CRISPR Plasmids: RNA Targeting

Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. In bacteria, once they have recognized and cleaved the target RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence just downstream of the sequence to be cleaved. This non-specific cleavage is thought to activate programmed cell death or dormancy for phage-infected bacterial cells so as to limit the spread of infection throughout the entire population.

Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does not exhibit the collateral RNA degradation seen in bacteria.

Mammalian

	Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
	pC014 - LwCas13a-msfGFP	LwCas13a-msfGFP (Homo sapiens)	EF1a		Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.	Expresses active LwCas13a for mammalian RNA targeting lenti(AMP)
	pC015 - dLwCas13a-NF	dLwCas13a-eGFP-ZF-KRAB (Homo sapiens)	CAG		Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.	Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging pCAGEN
	pC016 - LwCas13a guide expression backbone with U6 promoter	LwCas13a Guide site (Synthetic)			Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.	Backbone for cloning LwCas13a guides under U6 promoter pUC19
	pC017 - LwCas13a guide expression backbone with tRNAval promoter	LwCas13a Guide site (Synthetic)			Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.	Backbone for cloning LwCas13a guides under tRNAval promoter pUC19
	pC034 - LwCas13a-msfGFP-2A-Blast	LwCas13a-msfGFP (Homo sapiens)	EFS	Blasticidin	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.	Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker pFUGW
	pC035 - dLwCas13a-msfGFP	dLwCas13a-msfGFP (Homo sapiens)			Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.	Expresses catalytically inactive LwCas13a for mammalian RNA targeting lenti(AMP)
	pC0040-LwaCas13a crRNA backbone	U6-LwaCas13aDR-BbsI-BbsI-polyT	U6		Zhang	RNA editing with CRISPR-Cas13. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.	Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA pUC19
	pC0043-PspCas13b crRNA backbone	U6-PspCas13b DR-BbsI-BbsI-polyT			Zhang	RNA editing with CRISPR-Cas13. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.	For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac pUC19
	pC0044-EF1a-RanCas13b-NES-mapk	RanCas13b (Other)			Zhang	RNA editing with CRISPR-Cas13. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.	Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm EF1a-lenti
	pC0045-EF1a-PguCas13b-NES-HIV	PguCas13b (Other)			Zhang	RNA editing with CRISPR-Cas13. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.	Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm EF1a-lenti
	pC0046-EF1a-PspCas13b-NES-HIV	PspCas13b (Other)			Zhang	RNA editing with CRISPR-Cas13. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.	Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm EF1a-lenti
	pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A	PspCas13b (Other)	EF1a		Zhang	RNA editing with CRISPR-Cas13. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.	Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A). Unknown
	pC0056 - LwCas13a-msfGFP-NES	LwCas13a-msfGFP-NES (Homo sapiens)			Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.	Expresses active LwaCas13a-NES for mammalian RNA targeting lenti(AMP)
	pXR001: EF1a-CasRx-2A-EGFP	CasRx (Homo sapiens)	EF1A	GFP	Hsu	Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.	CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting lenti(AMP)
	pXR002: EF1a-dCasRx-2A-EGFP	dCasRx (Homo sapiens)		GFP	Hsu	Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.	Catalytically inactive dCasRx with 2A-EGFP lenti(AMP)
	pAC1801_pmax-dCasRx	dCasRx (Synthetic)	CAGGS		Cheng	CRISPR Artificial Splicing Factors bioRxiv 431064	Transient transfection; Expresses dCasRx; CAGGS promoter pMAX
	pAC1802_pmax-RBFOX1N-dCasRx-C	RBFOX1N-dCasRx-C (Synthetic)	CAGGS		Cheng	CRISPR Artificial Splicing Factors bioRxiv 431064	Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter pMAX
	pAC1803_pmax-RBM38-dCasRx	RBM38-dCasRx (Synthetic)	CAGGS		Cheng	CRISPR Artificial Splicing Factors bioRxiv 431064	Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter pMAX
	pAC1804_pmax-dCasRx-RBM38	dCasRx-RBM38 (Synthetic)	CAGGS		Cheng	CRISPR Artificial Splicing Factors bioRxiv 431064	Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter pMAX
	pAC1806_pmax-FKBP-dCasRx	FKBP-dCasRx (Synthetic)	CAGGS		Cheng	CRISPR Artificial Splicing Factors bioRxiv 431064	Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter pMAX
