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CRISPR Plasmids: RNA Targeting


Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed cell death or dormancy for phage-infected bacterial cells so as to limit the spread of infection throughout the entire population.

Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation seen in bacteria.

CRISPR RNA targeting schematic


Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Bacterial

ID Plasmid Gene/Insert PI Publication

Plant

ID Plasmid Gene/Insert PI Publication


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