blasticidin Guigo Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion. PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 10.1371/journal.pcbi.1005341. eCollection 2017 Mar. Cas9 protein with BFP Lenti-Cas9-blast
    pLentiCas9-T2A-GFPT2A-GFPIn frame with Cas9Blasticidin Guigo Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion. PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 10.1371/journal.pcbi.1005341. eCollection 2017 Mar. Cas9 protein with GFP Lenti-Cas9-blast
    pZac2.1 SV40-CMV-SaCas9-3xNLSSV40-CMV-SaCas9-3xNLS (Synthetic)SV40, CMV promoters Wagers In vivo gene editing in dystrophic mouse muscle and muscle stem cells. Science. 2016 Jan 22;351(6271):407-11. doi: 10.1126/science.aad5177. Epub 2015 Dec 31. AAV vector containing SaCas9 pZac2.1
    SQT1659mammalian codon-optimized Acidaminococcus sp. BV3L6 Cpf1-NLS-3xHA (Other)CAG Joung Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. Human expression plasmid for AsCpf1-NLS-3xHA pCAG-GFP
    SQT1665mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA (Other)CAG Joung Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. Human expression plasmid for LbCpf1-NLS-3xHA pCAG-GFP
    pLentiCRISPR-EPuromycin Abbosh LentiCRISPR version E (unpublished) Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette pLentiCRISPRV2
  • Tag / Fusion Protein
    • Cas9-P2A-Puro
  • pCAG-SpCas9-GFP-U6-gRNASpCas9CAG Zou The ribosomal prolyl-hydroxylase OGFOD1 decreases during cardiac differentiation and modulates translation and splicing. JCI Insight. 2019 May 21;5. pii: 128496. doi: 10.1172/jci.insight.128496. All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC pSpCas9(BB)-2A-GFP (PX458)
    pCAG-eCas9-GFP-U6-gRNAeSpCas9(1.1)CAG Zou pCAG-SpCas9-U6-gRNA (unpublished) All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells eSpCas9(1.1)
    eSpCas9(1.1)_No_FLAGCBh Doyon A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 10.1016/j.celrep.2015.09.009. FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid. pX330-U6-Chimeric_BB-CBh-hSpCas9
  • Tag / Fusion Protein
    • Untagged eSpCas9(1.1)
  • pTE4495Mb crRNA (Other), MbCpf1human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. mammalian expression MbCpf1 nuclease and Mb crRNA pcDNA3.1
    pTE4497Fn crRNA (Other), FnCpf1human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. mammalian expression FnCpf1 nuclease and Fn crRNA pcDNA3.1
    pDNR-SpCas9-GemSpCas9-Gem (Other)T7 Little A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4. Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining pDNR-Dual
    pDNR-SpCas9SpCas9 (Other)T7 Little A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4. Used for in vitro transcription of SpCas9 mRNA. pDNR-Dual
    pDNR-SpCas9-Cdt1SpCas9-Cdt1 (Other) Little A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Stem Cell Reports. 2016 Sep 13;7(3):508-17. doi: 10.1016/j.stemcr.2016.07.001. Epub 2016 Aug 4. Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle. pDNR-Dual
    peSpCas9(1.1)-2×sgRNA (empty, donor) Nakayama Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system. Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051. All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty. eSpCas9(1.1)
    pAAV-SMVP-Cas9NCas9N (Synthetic) Church A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. Expresses Cas9N in mammalian cells. pZac2.1
    pAAV-SMVP-Cas9CCas9C (Synthetic) Church A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. Expresses Cas9C in mammalian cells. pZac2.1
    pAAV-CASI-Cas9CCas9C (Synthetic) Church A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. Expresses Cas9C in mammalian cells; derived from pZac2.1 with CASI promoter. pZac2.1
    pSMVP-Cas9NCas9N (Synthetic) Church A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. Plasmid that expresses Cas9N in mammalian cells. pMAX
    pSMVP-Cas9C-P2A-turboGFPCas9C (Synthetic) Church A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP. pMAX
    pSMVP-Cas9CCas9C (Synthetic) Church A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. Plasmid that expresses Cas9C in mammalian cells. pMAX
    pSMVP-Cas9FL-P2A-turboGFPCas9FL (Synthetic) Church A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP. pMAX
    pAAV-CASI-Cas9C-P2A-turboGFPCas9C (Synthetic) Church A multifunctional AAV-CRISPR-Cas9 and its host response. Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993. Expresses Cas9C in mammalian cells; co-expresses turboGFP. pZac2.1
    pRZ-CAS9-mCherryCas9 (Synthetic)CMV Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. Cas9, mCherry expression pRZ
    pCAS9-mCherry empty Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. Cas9, mCherry, and sgRNA expression pCAS9
    pCAS9-BFP empty Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. Cas9, TagBFP, and sgRNA expression pCAS9
    Construct 7 - CMVp-Cas9-3xNLS-HSVpACas9-3xNLS (Homo sapiens) Lu Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Science. 2016 Aug 18. pii: aag0511. Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression pGL2-Luc (Addgene #26280)
    Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroRCas9-3xNLS (Homo sapiens)Puromycin Lu Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Science. 2016 Aug 18. pii: aag0511. Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection Lentiviral
    Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroRCas9-3xNLSPuromycin Lu Continuous genetic recording with self-targeting CRISPR-Cas in human cells. Science. 2016 Aug 18. pii: aag0511. Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection Lentiviral
    LentiCRISPRv2CreCre Recombinase (Other)EFS (P2A) Feldser Systematic in vivo inactivation of chromatin regulating enzymes identifies Setd2 as a potent tumor suppressor in lung adenocarcinoma. Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 10.1158/0008-5472.CAN-16-2159. Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site lentiCRISPR v2 (Addgene #52961)
    LentiCRISPRv2GFPGFP (Other)EFS (P2A) Feldser Systematic in vivo inactivation of chromatin regulating enzymes identifies Setd2 as a potent tumor suppressor in lung adenocarcinoma. Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 10.1158/0008-5472.CAN-16-2159. 3rd generation lentiviral vector expressing GFP alongside Cas9 and an sgRNA cloning site lentiCRISPR v2
    lentiCRISPR v2-BlastBlasticidin S deaminaseEF-1αBlasticidin Babu CRISPR/Cas9-based system for loss-of-function screening (unpublished) Mammalian expression of Cas9 and sgRNA scaffold lentiCRISPR v2 (#52961)
    pCW-Cas9-BlastSpCas9, Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivatorTight Tre, human phosphoglycerate kinase 1 promoterBlasticidin Babu CRISPR/Cas9-based system for loss-of-function screening (unpublished) Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminaase -T2A -rtTA pCW-Cas9 (#50661)
    pLV-U6-gRNA-UbC-DsRed-P2A-BsrDsRed-Express2, BsrhUbCBlasticidin Gersbach CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR FUGW
    pLV-U6-gRNA-UbC-eGFP-P2A-BsreGFP, BsrhUbCBlasticidin Gersbach CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR FUGW
    pPB-US-ECasEERT2-spCas9-ERT2 (Other)U6 (gRNA), EF1a (ERT2-Cas9-ERT2)Puromycin Chen Chen lab CRISPR plasmids (unpublished) Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette. Piggybac Transposon
    pCRISPR-S12hSpCas9 (Other), eGFP (Other)CMV, CMV (downstream of F2A self-cleaving peptide)Puromycin, Hygromycin Wu An episomal CRISPR/Cas9 system to derive vector-free gene modified mammalian cells. Protein Cell. 2016 Sep;7(9):689-91. doi: 10.1007/s13238-016-0299-9. To episomally express codon optimized Cas9 and chimeric guide RNA pCEP4
    pX601-mCherrymCherryT2A Kan Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia. Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6. Staphylococcus aureus (SaCas9) conjugated with mCherry pX601
    pX601-GFPGFP (Other) Kan Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and beta-thalassemia. Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 10.1073/pnas.1612075113. Epub 2016 Sep 6. Staphylococcus aureus (SaCas9) conjugated with GFP pX601
    iCasCas9 (Other)CMVOFP expression Tan A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing. Nat Chem Biol. 2016 Sep 12. doi: 10.1038/nchembio.2179. Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT) GeneArt CRISPR Nuclease vector with OFP reporter
    pY108 (lenti-AsCpf1)huAsCpf1 (Synthetic), puromycin resistance gene (Synthetic)EFSPuromycin Zhang Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Lenti virus delivery of AsCpf1 and crRNA guide pHKO_23
    pY109 (lenti-LbCpf1)huLbCpf1 (Synthetic), puromycin resistance gene (Synthetic)EFSPuromycin Zhang Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Lenti virus delivery of LbCpf1 and crRNA guide pHKO_23
    pY026huAsCpf1 (Synthetic)CMV Zhang Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Expresses huAsCpf1 and crRNA guide pcDNA3.1
    pY027huLbCpf1 (Synthetic)CMV Zhang Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Expresses huLbCpf1 and crRNA guide pcDNA3.1
    pY094huAsCpf1 (Synthetic), GFP (Synthetic)CMV Zhang Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Expresses huAsCpf1-T2A-GFP and crRNA guide pcDNA3.1
    pY095huLbCpf1 (Synthetic), GFP (Synthetic)CMV Zhang Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Expresses huLbCpf1-T2A-GFP and crRNA guide pcDNA3.1
    pY30huAsCpf1 (Synthetic), puromycin resistance gene (Synthetic)CMVPuromycin Zhang Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Expresses huAsCpf1-P2A-puro and crRNA guide pcDNA3.1
    pY31huLbCpf1 (Synthetic), puromycin resistance gene (Synthetic)CMVPuromycin Zhang Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5. Expresses huLbCpf1-P2A-puro and crRNA guide pcDNA3.1
    Lenti-AsCpf1-BlastAsCpf1 (Other)EFS-NSBlasticidin Kim In vivo high-throughput profiling of CRISPR-Cpf1 activity. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. lentiCas9-Blast(Addgene#:52962)
    Lenti-LbCpf1-BlastLbCpf1 (Other)EFS-NSBlasticidin Kim In vivo high-throughput profiling of CRISPR-Cpf1 activity. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. lentiCas9-Blast(Addgene#:52962)
    pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova) loxP-stop-loxP (Other) Iwasato Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9. pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene Plasmid #42230)
    Lenti-iCas9-neoFlag-iCas9-P2A-GFP (Other)Neomycin (select with G418) Yan An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting. Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25. Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence pInducer-20
    Lenti-multi-CRISPRPuromycin Yan An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting. Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25. Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression Lenti-CRISPR
    pAAV-RSV-SpCas9pRSV (Other)pRSV Lei The CRISPR/Cas9-created MDM2 T309G enhances vitreous-induced expression of MDM2 and proliferation and survival of cells. J Biol Chem. 2016 May 31. pii: jbc.M116.729467. Express SpCas9 in mammalian cells pAAV
    pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPREU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro (Synthetic)Puromycin Regev A Distinct Gene Module for Dysfunction Uncoupled from Activation in Tumor-Infiltrating T Cells. Cell. 2016 Sep 8;166(6):1500-1511.e9. doi: 10.1016/j.cell.2016.08.052. Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker. pLKO
    FUCas9Cyanhumanised S.pyrogenes Cas9 (Other)hUBC Herold (unpublished) Expresses Cas9 with fluorescent Cyan reporter FUGW
    FUCas9GFPhumanised S.pyogenes Cas9 (Other)hUBC Herold (unpublished) Expresses Cas9 with green fluorescent reporter FUGW
    pLenti-Cas9-GFPCas9-GFP (Synthetic)EFS Sabatini Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. Lentiviral vector expressing Cas9-GFP pLentiCRISPR v1
    pY036_ATP1A1_G3_ArrayATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA (Other)CBh Doyon Marker-free coselection for CRISPR-driven genome editing in human cells. Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265. Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid pY036-U6-crRNA(BbsI)-CBh-AsCpf1
    pY036_ATP1A1_G5_ArrayATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA (Other)CBh Doyon Marker-free coselection for CRISPR-driven genome editing in human cells. Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265. Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid pY036-U6-crRNA(BbsI)-CBh-AsCpf1
    pAAV-Neo_CAG-Cas9Cas9 (Other)pCagNeomycin (select with G418) Vallier Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Development. 2016 Dec 1;143(23):4405-4418. Cas9 expression vector for targeting to the AAVS1 locus AAVS1-SA-2A-NEO-CAG-RTTA3 Addgene 60431
    Hygro-Cas9 donorhSpCas9 (Other)Hygromycin Huangfu Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Cell Stem Cell. 2017 Feb 8. pii: S1934-5909(17)30001-2. doi: 10.1016/j.stem.2017.01.001. Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection N/A
    pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpACas9-venus (Synthetic)CAG Kuehn Kuehn -sgRNA plasmids (unpublished) For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein Bluescript
    pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFPCas9 (Other)CAG Kuehn Kuehn -sgRNA plasmids (unpublished) For cloning and expression of sgRNA together with expression of Cas9 and TagRFP Bluescript
    pCAG-1BPNLS-Cas9-1BPNLS1BPNLS-Cas9-1BPNLS (Synthetic)CAG Belmonte In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16. BPNLS-Cas9-BPNLS expression plasmid pCAG
    pCAG-1BPNLS-Cas9-1BPNLS-2AGFP1BPNLS-Cas9-1BPNLS-2A-EGFP (Synthetic)CAG Belmonte In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16. BPNLS-Cas9-BPNLS-2AGFP expression plasmid pCAG
    pAAV-nEFCas9nEF-Cas9 (Synthetic) Belmonte In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16. SpCas9 expression AAV backbone plasmid under control of nEF promoter PX551
    lenti-Cas9-VQR-BlastSpCas9-VQR(D1135V,R1335Q,T1337R) (Other)Blasticidin Bauer Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793. lentiviral expression of SpCas9-VQR (NGA PAM restricted) lentiCas9-Blast
    P628_SpCas9-RecARecA (Synthetic)CBh Luo Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells. J Biotechnol. 2017 Mar 1;247:42-49. doi: 10.1016/j.jbiotec.2017.02.024. Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency PX459
    P823_eSpCas9(1.1)-RecARecACBh Luo Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells. J Biotechnol. 2017 Mar 1;247:42-49. doi: 10.1016/j.jbiotec.2017.02.024. Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency eSpCas9(1.1)
    TLCV2Cas9-2A-eGFPTight TRE promoter, Tight TRE promoterPuromycin Karpf Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer. Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression. lentiCRISPR v2
  • Tag / Fusion Protein
    • Cas9-T2A-eGFP
  • pCSDest2-NmeCas9-NLS-3XHA-NLSNme Cas9 (Other)CMV Sontheimer Naturally Occurring Off-Switches for CRISPR-Cas9. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Mammalian expression of wild type Nme Cas9 pCSDest2
    SIN-CMV-Ca9-WPREspCas9 (Other)CMV Déglon The self-inactivating KamiCas9 system for the editing of CNS disease genes Cell Reports , Volume 20 , Issue 12 , 2980 - 2991 Transfer plasmid for the production of lentiviral vectors SIN lentiviral transfer vector
    SIN-PGK-Cas9-V5-WPREspCas9 (Other)mouse PGK Déglon The self-inactivating KamiCas9 system for the editing of CNS disease genes Cell Reports , Volume 20 , Issue 12 , 2980 - 2991 To produce lentiviral vector for Cas9 editing SIN lentiviral transfer vector