	pAC1807_pmax-dCasRx-FKBP	dCasRx-FKBP (Synthetic)	CAGGS		Cheng	CRISPR Artificial Splicing Factors bioRxiv 431064	Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter pMAX
	Cmv d0 dPspCas13b-GGS-NYTHDF1	dCas13b-GGS-NYTHDF1			Dickinson	Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs. J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.	Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted) cmv d0
	Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)	dCas13b-GGS-NYTHDF2(100-200)			Dickinson	Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs. J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.	Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain) cmv d0
	Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)	dCas13b-GGS-NYTHDF1(100-200)			Dickinson	Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs. J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.	Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain) cmv d0
	Cmv d0 dPspCas13b-GGS-Sun	dCas13b-GGS-Sun			Dickinson	Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs. J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18.	Cmv d0 dPspCas13b-GGS-Sun no-fusion construct cmv d0
	pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag	dPspCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag	dPspCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag	dPguCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag	dPguCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag	dPspCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag	dPspCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag	dPspCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS	dPspCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro
	pHAGE-NLS-dPspCas13b-3xmRuby3-NLS	dPspCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro
	pHAGE-NLS-dPguCas13b-3xmRuby3-NLS	dPguCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro
	pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag	dLwaCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag	dLbaCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag	dRanCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag	dEsCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag	dRfxCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag	dAdmCas13b (Other)			Chen	dCas13 imaging Molecular Cell	overexpression in human cells pHAGE-EF1a-IRES-puro Tag / Fusion Protein flag (N terminal on insert)
	pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE	PguCas13b (Homo sapiens)			Sanjana	Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.	Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac lentiCRISPRv2 Tag / Fusion Protein NLS (C terminal on insert)
	pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE	PguCas13b (Homo sapiens)			Sanjana	Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.	Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac lentiCRISPRv2 Tag / Fusion Protein NES (C terminal on insert)
	pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE	PspCas13b (Homo sapiens)			Sanjana	Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.	Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac lentiCRISPRv2 Tag / Fusion Protein NLS (C terminal on insert)
	pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE	PspCas13b (Homo sapiens)			Sanjana	Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.	Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac lentiCRISPRv2 Tag / Fusion Protein NES (C terminal on insert)
	pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE	CasRx (Homo sapiens)			Sanjana	Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.	Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa lentiCRISPRv2 Tag / Fusion Protein NLS (C terminal on insert)
	pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE	CasRx (Homo sapiens)			Sanjana	Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.	Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa lentiCRISPRv2 Tag / Fusion Protein NES (C terminal on insert)
	pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast	CasRx (Homo sapiens)			Sanjana	Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.	Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus lentiCas9-Blast Tag / Fusion Protein NLS-HA (C terminal on insert)
	pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE	hU6_Rfxcas13d-DR_BsmBI (Homo sapiens)			Sanjana	Massively parallel Cas13 screens reveal principles for guide RNA design. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16.	For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa lentiCRISPRv2
