| pC014 - LwCas13a-msfGFP | LwCas13a-msfGFP (Homo sapiens) | EF1a | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses active LwCas13a for mammalian RNA targeting
lenti(AMP)
|
| pC015 - dLwCas13a-NF | dLwCas13a-eGFP-ZF-KRAB (Homo sapiens) | CAG | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
pCAGEN
|
| pC016 - LwCas13a guide expression backbone with U6 promoter | LwCas13a Guide site (Synthetic) | | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Backbone for cloning LwCas13a guides under U6 promoter
pUC19
|
| pC017 - LwCas13a guide expression backbone with tRNAval promoter | LwCas13a Guide site (Synthetic) | | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Backbone for cloning LwCas13a guides under tRNAval promoter
pUC19
|
| pC034 - LwCas13a-msfGFP-2A-Blast | LwCas13a-msfGFP (Homo sapiens) | EFS | Blasticidin |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker
pFUGW
|
| pC035 - dLwCas13a-msfGFP | dLwCas13a-msfGFP (Homo sapiens) | | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses catalytically inactive LwCas13a for mammalian RNA targeting
lenti(AMP)
|
| pC0040-LwaCas13a crRNA backbone | U6-LwaCas13aDR-BbsI-BbsI-polyT | U6 | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA
pUC19
|
| pC0043-PspCas13b crRNA backbone | U6-PspCas13b DR-BbsI-BbsI-polyT | | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
pUC19
|
| pC0044-EF1a-RanCas13b-NES-mapk | RanCas13b (Other) | | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
EF1a-lenti
|
| pC0045-EF1a-PguCas13b-NES-HIV | PguCas13b (Other) | | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
EF1a-lenti
|
| pC0046-EF1a-PspCas13b-NES-HIV | PspCas13b (Other) | | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm
EF1a-lenti
|
| pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A | PspCas13b (Other) | EF1a | |
Zhang
| RNA editing with CRISPR-Cas13.
Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
|
Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
Unknown
|
| pC0056 - LwCas13a-msfGFP-NES | LwCas13a-msfGFP-NES (Homo sapiens) | | |
Zhang
| RNA targeting with CRISPR-Cas13.
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
|
Expresses active LwaCas13a-NES for mammalian RNA targeting
lenti(AMP)
|
| pXR001: EF1a-CasRx-2A-EGFP | CasRx (Homo sapiens) | EF1A | GFP |
Hsu
| Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.
|
CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting
lenti(AMP)
|
| pXR002: EF1a-dCasRx-2A-EGFP | dCasRx (Homo sapiens) | | GFP |
Hsu
| Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.
|
Catalytically inactive dCasRx with 2A-EGFP
lenti(AMP)
|
| pAC1801_pmax-dCasRx | dCasRx (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses dCasRx; CAGGS promoter
pMAX
|
| pAC1802_pmax-RBFOX1N-dCasRx-C | RBFOX1N-dCasRx-C (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter
pMAX
|
| pAC1803_pmax-RBM38-dCasRx | RBM38-dCasRx (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter
pMAX
|
| pAC1804_pmax-dCasRx-RBM38 | dCasRx-RBM38 (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter
pMAX
|
| pAC1806_pmax-FKBP-dCasRx | FKBP-dCasRx (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter
pMAX
|
| pAC1807_pmax-dCasRx-FKBP | dCasRx-FKBP (Synthetic) | CAGGS | |
Cheng
| CRISPR Artificial Splicing Factors
bioRxiv 431064
|
Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter
pMAX
|
| Cmv d0 dPspCas13b-GGS-NYTHDF1 | dCas13b-GGS-NYTHDF1 | | |
Dickinson
| Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.
|
Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)
cmv d0
|
| Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) | dCas13b-GGS-NYTHDF2(100-200) | | |
Dickinson
| Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.
|
Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)
cmv d0
|
| Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) | dCas13b-GGS-NYTHDF1(100-200) | | |
Dickinson
| Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.
|
Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)
cmv d0
|
| Cmv d0 dPspCas13b-GGS-Sun | dCas13b-GGS-Sun | | |
Dickinson
| Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.