    SIN-PGK-Cas9-WPREhCas9 (Other)mouse PGK Déglon The self-inactivating KamiCas9 system for the editing of CNS disease genes Cell Reports , Volume 20 , Issue 12 , 2980 - 2991 To produce Lentiviral vector for gene editing HIV-1 lentiviral SIN transfer vector
    SIN-CMV-Cas9-V5-WPRECas9-V5 (Other)CMV Déglon The self-inactivating KamiCas9 system for the editing of CNS disease genes Cell Reports , Volume 20 , Issue 12 , 2980 - 2991 To produce lentiviral vector for gene editing HIV-1 SIN lentiviral transfer vector
    pTE4254St chimeric gRNA, hStCas9human U6, CMVNeomycin (select with G418) Welker Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0. Expresses St chimeric gRNA and human codon-optimized StCas9 pcDNA3.3-TOPO
    pKS7107Nm crRNA, Nm tracrRNA, hNmCas9human U6, human U6, EF1a Welker Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0. Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9 pSimpleII
    pcDNA3.1-hAsCpf1(TYCV) (pY210)Acidaminococcus sp. Cpf1 (RR variant) (Synthetic)CMV Zhang Engineered Cpf1 variants with altered PAM specificities. Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. Expresses humanized AsCpf1 variant that recognizes TYCV PAMs pcDNA3.1
    pAsCpf1(TYCV)(BB) (pY211)Acidaminococcus sp. Cpf1 (RR variant) (Synthetic)CBh Zhang Engineered Cpf1 variants with altered PAM specificities. Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide pUC ori vector
    pcDNA3.1-hAsCpf1(TATV) (pY220)Acidaminococcus sp. Cpf1 (RVR variant) (Synthetic)CMV Zhang Engineered Cpf1 variants with altered PAM specificities. Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. Expresses humanized AsCpf1 variant that recognizes TATV PAMs pcDNA3.1
    pAsCpf1(TATV)(BB) (pY221)Acidaminococcus sp. Cpf1 (RVR variant) (Synthetic)CBh Zhang Engineered Cpf1 variants with altered PAM specificities. Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. Expresses humanized AsCpf1 TATV PAM variant and crRNA guide pUC ori vector
    pcDNA3.1-hLbCpf1(TYCV) (pY230)Lachnospiraceae bacterium Cpf1 (RR variant) (Synthetic)CMV Zhang Engineered Cpf1 variants with altered PAM specificities. Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. Expresses humanized LbCpf1 variant that recognizes TYCV PAMs pcDNA3.1
    L40C-CRISPR.EFS.dTomato Heckl CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo. Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 10.3324/haematol.2017.164046. Lentiviral CRISPR-Cas9 delivery for sgRNA (hU6), dTomato coexpression, EFS Promoter driven SIN40C
    L40C-CRISPR.EFS.PACPuromycin Heckl CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo. Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 10.3324/haematol.2017.164046. Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven SIN40C
    pRGEN-CMV-CjCas9Cas9 derived from Campylobacter jejuni (Other) Kim In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500. Expression of CjCas9 in mammalian cells pcDNA3.1
    CAG-Cas9hCas9 (Other)CAG Otonkoski Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9. Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11. Expresses high levels of WT Cas9 for genome editing pCAG
    CAG-SaCas9-WPRESaCas9 (Other)CAG Otonkoski Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9. Stem Cell Res. 2017 Jul;22:16-19. doi: 10.1016/j.scr.2017.05.005. Epub 2017 May 17. Expresses high levels of WT SaCas9 for genome editing pCAG NEWENTRY
    DD-Cas9 with filler sequence and Venus (EDCPV)Cas9 (Synthetic)EFS Sordella Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization. Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370. This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA lentiCRISPR v2
    DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)Cas9 (Synthetic)EFS Sordella Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization. Nat Commun. 2017 Feb 22;8:14370. doi: 10.1038/ncomms14370. This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA lentiCRISPR v2
    pSpCas9(BB)-2A-miRFP670hSpCas9-2A-miRFP670 (Synthetic), BB-guide RNA (Synthetic)Cbh, U6 Kuehn pSpCas9(BB)-2A-miRFP670 (unpublished) Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988 PX459
    LcV2-HygroHygromycin Mendell An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017 Feb 9;542(7640):197-202. doi: 10.1038/nature21025. Epub 2017 Jan 23. LentiCRISPR v2 with Hygro cloned in place of Puro LentiCRISPR v2
    pX330-Flag-SpCas9-HF1 (without sgRNA)3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS (Other)Cbh Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence) pX330-like (without U6-sgRNA coding sequence)
    HeFm1SpCas93xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS (Other)Cbh Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence) pX330-like (without U6-sgRNA coding sequence)
    HeFm2SpCas9“Highly enhanced Fidelity” mut2 SpCas9 (Other)Cbh Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence) pX330-like (without U6-sgRNA coding sequence)
    pY111 (pcDNA3.1-huTsCpf1)huTsCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized TsCpf1 pcDNA3.1
    pY112 (pcDNA3.1-huSaCpf1)huSaCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized SaCpf1 pcDNA3.1
    pY113 (pcDNA3.1-huPb2Cpf1)huPb2Cpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized Pb2Cpf1 pcDNA3.1
    pY114 (pcDNA3.1-huPgCpf1)huPgCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized PgCpf1 pcDNA3.1
    pY115 (pcDNA3.1-huMlCpf1)huMlCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized MlCpf1 pcDNA3.1
    pY116 (pcDNA3.1-huMb2Cpf1)huMb2Cpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized Mb2Cpf1 pcDNA3.1
    pY117 (pcDNA3.1-huMb3Cpf1)huMb3Cpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized Mb3Cpf1 pcDNA3.1
    pY118 (pcDNA3.1-huLb4Cpf1)huLb4Cpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized Lb4Cpf1 pcDNA3.1
    pY119 (pcDNA3.1-huLb5Cpf1)huLb5Cpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized Lb5Cpf1 pcDNA3.1
    pY120 (pcDNA3.1-huFbCpf1)huFbCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized FbCpf1 pcDNA3.1
    pY122 (pcDNA3.1-huCPbCpf1)huCPbCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized CPbCpf1 pcDNA3.1
    pY123 (pcDNA3.1-huCMaCpf1)huCMaCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized CMaCpf1 pcDNA3.1
    pY124 (pcDNA3.1-huBsCpf1)huBsCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized BsCpf1 pcDNA3.1
    pY125 (pcDNA3.1-huBfCpf1)huBfCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized BfCpf1 pcDNA3.1
    pY126 (pcDNA3.1-huBoCpf1)huBoCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized BoCpf1 pcDNA3.1
    pY127 (pcDNA3.1-U6-huTsCpf1)huTsCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized TsCpf1 and crRNA pcDNA3.1
    pY128 (pcDNA3.1-U6-huMb2Cpf1)huMb2Cpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized Mb2Cpf1 and crRNA pcDNA3.1
    pY129 (pcDNA3.1-U6-huMb3Cpf1)huMb3Cpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized Mb3Cpf1 and crRNA pcDNA3.1
    pY130 (pcDNA3.1-U6-huBsCpf1)huBsCpf1 (Other) Zhang Zhang lab Cpf1 plasmids (unpublished) Expression of humanized BsCpf1 and crRNA pcDNA3.1
    pX330-Flag-wtSpCas9 (without sgRNA)3xFlag-NLS-wild-type SpCas9-NLS (Other)Cbh Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence) pX330-like (without U6-sgRNA coding sequence)
    pX330-Flag-eSpCas9 (without sgRNA)3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS (Other)Cbh Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence) pX330-like (without U6-sgRNA coding sequence)
    HeFSpCas93xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS (Other)Cbh Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence) pX330-like (without U6-sgRNA coding sequence)
    pCAG Cas9-2A-CitrineCas9 2A Citrine (Other) Sauka-Spengler Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos. pCAG
    pTK Cas9-2A-CitrineCas9 2A Citrine (Other)thymidine kinase promoter Sauka-Spengler Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers. pTK
    pCI Cas9 H2B-RFPCas9 (Other)CAG Sauka-Spengler Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. CAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRES pCI IRES H2B RFP
    pXPR_206Puromycin Root Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens. Nat Biotechnol. 2018 Feb;36(2):179-189. doi: 10.1038/nbt.4048. Epub 2017 Dec 18. for SaCas9 knockout, lentiviral expression of SaCas9 and gRNA scaffold pXPR
    pPapiPuromycin ; (located downstream of SaCas9-2A sequence) Root Orthologous CRISPR-Cas9 enzymes for combinatorial genetic screens. Nat Biotechnol. 2018 Feb;36(2):179-189. doi: 10.1038/nbt.4048. Epub 2017 Dec 18. lentiviral expression of SaCas9 and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in Stable SpCas9 expressing cell lines for dual Cas9 "Big Papi" screens. Alt name pXPR207. pXPR
    pLX_311-Cas9Cas9 (Other)EF1aBlasticidin Hahn Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111 pXPR
    AAV_Efs_hSpCas9_NLS_FLAG-SV40humanized S. pyogenes Cas9 (Synthetic)EFS Yang Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76. AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter pAAV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)
  • Lenti_Efs_hSpCas9_NLS_FLAG-WPREhumanized S. pyogenes Cas9 (Synthetic)EFS Yang Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017 May 19. doi: 10.1038/cr.2017.76. Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter. Lenti virus
  • Tag / Fusion Protein
    • Flag (C terminal on insert)
  • lentiCRISPRv2 puroP2A-puroEF-1aPuromycin Stringer A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. Variant of lentiCRISPRv2 that confers puromycin resistance lentiCRISPRv2
    lentiCRISPRv2 hygroP2A-hygroEF-1aHygromycin Stringer A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. Variant of lentiCRISPRv2 that confers hygromycin resistance lentiCRISPRv2
    lentiCRISPRv2 neoP2a-neoEF-1aNeomycin (select with G418) Stringer A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. Variant of lentiCRISPRv2 that confers G418 resistance lentiCRISPRv2
    lentiCRISPRv2 blastP2a-blastEF-1aBlasticidin Stringer A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. Variant of lentiCRISPRv2 that confers blasticidin S resistance lentiCRISPRv2
    px330-mcherryU6mCherry Li Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cell Stem Cell. 2013 Dec 5;13(6):659-62. doi: 10.1016/j.stem.2013.10.016. Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA pX330
    pCAGG>nls-hCas9-nlshumanized Cas9Chicken beta actin (CAG) Bronner Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo. Dev Biol. 2017 Dec 1;432(1):86-97. doi: 10.1016/j.ydbio.2017.08.036. Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos pCI
    pCAGG>nls-hCas9-nls-GFPCas9-GFPChicken beta actin (CAG) Bronner Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo. Dev Biol. 2017 Dec 1;432(1):86-97. doi: 10.1016/j.ydbio.2017.08.036. Cas9-GFP fusion protein under the regulation of chicken beta-actin promoter pCI
    LentiCRISPRv2-mCherryCas9 (Synthetic), mCherry (Other)EFS-NS, EFS-NSmCherry Smogorzewska LentiCRISPRv2-mCherry (unpublished) Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry. LentiCRISPRv2
    CROPseq-Guide-EFS-SpCas9-P2A-EGFPEFS Hewitt CROPseq-Guide-EFS-SpCas9-P2A-EGFP (unpublished) single-vector system with EGFP reporter for use in CROPseq knockout screens CROPseq-Guide-Puro
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)
  • ciCas9_pcDNA5ciCas9Hygromycin Maly Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics. Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368. Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables. pcDNA5/FRT/TO
    e-ciCas9_pcDNA5e-ciCas9Hygromycin Maly Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics. Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368. Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables. pcDNA5/FRT/TO
    eSpCas9(1.1)-T2A-PuroCBhPuromycin Németh eSpCas9(1.1)-T2A-Puro (unpublished) Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker. eSpCas9(1.1)
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • T2A-Puro (C terminal on insert)
  • BPK3258 - human expression plasmid for eSpCas9(1.1)hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG JDS246
    BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG JDS246
    BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG JDS246
    MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926AhSpCas9-Cluster1+Q926A(N692A/M694A/Q695A/H698A/Q926A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG JDS246
    MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926AhSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG JDS246
    MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926AhSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG JDS246
    MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926AhSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG JDS246
    MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926AhSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG JDS246
    MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926AhSpCas9-Cluster2+Q926A(G582A/V583A/E584A/D585A/N588A/Q926A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG JDS246
    MMW2993 - human expression plasmid for SpCas9 Cluster 2hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG JDS246
    MMW3645 - human expression plasmid for SpCas9 Cluster 3hSpCas9-Cluster3(T657A/G658A/W659A/R661A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG JDS246
    MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926AhSpCas9-Cluster3+Q926A(T657A/G658A/W659A/R661A/Q926A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG JDS246
    MMW3770 - human expression plasmid for SpCas9 Cluster 4hSpCas9-Cluster4(F491A/M495A/T496A/N497A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG JDS246
    MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926AhSpCas9-Cluster4+Q926A(F491A/M495A/T496A/N497A/Q926A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG JDS246
    BPK4387 - human expression plasmid for SpCas9 Cluster 5hSpCas9-Cluster5(K918A/V922A/R925A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG JDS246
    BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926AhSpCas9-Cluster5+Q926A(K918A/V922A/R925A/Q926A) (Synthetic)CMV Joung Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature. 2017 Sep 20. doi: 10.1038/nature24268. Human expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG JDS246
    px459 VQRSpCas9 VQR (Other)Puromycin Holland AID Chapter plasmids (unpublished) sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM) px459 v2
    px459 VRERSpCas9 VRER (Other)Puromycin Holland AID Chapter plasmids (unpublished) sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM) px459 v2
    p458 VQRSpCas9 VQR (Other)EGFP Holland px458 VQR (unpublished) Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs px458
    p458 VRERSpCas9 VRER (Other)EGFP Holland px458 VQR (unpublished) Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs px458
    px330 VRERSpCas9 VRER (Other) Holland px458 VQR (unpublished) Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs px330
    p330 VQRSpCas9 VQR (Other) Holland px458 VQR (unpublished) Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs px330
    px458 EQRSpCas9 EQR (Other)EGFP Holland px458 VQR (unpublished) Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs px458
    px459 EQRSpCas9 EQR (Other)Puromycin Holland px458 VQR (unpublished) Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs px459 v2
    px330 EQRSpCas9 EQR (Other) Holland px458 VQR (unpublished) Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs px330
    pLCRISPR-CMV Manel Transmission of innate immune signaling by packaging of cGAMP in viral particles. Science. 2015 Sep 11;349(6253):1232-6. doi: 10.1126/science.aab3628. Epub 2015 Jul 30. Control lentivector expressing Cas9 and gRNA scaffold pLCRISPR