	CARPID BASU-dCasRx	BASU-dCasRx (Homo sapiens)			Yan	CRISPR-assisted detection of RNA-protein interactions in living cells. Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 10.1038/s41592-020-0866-0.	express CARPID BASU-dCasRx fusion protein in mammalian cells pXR001: EF1a-CasRx-2A-EGFP
	CARPID dCasRx-BASU	dCasRx-BASU (Homo sapiens)			Yan	CRISPR-assisted detection of RNA-protein interactions in living cells. Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 10.1038/s41592-020-0866-0.	express CARPID dCasRx-BASU fusion protein in mammalian cells pXR001: EF1a-CasRx-2A-EGFP
	AAV-GFAP-NLS-CasRx-NLS-FLAG	CasRx (Synthetic)	GFAP		Yang	Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. Cell. 2020 Apr 30;181(3):590-603.e16. doi: 10.1016/j.cell.2020.03.024. Epub 2020 Apr 8.	Express CasRx for AAV package pAAV
	PB-CAG-CasRX-P2A-EGFP-BGH PolyA	CasRx (Synthetic)	CAG		Yang	Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2.	Express CasRx PB
	dCas13d-dsRBD	BPNLS-dRfxCas13d-dsRBD-BPNLS-HA	CMV	Blasticidin	Ting	RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting. Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 10.1073/pnas.2006617117.	BPNLS-dRfxCas13d-dsRBD-BPNLS-HA pLX304
	dCas13d-dsRBD-APEX2	TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP	TRE3G	Puromycin	Ting	RNA-protein interaction mapping via MS2- or Cas13-based APEX targeting. Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 10.1073/pnas.2006617117.	TetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP PB-TetON
	pCMV-dCas13-M3nls	dCas13-M3nls (Other)			Liu	Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase. Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.	Targeted m6A RNA methylation in mammalian cells pCMV with pBR322 origin METTL3 IME4, M6A, MT-A70, Spo8, hMETTL3
	pCMV-dCas13-M3M14nes	dCas13-M3M14nes (Other)			Liu	Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase. Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.	Targeted m6A RNA methylation in mammalian cells pCMV with pBR322 origin METTL3 IME4, M6A, MT-A70, Spo8, hMETTL3 METTL14 hMETTL14
	pU6-PspCas13b-gRNA-Actb1216	PspCas13b gRNA targeting Actb 1216 (Synthetic)			Liu	Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase. Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.	PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation pU6 with pBR222 origin
	pCMV-dCas13-inactive M3nls	dCas13-inactive dCas13-inactive M3nls (Other)			Liu	Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase. Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.	Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant pCMV with pBR322 origin METTL3 IME4, M6A, MT-A70, Spo8, hMETTL3
	pCMV-dCas13-inactive M3M14nes	dCas13-inactive M3M14nes (Other)			Liu	Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase. Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6.	Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant pCMV with pBR322 origin METTL3 IME4, M6A, MT-A70, Spo8, hMETTL3 METTL14 hMETTL14

Bacterial

	ID	Plasmid	Gene/Insert	PI	Publication
	70168	pZ003 (LshC2c2 locus)	LshC2c2 full locus (Other)	Zhang	Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
	70170	pZ004 (LseC2c2 locus)	LseC2c2 locus with one spacer in array (Other)	Zhang	Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22.
	79150	pC001 - huLshC2C2-MBP for bacterial expression	C2c2 (Other)	Zhang	C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016 Jun 2. pii: aaf5573.
	79151	pC002 - LshC2C2 locus into pACYC184	LshC2c2 locus (Other)	Zhang	C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016 Jun 2. pii: aaf5573.
	79152	pC003 - LshC2C2 locus into pACYC184 for spacer cloning	LshC2c2 locus (Other)	Zhang	C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 2016 Jun 2. pii: aaf5573.
	83482	p2CT-His-MBP-Lbu_C2c2_WT	Lbu_C2c2_WT (Other)	Doudna	Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016 Sep 26. doi: 10.1038/nature19802.
	83483	p2CT-His-MBP-Lbu_C2c2_R472A_H477A	Lbu_C2c2_R472A_H477A (Other)	Doudna	Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016 Sep 26. doi: 10.1038/nature19802.
	83484	p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A	Lbu_C2c2_R1048A_H1053A (Other)	Doudna	Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016 Sep 26. doi: 10.1038/nature19802.
	83485	p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A	Lbu_C2c2_R472A_H477A_R1048A_H1053A (Other)	Doudna	Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016 Sep 26. doi: 10.1038/nature19802.
	83486	p2CT-His-MBP-Lse_C2c2_WT	Lse_C2c2_WT (Other)	Doudna	Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016 Sep 26. doi: 10.1038/nature19802.
