A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects of CRISPR.
Browse, sort, or search the tables below for CRISPR-FokI plasmids.
Plasmids are available for expression in mammalian systems and Drosophila.
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