| pMJ806 | Cas9 | T7 |
Doudna
| A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity.
Science. 2012 Jun 28.
|
pEC-K-MBP
|
| pCas9 | tracr/Cas9 | |
Marraffini
| RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
|
Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide
pACYC184
|
| pwtCas9-bacteria | wild-type Cas9 | pLtetO-1 |
Qi
| Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
|
aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown
pUC19
|
| pET-28b-Cas9-His | Cas9 (Other) | |
Schier
| Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
PLoS One. 2014 May 29;9(5):e98186. doi: 10.1371/journal.pone.0098186. eCollection 2014.
|
For in vitro expression and purification of Cas9 protein
pET-28b
|
| DS-SPcas | Cas9 (Other), tracrRNA precursor (Other) | proC, tracrRNA promoter |
Church
| Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
|
Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin
cloDF13-aadA
|
| DS-NMcas | Cas9 (Other), NM tracrRNA (Other) | proC, NM tracrRNA promoter |
Church
| Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
|
Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin
cloDF13-aadA
|
| DS-ST1cas | Cas9 (Other), ST1 tracrRNA (Other) | proC |
Church
| Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
|
Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin
cloDF13-aadA
|
| pET28a/Cas9-Cys | Cas9-Cys (Other) | T7 Promoter |
Kim
| Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA.
Genome Res. 2014 Apr 2.
|
Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
pET28a
|
| pCRISPomyces-1 | sSpCas9 (Synthetic), tracrRNA (Other), crRNA cassette (Synthetic) | rpsLp(XC)-BbsI, rpsLp(CF), gapdhp(EL) |
Zhao
| High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System.
ACS Synth Biol. 2014 Dec 8.
|
Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
pKC1139
|
| pCRISPomyces-2 | sSpCas9 (Synthetic), gRNA cassette (Synthetic) | rpsL(XC)-BbsI, gapdhp(EL) |
Zhao
| High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System.
ACS Synth Biol. 2014 Dec 8.
|
Streptomyces expression of codon-optimized Cas9 and custom gRNA
pKC1139
|
| pCas | cas9 (Other) | native cas9 promoter |
Yang
| Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.
Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
|
Constitutive expression of cas9 and inducible expression of lambda RED and sgR
pKD46
|
| pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis | (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis (Other) | T7 |
Liu
| Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo.
Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30.
|
Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
pET29
|
| pET-Cas9-6xHis | Cas9_6xHis (Other) | T7 |
Liu
| Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo.
Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30.
|
Expression of Cas9-6xHis in bacterial cells
pET29
|
| pTrex-b-NLS-hSpCas9 | NLS-hSpCas9 | Ribo-HX1 |
Tarleton
| CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi.
MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14.
|
Expression of Cas9 in T. cruzi
pTrex-b
|
| pKCcas9dO | Scocas9 (Other), sgRNA (), act-orf4 homology arm | ptipA, j23119 |
Jiang
| One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces.
Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):231-43. doi: 10.1093/abbs/gmv007. Epub 2015 Mar 3.
|
High efficiency Streptomyces genome editing by CRISPR/Cas9 system
pKC1139
|
| pCas9-CR4 | Cas9 nuclease | |
Prather
| The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.
Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
|
Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR
pdCas9-bacteria
|
| SP-Cas9 | SP-CAS9 (Other) | T7 |
Geijsen
| Efficient Intracellular Delivery of Native Proteins.
Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028.
|
Expresses SP-Cas9 protein in bacterial cells
pET-15
|
| pCMK | Cas9 (Synthetic) | |
Rodrigue
|
Bacterial constitutive gRNA expression plasmid
(unpublished)
|
Bacterial constitutive S. pyogenes Cas9 expression plasmid
pCMK
|
| pET-Cas9-NLS-6xHis | Cas9-NLS-6xHis (Other) | T7 |
Liu
| Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo.
Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30.
|
Expression of Cas9-NLS-6xHis in bacterial cells
pET29
|
| pET-NLS-Cas9-6xHis | NLS-Cas9-6xHis (Other) | T7 |
Liu
| Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo.
Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30.
|
Expression of NLS-Cas9-6xHis in bacterial cells
pET29
|
| pLPhygCAS9 | Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene) (Other) | |
Matlashewski
| CRISPR-Cas9-Mediated Genome Editing in Leishmania donovani.
MBio. 2015 Jul 21;6(4). pii: e00861-15. doi: 10.1128/mBio.00861-15.
|
Expresses CAS9 in Leishmania
pLPHyg2
|
| pYTK036 | Cas9 (Other) | |
Dueber
| A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly.