    PCS2+Cas9-mSACAS9-MSA (Other)SP6 Rossant Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos PCS2+
    p3s-Cas9-HNCas9-WT (Other)CMV Kim Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides. Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. Cas9-WT mammalian expression p3s
  • Tag / Fusion Protein
    • HA (N terminal on backbone)
  • p3s-eCas9-1.1eCas9-1.1 (Other)CMV Kim Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides. Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. eCas9-1.1 mammalian expression p3s
  • Tag / Fusion Protein
    • HA (N terminal on backbone)
  • p3s-Cas9-HF1Cas9-HF1 (Other)CMV Kim Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides. Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. Cas9-HF1 mammalian expression p3s
  • Tag / Fusion Protein
    • HA (N terminal on backbone)
  • pAAV-EFS-SpCas9Streptococcus pyogenes Cas9 (Synthetic)EFS Yasuda Virus-Mediated Genome Editing via Homology-Directed Repair in Mitotic and Postmitotic Cells in Mammalian Brain. Neuron. 2017 Oct 18. pii: S0896-6273(17)30933-9. doi: 10.1016/j.neuron.2017.10.004. AAV vector expressing Myc-tagged SpCas9 under the EFS promoter PX551
    lenti-SpCas9 puroKpnI-XhoI-BsrGI-NheI (Synthetic)Puromycin Stringer A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance. lentiCRISPRv2 puro (Addgene #98290)
    lenti-SpCas9 hygroKpnI-XhoI-BsrGI-NheI (Synthetic)Hygromycin Stringer A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. This lentiviral construct delivers hSpCas9 and hygromycin resistance. lentiCRISPRv2 hygro (Addgene #98291)
    lenti-SpCas9 neoKpnI-XhoI-BsrGI-NheI (Synthetic)Neomycin (select with G418) Stringer A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. This lentiviral construct delivers hSpCas9 and G418 resistance. lentiCRISPRv2 neo (Addgene #98292)
    lenti-SpCas9 blastKpnI-XhoI-BsrGI-NheI (Synthetic)Blasticidin Stringer A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma. Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z. This lentiviral construct delivers hSpCas9 and blasticidin S resistance. lentiCRISPRv2 blast (Addgene #98293)
    pMB-SaCas9Cas9 nuclease (Other)short EF1alphaHygromycin ; mouse CD4 McManus Dual gene activation and knockout screen reveals directional dependencies in genetic networks. Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062. SaCas9 expression vector pBR322
    pSECBBlasticidin Possemato Serine Catabolism by SHMT2 Is Required for Proper Mitochondrial Translation Initiation and Maintenance of Formylmethionyl-tRNAs Mol. Cell (2018) Volume 69, Issue 4, p610–621.e5 Express spCas9 with sgRNA, blast resistance pSECB
    AAV-CMVc-Cas9Cas9 (Synthetic)CMVc Belmonte In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7. Expresses SpCas9 from the CMVc promoter in AAV backbone AAV
    CROPseq-EFS-SpCas9-P2A-blastEFSBlasticidin Hewitt CROPseq plasmids (unpublished) single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens CROPseq-Guide-EFS-SpCas9-P2A-EGFP
    CROPseq-EFS-HypaSpCas9-P2A-EGFPHypaSpCas9 (Synthetic)EFSEGFP Hewitt CROPseq plasmids (unpublished) single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen CROPseq-Guide-EFS-SpCas9-P2A-EGFP
    CROPseq-EFS-HypaSpCas9-P2A-puroHypaSpCas9 (Synthetic)EFSPuromycin Hewitt CROPseq plasmids (unpublished) single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen CROPseq-Guide-EFS-SpCas9-P2A-puro
    pX551-CMV-SpCas9SpCas9 (Other) Hewitt Alex Hewitt Lab plasmids (unpublished) AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab pAAV
    pX551-miniCMV-SpCas9SpCas9 (Other) Hewitt Alex Hewitt Lab plasmids (unpublished) AAV plasmid expressing Cas9 under control of miniCMV promoter pAAV
    pX551-miniCMV-SaCas9SaCas9 (Other) Hewitt Alex Hewitt Lab plasmids (unpublished) AAV plasmid expressing SaCas9 under control of miniCMV promoter pAAV
    pX551-miniCMV-CjCas9CjCas9 (Other) Hewitt Alex Hewitt Lab plasmids (unpublished) AAV plasmid expressing CjCas9 under control of miniCMV promoter pAAV
    pX551-miniCMV-AsCpf1AsCpf1 (Other) Hewitt Alex Hewitt Lab plasmids (unpublished) AAV plasmid expressing AsCpf1 under control of miniCMV promoter pAAV
    pX551-CMV-CjCas9CjCas9 (Other) Hewitt Alex Hewitt Lab plasmids (unpublished) AAV plasmid expressing CjCas9 under control of CMV promoter pAAV
    pX601 miniCMV-SaCas9-SpA-sgRNA scaffold Hewitt Alex Hewitt Lab plasmids (unpublished) Backbone vector for cloning in target sgRNA for use with SaCas9 pAAV
    pSP64T-hLbCpf1hLbCpf1 (Homo sapiens)sp6 Giraldez CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2. plasmid to carry out IVT of human codon optimized LbCpf1 (from Addgene; 69988) pSP64T
    pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTAhSpCas9 (Other), rtTA, bGH polyA-SV40polyATRE, CAGPuromycin Menéndez Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locus. (unpublished) Expresses Cas9 upon induction with doxicycline. N/A
    pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLSSaCas9 (Other)CMV IE94 Wolfe Orthogonal Cas9-Cas9 chimeras provide a versatile platform for genome editing. Nat Commun. 2018 Nov 19;9(1):4856. doi: 10.1038/s41467-018-07310-x. Expresses SaCas9 in mammalian cells pCSDest2
    pTE3320hLbCpf1(RVR)CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells. pcDNA3.1
    pTE3314hFnCpf1(RVR)CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells. pcDNA3.1
    pTE3317hMbCpf1(RVR)CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells. pcDNA3.1
    pTE3316hFnCpf1(RR)CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells. pcDNA3.1
    pTE3319hMbCpf1(RR)CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells. pcDNA3.1
    pTE3330Lb crRNA, hLbCpf1(RVR mutant)human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells. pcDNA3.1
    pTE3327As crRNA, hAsCpf1(RVR mutant)human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells. pcDNA3.1
    pTE3331Fn crRNA, hFnCpf1(RVR mutant)human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells. pcDNA3.1
    pTE3334Mb crRNA, hMbCpf1(RVR mutant)human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells. pcDNA3.1
    pTE3329Lb crRNA, hLbCpf1(RR mutant)human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells. pcDNA3.1
    pTE3328As crRNA, hAsCpf1(RR mutant)human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells. pcDNA3.1
    pTE3333Fn crRNA, hFnCpf1(RR mutant)human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells. pcDNA3.1
    pTE3336Mb crRNA, hMbCpf1(RR mutant)human U6, CMVNeomycin (select with G418) Welker Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants. Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815. Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells. pcDNA3.1
    pX-evoCas9Humanized S. pyogenes Cas9CBh Cereseto A highly specific SpCas9 variant is identified by in vivo screening in yeast. Nat Biotechnol. 2018 Jan 29. pii: nbt.4066. doi: 10.1038/nbt.4066. Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells PX330 (Addgene #42230)
    LentiV_Cas9_puroCas9 (Synthetic)EFS promoter, EFS promoterPuromycin Vakoc LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia. Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011. Lentiviral expression plasmid of spCas9 with puromycin resistance gene LentiV_puro
    pX459V2.0-eSpCas9(1.1)Puromycin Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations pX459
    pX459V2.0-SpCas9-HF1Puromycin Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations pX459
    pX459V2.0-HypaCas9Puromycin Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations pX459
    pX459V2.0-SpCas9-HF4Puromycin Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations pX459
    pX459V2.0-eSpCas9(1.1)+K810APuromycin Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations pX459
    pX459V2.0-eSpCas9(1.1)+K855APuromycin Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations pX459
    pX459V2.0-eSpCas9(1.1)+K810A+K855APuromycin Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations pX459
    pX330-SpCas9-HF1 Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations pX330
    pX330-HypaCas9 Miyaoka Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair Nucleic Acids Research pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations pX330
    xCas9 3.7xCas9 3.7 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9 3.7 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9 3.6xCas9 3.6 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9 3.6 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • Myh6-Cas9Cas9 (Other)alpha MHC van Rooij Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption. Circ Res. 2017 Oct 27;121(10):1168-1181. doi: 10.1161/CIRCRESAHA.116.310370. Epub 2017 Aug 29. Cardiac-specific overexpression of Cas9 alpha MHC
    1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA Lagor A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing. Mol Ther Methods Clin Dev. 2018 Dec 6;12:111-122. doi: 10.1016/j.omtm.2018.11.009. eCollection 2019 Mar 15. Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA pEMBL8
    1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA Lagor Somatic Editing of Ldlr With Adeno-Associated Viral-CRISPR Is an Efficient Tool for Atherosclerosis Research. Arterioscler Thromb Vasc Biol. 2018 Jul 19. pii: ATVBAHA.118.311221. doi: 10.1161/ATVBAHA.118.311221. Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA pEMBL8
    hSyn1-AsCpf1(RR)hAsCpf1(RR) (Homo sapiens)hSyn1 Zhang Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons Nature Biomedical Engineering (Epub 09 April 2018) AAV vector expressing AsCpf1 (RR variant) Custom
    pMIA3EF1a Foo A Roadmap for Human Liver Differentiation from Pluripotent Stem Cells. Cell Rep. 2018 Feb 20;22(8):2190-2205. doi: 10.1016/j.celrep.2018.01.087. Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB px330 (addgene #42230)
  • Tags / Fusion Proteins
    • mRuby2 (N terminal on insert)
    • mtp53dn (N terminal on insert)
  • pCas9-HEHDR-Enhancer domain of human CtIP protein (Homo sapiens)Neomycin (select with G418) Concordet CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7. Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein hCas9 Addgene ID 41815 RBBP8 COM1, CTIP, JWDS, RIM, SAE2, SCKL2
    pCas9-GemininGeminin (Homo sapiens)Neomycin (select with G418) Concordet CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7. Expresses Cas9 fusion to Geminin degron domain hCas9 Addgene ID 41815 GMNN Gem, MGORS6
    pCas9-HE-GemininHE-GemininNeomycin (select with G418) Concordet CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7. Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron hCas9 Addgene ID 41815
    pCas9-CtIPCtIP (Homo sapiens)Neomycin (select with G418) Concordet CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Nat Commun. 2018 Mar 19;9(1):1133. doi: 10.1038/s41467-018-03475-7. Expresses Cas9 fusion to human CtIP protein hCas9 Addgene ID 41815 RBBP8 COM1, CTIP, JWDS, RIM, SAE2, SCKL2
    pX458_RubyhSpCas9 Hublitz Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling. Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483. Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA PX458
    pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNASt1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens)LP1B, hU6 Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA pX602 (Plasmid #61593)
    MSP1594_2x_NLSSt1Cas9 LMD-9 (Homo sapiens), None (Synthetic)CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) MSP1594 (Plasmid #65775)
    U6_sgRNA_CAG_hSt1Cas9_LMD9St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens)CAG, hU6 Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA. MSP1594_2x_NLS (Plasmid #110625)
    St1Cas9_LMD-9_sgRNA_pUC19Empty St1Cas9 LMD-9 sgRNA backbone (Homo sapiens)hU6 Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9). pUC19
    pLenti-Cas9-P2A-PuroCas9 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-VQR-P2A-PuroCas9-VQR (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-VRER-P2A-PuroCas9-VRER (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-HF1-P2A-PuroCas9-HF1 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-VQRRA-P2A-PuroCas9-VQRRA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-VRERRA-P2A-PuroCas9-VRERRA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-HF1RA-P2A-PuroCas9-HF1RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-VQR-PGK-PuroCas9-VQR (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VQR (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-VRER-PGK-PuroCas9-VRER (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VRER (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-HF1-PGK-PuroCas9-HF1 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-VQRRA-PGK-PuroCas9-VQRRA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-VRERRA-PGK-PuroCas9-VRERRA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VRERRA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-HF1RA-PGK-PuroCas9-HF1RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-VQR-P2A-GFP-PGK-PuroCas9-VQR (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-VRER-P2A-GFP-PGK-PuroCas9-VRER (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-HF1-P2A-GFP-PGK-PuroCas9-HF1 (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)
  • pLenti-VQRRA-P2A-GFP-PGK-PuroCas9-VQRRA (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-VRERRA-P2A-GFP-PGK-PuroCas9-VRERRA (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-HF1RA-P2A-GFP-PGK-PuroCas9-HF1RA (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pLenti-xCas9RA-P2A-PuroxCas9(3.7)RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)
  • pEJS654 All-in-One AAV-sgRNA-hNmeCas9sgRNA scaffold, Human-codon-optimized NmeCas9 (Other)U6, U1a Sontheimer All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo. Genome Biol. 2018 Sep 19;19(1):137. doi: 10.1186/s13059-018-1515-0. Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing. PX551-Plasmid #60957
    pX458-DsRed2DsRed2 (Synthetic) Liang Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion. J Biotechnol. 2018 Jun 7;281:11-20. doi: 10.1016/j.jbiotec.2018.06.308. Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2 pX458
    pX458-ECFPECFP (Synthetic) Liang Speed genome editing by transient CRISPR/Cas9 targeting and large DNA fragment deletion. J Biotechnol. 2018 Jun 7;281:11-20. doi: 10.1016/j.jbiotec.2018.06.308. Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP pSpCas9(BB)-2A-GFP (PX458)
    CMV-SpyCas9SpyCas9 Niopek Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. SpyCas9 expression in mammalian cells (CMV promoter driven) pcDNA3.1
    pAAV-CMV-SpyCas9SpyCas9 Niopek Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. AAV vector for expression of SpyCas9 in mammalian cells ssAAV vector
    pX330 spCas9-mSACAS9-MSA (Other) Rossant Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines pX330
    pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pASaCas9Cytomegalo Virus(CMV) Ploski The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Front. Mol. Neurosci. (2018) 13 SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS. AAV NEWENTRY
    pJEP313-pAAV-CMV-SaCas9-DIO-pASaCas9Cytomegalo Virus(CMV) Ploski The Development of an AAV-Based CRISPR SaCas9 Genome Editing System That Can Be Delivered to Neurons in vivo and Regulated via Doxycycline and Cre-Recombinase. Front. Mol. Neurosci. (2018) 13 A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent. AAV NEWENTRY
    p3s-Sniper-Cas9Sniper-Cas9CMV Lee Directed evolution of CRISPR-Cas9 to increase its specificity. Nat Commun. 2018 Aug 6;9(1):3048. doi: 10.1038/s41467-018-05477-x. Expresses Sniper-Cas9 with CMV promoter pCDNA3.1
    pSpCTRE-CD4SpCas9 (Homo sapiens)CD4 Rudin Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics Nature Cancer 1, 359–369 (2020) Dox-inducible Cas9 expression with a CD4 selection marker pLVX-TetOne-Puro