	83487	p2CT-His-MBP-Lsh_C2c2_WT	Lsh_C2c2_WT (Other)	Doudna	Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016 Sep 26. doi: 10.1038/nature19802.
	83494	p2CT-His-MBP-Lbu_C2c2_R1079A	Lbu_C2c2_R1079A (Other)	Doudna	Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 2016 Sep 26. doi: 10.1038/nature19802.
	89898	pBZCas13b	Cas13b (Other)	Zhang	Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28. Mol Cell. 2017 Feb 16;65(4):618-630.e7. doi: 10.1016/j.molcel.2016.12.023. Epub 2017 Jan 5.
	89901	pBZCas13b-HEPN	Cas13b (Other)	Zhang	Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28. Mol Cell. 2017 Feb 16;65(4):618-630.e7. doi: 10.1016/j.molcel.2016.12.023. Epub 2017 Jan 5.
	89906	pPbcas13b	Cas13b (Other)	Zhang	Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28. Mol Cell. 2017 Feb 16;65(4):618-630.e7. doi: 10.1016/j.molcel.2016.12.023. Epub 2017 Jan 5.
	90097	pC013 - Twinstrep-SUMO-huLwCas13a	LwCas13a (Synthetic)	Zhang	Nucleic acid detection with CRISPR-Cas13a/C2c2. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.
	91855	p2CT-His-MBP-Lbu_C2c2_E299A	LbuCas13a (LbuC2c2) E299A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91856	p2CT-His-MBP-Lbu_C2c2_K310A	LbuCas13a (LbuC2c2) K310A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91858	p2CT-His-MBP-Lbu_C2c2_N314A	LbuCas13a (LbuC2c2) N314A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91859	p2CT-His-MBP-Lbu_C2c2_R1072A	LbuCas13a (LbuC2c2) R1072A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91860	p2CT-His-MBP-Lbu_C2c2_D1078A	LbuCas13a (LbuC2c2) D1078A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91861	p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A	LbuCas13a (LbuC2c2) R0179A/K1080A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91862	p2CT-His-MBP-Lbu_C2c2_K1080A	LbuCas13a (LbuC2c2) K1080A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91863	p2CT-His-MBP-Lbu_C2c2_K1082A	LbuCas13a (LbuC2c2) K1082A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91864	p2CT-His-MBP-Lbu_C2c2_K1087A	LbuCas13a (LbuC2c2) K1087A (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91865	p2CT-His-MBP-Lwa_Cas13a_WT	LwaCas13a (LwaC2c2) WT (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91866	p2CT-His-MBP-Lne_Cas13a_WT	LneCas13a (LneC2c2) WT (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91867	p2CT-His-MBP-Lba_Cas13a_WT	LbaCas13a (LbaC2c2) WT (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91868	p2CT-His-MBP-Ere_Cas13a_WT	EreCas13a (EreC2c2) WT (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91869	p2CT-His-MBP-Cam_Cas13a_WT	CamCas13a (CamC2c2) WT (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91870	p2CT-His-MBP-Rca_Cas13a_WT	RcaCas13a (RcaC2c2) WT (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91871	p2CT-His-MBP-Hhe_Cas13a_WT	HheCas13a (HheC2c2) WT (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91872	p2CT-His-MBP-Ppr_Cas13a_WT	PprCas13a (PprC2c2) WT (Other)	Doudna	RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008.
	91900	pC009 LshCas13a locus with targeting spacer	LshCas13a locus with targeting spacer (Other)	Zhang	Nucleic acid detection with CRISPR-Cas13a/C2c2. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.
	91901	pC010 LshCas13a locus with nontargeting spacer	LshCas13a locus with non-targeting spacer (Other)	Zhang	Nucleic acid detection with CRISPR-Cas13a/C2c2. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.
	91903	pC011 LwCas13a locus with targeting spacer	LwCas13a with targeting spacer (Other)	Zhang	Nucleic acid detection with CRISPR-Cas13a/C2c2. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.