ACS Synth Biol. 2015 Apr 14.
|
Encodes Cas9 as a Type 3 part to be used in the Dueber YTK system
pYTK001
|
| BPK764 | mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA (Other) | T7 (x2) |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
pACYCDuet-1
Tags
/ Fusion Proteins- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
|
| MSP1673 | mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA (Other) | T7 (x2) |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA
pACYCDuet-1
Tag
/ Fusion Protein- NLS (C terminal on insert)
|
| BPK2101 | mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag, and SaCas9 gRNA (Other) | T7 (x2) |
Joung
| Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
Nature. 2015 Jun 22. doi: 10.1038/nature14592.
|
Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
pACYCDuet-1
Tags
/ Fusion Proteins- NLS (C terminal on insert)
- 3x FLAG (C terminal on insert)
|
| pHO4d-Cas9 | Cas9 (Other) | T7 |
Nonet
| Landscape of target:guide homology effects on Cas9-mediated cleavage.
Nucleic Acids Res. 2014 Dec 16;42(22):13778-87. doi: 10.1093/nar/gku1102. Epub 2014 Nov 15.
|
Cas9nlsHis6 Bacterial Expression construct
pHO4D
|
| pMJ915 | cas9 (Other) | T7 |
Doudna
| Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery.
Elife. 2014 Dec 15;3:e04766. doi: 10.7554/eLife.04766.
|
Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS
pML-2CT
|
| MSP2283 | mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag (Other), SaCas9 sgRNA (+84) | T7, T7 |
Joung
| Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition.
Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
|
Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
pACYCDuet-1
|
| MSP2253 | mammalian codon-optimized KKH variant Staphylococcus aureus Cas9(E782K/N968K/R1015H)-NLS-3xFlag (Other), SaCas9 sgRNA (+84) | T7, T7 |
Joung
| Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition.
Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
|
Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
pACYCDuet-1
|
| MSP2266 | mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag (Other), SaCas9 sgRNA (+84) | T7, T7 |
Joung
| Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition.
Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
|
Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
pACYCDuet-1
|
| MSP2292 | mammalian codon-optimized KKH variant Staphylococcus aureus Cas9(E782K/N968K/R1015H)-NLS-3xFlag, and SaCas9 sgRNA(84) (Other), SaCas9 sgRNA (+84) | T7, T7 |
Joung
| Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition.
Nat Biotechnol. 2015 Nov 2. doi: 10.1038/nbt.3404.
|
Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
pACYCDuet-1
|
| pCas9_sgRNA_0 | U6 promoter (Other), cas9 (Synthetic), tnos terminator (Other) | U6 from Ustilago maydis, synthetic otef promoter (Spellig et al., 1996, Mol. Gen. Genet. 252) |
Kahmann
| Genome editing in Ustilago maydis using the CRISPR-Cas system.
Fungal Genet Biol. 2015 Sep 11. pii: S1087-1845(15)30025-6. doi: 10.1016/j.fgb.2015.09.001.
|
expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
pNEBUC
|
| pMCSG7-Wt-NmeCas9 | pMCSG7-WT-NmeCas9 (Other) | |
Sontheimer
| DNase H Activity of Neisseria meningitidis Cas9.
Mol Cell. 2015 Oct 15;60(2):242-55. doi: 10.1016/j.molcel.2015.09.020.
|
bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site
pMCSG7
|
| pCas9cur | Cas9 (Other), Plasmid curing system | pBAD |
Chen
| Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.
Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30.
|
Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
pSC101ts
|
| pREDCas9 | Cas9 (Other), lambda red genes, plasmid curing system | pBAD |
Chen
| Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.
Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30.
|
Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
pSC101ts
|
| pCAS1yl | Cas9 (Other), sgRNA (Synthetic) | unknown |
Yang
| Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system.
J Ind Microbiol Biotechnol. 2016 Aug;43(8):1085-93. doi: 10.1007/s10295-016-1789-8. Epub 2016 Jun 27.
|
Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells
pMCSCen1
|
| pMA7CR_2.0 | cas9 (Other), lambda beta (Other), dam (Other), recX (Other) | pTet, pAra, pAra, pTet |
Nielsen
| CRMAGE: CRISPR Optimized MAGE Recombineering.
Sci Rep. 2016 Jan 22;6:19452. doi: 10.1038/srep19452.
|
pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX
pBAD24
|
| pDEST-hisMBP-AsCpf1-EC | AsCpf1 (Synthetic) | tac promoter |
Kim
| Targeted mutagenesis in mice by electroporation of Cpf1 ribonucleoproteins.
Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3596.
|
Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)
pDEST-hisMBP
|
| pMAL-his-LbCpf1-EC | LbCpf1 (Synthetic) | tac promoter |
Kim
| Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells.
Nat Biotechnol. 2016 Jun 6. doi: 10.1038/nbt.3609.
|
Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)
pMAL-c5X
|
| pCas9-CAT | Cas9 (Other), Chloramphenicol acetyltransferase | TgTUB1, TgTUB1 |
Lourido
| A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.
Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
|
Encodes Cas9 and chloramphenicol acetyltransferase (CAT)
pU6-Universal
|
| pU6-Decoy | Decoy sgRNA | U6 |
Lourido
| A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.
Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
|
Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii
pU6-Universal
|
| bbCas9pluspAAA | Cas9 (Other) | T7 |
Huijbers
| Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes.
Methods Mol Biol. 2017;1642:21-35. doi: 10.1007/978-1-4939-7169-5_2.
|
The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA
pUC
|
| pEC-K-CBP_CjeCas9 | CjeCas9 (Other) | |
Doudna
| Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes.
Mol Cell. 2015 Nov 5;60(3):398-407. doi: 10.1016/j.molcel.2015.10.030.
|
Expresses Campylobacter jejuni Cas9 (Cje Cas9).
pEC-K
|
| p2CT-Cdi | CdiCas9 | |
Doudna
| Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes.
Mol Cell. 2015 Nov 5;60(3):398-407. doi: 10.1016/j.molcel.2015.10.030.
|
Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)
PET
|
| pLdCN | gRNA and Cas9 (Other) | L. donovani ribosome RNA promoter |
Matlashewski
| Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms.
mSphere. 2017 Jan 18;2(1). pii: e00340-16. doi: 10.1128/mSphere.00340-16. eCollection 2017 Jan-Feb.
|
Express gRNA and Cas9 in Leishmania with Neomycin resistance
pSP72
|
| pLdCH | gRNA and Cas9 (Other) | L. donovani ribosome RNA promoter |
Matlashewski
| Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms.
mSphere. 2017 Jan 18;2(1). pii: e00340-16. doi: 10.1128/mSphere.00340-16. eCollection 2017 Jan-Feb.
|
Express gRNA and Cas9 in Leishmania with Hygromycin resistance
pSP72
|
| pJYS3_ΔcrtYf | Cpf1 (Other), crRNA of crtYf (Synthetic) | |
Yang
| CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
|
Constitutive transcription of FnCpf1 and crRNA of crtYf
pXMJ19
|
| pJYS1Ptac | Cpf1 (Other), recT (Other) | tac, unknown |
Yang
| CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
|
Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter
pXMJ19
|
| pJYS1Peftu | Cpf1 (Other), recT (Other) | etfu, unknown |
Yang
| CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
|
Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter
pXMJ19
|
| pX2-Cas9 | Cas9 (Other) | pBAD |
Gill
|
pX2-Cas9
(unpublished)
|
Arabinose inducible Cas9 in a broad host range backbone.
pBTBX-2
|
| pSHS212 | Cas9 (Other) | T7 |
Doudna
| Conformational control of DNA target cleavage by CRISPR-Cas9.
Nature. 2015 Nov 5;527(7576):110-3. doi: 10.1038/nature15544. Epub 2015 Oct 28.
|
Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid
pCT10
|
| pSHS293 | Cas9 (Other) | T7 |
Doudna
| Conformational control of DNA target cleavage by CRISPR-Cas9.
Nature. 2015 Nov 5;527(7576):110-3. doi: 10.1038/nature15544. Epub 2015 Oct 28.
|
D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct
pCT10
|
| pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant | Cas9 (Other) | T7 |
Doudna
| Conformational control of DNA target cleavage by CRISPR-Cas9.
Nature. 2015 Nov 5;527(7576):110-3. doi: 10.1038/nature15544. Epub 2015 Oct 28.
|
S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct
pCT10
|
| pSHS248 | Cas9 (Other) | T7 |
Doudna
| Conformational control of DNA target cleavage by CRISPR-Cas9.
Nature. 2015 Nov 5;527(7576):110-3. doi: 10.1038/nature15544. Epub 2015 Oct 28.
|
S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct
pCT10
|
| pET-MBP-Geo_st | GeoCas9 (Other) | |
Doudna
|
GeoCas9 Plasmids
(unpublished)
|
Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
pET
|
| pET-MBP-NLS-Geo_st | GeoCas9 (Other) | |
Doudna
|
GeoCas9 Plasmids
(unpublished)
|
Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
pET
|
| pFC330 | Cas9 (Synthetic), pyrG (Other) | Aspergillus nidulans tef1 promoter, native promoter |
Mortensen
| A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi.
PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015.
|
AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker
custom
|
| pFC331 | Cas9 (Synthetic), argB (Other) | Aspergillus nidulans tef1 promoter, native promoter |
Mortensen
| A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi.
PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015.
|
AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker
custom
|
| pFC333 | Cas9 (Synthetic), ble (bleomycin resistance marker) (Other) | Aspergillus nidulans tef1 promoter, Aspergillus nidulans trpC promoter |
Mortensen
| A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi.
PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015.
|
AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
custom
|
| pFC332 | Cas9 (Synthetic), hph (hygromycin resistance marker) (Other) | Aspergillus nidulans tef1 promoter, Aspergillus nidulans trpC promoter |
Mortensen
| A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi.
PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015.
|
AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker
custom
|
| pMJ915v2+Nterm Spe1 +Cterm Age1 site | | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| 1xNLS-pMJ915v2 | Cas9 | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| 2xNLS-pMJ915v2 | Cas9, T7 | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| 4xNLS-pMJ915v2 | Cas9, T7 | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| pMJ915v2 + sfGFP | Cas9, T7 | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| 1xNLS-pMJ915v2-sfGFP | Cas9, T7 | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| 2xNLS-pMJ915v2-sfGFP | Cas9, T7 | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| 4xNLS-pMJ915v2-sfGFP | Cas9, T7 | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| 7xNLS-pMJ915v2-sfGFP | Cas9, T7 | |
Doudna
| Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3806.
|
For expression of modified SpyCas9 in e.coli.
pMJ915
|
| pET-CjCas9 | CjCas9 (Other) | T7 |
Kim
| In vivo genome editing with a small Cas9 orthologue derived from Campylobacter jejuni.
Nat Commun. 2017 Feb 21;8:14500. doi: 10.1038/ncomms14500.
|
Expression of CjCas9 with His tag in E.coli
pET28b
|
| pEM-Cas9HF1 | Cas9HF1 (Other) | pTet |
Lynch
| Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.
ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174.
|
Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
pCas9-CR4
|
| pEM-Cas9HF1-recA56 | Cas9HF1 (Other), recA56 (Other) | pTet, proD |
Lynch
| Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.
ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174.
|
Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.
pEM-Cas9HF1
|
| 6-His-MBP-TEV-FnCpf1 | FnCpf1 (humanized) (Other) | T7 |
Zhang
| Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038.
|
Bacterial expression plasmid for protein purification
pET-28
|
| 6His-MBP-TEV-huAsCpf1 | huAsCpf1 (Other) | |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Bacterial expression plasmid for protein purification
pET-28
|
| 6His-MBP-TEV-huLbCpf1 | huLbCpf1 (Other) | |
Zhang
| Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 2016 Dec 5.
|
Bacterial expression plasmid for protein purification
pET-28
|
| pMST665_BB3_L 23_gRNAempty_cas9_BbsI | Cas9 | |
Sauer
| An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger.
Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004.
|
BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9
BB3_AMA_2.8_pUC-ORI_L_AC_hph
|
| pFREE | Cas9, gRNA array (Synthetic) | ptet, pRhamBAD |
Norholm
| A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z.
|
Allows inducible expression of gRNAs and Cas9 for plasmid curing.
pMAZ-SK
|
| pFREE_Amp | Cas9, gRNA array (Synthetic) | ptet, pRhamBAD |
Norholm
| A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z.
|
Allows inducible expression of gRNAs and Cas9 for plasmid curing.
pMAZ-SK
|
| pFREE_Cm | Cas9, gRNA array (Synthetic) | ptet, pRhamBAD |
Norholm
| A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z.
|
Allows inducible expression of gRNAs and Cas9 for plasmid curing.
pMAZ-SK
|
| pFREE_Zeo | Cas9, gRNA array (Synthetic) | ptet, pRhamBAD |
Norholm
| A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z.
|
Allows inducible expression of gRNAs and Cas9 for plasmid curing.
pMAZ-SK
|
| pFREE-RK2 | Cas9, gRNA array (Synthetic) | ptet, pRhamBAD |
Norholm
| A versatile one-step CRISPR-Cas9 based approach to plasmid-curing.
Microb Cell Fact. 2017 Aug 2;16(1):135. doi: 10.1186/s12934-017-0748-z.
|
Allows inducible expression of gRNAs and Cas9 for plasmid curing as well as self-curing via a temperature sensitive RK2 replicon.
pMAZ-SK
|
| pLQ-Pxyl/tet-cas9-Pj23119-sgRNA | Cas9-Pxyltet-sgRNA-pj23119 (Other) | |
Yang
| CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus.
Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):764-770. doi: 10.1093/abbs/gmx074.
|
CRISPR-Cas9 based efficient genome editing in S. aureus
unknown
|
| pET28a-Cas9-His | NLS-Cas9-NLS (Other) | T7 |
Gao
| Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes.
Nat Commun. 2017 Jan 18;8:14261. doi: 10.1038/ncomms14261.
|
Purification of Cas9 protein
pET28a (+)
|
| p46Cpf1-OP2 | FnCpf1 (Other) | araBAD |
Wu
|
Qiong Wu CRISPR plasmids
(unpublished)
|
Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
pKD46
|
| pJWV102-wtcas9sp | cas9sp (Synthetic) | PczcD |
Kjos
| Chromosome segregation drives division site selection in Streptococcus pneumoniae.
Proc Natl Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 10.1073/pnas.1620608114.
|
Plasmid containing wild-type cas9sp
pJWV102
|
| pThermoCas9_ctrl | Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain (Other), ThermoCas9 single guide RNA expressing module (Other) | B. smithii xylL promoter, B. coagulans DSM 1 pta promoter |
van der Oost
| Characterizing a thermostable Cas9 for bacterial genome editing and silencing.
Nat Commun. 2017 Nov 21;8(1):1647. doi: 10.1038/s41467-017-01591-4.
|
Expresses ThermoCas9 and its sgRNA module
pNW33n
|
| SpyCas9(WT) | SpyCas9 (Synthetic) | |
Huang
| Structural basis of CRISPR-SpyCas9 inhibition by an anti-CRISPR protein.
Nature. 2017 Jun 15;546(7658):436-439. doi: 10.1038/nature22377. Epub 2017 Apr 27.
|
Express Streptococcus pyogenes Cas9
pGEX-6P-1
Tag
/ Fusion Protein- GST (N terminal on backbone)
|
| p6XHis_NLS-SaCas9 | CRISPR-associated protein Cas9/Csn1 [Staphylococcus aureus subsp. aureus] (Other) | T7lac |
Tarleton
| Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins.
MBio. 2017 Nov 7;8(6). pii: e01788-17. doi: 10.1128/mBio.01788-17.
|
Express SaCas9 in bacteria with a 6xHis tag for purification
pET-32 EK/LIC
|
| pSHS207 - Bacterial expression plasmid for WT SpCas9 | SpCas9 (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for WT SpCas9
pCT10
|
| pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant | SpCas9 variant C80S/C574S/E60C/D273C (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9, REC2 FRET variant
pCT10
|
| pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant | SpCas9 variant C80S/C574S/S701C/S960C (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9, REC3 FRET variant
pCT10
|
| pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant | SpCas9 variant M1–N497,GGS,V713–D1368 (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9∆REC3 variant
pCT10
|
| pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant | SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368 (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant
pCT10
|
| pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant | SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368 (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
pCT10
|
| pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain | SpCas9 variant K506–Q712 (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 REC3 domain
pCT10
|
| pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant | SpCas9 variant Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 + Q926A variant
pCT10
|
| pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant | SpCas9 variant N497A/R661A/Q695A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant
pCT10
|
| pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant | SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
pCT10
|
| pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant | SpCas9 variant N497A/R661A/Q695A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9-HF1 variant
pCT10
|
| pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant | SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant
pCT10
|
| pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant | SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
pCT10
|
| pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant | SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
pCT10
|
| pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant | SpCas9 variant K855A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 + K855A variant
pCT10
|
| pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant | SpCas9 variant C80S/C574S/S355C/S867C/K855A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
pCT10
|
| pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant | SpCas9 variant K848A/K1003A/R1060A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
pCT10
|
| pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant | SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
pCT10
|
| pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant | SpCas9 variant N692A/M694A/Q695A/H698A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant
pCT10
|
| pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant | SpCas9 variant N692A/M694A/Q695A/H698A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
pCT10
|
| pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant | SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
pCT10
|
| pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant | SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
pCT10
|
| pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant | SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
pCT10
|
| pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant | SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
pCT10
|
| pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant | SpCas9 variant G582A/V583A/E584A/D585A/N588A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 2 variant
pCT10
|
| pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant | SpCas9 variant T657A/G658A/W659A/R661A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
pCT10
|
| pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant | SpCas9 variant F491A/M495A/T496A/N497A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
pCT10
|
| pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant | SpCas9 variant K918A/V922A/R925A/Q926A (Other) | T7 |
Doudna
| Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
|
Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant
pCT10
|
| pEM-Cas9noSsrA | Cas9 (Other) | pTet |
Lynch
| Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.
ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174.
|
Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.
pCas9-CR4
|
| pEM-Cas9HF1-indRecA56 | Cas9HF1 (Other), recA56 | pTet, pTet |
Lynch
| Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.
ACS Synth Biol. 2017 Oct 2. doi: 10.1021/acssynbio.7b00174.
|
Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.
pEM-Cas9HF1
|
| AsCpf1-2NLS | AsCpf1-6xHis-MBP-TEV (Synthetic) | T7 |
Doudna
| CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing.
Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
|
bacterial expression of AsCpf1-2NLS
pET
|
| LbCpf1-2NLS | LbCpf1 (Synthetic) | T7 |
Doudna
| CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing.
Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2.
|
bacterial expression of LbCpf1-2NLS
pET
|
| pSBY1_FnCpf1cg | FnCpf1 (Other) | hsp60 |
Yang
| A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis.
Biotechnol J. 2018 Sep;13(9):e1700588. doi: 10.1002/biot.201700588. Epub 2018 Aug 6.
|
CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1
pMV261
|
| pJK02 | Upstream & Downstream pyrE deletion region (Other), tetR, Cas9 (Other), gRNA | |
Sorg
| Using CRISPR-Cas9-mediated genome editing to generate C. difficile mutants defective in selenoproteins synthesis.
Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.1038/s41598-017-15236-5.
|
Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT
pMTL84151
|
| pKM126 | Upstream & Downstream pyrE deletion region (Other), tetR, Cas9 (Other), gRNA | |
Sorg
| Using CRISPR-Cas9-mediated genome editing to generate C. difficile mutants defective in selenoproteins synthesis.
Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.1038/s41598-017-15236-5.
|
Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT
pJS116
|
| pCas9 | cas9 (Other), Lambda red genes (Other) | J23105, araBAD |
Pfleger
| Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.
J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
|
Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ
pSEVA224
|
| p2T-CAG-KKHSaCas9-BlastR | KKH SaCas9 | Chicken ß-Actin |
Sherwood
| Predictable and precise template-free CRISPR editing of pathogenic variants.
Nature. 2018 Nov;563(7733):646-651. doi: 10.1038/s41586-018-0686-x. Epub 2018 Nov 7.
|
Confers constitutive expression of KKH SaCas9.
p2T-CAG-MCS-BlastR
NEWENTRY
|
| pCAS9counter | Cas9, sgRNA | prab17, prab17 |
Salipante
| Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection.
MBio. 2018 Feb 20;9(1). pii: mBio.00067-18. doi: 10.1128/mBio.00067-18.
|
Expresses CAS9 and sgRNA for targeted counterselection in S. aureus
pCN-50
|
| pET28b-NmCas9-Flag | CRISPR-associated endonuclease Cas9 (Other) | T7 |
Zhang
| Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis.
Mol Cell. 2018 Mar 1;69(5):906-914.e4. doi: 10.1016/j.molcel.2018.01.025. Epub 2018 Feb 15.
|
Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression
pET28b
|
| pxCas9CR4 | TetR and xCas9 (Synthetic) | |
Reisch
|
pCas9CR4 with point mutations from Cas9X for NG PAM sites
(unpublished)
|
PCas9CR4 with the xCas9 mutations allowing NG PAM sites
p15A origin
|
| pRPaCas9 | Cas9 (Other) | |
Horn
| Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes.
Sci Rep. 2018 May 21;8(1):7960. doi: 10.1038/s41598-018-26303-w.
|
Expresses Cas9 in T. brucei (2T1) cells
pUC
|
| pCas9-J23109 | Cas9 (Other) | |
Zhang
| Improved sgRNA design in bacteria via genome-wide activity profiling.
Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572.
|
Plasmid constitutively expressing Cas9 protein
N.A.
|
| peSpCas9-J23109 | eSpCas9 (Other) | |
Zhang
| Improved sgRNA design in bacteria via genome-wide activity profiling.
Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572.
|
Plasmid constitutively expressing eSpCas9 protein
N.A.
|
| pCasPA | | |
Ji
| CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species.
iScience. 2018 Aug 31;6:222-231. doi: 10.1016/j.isci.2018.07.024. Epub 2018 Aug 1.
|
Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa
unknown
|
| pNS20-SpCas9-SNAP | Cas9 (Other) | |
Schwank
| Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair.