    pSH231-EFS-Cas9-BlastRCas9-T2A-BlastR (Other)EF1aBlasticidin Monnat New human chromosomal safe harbor sites for genome engineering with CRISPR/Cas9, TAL effector and homing endonucleases bioRxiv 396390 Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette Piggybac
    pU6-SacB-Cas9-T2A-mCherry_(BbsI)Cas9 (Other), SacB (Other)CBh, U6T2A-mCherry Failor CRISPR-Cas9 system with SacB toxin Positive Selection (unpublished) Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection pX330
    pBC2101-recireci_wtCas9 with SV40 NLS Savage Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS Lenti
    pBC2102-recireci_wtCas9 without NLSU6 Promoter Savage Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS Lenti
    pBC2103-recireci_arCas9 without NLS Savage Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528. U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS Lenti
    eSpCas9(1.1)-PuroeSpCas9(1.1) (Synthetic)U6Puromycin Leyns Loss of Emp2 compromises cardiogenic differentiation in mouse embryonic stem cells. Biochem Biophys Res Commun. 2019 Feb 14. pii: S0006-291X(19)30235-9. doi: 10.1016/j.bbrc.2019.02.048. The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein. pSpCas9(BB)-2A-Puro
    pScCas9Streptococcus canis Cas9 (Other)EF-1α Jacobson Minimal PAM specificity of a highly similar SpCas9 ortholog. Sci Adv. 2018 Oct 24;4(10):eaau0766. doi: 10.1126/sciadv.aau0766. eCollection 2018 Oct. Mammalian expression vector encoding WT ScCas9 OG3569
    pX330-SpCas9-NGSpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) Nureki Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129. Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells. pX330
    pLex_Cas9Cas9-P2A-NLS-BASTR (Other)Blasticidin Khavari Coupled Single-Cell CRISPR Screening and Epigenomic Profiling Reveals Causal Gene Regulatory Networks. Cell. 2018 Dec 11. pii: S0092-8674(18)31514-9. doi: 10.1016/j.cell.2018.11.022. pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter. pLex
    pLX311-Cas9Cas9 (Other)EF1alphaBlasticidin Hahn Mutational processes shape the landscape of TP53 mutations in human cancer. Nat Genet. 2018 Oct;50(10):1381-1387. doi: 10.1038/s41588-018-0204-y. Constitutive expression of Cas9 pLX311
    pSpCas9(BB)-2A-BlastBlasticidin Takemaru pSpCas9(BB)-2A-Blast (unpublished) Used for expression of sgRNA in mammalian cells pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988)
    pSpCas9(BB)-T2A-HygRhSpCas9-T2A-HygR (Homo sapiens)CAG and U6Hygromycin Ferrer Human pancreatic islet three-dimensional chromatin architecture provides insights into the genetics of type 2 diabetes. Nat Genet. 2019 Jun 28. pii: 10.1038/s41588-019-0457-0. doi: 10.1038/s41588-019-0457-0. SpCas9 expression vector pX458
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on insert)
  • pCAG-SpCas9-2A-GFP-noITRCas9 (Other) Denham A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity. Front Cell Dev Biol. 2018 May 15;6:54. doi: 10.3389/fcell.2018.00054. eCollection 2018. Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression pSpCas9(BB)-2A-GFP (PX458)
    HF-PX459 (V2)hSpCas9-HF1-2A-Puro V2.0 (Synthetic)CbhPuromycin McGrew High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells. Sci Rep. 2018 Oct 11;8(1):15126. doi: 10.1038/s41598-018-33244-x. Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance PX459
    Lenti_SaCRISPR_GFPU6GFP Vakoc A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence. Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29. To clone sgRNA for SaCas9 Lenti_SaCRISPR_GFP
    Nme2Cas9_pCDestNme2Cas9 (Other)CMV Sontheimer A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing. Mol Cell. 2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 10.1016/j.molcel.2018.12.003. Nme2Cas9 CMV-driven mammalian expression plasmid pCDest
    Nme2Cas9_AAVNme2Cas9 (Other)U1a Sontheimer A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing. Mol Cell. 2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 10.1016/j.molcel.2018.12.003. All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette AAV
    AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)N-terminal fragment of SpyCas9 (Other) Niopek Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271. AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold pZac2.1
  • Tags / Fusion Proteins
    • SV40-NLS (N terminal on insert)
    • split-intein (C terminal on insert)
  • EF1a-Cas9-2XNES-2ERT2Cas9, NES and ERT2 (Synthetic)EF1αZeocin Wang HIT-Cas9: A CRISPR/Cas9 Genome-Editing Device under Tight and Effective Drug Control. Mol Ther Nucleic Acids. 2018 Dec 7;13:208-219. doi: 10.1016/j.omtn.2018.08.022. Epub 2018 Sep 1. Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells pRRL.sin-18.ppy.
    pAAV.pU1a-SpCas9SpCas9 (Synthetic)U1a Gao Cas9-mediated allelic exchange repairs compound heterozygous recessive mutations in mice. Nat Biotechnol. 2018 Oct;36(9):839-842. doi: 10.1038/nbt.4219. Epub 2018 Aug 13. Expresses codon-optimized SpCas9 in mammalian cells. pAAV
    pCF204Cas9 (Other)U6Puromycin Savage CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052. U6-sgRNA EFS-Cas9-wt-P2A-Puro pU6
    pCF226cas9 (Other)EF-1a core promoterPuromycin Savage CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052. EFS-Cas9-wt-P2A-Puro pEFS
    pLenti spCas9 T2A iRFP670 P2A purospCas9, iRFP670Puromycin Gaudin Lenti spCas9 T2A iRFP670 P2A puro (unpublished) The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences. pLenti NEWENTRY
    pLenti spCas9 T2A mNeonGreen P2A purospCas9, mNeonGreenPuromycin Gaudin Lenti spCas9 T2A iRFP670 P2A puro (unpublished) The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences. pLenti NEWENTRY
    pLenti spCas9 T2A TagRFP-T P2A purospCas9, TagRFP-T Gaudin Lenti spCas9 T2A iRFP670 P2A puro (unpublished) The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences. pLenti
    pSaCas9-1xms2-2x3’UTRSaCas9-MS2-HBB 3' UTR (Other) Lu Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093. For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
    pSaCas9-1xPP7-2x3'UTRSaCas9-PP7-HBB 3' UTR (Other) Lu Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093. For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
    PCS2+ Cas9Cas9 (Synthetic)SP6 Rossant Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos PCS2+
    pRC10-EFS-huLbCpf1-2A-Blast-WPREhuman codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium, (Synthetic)EFS-NSBlasticidin Chen In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens. Nat Methods. 2019 May;16(5):405-408. doi: 10.1038/s41592-019-0371-5. Epub 2019 Apr 8. Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection. pFUGW
    KA2058_pX330A-G2PPuromycin Schöler Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. Expression of SpCas9 and sgRNA pX330
    KA2601_eSpCas9-G2PPuromycin Schöler Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. Expression of eSpCas9(1.1) and sgRNA eSpCas9(1.1)
    KA2706_eSpCas9-PGKPacdtkChPuromycin Schöler Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. Expression of eSpCas9(1.1) and sgRNA eSpCas9(1.1)
    KA2963_eSpCas9-PGKHygdtkChHygromycin Schöler Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency. Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14. Expression of eSpCas9(1.1) and sgRNA eSpCas9(1.1)
    pX330-NL-DHFR-SpCas9NL-DHFR-SpCas9 (Other)Cbh Choudhary A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR-Cas9. Angew Chem Int Ed Engl. 2019 May 6;58(19):6285-6289. doi: 10.1002/anie.201900788. Epub 2019 Apr 2. Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells pX330
    pX330-NLC-DHFR-SpCas9NLC-DHFR-SpCas9 (Other)Cbh Choudhary A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR-Cas9. Angew Chem Int Ed Engl. 2019 May 6;58(19):6285-6289. doi: 10.1002/anie.201900788. Epub 2019 Apr 2. Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells pX330
    Lenti-Cas9-gRNA-GFPGFP Sheltzer Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9. Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100. 3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone LRG2.1 (Addgene #108098)
    Lenti-Cas9-gRNA-TagBFP2TagBFP2 Sheltzer Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9. Curr Protoc Mol Biol. 2019 Sep;128(1):e100. doi: 10.1002/cpmb.100. 3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone Lenti-Cas9-gRNA-GFP (Addgene #124770)
    pAAV-FLEX-SaCas9-U6-sgRNAgRNA scaffold (Mus musculus) Zweifel Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System. Cell Rep. 2020 Mar 24;30(12):4303-4316.e6. doi: 10.1016/j.celrep.2020.02.092. Vector for Cre-dependent expression of SaCas9 pX601
    pMSCV-Cas9-2A-GFP-sgRNACas9 (Synthetic)MSCV Lodish Efficient CRISPR-Cas9 mediated gene disruption in primary erythroid progenitor cells. Haematologica. 2016 Jun;101(6):e216-9. doi: 10.3324/haematol.2015.135723. Epub 2016 Mar 11. Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector pMCSV
    pAAVS1-tet-iCas9-BFP2AAVS1-TET-ON 3G-SpCas9-P2A-BFP2 (Homo sapiens)U6Neomycin (select with G418) Moffat Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Cell Rep. 2019 Apr 9;27(2):599-615.e12. doi: 10.1016/j.celrep.2019.02.041. all-in-one-inducible Cas9 pAAVS1
    LentiV_Cas9_BlastCas9 (Synthetic)EFS promoter, EFS promoterBlasticidin Vakoc Salt-Inducible Kinase inhibition suppresses acute myeloid leukemia progression in vivo. Blood. 2019 Nov 1. pii: 422697. doi: 10.1182/blood.2019001576. Lentiviral expression of spCas9 with blasticidin resistance gene LentiV_Blast
    PB_tre_Cas9humanized S. pyrogenes Cas9, Hygromycin resistanceTRE, EF1 alphaHygromycin Calabrese A piggyBac-based toolkit for inducible genome editing in mammalian cells. RNA. 2019 May 17. pii: rna.068932.118. doi: 10.1261/rna.068932.118. PiggyBac cargo vector expressing doxycycline inducible Cas9 piggyBac cargo vector
    pcDNA-Cas9-T2A-STOP-TdTCas9-T2A-STOP-TdT (Homo sapiens)pCMVHygromycin Liu DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cells bioRxiv (2019) Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552) pcDNA3.1
    pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)WT SpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations) pX330-like (without U6-sgRNA coding sequence)
    pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)eSpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations) pX330-like (without U6-sgRNA coding sequence)
    pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)SpCas9-HF1 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations) pX330-like (without U6-sgRNA coding sequence)
    pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)HypaSpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations) pX330-like (without U6-sgRNA coding sequence)
    pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)evoSpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations) pX330-like (without U6-sgRNA coding sequence)
    pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)HeFSpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations) pX330-like (without U6-sgRNA coding sequence)
    B-SpCas9B-SpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9. pX330-like (without U6-sgRNA coding sequence)
    B-eSpCas9B-eSpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9. pX330-like (without U6-sgRNA coding sequence)
    B-SpCas9-HF1B-SpCas9-HF1 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1. pX330-like (without U6-sgRNA coding sequence)
    B-HypaSpCas9B-HypaSpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9. pX330-like (without U6-sgRNA coding sequence)
    B-evoSpCas9B-evoSpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9. pX330-like (without U6-sgRNA coding sequence)
    B-HeFSpCas9B-HeFSpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9. pX330-like (without U6-sgRNA coding sequence)
    eSpCas9-pluseSpCas9-plus (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9. pX330-like (without U6-sgRNA coding sequence)
    SpCas9-HF1-plusSpCas9-HF1-plus (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1 pX330-like (without U6-sgRNA coding sequence)
    pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)Sniper SpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations) pX330-like (without U6-sgRNA coding sequence)
    pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)HiFi SpCas9 (Other)CBh Welker Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities Nature Communications, 1223, 2020 Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations) pX330-like (without U6-sgRNA coding sequence)
    TLCV2-LoxPCas9-2A-eGFP (Homo sapiens)U6Puromycin Karpf Karpf Lab plasmids (unpublished) LentiCRISPR v2 was modified into an all-in-one dox inducible system. lentiCRISPR v2
    pFUGW Cas9-BFPCas9 (Other)SVVF Zhuang Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization. Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 10.1073/pnas.1903808116. Epub 2019 May 13. Cas9 expression pFUGW
    LentiAsCpf1-3xMYC-blastAsCpf1 (Synthetic)Blasticidin Draetta Pooled library screening with multiplexed Cpf1 library. Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x. Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal pFUGW
    pSpCas9(BB)-2A-NeoEmpty backboneNeomycin (select with G418) Takemaru Used for expression of sgRNA in mammalian cells (unpublished) Used for expression of sgRNA in mammalian cells pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988)
    pSpCas9(BB)-2A-HygroHygromycin Takemaru Used for expression of sgRNA in mammalian cells (unpublished) Used for expression of sgRNA in mammalian cells pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene#62988)
    pX330GFP-hU6-gRNA-hSpCas9GFP, gRNA and hSpCas9 Tucker CRISPR-Cas9-Based Genome Editing of Human Induced Pluripotent Stem Cells. (unpublished) GFP positive gRNA/hSpCas9 construct pX330
    pCDNA3.3 CAS9 IRES-DsredFnCas9 IRES-Dsred (Synthetic) Laur Emory Custom Cloning Core Plasmids - Oskar Laur (unpublished) expression of FnCas9 and DsRed pCDNA3.3 CAS9
    p1195-pssAAV.U1a.hNme2Cas9codon-optimized Nme2Cas9 (Other)U1a Sontheimer Tissue-restricted Genome Editing in vivo Specified by MicroRNA-repressible Anti-CRISPR Proteins. RNA. 2019 Aug 22. pii: rna.071704.119. doi: 10.1261/rna.071704.119. AAV vector expressing Nme2Cas9 AAV
    pCas9/VRQR-sgRNA (BbsI)Puromycin ; mCherry for FACS enrichment Ikonen An efficient auxin-inducible degron system with low basal degradation in human cells. Nat Methods. 2019 Aug 26. pii: 10.1038/s41592-019-0512-x. doi: 10.1038/s41592-019-0512-x. Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM pGL3-basic
    PX458-3xHA-SpCas93xHA-NLS-SpCas9 (Synthetic)Cbh Chakraborty Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing. Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 10.1073/pnas.1818461116. Epub 2019 Sep 30. 3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA PX458
    PX458-3xHA-FnCas93xHA-NLS-FnCas9 (Synthetic)Cbh Chakraborty Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing. Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 10.1073/pnas.1818461116. Epub 2019 Sep 30. 3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA PX458
    pX458M-53BP1-DN1SSpCas9-53BP1-DN1S (Homo sapiens)CBH Malik CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites. Nat Commun. 2019 Jun 28;10(1):2866. doi: 10.1038/s41467-019-10735-7. Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence. PX458M TP53BP1 53BP1, TDRD30, p202, p53BP1
    pSaCas9-sgRNA-Tetra-com vectorvector for com modified sgRNA (Synthetic) Lu Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing. Nucleic Acids Res. 2019 Jul 12. pii: 5531787. doi: 10.1093/nar/gkz605. For expressing gRNA, whose Tetraloop is replaced with a com aptamer pX601