|
Cmv d0 dPspCas13b-GGS-Sun no-fusion construct
cmv d0
|
| pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag | dPspCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag | dPspCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag | dPguCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag | dPguCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag | dPspCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag | dPspCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag | dPspCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS | dPspCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
|
| pHAGE-NLS-dPspCas13b-3xmRuby3-NLS | dPspCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
|
| pHAGE-NLS-dPguCas13b-3xmRuby3-NLS | dPguCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
|
| pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag | dLwaCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag | dLbaCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag | dRanCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag | dEsCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag | dRfxCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag | dAdmCas13b (Other) | | |
Chen
| dCas13 imaging
Molecular Cell
|
overexpression in human cells
pHAGE-EF1a-IRES-puro
Tag
/ Fusion Protein- flag (N terminal on insert)
|
| pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE | PguCas13b (Homo sapiens) | | |
Sanjana
| Massively parallel Cas13 screens reveal principles for guide RNA design.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
|
Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
lentiCRISPRv2
Tag
/ Fusion Protein- NLS (C terminal on insert)
|
| pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE | PguCas13b (Homo sapiens) | | |
Sanjana
| Massively parallel Cas13 screens reveal principles for guide RNA design.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
|
Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
lentiCRISPRv2
Tag
/ Fusion Protein- NES (C terminal on insert)
|
| pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE | PspCas13b (Homo sapiens) | | |
Sanjana
| Massively parallel Cas13 screens reveal principles for guide RNA design.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
|
Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
lentiCRISPRv2
Tag
/ Fusion Protein- NLS (C terminal on insert)
|
| pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE | PspCas13b (Homo sapiens) | | |
Sanjana
| Massively parallel Cas13 screens reveal principles for guide RNA design.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
|
Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac
lentiCRISPRv2
Tag
/ Fusion Protein- NES (C terminal on insert)
|
| pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE | CasRx (Homo sapiens) | | |
Sanjana
| Massively parallel Cas13 screens reveal principles for guide RNA design.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
|
Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
lentiCRISPRv2
Tag
/ Fusion Protein- NLS (C terminal on insert)
|
| pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE | CasRx (Homo sapiens) | | |
Sanjana
| Massively parallel Cas13 screens reveal principles for guide RNA design.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
|
Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
lentiCRISPRv2
Tag
/ Fusion Protein- NES (C terminal on insert)
|
| pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast | CasRx (Homo sapiens) | | |
Sanjana
| Massively parallel Cas13 screens reveal principles for guide RNA design.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
|
Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus
lentiCas9-Blast
Tag
/ Fusion Protein- NLS-HA (C terminal on insert)
|
| pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE | hU6_Rfxcas13d-DR_BsmBI (Homo sapiens) | | |
Sanjana
| Massively parallel Cas13 screens reveal principles for guide RNA design.
Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
|
For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
lentiCRISPRv2
|
| CARPID BASU-dCasRx | BASU-dCasRx (Homo sapiens) | | |
Yan
| CRISPR-assisted detection of RNA-protein interactions in living cells.
Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 10.1038/s41592-020-0866-0.
|
express CARPID BASU-dCasRx fusion protein in mammalian cells
pXR001: EF1a-CasRx-2A-EGFP
|
| CARPID dCasRx-BASU | dCasRx-BASU (Homo sapiens) | | |
Yan
| CRISPR-assisted detection of RNA-protein interactions in living cells.
Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 10.1038/s41592-020-0866-0.
|
express CARPID dCasRx-BASU fusion protein in mammalian cells
pXR001: EF1a-CasRx-2A-EGFP
|
| AAV-GFAP-NLS-CasRx-NLS-FLAG | CasRx (Synthetic) | GFAP | |
Yang
| Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.
Cell. 2020 Apr 30;181(3):590-603.e16. doi: 10.1016/j.cell.2020.03.024. Epub 2020 Apr 8.
|
Express CasRx for AAV package
pAAV
|
| PB-CAG-CasRX-P2A-EGFP-BGH PolyA | CasRx (Synthetic) | CAG | |
Yang
| Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver.
Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2.
|
Express CasRx
PB
|
| dCas13d-dsRBD | BPNLS-dRfxCas13d-dsRBD-BPNLS-HA | CMV | Blasticidin |
Ting
| RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting.
Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 10.1073/pnas.2006617117.
|
BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
pLX304
|
| dCas13d-dsRBD-APEX2 | TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP | TRE3G | Puromycin |
Ting
| RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting.
Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 10.1073/pnas.2006617117.
|
TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
PB-TetON
|
| pCMV-dCas13-M3nls | dCas13-M3nls (Other) | | |
Liu
| Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase.
Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.
|
Targeted m6A RNA methylation in mammalian cells
pCMV with pBR322 origin
METTL3 IME4, M6A, MT-A70, Spo8, hMETTL3
|
| pCMV-dCas13-M3M14nes | dCas13-M3M14nes (Other) | | |
Liu
| Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase.
Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.
|
Targeted m6A RNA methylation in mammalian cells
pCMV with pBR322 origin
METTL3 IME4, M6A, MT-A70, Spo8, hMETTL3
METTL14 hMETTL14
|
| pU6-PspCas13b-gRNA-Actb1216 | PspCas13b gRNA targeting Actb 1216 (Synthetic) | | |
Liu
| Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase.
Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.
|
PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation
pU6 with pBR222 origin
|
| pCMV-dCas13-inactive M3nls | dCas13-inactive dCas13-inactive M3nls (Other) | | |
Liu
| Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase.
Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.
|
Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
pCMV with pBR322 origin
METTL3 IME4, M6A, MT-A70, Spo8, hMETTL3
|
| pCMV-dCas13-inactive M3M14nes | dCas13-inactive M3M14nes (Other) | | |
Liu
| Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase.
Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.
|
Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
pCMV with pBR322 origin
METTL3 IME4, M6A, MT-A70, Spo8, hMETTL3
METTL14 hMETTL14
|