	91904	pC012 LwCas13a locus with nontargeting spacer	LwCas13a with non-targeting spacer (Other)	Zhang	Nucleic acid detection with CRISPR-Cas13a/C2c2. Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321.
	91908	pC018 - LshCas13a from Leptotrichia shahii	LshCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91909	pC019 - LwCas13a from Leptotrichia wadei	LwCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91910	pC020 - LseCas13a from Listeria seeligeri	LseCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91911	pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020	LbmCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91912	pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179	LbnCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91913	pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710	CaCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91914	pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847	CgCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91915	pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847	Cg2Cas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91916	pC026 - PpCas13a from Paludibacter propionicigenes WB4	PpCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91917	pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317	LweCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91918	pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635	LbfCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91919	pC029 - Lw2Cas13a from Leptotrichia wadei F0279	Lw2Cas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91920	pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003	RcsCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91921	pC031 - RcrCas13a from Rhodobacter capsulatus R121	RcrCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	91922	pC032 - RcdCas13a from Rhodobacter capsulatus DE442	RcdCas13a and guide expression cassette (Other)	Zhang	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	100817	pDuBir-Lbu-dCas13a-avitag	Lbu_Cas13a_R472A_H477A_R1048A_H1053A (Other)	O'Connell	RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a. Cell Rep. 2018 Jul 24;24(4):1025-1036. doi: 10.1016/j.celrep.2018.06.105.
	108303	pET28a-MH6-EsCas13d	EsCas13d (Synthetic)	Biotechnologies	Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein. Mol Cell. 2018 Mar 9. pii: S1097-2765(18)30173-4. doi: 10.1016/j.molcel.2018.02.028.
	108304	pET28a-MH6-RspCas13d_RspCasWYL1	RspCas13d (Synthetic), RspWYL1 (Synthetic)	Biotechnologies	Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein. Mol Cell. 2018 Mar 9. pii: S1097-2765(18)30173-4. doi: 10.1016/j.molcel.2018.02.028.
	108305	pET28a-MH6-RspCas13d	RspCas13d (Synthetic)	Biotechnologies	Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein. Mol Cell. 2018 Mar 9. pii: S1097-2765(18)30173-4. doi: 10.1016/j.molcel.2018.02.028.
	115207	pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI	BzoCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115208	pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI	PinCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115209	pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI	PbuCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115210	pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI	AspCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115211	pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI	PsmCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115213	pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI	RanCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115214	pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI	PauCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115216	pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI	Pin2Cas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115218	pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI	PguCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115219	pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI	PspCas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115220	pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI	Pin3Cas13 (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	115267	pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI	LbuCas13a (Other)	Zhang	Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15.
	131012	pLsCas13aGG	direct repeat of LsCas13a, GFP expression cassette	Beisel	Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis. Nat Commun. 2019 Jul 3;10(1):2948. doi: 10.1038/s41467-019-10747-3.
	141322	pET-28b-RfxCas13d-His	Rfx-Cas13d (Other)	Moreno-Mateos	CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos Developmental Cell. 2020.
	164862	pBzCas13b	Bergeyella zoohelcum Cas13b (Other)	Beisel	Rapidly characterizing CRISPR-Cas13 nucleases using cell-free transcription-translation systems (unpublished)

Plant

	ID	Plasmid	Gene/Insert	PI	Publication
	107691	pJJB296	Cas13a (Other)	Voytas	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	107694	pJJB302	Cas13a (Other)	Voytas	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	107699	pJJB308		Voytas	RNA targeting with CRISPR-Cas13. Nature. 2017 Oct 4. doi: 10.1038/nature24049.
	118963	puc19-pCas13a	Cas13a (Arabidopsis thaliana)	Mahfouz	RNA virus interference via CRISPR/Cas13a system in plants. Genome Biol. 2018 Jan 4;19(1):1. doi: 10.1186/s13059-017-1381-1.
	160629	pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)	AtUBQ10-:LbCpf1(Cas12a):Tnos (Other)	Orzaez	Orzaez lab plasmids (unpublished)