    pCAG-T3-SpCAS9-NG-pACodon optimized SpCas9-NG (Synthetic) Fujii Generation of genetically modified mice using SpCas9-NG engineered nuclease. Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.1038/s41598-019-49394-5. Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys pCAGGS
    pCAG-T3-eSpCAS9-NG-pACodon optimized eSpCas9-NG (Synthetic) Fujii Generation of genetically modified mice using SpCas9-NG engineered nuclease. Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.1038/s41598-019-49394-5. Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys pCAGGS
    pCAG-T3-HypaCAS9-pACodon optimized HypaCas9 (Synthetic) Fujii High-fidelity endonuclease variant HypaCas9 facilitates accurate allele-specific gene modification in mouse zygotes. Commun Biol. 2019 Oct 10;2:371. doi: 10.1038/s42003-019-0627-8. eCollection 2019. Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys pCAGGS
    pORANGE Cloning template vectorSpCas9 (Other)U6 and Cbh MacGillavry ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. Cloning template form ORANGE method based knock-in constructs pORANGE
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • pFUGW SpCas9SpCas9 (Other)hUBC MacGillavry ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. lentiviral expression of SpCas9 under hUBC promotor pFUGW
    pCAG-T3-st1CAS9-pACodon optimized st1Cas9 (Synthetic) Fujii Zygote-mediated generation of genome-modified mice using Streptococcus thermophilus 1-derived CRISPR/Cas system. Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30988-3. doi: 10.1016/j.bbrc.2016.06.070. Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys pCAG-T3-hCAS9-pA
    pCAG-T3-CjCAS9-pACodon optimized CjCas9 (Synthetic)CAG, T3 Fujii Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas. Int J Mol Sci. 2017 Oct 31;18(11). pii: ijms18112286. doi: 10.3390/ijms18112286. Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys pCAG-T3-hCAS9-pA
    pAWp63-clone32 (Opti-SpCas9)Opti-SpCas9 (Synthetic)EFSZeocin Wong Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9. Nat Methods. 2019 Aug;16(8):722-730. doi: 10.1038/s41592-019-0473-0. Epub 2019 Jul 15. Expresses Opti-SpCas9 in mammalian cells pFUGW
    pAWp63-clone3-12 (OptiHF-SpCas9)OptiHF-SpCas9 (Synthetic)EFSZeocin Wong Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9. Nat Methods. 2019 Aug;16(8):722-730. doi: 10.1038/s41592-019-0473-0. Epub 2019 Jul 15. Expresses OptiHF-SpCas9 in mammalian cells pFUGW
    pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdTCas9-ODD-T2A-TdT (Homo sapiens)4xHRE_YB TATABlasticidin Liu DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cells bioRxiv (2019) Expresses hypoxia-inducible Cas9 and TdT in mammalian cells. pcDNA3.1
    SiC-V1SpCas9 (Synthetic) Neelamegham Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing. Mol Ther. 2019 Sep 12. pii: S1525-0016(19)30409-5. doi: 10.1016/j.ymthe.2019.09.006. SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter. LentiCRISPR v2
    BII-gR-PtW-eSpCas9eSpCas9 (Other)CMV enhancer and hEf1a (CpG free)Puromycin Madison Madison Lab - manuscript in preparation (unpublished) Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression BII-gR-PtW-EspCas9
  • Tag / Fusion Protein
    • HA-tagged eSpCas9 (N terminal on insert)
  • BII-gR-BtW-eSpCas9eSpCas9 (Other)CMV enhancer and hEf1a (CpG free)Blasticidin Madison Madison Lab - manuscript in preparation (unpublished) Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression BII-gR-BtW-EspCas9
  • Tag / Fusion Protein
    • Flag-tagged eSpCas9 (N terminal on insert)
  • BII-ChBtW-eSpCas9eSpCas9 (Other)CMV enhancer and hEf1a (CpG free)Blasticidin Madison Madison Lab - manuscript in preparation (unpublished) Piggybac vector for constitutive expression BII-ChBtW
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)
  • BII-ChPtW-eSpCas9eSpCas9 (Other)CMV enhancer and hEf1a (CpG free)Puromycin Madison Madison Lab - manuscript in preparation (unpublished) Piggybac vector for constitutive expression BII-ChPtW
  • Tag / Fusion Protein
    • HA (N terminal on insert)
  • pXPR_212control guidePuromycin Doench Genetic screens in isogenic mammalian cell lines without single cell cloning. Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6. U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance lentiCRISPRv2
    pXPR_124SpyoCas9 (Other) Doench Genetic screens in isogenic mammalian cell lines without single cell cloning. Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6. EF1a promoter expresses SpyoCas9 and P2A provides EGFP pLV
    pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)human codon optimized St3Cas9 (Synthetic)CAG Kleinstiver Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer. Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26. CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40) pCAG (Addgene #11179)
  • Tag / Fusion Protein
    • NLS(SV40)-3xFLAG (C terminal on backbone)
  • pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)human codon optimized CjeCas9 (Synthetic)CAG Kleinstiver Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer. Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26. CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40) pCAG (Addgene #11179)
  • Tag / Fusion Protein
    • NLS(SV40)-3xFLAG (C terminal on backbone)
  • pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)human codon optimized NmeCas9 (Synthetic)CAG Kleinstiver Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer. Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26. CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40) pCAG (Addgene #11179)
  • Tag / Fusion Protein
    • NLS(SV40)-3xFLAG (C terminal on backbone)
  • HP138-puroContains Cas9 (Other)Puromycin Cheeseman Cheeseman Lab Plasmids (unpublished) Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection. unknown
    HP138-neoContains Cas9 (Other)Neomycin (select with G418) Cheeseman Cheeseman Lab Plasmids (unpublished) Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection unknown
    pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)human codon optimized Nme2Cas9 (Synthetic)CAG Kleinstiver Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer. Nat Microbiol. 2020 Apr;5(4):620-629. doi: 10.1038/s41564-020-0692-2. Epub 2020 Mar 26. CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag pCAG (Addgene plasmid #11179)
  • Tag / Fusion Protein
    • NLS(SV40)-3xFLAG (C terminal on insert)
  • px458_Conc2U6GFP Stange Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoid J. Vis. Exp. 2020, e60704 Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP pSpCas9(BB)-2A-GFP (PX458)
    px458_Conc3U6GFP Stange Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoid J. Vis. Exp. 2020, e60704 Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP pSpCas9(BB)-2A-GFP (PX458)
    px459V2.0_Conc2U6Puromycin Stange Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoid J. Vis. Exp. 2020, e60704 Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro pSpCas9(BB)-2A-Puro (PX459) V2.0
    px459V2.0_Conc3U6Puromycin Stange Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoid J. Vis. Exp. 2020, e60704 Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + Puro pSpCas9(BB)-2A-Puro (PX459) V2.0
    pCAG-CFP-SaCas9-HF (without sgRNA)Staphylococcus aureus Cas9 nucleases with high genome-wide specificity Zheng Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity. Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 1906843116. doi: 10.1073/pnas.1906843116. Human expression vector for SaCas9-HF variant. pCAG-CFP
    pL40C_PGKintron_Cas9_GreenCas9-P2A-mNeonGreen (Other)hPGK promoter with beta-globin intron Bornhauser The Leukemogenic TCF3-HLF Complex Rewires Enhancers Driving Cellular Identity and Self-Renewal Conferring EP300 Vulnerability. Cancer Cell. 2019 Dec 9;36(6):630-644.e9. doi: 10.1016/j.ccell.2019.10.004. Epub 2019 Nov 14. Lentiviral vector coding Cas9 and mNeonGreen L40C
    pU6-(BsaI)_CBh-Cas9humanized S. pyogenes Cas9 (Other)CBh Shaul Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 pX330
    pU6-(BsaI)_CBh-UN-Cas9humanized S. pyogenes Cas9 (Other)CBh Shaul Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9 pX330
    pU6-(BsaI)_CBh-Cas9-T2A-mCherryhumanized S. pyogenes Cas9 (Other)CBh Shaul Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230)
    pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherryhumanized S. pyogenes Cas9 (Other)CBh Shaul Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230)
    pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherryhumanized S. pyogenes Cas9 (Other)CBh Shaul Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing. Biomolecules. 2019 Oct 8;9(10). pii: biom9100584. doi: 10.3390/biom9100584. Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene 64324), modification of pX330 (Addgene 42230)
    epiCRISPR Wang An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells. Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y. Expresses SpCas9, and cloning backbone for sgRNA epiCRISPR plasmid
    epiCRISPRe Wang An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells. Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y. Expresses SpCas9, and cloning backbone for sgRNA epiCRISPR plasmid
    pAAV-CMV-SauriCas9 Wang A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar. Expresses SauriCas9, and cloning backbone for sgRNA AAV2 ITR vector
    pAAV-CMV-SauriCas9-PuroPuromycin Wang A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar. Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA AAV2 ITR vector
    pAAV-CMV-SauriCas9-KKH-PuroPuromycin Wang A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar. Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA AAV2 ITR vector
    KO plasmid Wang krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells. J Biol Eng. 2019 Apr 24;13:35. doi: 10.1186/s13036-019-0150-y. eCollection 2019. Expresses SpCas9, and cloning backbone for sgRNA epiCRISPR plasmid
    U6_sgRNA_CAG_hSt1Cas9_CNRZ1066St1Cas9 LMD-9:CNRZ_1066 Chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens)CAG, hU6 Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:CNRZ1066 chimera) and its U6-driven sgRNA. MSP1594_2x_NLS (Plasmid #110625)
    U6_sgRNA_CAG_hSt1Cas9_LMG18311St1Cas9 LMD9:LMG18311 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens)CAG, hU6 Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA. MSP1594_2x_NLS (Plasmid #110625)
    U6_sgRNA_CAG_hSt1Cas9_TH1477St1Cas9 LMD-9:TH1477 Chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens)CAG, hU6 Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA. MSP1594_2x_NLS (Plasmid #110625)
    U6_sgRNA_CAG_hSt1Cas9_MTH17CL396St1Cas9 LMD-9:MTH17CL396 Chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens)CAG, hU6 Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA. MSP1594_2x_NLS (Plasmid #110625)
    pCMV-Cas9-NRRHCas9-NRRH (Other)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses Cas9-NRRH in mammalian cells pBR322 CMV
    pCMV-Cas9-NRTHCas9-NRTH (Other)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses Cas9-NRTH in mammalian cells pBR322 CMV
    pCMV-Cas9-NRCHCas9-NRCH (Other)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses Cas9-NRCH in mammalian cells pBR322 CMV
    pCF820_U6-sgRNA-EF1a-mCherry2_lentiSpyCas9 GFP sgRNAmCherry2 Doudna Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117. Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP pCF525
    pCF823_EFS-Cas9-PuroR_lentiSpyCas9Puromycin Doudna Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117. Lenti expression of SpyCas9 with Puro selection pCF525
    pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lentiSauCas9 GFP sgRNAmCherry2 Doudna Potent CRISPR-Cas9 inhibitors from Staphylococcus genomes. Proc Natl Acad Sci U S A. 2020 Mar 10. pii: 1917668117. doi: 10.1073/pnas.1917668117. Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP pCF525
    pHLS-EF1a-FRB-SpCas9-ASpCas9, human codon optimized (Other) Hotta Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping. Nat Commun. 2020 Mar 13;11(1):1334. doi: 10.1038/s41467-020-14957-y. Expresses FRB fused SpCas9 for NanoMEDIC packaging. pHLS
    eSpCas9(1.1)eSpCas9(1.1) (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD pFUGW
    SpCas9-HF1SpCas9-HF1 (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD pFUGW
    HypaCas9HypaCas9 (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD pFUGW
    evoCas9evoCas9 (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD pFUGW
    Sniper-Cas9Sniper-Cas9 (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSD pFUGW
    VQR variantVQR (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD pFUGW
    VRER variantVRER (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD pFUGW
    VRQR variantVRQR (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD pFUGW
    VRQR-HF1 variantVRQR-HF1 (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD pFUGW
    QQR1 variantQQR1 (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD pFUGW
    xCas9xCas9 (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD pFUGW
    SpCas9-NGSpCas9-NG (Synthetic)EFSBlasticidin Kim Prediction of the Sequence-Specific Cleavage Activity of Cas9 Variants Nat Biotechnol (2020). Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD pFUGW
    pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRESpCas9 (Other)Puromycin Sanjana High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette. lentiCRISPRv2
    pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRESpCas9-NG (Other)Puromycin Sanjana High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette. lentiCRISPRv2
    pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRExCas9 3.7 (Other)Puromycin Sanjana High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette. lentiCRISPRv2
    pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRExCas9-NG (Other)Puromycin Sanjana High-Throughput Screens of PAM-Flexible Cas9 Variants for Gene Knockout and Transcriptional Modulation. Cell Rep. 2020 Mar 3;30(9):2859-2868.e5. doi: 10.1016/j.celrep.2020.02.010. Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette. lentiCRISPRv2
    pORANGE LOX Cloning template vectorSpCas9 (Other)U6 MacGillavry ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 10.1371/journal.pbio.3000665. Cloning template for ORANGE-CAKE conditional knock-in constructs pORANGE
    pCMV-T7-SpCas9-P2A-EGFP (RTW3027)human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP JDS246
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpG-P2A-EGFP (RTW4177)human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpRY-P2A-EGFP (RTW4830)human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpCas9-VQR-P2A-EGFP (RTW3520)human codon optimized SpCas9-VQR with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VQR(D1135V/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpCas9-VRER-P2A-EGFP (RTW3160)human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP RTW3027
  • Tag / Fusion Protein
    • BPNLS-3xFLAG-P2A-EGFP (C terminal on insert)
  • pLentiCrispr-V2-mOrange (V2mO)pELF1aPuromycin ; mOrange flurescent marker Ajani An improved strategy for CRISPR/Cas9 gene knockout and subsequent wildtype and mutant gene rescue. PLoS One. 2020 Feb 13;15(2):e0228910. doi: 10.1371/journal.pone.0228910. eCollection 2020. Lenti Crispr/Cas9 with mOrange fluorescent marker pLentiCrispr-V2-mOrange (V2mO)
    Lenti-eCas9enhanced specificity Cas9 (1.1) (Synthetic)EF-1a corePuromycin Greber The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome Release at the Nuclear Pore Complex. Cell Rep. 2019 Dec 17;29(12):3785-3795.e8. doi: 10.1016/j.celrep.2019.11.064. Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold lentiCRISPR v2
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)
  • pX165-Sniper-Cas9Cas9 (Other) Zhang Highly Parallel Profiling of Cas9 Variant Specificity. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. Expresses Sniper Cas9 in mammalian cells pX165
    pX165-LZ3 Cas9Cas9 (Other) Zhang Highly Parallel Profiling of Cas9 Variant Specificity. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. Expresses LZ3 Cas9 in mammalian cells pX165
    pX165-HiFi Cas9Cas9 (Other) Zhang Highly Parallel Profiling of Cas9 Variant Specificity. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. Expresses HiFi Cas9 in mammalian cells pX165
    pX165-eSpCas9Cas9 (Other) Zhang Highly Parallel Profiling of Cas9 Variant Specificity. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. Expresses eSpCas9 in mammalian cells pX165
    pX165-Cas9-HF1Cas9 (Other) Zhang Highly Parallel Profiling of Cas9 Variant Specificity. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. Expresses Cas9-HF1 in mammalian cells pX165
    pX165-HypaCas9Cas9 (Other) Zhang Highly Parallel Profiling of Cas9 Variant Specificity. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. Expresses HypaCas9 in mammalian cells pX165
    pX165-xCas9Cas9 (Other) Zhang Highly Parallel Profiling of Cas9 Variant Specificity. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. Expresses xCas9 in mammalian cells pX165
    pX165-evoCas9Cas9 (Other) Zhang Highly Parallel Profiling of Cas9 Variant Specificity. Mol Cell. 2020 Mar 17. pii: S1097-2765(20)30143-X. doi: 10.1016/j.molcel.2020.02.023. Expresses evoCas9 in mammalian cells pX165
    pDEST-Cas9-tdTomatoCas9 (Other) Okano A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology. PLoS One. 2019 Aug 27;14(8):e0221164. doi: 10.1371/journal.pone.0221164. eCollection 2019. Mutisite Gateway mediated vector construction pUC
    XClone_Cas9_KRAB_T2A_BFPdCas9 (Other)Blasticidin Johnson Tunable and temporal expression control of Cas9 (unpublished) Tunable and temporal expression control of dCas9 XLone-GFP
    XClone-Cas9-Cronus-T2A-BFPCas9 (Other)Blasticidin Johnson Tunable and temporal expression control of Cas9 (unpublished) Tunable and temporal expression control of Cas9 XLone-GFP
    px551-mSncg-0.27kb-HA-SpCas9Cas9Truncated mSncg (0.27kb) Hu Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing in Mammalian Retinal Ganglion Cells. J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 10.1523/JNEUROSCI.0102-20.2020. Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells pX551
    pQCiGFP McComb Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of large genetic payloads for rapid cell engineering (unpublished) Self-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites) pQCi
    pJ607-CMV-GFP-2A-Cas9GFP-2A-Cas9, HygRCMV, SV40 promoterHygromycin Grav Multi-copy targeted integration for accelerated development of high-producing CHO cells. ACS Synth Biol. 2020 Aug 24. doi: 10.1021/acssynbio.0c00322. GFP-2A-Cas9 (CHO codon-optimized) pJ607
    Sc++Sc++ (Other)EF1ɑ Jacobson An engineered ScCas9 with broad PAM range and high specificity and activity. Nat Biotechnol. 2020 May 11. pii: 10.1038/s41587-020-0517-0. doi: 10.1038/s41587-020-0517-0. Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells Adapted from pCMV_AncBE4max (#112094)
    iSpyMaciSpyMac (Other)EF1ɑ Jacobson A Cas9 with PAM recognition for adenine dinucleotides. Nat Commun. 2020 May 18;11(1):2474. doi: 10.1038/s41467-020-16117-8. Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells Adapted from pCMV_AncBE4max (#112094)
    Cas9 CFPspCas9 Carroll Lenitviral sgRNA and Cas9 constructs (unpublished) Lentiviral Cas9 expression with CFP marker Addgene #108100
    Cas9 NGFRspCas9 Carroll Lenitviral sgRNA and Cas9 constructs (unpublished) Lentiviral Cas9 expression with NGFR marker Addgene #108100
    Lenti Cas9/SB100SB100X-P2A-spCas9 Carroll Lenitviral sgRNA and Cas9 constructs (unpublished) Lentiviral expression of Cas9 and SB100 LentiV
    pRDA_085SpCas9 (Synthetic) Root Massively parallel assessment of human variants with base editor screens (unpublished) Lentiviral expression of SpCas9; P2A site provides mKate2 pXPR

    Base Edit

    Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert adenine to inosine, which is replaced by guanosine to create A->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik lab, uses an MS2 hairpin-tagged gRNA to recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed evolution.

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pCMV-BE1BE1 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE1 with C-terminal NLS in mammalian cells CMV
    pCMV-BE2BE2 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE2 in mammalian cells CMV
    pCMV-BE3BE3 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE3 in mammalian cells CMV
    pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)SpCas9 (Other)pCMVNeomycin (select with G418) Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells pcDNA3.1
    MO91-dCas9-AID(P182X)dCas9-AID P182X (Synthetic)MSCVPuromycin Chang Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10. Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone. M091
    pJL-SaBE3SaBE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses SaBE3 in mammalian cells CMV
    pJL-SaKKH-BE3SaKKH-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses SaKKH-BE3 in mammalian cells [Edit] CMV
    pBK-VQR-BE3VQR-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses VQR-BE3 in mammalian cells CMV
    pBK-EQR-BE3EQR-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses EQR-BE3 in mammalian cells CMV
    pBK-VRER-BE3VRER-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses VRER-BE3 in mammalian cells CMV
    pBK-YE1-BE3YE1-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YE1-BE3 in mammalian cells CMV
    pBK-EE-BE3EE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses EE-BE3 in mammalian cells CMV
    pBK-YE2-BE3YEE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YE2-BE3 in mammalian cells CMV
    pBK-YEE-BE3YEE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YEE-BE3 in mammalian cells CMV
    pGH335_MS2-AID*Δ-HygroMS2-AID*Δ and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker Addgene #61426
    pGH183_MS2-AIDΔDead-HygroMS2-AIDΔDead and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker Addgene #61426
    pGH156_MS2-AID-HygroMS2-AID and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker Addgene #61426
    pGH153_MS2-AIDΔ-HygroMS2-AIDΔ and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker Addgene #61426
    pCMV-HF-BE3HF-BE3 (Homo sapiens)CMV Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790. Expresses HF-BE3 with C-terminal NLS in mammalian cells pCMV
    pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLSrAPOBEC1-nCas9 (D10A)-UGI-NLS (Rattus norvegicus)T7 Kim Highly efficient RNA-guided base editing in mouse embryos. Nat Biotechnol. 2017 Feb 27. doi: 10.1038/nbt.3816. Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells. pET28a
    BE4BE (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE4 in mammalian cells pCMV
    AID-BE3activation induced cytidine deaminase (Homo sapiens)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses AID-BE3 in mammalian cells pCMV AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    CDA1-BE3CDA1-BE3 (Synthetic)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses CDA1-BE3 in mammalian cells pCMV
    SaBE4SaBE4 (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE4 in mammalian cells pCMV
    BE4-GamBE4-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE4-Gam in mammalian cells pCMV
    BE3-GamBE3-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE3-Gam in mammalian cells pCMV
    SaBE4-GamSaBE4-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE4-Gam in mammalian cells pCMV
    SaBE3-GamSaBE3-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE3-Gam in mammalian cells pCMV
    pCMV-eBE-S1eBE-S1 (Rattus norvegicus)CMV Chen Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. Expresses eBE-S1 in mammalian cells pCMV
    pCMV-eBE-S3eBE-S3 (Rattus norvegicus)CMV Chen Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. Expresses eBE-S3 in mammalian cells pCMV
    pCMV-ABE6.3ABE6.3 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE6.3 in mammalian cells pBR322/CMV
    pCMV-ABE7.8ABE7.8 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.8 in mammalian cells pBR322/CMV
    pCMV-ABE7.9ABE7.9 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.9 in mammalian cells pBR322/CMV
    pCMV-ABE7.10ABE7.10 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.10 in mammalian cells pBR322/CMV
    pCMV-dCpf1-BE0dLbCpf1-BE0 (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE0 in mammalian cells CMV
    pCMV-dLbCpf1-BE0ΔiNLSdLbCpf1-BE0ΔiNLS (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells CMV
    pCMV-dCpf1-BEdLbCpf1-BE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE in mammalian cells CMV
    pCMV-dCpf1-BE-YEdLbCpf1-BE-YE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE-YE in mammalian cells CMV
    pCMV-dCpf1-BE-YEEdLbCpf1-BE-YEE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE-YEE in mammalian cells CMV
    pCMV-dCpf1-eBEdLbCpf1-eBE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-eBE in mammalian cells CMV
    pCMV-dCpf1-eBE-YEdLbCpf1-eBE-YE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-eBE-YE in mammalian cells CMV
    pCMV-ABEABE7.10 (Synthetic)CMV Huang Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7. For expression of ABE7.10 in mammalian cells PBR322
    pWT063aTRESG-BE3 (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
    pWT065a(TCF-LEF)7-BE3 (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
    pWT065c(TCF-LEF)7-BE3-P2A-Luc (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
    xCas9(3.7)-BE3xCas9(3.7)-BE3 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-BE3 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.7)-BE4xCas9(3.7)-BE4 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-BE4 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.7)-ABE(7.10)xCas9(3.7)-ABE(7.10) (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-ABE pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.6)-BE3xCas9(3.6)-BE3 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-BE3 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.6)-BE4xCas9(3.6)-BE4 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-BE4 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.6)-ABE(7.10)xCas9(3.6)-ABE(7.10) (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-ABE pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • A3Ai-Cas9n-UGI-NLSApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3A
    A3Bi-ctd E255A -Cas9n-UGI-NLSApolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
    A3Ai E72A-Cas9n-UGI-NLSApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-BE3-2XBE3-2X (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-2X construct (not codon optimized) pCMV
    pCMV-BE3-FNLSBE3-FNLS (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-FNLS construct (not codon optimized) pCMV
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-BE3-P2A-PuroBE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3 (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE3RA-P2A-PuroBE3RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-2X-P2A-PuroBE3RA-2X (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-FNLS-P2A-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-BE3-PGK-PuroBE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3 (not codon optimized) pLL3.3
    pLenti-BE3RA-PGK-PuroBE3RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) pLL3.3
    pLenti-FNLS-PGK-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) pLL3.3
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-TRE3G-BE3-PGK-PuroBE3 (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of BE3 (not codon optimized) pLL3.3
    pLenti-TRE3G-BE3RA-PGK-PuroBE3RA (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized) pLL3.3
    pLenti-TRE3G-FNLS-PGK-PuroBE3RA-FNLS (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized) pLL3.3
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-BE3-P2A-GFP-PGK-PuroBE3 (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE3RA-P2A-GFP-PGK-PuroBE3RA (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-FNLS-P2A-GFP-PGK-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-xBE3-P2A-PuroxCas9(3.7)-BE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells Adapted from lentiCRISPRv1
    pLenti-xFNLS-P2A-PuroxFNLS(3.7) (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-xF2X-P2A-PuroxFNLS-2X(3.7) (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pCMV_BE4maxBE4max (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_AncBE4maxAncBE4max (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_ABEmaxABEmax (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
    pCMV_BE4max_3xHABE4max_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
  • pCMV_AncBE4max_3xHAAncBE4max_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
  • pCMV_ABEmax_3xHAABEmax_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
  • pCMV_BE4max_P2A_GFPBE4max_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_AncBE4max_P2A_GFPAncBE4max_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_ABEmax_P2A_GFPABEmax_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
    pLL1 sgRNA(MS2)-MS2-AIDmonoAID mono mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AIDmono pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL2 sgRNA(MS2)-MS2-AID3cAID mono 3c mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AID3c pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL3 sgRNA(MS2)-MS2-AID3fAIDmono 3f mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AID3f pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL4 sgRNA(MS2)-MS2-hA3ahuman Apobec3a (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pLL5 sgRNA(MS2)-MS2-hA3a D131Ahuman Apobec3a D131A mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a D131A pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pLL6 sgRNA(MS2)-MS2-hA3a D131Ehuman Apobec3a D131E mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a D131E pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-BE3-FNLS(RA)BE3-FNLS (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-FNLS construct (codon optimized) pCMV
    pLenti-BE4RA-P2A-PuroBE4RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE4GamRA-P2A-PuroBE4GamRA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-ABE7.10-P2A-PuroABE7.10 (Synthetic)EF1sPuromycin Dow Dow Lab Unpublished Base Editors (unpublished) Lentivirus for constitutive expression of ABE7.10 (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-ABERA-P2A-PuroABERA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of ABE7.10 RA (codon optimized) Adapted from lentiCRISPRv1
    pLenti-xBE4Gam-P2A-PuroxCas9(3.7)-BE4Gam (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pAAV-ABE-NT-sgRNAABE-NT-sgRNA (Other) Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA pAAV
    rat APOBEC1-EGFPapolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Rattus norvegicus)CMV promoterNeomycin (select with G418) Conticello Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells. RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033. plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein pEGFP-N1 Apobec1 REPR, apobec-1
    pEGFP mCherry-ApoB-EGFPmCherry-apoB-EGFP (Synthetic)CMV promoterNeomycin (select with G418) Conticello Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells. RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033. Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP pEGFP-N1
    pAAV-ABE-CTABE-CT Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. AAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9 pAAV
    pST1374-scFv-APOBEC-UGI-GB1scFv-rAPOBEC1-UGI-GB1 (Rattus norvegicus)CMV Huang BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4. Expresses scFv-rAPOBEC1-UGI in mammalian cells pST1374
    p3s-ABE7.10ABE 7.10 (Other)CMV Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized pCDNA3.1
    pCMV-hA3A-BE3hA3A-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3 in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3B-BE3hA3B-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3B-BE3 in mammalian cells CMV APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
    pCMV-hA3C-BE3hA3C-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3C-BE3 in mammalian cells CMV APOBEC3C A3C, APOBEC1L, ARDC2, ARDC4, ARP5, PBI, bK150C2.3
    pCMV-hA3D-BE3hA3D-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3D-BE3 in mammalian cells CMV APOBEC3D A3D, APOBEC3DE, APOBEC3E, ARP6
    pCMV-hA3F-BE3hA3F-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3F-BE3 in mammalian cells CMV APOBEC3F A3F, ARP8, BK150C2.4.MRNA, KA6
    pCMV-hA3G-BE3hA3G-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3G-BE3 in mammalian cells CMV APOBEC3G A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1
    pCMV-hA3H-BE3hA3H-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3H-BE3 in mammalian cells CMV APOBEC3H A3H, ARP-10, ARP10
    pCMV-hAID-BE3hAID-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hAID-BE3 in mammalian cells CMV AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pCMV-hA1-BE3hA1-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA1-BE3 in mammalian cells CMV APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    pCMV-mA3-BE3mA3-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mA3-BE3 in mammalian cells CMV Apobec3 Apobec, Arp3, BC003314, Cem15, Gm20117, Rf, Rfv, Rfv3
    pCMV-mAID-BE3mAID-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mAID-BE3 in mammalian cells CMV Aicda Aid, Arp2
    pCMV-mA1-BE3mA1-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mA1-BE3 in mammalian cells CMV Apobec1 Cdar1
    pCMV-cAICDA-BE3cAICDA-BE3 (Other)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses cAICDA-BE3 in mammalian cells CMV aicda aid
    pCMV-hA3A-BE3-D131YhA3A-BE3-D131Y (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-D131Y in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-Y132DhA3A-BE3-Y132D (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-Y132D in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-C101ShA3A-BE3-C101S (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-C101S in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-C106ShA3A-BE3-C106S (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-C106S in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-Y130FhA3A-BE3-Y130F (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-Y130F in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized AsBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
  • pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized AsBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
  • pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
  • pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
  • pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.3 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
  • pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
  • pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized LbBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
  • pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4) (Synthetic)CAG Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized LbBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
  • PB-BE3-NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    PB-BE3-2NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    PB-BE3-3NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    A3Bi-Cas9n-UGIAPOBEC3B L1 intron-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction BE3
    A3C-Cas9n-UGIAPOBEC3C-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3C BE3
    A3D-Cas9n-UGIAPOBEC3D-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3D BE3
    A3F-Cas9n-UGIAPOBEC3F-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3F BE3
    A3G-Cas9n-UGIAPOBEC3G-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3G BE3
    A3H HapI-Cas9n-UGIAPOBEC3H Haplotype I-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype I BE3
    A3H HapII-Cas9n-UGIAPOBEC3H Haplotype II-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype II BE3
    A3H HapII R175/6E-Cas9n-UGIAPOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE BE3
    CP1012-CBEmaxrApobec1-nSpCas9 (CP1012) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1028-CBEmaxrApobec1-nSpCas9 (CP1028) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1041-CBEmaxrApobec1-nSpCas9 (CP1041) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1249-CBEmaxrApobec1-nSpCas9 (CP1249) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1300-CBEmaxrApobec1-nSpCas9 (CP1300) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1012-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1012) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1028-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1028) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1041-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1041) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1249-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1249) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1300-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1300) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    VRQR-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (VRQR) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    VRER-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (VRER) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    xABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (xCas9) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    SaABEmaxecTadA-ecTadA*(7.10)-nSaCas9 (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    SaKKH-ABEmaxecTadA-ecTadA*(7.10)-nSaCas9 (KKH) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    pSI-Target-AID-NGnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) Nureki Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129. Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells. pcDNA3.1
    pLV302N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE) (Rattus norvegicus)CMV Schwank Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8. Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells px601
    pLV312.3C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3 (Rattus norvegicus)CMV Schwank Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8. Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells px601
    pcDNA3.1(-)-HF2-BE2HF2-BE2 (Synthetic)CMVNeomycin (select with G418) Huang Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5. Expresses HF2-BE2 in mammalian cells pcDNA3.1(-)
    pcDNA3.1(-)-HF2-BE3HF2-BE3 (Synthetic)CMVNeomycin (select with G418) Huang Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5. Expresses HF2-BE3 in mammalian cells pcDNA3.1(-)
    pcDNA3.1(-)-ABE7.10ABE7.10 (Synthetic)CMVNeomycin (select with G418) Huang Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z. Expresses ABE7.10 in mammalian cells pcDNA3.1(-)
    CAG-FNLS-T2A-EGFP-ires-puroFNLS-T2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance. PyCAG
    CAG-ABEmax-P2A-EGFP-ires-puroABEmax-P2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance. PyCAG
    CAG-xFNLS-T2A-EGFP-ires-puroxFNLS-T2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance. PyCAG
    pBT44c intein-dCas9-ugi fusion (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. BE PACE selection, express C-intein-Cas9-ugi fusion pBT38
    pBT276 CDA1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express CDA1-BE4max in mammalian cells pBT195
    pBT277 evoCDA1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express evoCDA1-BE4max in mammalian cells pBT195
    pBT281 evoAPOBEC1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express evoAPOBEC1-BE4max in mammalian cells pBT195
    nCas9-UGI-NLS-P2A-EGFP (pJUL1001)nCas9-UGI-NLS-P2A-EGFP (Synthetic)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1). CAG
    BE3-P2A-EGFP (pJUL977)BE3-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP. CAG
    BE3(E63Q)-P2A-EGFP (pRZ189)BE3(E63Q)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant). CAG
    SECURE BE3(R33A)-P2A-EGFP (pJUL1085)BE3(R33A)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A). CAG
    SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)BE3(R33A/K34A)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A). CAG
    NG-ABEmaxTadA-TadA(evo)-Cas9-NG Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV
    dABEdABE7.10 (Other) Church Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239. Expresses dead Cas9 (dCas9)-ABE7.10 pCMV_ABE7.10
    dCBE4-gamdCBE4-gam (Other) Church Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239. Expresses dead Cas9 (dCas9)-CBE4-gam pCMV
    pBT371CDA1-BE4max-NG (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. Expresses CDA1-BE4max-NG in mammalian cells pBT195
    pBT372evoCDA1-BE4max-NG (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. Expresses evoCDA1-BE4max-NG in mammalian cells pBT195
    pBT375evoAPOBEC1-BE4max-NG (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. Expresses evoAPOBEC1-BE4max-NG in mammalian cells pBT195
    pCMV-ABEmaxAWABEmax(TadA E59A, TadA* V106W) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. Expresses ABEmaxAW in mammalian cells CMV
    pCMV-ABEmax(TadA E59A)ABEmax(TadA E59A) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. Expresses ABEmax(TadA E59A) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*E59A)ABEmax(TadA E59A TadA* E59A) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*V106M)ABEmax(TadA E59A TadA* V106M) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*V106M) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*V106Q)ABEmax(TadA E59A TadA* V106Q) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*V106Q) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*V106F)ABEmax(TadA E59A TadA* V106F) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*V106F) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*D108W)ABEmax(TadA E59A TadA* D108W) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*D108W) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*D108F)ABEmax(TadA E59A TadA* D108F) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*D108F) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*D108Q)ABEmax(TadA E59A TadA* D108Q) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*D108M)ABEmax(TadA E59A TadA* D108M) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*D108M) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*R47Q)ABEmax(TadA E59A TadA* R47Q) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*R47Q) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*R47F)ABEmax(TadA E59A TadA* D108F) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*R47F) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*R47W)ABEmax(TadA E59A TadA* R47W) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*R47W) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*R47M)ABEmax(TadA E59A TadA* R47M) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*R47M) in mammalian cells CMV
    pCMV-ABEmax(TadA, TadA*E59A)ABEmax(TadA E59A TadA* E59A) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA, TadA*E59A) in mammalian cells CMV
    pRS0045TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI (Synthetic)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian Target-ACE expression vector pcDNA3.1
    pRS0035nCas9-PmCDA1-UGI (Synthetic)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian Target-AID expression vector pcDNA3.1
    nCas9-UGI control for Target-AID (pRZ1047)NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Other)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1). CAG
    Target-AID (pRZ762)NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP (Other)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP. CAG
    miniABEmax (pRZ900) bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)CMV Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain). CMV
    SECURE miniABEmax-K20A/R21A (pJUL1774) bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)CMV Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations). CMV
    SECURE miniABEmax-V82G (pJUL1828) bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)CMV Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation). CMV
    hA3A-BE3 (pRZ215)hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
    eA3A-BE3 (pRZ206)hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
    hAID-BE3 (pRZ352)hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
    N-4-BELjCDA1L1_4 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9 Px330
    N-8-BELpCDA1L1_1 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9 Px330
    C-1-BEPmCDA1L1_4 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9 Px330
    C-7-BELpCDA1L1_1 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9 Px330
    C-11-BELpCDA1L2_2 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9 Px330
    C-12-BELjCDA1L2_1 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9 Px330
    C-13-BELpCDA1L1_4 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9 Px330
    AIDx-ID-1058nSpyCas9-hAIDx-ID-1058-UGI (Homo sapiens)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus pcDNA3.1(+)
    BE3-NLS-NLS-P2A-BLASTBE3-2XNLS (Rattus norvegicus)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance pcDNA3.1(+)
    S2 1058 AIDx no UGInSpyCas9-hAIDx-ID-1058 (Homo sapiens)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor. pcDNA3.1(+)
    S2 1058 APOBEC3 no UGInSpyCas9-rAPOBEC3-ID-1058 (Rattus norvegicus)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor. pcDNA3.1(+)
    S1 1029 CP APOBEC3 no UGIrAPOBEC3-nSpyCas9 aa 1029 (Rattus norvegicus)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus pcDNA3.1(+)
    S1 1029 AIDx with UGIhAIDx-nSpyCas9 aa-UGI (Rattus norvegicus)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus pcDNA3.1(+)
    pCMV-ABEMAX-NLS-P2A_BLASTABEMax-2XNLS (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance pcDNA3.1(+)
    S2 1058 ABEMaxnSpyCas9-ABEMax aa 1058 (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9 pcDNA3.1(+)
    S1 1029 CP ABEMax strategy 1ABEMax-nSpyCas9 aa 1029 (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus pcDNA3.1(+)
    S1 1029 CP ABEMax strategy 2WT Tada-nSpyCas9 aa 1029-evolved Tada (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus) pcDNA3.1(+)
    S1 1029 CP ABEMax strategy 31029 CP nSpyCas9 ABEMax (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus pcDNA3.1(+)
    S1 1029 miniABEMax V82G strategy1mini ABEMax 1029 nSpyCas9 (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus pcDNA3.1(+)
    S2 1058 V82G ABEMax1058 nSpyCas9 V82G ABEMax (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9 pcDNA3.1(+)
    miniABEMax V82G wtminiABEMax nSpyCas9 (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSpyCas9 with N-terminal miniABEMax (V82G) pcDNA3.1(+)
    S1 1029 V82G ABEMax Strategy 31029 V82G ABEMax (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus pcDNA3.1(+)
    ABEMax nSaCas9ABEMax nSaCas9 (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus pcDNA3.1(+)
    miniABEMax V82G nSaCas9miniABEMax V82G nSaCas9 (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution pcDNA3.1(+)
    nSaCas9 D127 miniABEMax V82GnSaCas9 D127 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D127 ) pcDNA3.1(+)
    nSaCas9 D126 miniABEMax V82GnSaCas9 D126 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D126 ) pcDNA3.1(+)
    nSaCas9 E739 miniABEMax V82GnSaCas9 E739 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E739 ) pcDNA3.1(+)
    nSaCas9 E743 miniABEMax V82GnSaCas9 E743 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E743 ) pcDNA3.1(+)
    nSaCas9 E745 miniABEMax V82GnSaCas9 E745 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E745 ) pcDNA3.1(+)
    nSaCas9 F733 miniABEMax V82GnSaCas9 F733 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (F733 ) pcDNA3.1(+)
    nSaCas9 N730 miniABEMax V82GnSaCas9 N730 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N730 ) pcDNA3.1(+)
    nSaCas9 E125 miniABEMax V82GnSaCas9 E125 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E125 ) pcDNA3.1(+)
    nSaCas9 E131 miniABEMax V82GnSaCas9 E131 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E131 ) pcDNA3.1(+)
    nSaCas9 L132 miniABEMax V82GnSaCas9 L132 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (L132 ) pcDNA3.1(+)
    nSaCas9 N130 miniABEMax V82GnSaCas9-ABEMax(V82G) N130 (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N130 ) pcDNA3.1(+)
    nSaCas9 E735 miniABEMax V82GnSaCas9 E735 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E735 ) pcDNA3.1(+)
    nSaCas9 I744 miniABEMax V82GnSaCas9 I744 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 ) pcDNA3.1(+)
    nSaCas9 G129 miniABEMax V82GnSaCas9 G129 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (G129 ) pcDNA3.1(+)
    nSaCas9 H119 miniABEMax V82GnSaCas9-ABEMax(V82G) H119 (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (H119 ) pcDNA3.1(+)
    nSaCas9 M732 miniABEMax V82GnSaCas9 M732 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 ) pcDNA3.1(+)
    nSaCas9 M741 miniABEMax V82GnSaCas9 M741 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 ) pcDNA3.1(+)
    nSaCas9 N120 miniABEMax V82GnSaCas9 N120 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 ) pcDNA3.1(+)
    nSaCas9 P742 miniABEMax V82GnSaCas9 P742 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (P742 ) pcDNA3.1(+)
    nSaCas9 Q731 miniABEMax V82GnSaCas9 Q731 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q731 ) pcDNA3.1(+)
    nSaCas9 Q737 miniABEMax V82GnSaCas9 Q737 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q737 ) pcDNA3.1(+)
    nSaCas9 S740 miniABEMax V82GnSaCas9 S740 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (S740 ) pcDNA3.1(+)
    nSaCas9 T128 miniABEMax V82GnSaCas9 T128 miniABEMax V82G (Synthetic)Blasticidin Hewitt Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y. Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (T128 ) pcDNA3.1(+)
    SauriABEmax Wang A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar. Expresses ABEmax and nSauriCas9 pFS84-CMV-pfs
    SauriBE4max Wang A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar. Expresses AncBE4max and nSauriCas9 pFS84-CMV-pfs
    epi-ABEmaxBlasticidin Wang Yongming Wang lab plasmids (unpublished) Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
    epi-BE4maxBlasticidin Wang Yongming Wang lab plasmids (unpublished) Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
    epi-ABEmax-NGBlasticidin Wang Yongming Wang lab plasmids (unpublished) Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
    epi-BE4max-NGBlasticidin Wang Yongming Wang lab plasmids (unpublished) Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
    PB-XL-AncBE4maxAncBE4max_GFP (Synthetic)TRE3GBlasticidin Ye Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing. (2019) Nat Commun 10, 5353 Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max XLone-GFP (Addgene plasmid no. 96930)
    ABE8.8-mABE8.8-m Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.8-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.8-dABE8.8-d Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.8-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.13-mABE8.13-m Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.13-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.13-dABE8.13-d Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.13-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.17-mABE8.17-m Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.17-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.17-dABE8.20-d Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.17-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.20-m ABE8.20-m Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.20-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.20-dABE8.20-dCMV Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.20-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHASt1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)CAG, U6, CAG, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHASt1Cas9 LMD9:CNRZ1066 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)CAG, U6, CAG, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHASt1Cas9 LMD9:LMG18311 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)CAG, U6, CAG, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHASt1Cas9 LMD9:TH1477 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)CAG, U6, CAG, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), ABE7.10 (Homo sapiens)CAG, U6, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625)
    pLenti-FNLSNG-P2A-PuroFNLSNG (Synthetic)EF1sPuromycin Dow GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pLenti-FNLSHF1-P2A-PuroFNLSHF1 (Synthetic)EF1sPuromycin Dow GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pLenti-FNLSHiFi-P2A-PuroFNLSHiFi (Synthetic)EF1sPuromycin Dow GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pLenti-FNLSHiFiNG-P2A-PuroFNLSHiFiNG (Synthetic)EF1sPuromycin Dow GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pCMV-BE4max-NRRHBE4max-NRRH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses BE4max-NRRH in mammalian cells pBR322 CMV
    pCMV-BE4max-NRTHBE4max-NRTH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses BE4max-NRTH in mammalian cells pBR322 CMV
    pCMV-BE4max-NRCHBE4max-NRCH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses BE4max-NRCH in mammalian cells pBR322 CMV
    pCMV-ABEmax-NRTHABEmax-NRTH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses ABEmax-NRTH in mammalian cells pBR322 CMV
    pCMV-ABEmax-NRCHABEmax-NRCH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses ABEmax-NRCH in mammalian cells pBR322 CMV
    CMV_Npu-BE3.9max N-terminalNpu-BE3.9max_N-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter pCMV
    CMV_Npu-BE3.9max C-terminalNpu-BE3.9max_C-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter pCMV
    CMV_Npu-ABEmax N-terminalNpu-ABEmax_N-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter pCMV
    CMV_Npu-ABEmax C-terminalNpu-ABEmax_C-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter pCMV
    Cbh_v5 AAV-ABE N-terminalv5 AAV-ABE N-terminal (Synthetic)Cbh Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter px601
    Cbh_v5 AAV-ABE C-terminalv5 AAV-CBE C-terminal; U6-protospacer (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA px601
    CMV_Npu-saBE3.9max N-terminalNpu-saBE3.9max_N-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter pCMV
    CMV_Npu-saBE3.9max C-terminalNpu-saBE3.9max_C-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter pCMV
    CMV_Npu-saBE3.9max_KKH C-terminalNpu-saBE3.9max_KKH_C-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter pCMV
    YE1-BE4maxrAPOBEC1(YE1)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • YE2-BE4maxrAPOBEC1(YE2)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • YEE-BE4maxrAPOBEC1(YEE)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • EE-BE4maxrAPOBEC1(EE)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • YE1-BE4max-NGrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • YE1-BE4max-CP1028rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • AALN-BE4maxrAPOBEC1(AALN)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pEF-BE4GamBE4Gam (Synthetic)Ef1a Wang A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713. Expression of BE4am-Cas9 Base-editor from an EF1a promoter. pEF
    pEF-AncBE4maxAncBE4max (Synthetic)Ef1a Wang A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713. Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter. pEF
    pCMV-BE4-PpAPOBEC1 H122ABE4-PpAPOBEC1 H122A (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-PpABOBEC1H122A pCMV
    pCMV-BE4-RrA3FBE4-RrA3F (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-RrA3F pCMV
    pCMV-BE4-RrA3F F130LBE4-RrA3F F130L (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-RrA3F F130L pCMV
    pCMV-BE4-AmAPOBEC1BE4-AmAPOBEC1 (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-AmABOBEC1 pCMV
    pCMV-BE4-SsAPOBEC3BBE4-SsAPOBEC3B (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-SsAPOBEC3B pCMV
    pCMV-BE4-SsAPOBEC3B R54QBE4-SsAPOBEC3B R54Q (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q pCMV
    pCMV-BE4-PpAPOBEC1 R33ABE4-PpAPOBEC1 R33A (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-PpABOBEC1 R33A pCMV
    pCMV-BE4-PpAPOBEC1BE4-PpAPOBEC1 (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-PpABOBEC1 pCMV
    ABE8eecTadA(8e)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    ABE8e-dimerecTadA(WT)-ecTadA(8e)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    NG-ABE8eecTadA(8e)-nSpCas9 (NG) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    CP1028-ABE8eecTadA(8e)-nSpCas9 (CP1028) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    CP1041-ABE8eecTadA(8e)-nSpCas9 (CP1041) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    ABE8e(TadA-8e V82G)ecTadA(8e V82G)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    ABE8e(TadA-8e V106W)ecTadA(8e V106W)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    ABE8e(TadA-8e K20A R21A)ecTadA(8e K20A R21A)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    NG-ABE8-dimerecTadA(WT)-ecTadA(8e)-nSpCas9 (NG) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    CP1028-ABE8e-dimerecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    CP1041-ABE8e-dimerecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaABE8eecTadA(8e)-nSaCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaABE8e-dimerecTadA(WT)-ecTadA(8e)-nSaCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaKKH-ABE8eecTadA(8e)-nSaCas9 (KKH) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaKKH-ABE8e-dimerecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    LbABE8eecTadA(8e)-dLbCas12a (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    LbABE8e-dimerecTadA(WT)-ecTadA(8e)-dLbCas12a (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    enAsABE8eecTadA(8e)-dAsCas12a (En) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    enAsABE8e-dimerecTadA(WT)-ecTadA(8e)-dAsCas12a (En) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    LbABE8e(TadA-8e V106W)ecTadA(8e V106W)-dLbCas12a (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaABE8e(TadA V106W)ecTadA(8e V106W)-nSaCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    pBT44cinteinC-dSpCas9-UGI (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) P2 pCMV with a BR322 origin
    H446 EF1a RA-ABE6.3 p2A PuroABE RA6.3 (Other)EF1aPuromycin Xue Adenine base editing in an adult mouse model of tyrosinaemia. Nat Biomed Eng. 2019 Feb 25. pii: 10.1038/s41551-019-0357-8. doi: 10.1038/s41551-019-0357-8. Lentiviral expression of ABE6.3. lenti
    pCMV-Target-ACEmaxBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS (Synthetic)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian Target-ACEmax expression vector (pSI-625) pcDNA3.1
    pCMV-ACBEmaxBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS (Rattus norvegicus)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian ACBEmax expression vector (pSI-632) pcDNA3.1
    pCMV-Target-AIDmaxBi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS (Synthetic)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian Target-AIDmax expression vector (pSI-626) pcDNA3.1
    pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP pRZ33
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP pRZ33
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)human codon optimized CBE4max xCas9(3.7) with P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP pRZ33
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)human codon optimized CBE4max SpCas9-NG with P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP pRZ33
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP pCMV_ABEmax_P2A_GFP
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCAG-BE4max-P2A-EGFP (pRZ33)Human codon optimized pCAG BE4max with P2A-EGFP (Synthetic)CAG Joung Joung Lab Plasmids (unpublished) CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP pCAG backbone
    SPACE (pRZ1813)SPACE (Other)CMV Joung A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
    SPACE-ΔUGI (pRZ1836)SPACE-ΔUGI (Other)CMV Joung A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP CMV
    SPACE-NG (pRZ5150)SPACE_NG (Other)CMV Joung A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
    SPACE-VRQR (pRZ5137)SPACE_VRQR (Other)CMV Joung A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
    SECURE BE4max(R33A) (pBM608)BE4max(R33A) (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP CMV
    CGBE1 (pRZ3885)eUNG-BE4max(R33A)-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP CMV
    miniCGBE1 (pBM1219)BE4max(R33A)-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP CMV
    CGBE1-VRQR (pBM1192)eUNG-BE4max(R33A)_VRQR-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP CMV
    miniCGBE1-VRQR (pBM1173)BE4max(R33A)_VRQR-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP CMV
    CGBE1-NG (pBM1246)eUNG-BE4max(R33A)_NG-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP CMV
    miniCGBE1-NG (pBM1182)BE4max(R33A)_NG-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP CMV
    p2T-CMV-ABEmax-BlastRABE7.10-nSpCas9 (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. A-to-G base editor p2T-CMV-BlastR
    p2T-CMV-ABEmaxCP1041-BlastRABE7.10-nSpCas9 CP1041 (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. A-to-G base editor p2T-CMV-BlastR
    p2T-CMV-BE4max-BlastRBE4-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-EA-BE4max-NG-BlastRBE4 H47E+S48A-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. High purity C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-BE4maxCP1028-BlastRBE4-nSpCas9 CP1028-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-AIDmax-BlastRAID-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-CDAmax-BlastRCDA-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-evoAmax-BlastRevoA-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-eA3Amax-BlastReA3A-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-eA3A-BE5-NG-BlastReA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. High purity C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-eA3AmaxNG-BlastReA3A-nSpCas9 NG-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor, NG PAM p2T-CMV-BlastR
    p2T-CMV-eA3Amax-T31A-NG-BlastReA3A-BE4 T31A-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T/G/A base editor p2T-CMV-BlastR
    CMV-YE1-BE3-FNLS-CMV-mCherryYE1-BE3-FNLS (Synthetic)CMV Yang A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Nat Methods. 2020 May 18. pii: 10.1038/s41592-020-0832-x. doi: 10.1038/s41592-020-0832-x. Express YE1-BE3-FNLS unspecified

    Nick

    CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR).

    Plasmid Gene/Insert Promoter Selectable Marker Publication Hidden Extra Search Info
    hCas9_D10ACas9_D10ACMVRNA-Guided Human Genome Engineering via Cas9. Science. 2013 Jan 3. </