Elife. 2018 May 29;7. pii: 33761. doi: 10.7554/eLife.33761.
|
Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9
pET His6 MBP N10 TEV LIC cloning vector (2C-T)
|
| pET-21a_3xNLS_SpCas9_protein_expression | 3xNLS_SpCas9 (Synthetic) | T7 |
Wolfe
| Highly efficient therapeutic gene editing of human hematopoietic stem cells.
Nat Med. 2019 May;25(5):776-783. doi: 10.1038/s41591-019-0401-y. Epub 2019 Mar 25.
|
Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone
pET-21 a
|
| pET-21a_2xNLS_LbCpf1_protein_expression | 2xNLS_LbCpf1 (Synthetic) | T7 |
Wolfe
| Editing aberrant splice sites efficiently restores beta-globin expression in beta-thalassemia.
Blood. 2019 Jan 31. pii: blood-2019-01-895094. doi: 10.1182/blood-2019-01-895094.
|
Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone
pET-21 a
|
| pJL1-SpCas9 | S. pyogenes Cas9 (Other) | T7 |
Jewett
| BioBits Health: Classroom Activities Exploring Engineering, Biology, and Human Health with Fluorescent Readouts.
ACS Synth Biol. 2019 May 7. doi: 10.1021/acssynbio.8b00381.
|
In vitro expression of S. pyogenes Cas9 from the T7 promoter
pJL1
|
| pCasKP-apr | Cas9 (Other) | |
Ji
| Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase.
Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
|
Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae
Unknown
|
| pCasKP-hph | Cas9 (Other) | |
Ji
| Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase.
Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
|
Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae
Unknown
|
| pHSB04X | Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019 (Other) | |
Yang
| Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus.
Biotechnol J. 2019 Jul;14(7):e1800690. doi: 10.1002/biot.201800690. Epub 2019 May 20.
|
Genome editing for Lactobacillus brevis ATCC367
pCas
|
| pHSP02 | Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537 (Other) | |
Yang
| Development of a RecE/T-Assisted CRISPR-Cas9 Toolbox for Lactobacillus.
Biotechnol J. 2019 Jul;14(7):e1800690. doi: 10.1002/biot.201800690. Epub 2019 May 20.
|
Genome editing for Lactobacillus plantarum WCSF1
pCas
|
| p15A_SpyCas9_CmR | Cas9 from S. pyogenes | |
Noireaux
|
An educational module to explore CRISPR technologies with a cell-free transcription-translation system
(unpublished)
|
Expresses S. pyogenes Cas9
p15A, Chloramphenicol resistance
NEWENTRY
|
| pCas9-J23113 | Cas9 (Other) | |
Zhang
| Improved sgRNA design in bacteria via genome-wide activity profiling.
Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572.
|
Expression of Cas9 with a weak promoter
p15A origin
|
| Nme2Cas9_pMCSG7 | Nme2Cas9 (Other) | T7 |
Sontheimer
| A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing.
Mol Cell. 2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 10.1016/j.molcel.2018.12.003.
|
Nme2Cas9 bacterial expression plasmid
pMCSG7
|
| NmeCas9_3XNLS_pMCSG7 | NmeCas9 (Other) | |
Sontheimer
|
NmeCas9 is an intrinsically high-fidelity genome editing platform
(unpublished)
|
Bacterial expression of wtNmeCas9 with 3X NLS
pMCSG7
|
| pCas9CR4-VQR | SpCas9-VQR (Synthetic) | pTet |
Reisch
|
pCas9CR4 variants for increased targeting space
(unpublished)
|
pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
pdCas9-bacteria
|
| pCas9CR4-NG | SpCas9-NG (Synthetic) | pTet |
Reisch
|
pCas9CR4 variants for increased targeting space
(unpublished)
|
pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
pdCas9-bacteria
|
| pCas9CR5 | SpCas9 (Synthetic) | pTet |
Reisch
|
pCas9CR4 variants for increased targeting space
(unpublished)
|
pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
pdCas9-bacteria
|
| pCas9CR5-NG | SpCas9-NG (Synthetic) | pTet |
Reisch
|
pCas9CR4 variants for increased targeting space
(unpublished)
|
pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
pdCas9-bacteria
|
| pJZ002 | | |
Moore
| Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 10.1016/j.chembiol.2019.02.004.
|
CRISPR-cas9 targeting in E. coli with ampicillin resistance
N/A
|
| pSU-araC-Cas9 | Cas9 (Other) | araBAD |
Yang
| Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system.
Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14.
|
Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system
p15A
|
| pET-21a_2xNLS_FnCpf1_MBP_protein_expression | 2xNLS_FnCpf1 (Synthetic) | T7 |
Wolfe
| Enhanced Cas12a editing in mammalian cells and zebrafish.
Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184.
|
Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone
pET-21 a
|
| pUC-fFuCas9-HTBNLS-hph | Cas9 and hygromycin resist gene (Other) | gpdA;trpC |
Ji
| CRISPR/Cas9-Based Genome Editing in the Filamentous Fungus Fusarium fujikuroi and Its Application in Strain Engineering for Gibberellic Acid Production.
ACS Synth Biol. 2019 Feb 15;8(2):445-454. doi: 10.1021/acssynbio.8b00478. Epub 2019 Jan 23.
|
Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system
pUC57
|
| pEJS1026-pMCSG7-HpaCas9 | HpaCas9 (Other) | |
Sontheimer
| Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins.
MBio. 2018 Dec 4;9(6). pii: mBio.02321-18. doi: 10.1128/mBio.02321-18.
|
Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells
pMCSG7
|
| pEJS1027-pMCSG7-SmuCas9 | SmuCas9 (Other) | |
Sontheimer
| Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins.
MBio. 2018 Dec 4;9(6). pii: mBio.02321-18. doi: 10.1128/mBio.02321-18.
|
Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells
pMCSG7
|
| pCasAb-apr | | |
Ji
| A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR.
Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 10.1016/j.chembiol.2019.09.003.
|
Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii
pMMB67EH
|
| pCpf1 | | |
Zhang
| Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120.
ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
|
Bacterial genome editing plasmid with kanamycin resistance marker
Unknown
|
| pCpf1-sp | | |
Zhang
| Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120.
ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
|
Bacterial genome editing plasmid with spectinomycin resistance marker
Unknown
|
| pCpf1b | | |
Zhang
| Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120.
ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
|
Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker
Unknown
|
| pCpf1b-sp | | |
Zhang
| Expanding the potential of CRISPR-Cpf1 based genome editing technology in the cyanobacterium Anabaena PCC 7120.
ACS Synth Biol. 2018 Dec 10. doi: 10.1021/acssynbio.8b00437.
|
Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker
Unknown
|
| pLdSaCN | gRNA and SaCas9 (Other) | |
Matlashewski
| Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania.
mSphere. 2019 Aug 21;4(4). pii: 4/4/e00408-19. doi: 10.1128/mSphere.00408-19.
|
Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania
pSP72
|
| pLPhygSaCas9 | Staphylococcus aureus Cas9 (Other) | |
Matlashewski
| Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania.
mSphere. 2019 Aug 21;4(4). pii: 4/4/e00408-19. doi: 10.1128/mSphere.00408-19.
|
Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania
pSP72
NEWENTRY
|
| PCV-Cas9 | PCV2 (Other), Cas9 (Other) | |
Gordon
| Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template.
Commun Biol. 2018 May 31;1:54. doi: 10.1038/s42003-018-0054-2. eCollection 2018.
|
Expresses Cas9 with an N-terminal HUH-tag called PCV2
pTD68
ORF1 PCV2_gp1
|
| Cas9-PCV | PCV2 (Other), Cas9 (Other) | |
Gordon
| Increasing Cas9-mediated homology-directed repair efficiency through covalent tethering of DNA repair template.
Commun Biol. 2018 May 31;1:54. doi: 10.1038/s42003-018-0054-2. eCollection 2018.
|
Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli
pTD68
ORF1 PCV2_gp1
|
| spCas9-NLS-MAV | cas9 (Other) | T7 |
Akavia
| Double-Stranded Biotinylated Donor Enhances Homology-Directed Repair in Combination with Cas9 Monoavidin in Mammalian Cells.
CRISPR J. 2018 Dec;1:414-430. doi: 10.1089/crispr.2018.0045.
|
SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)
pEC-K-MBP
|
| pAT-9218 | SpCas9 (Other) | TetR/TetA |
Welker
|
NGG
(unpublished)
|
Bacterial SpCas9 expression
p15A
|
| pET-21a_FnCpf1_2xNLS_protein_expression | FnCpf1_2xNLS (Synthetic) | |
Wolfe
| Enhanced Cas12a editing in mammalian cells and zebrafish.
Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184.
|
Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone
pET-21 a
|
| pFD115 | Ptet (Other), cas9 (Other), term PpflB sgRNA (BsaI) (Synthetic) | Ptet |
Bikard
| Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels.
Methods. 2019 Aug 1. pii: S1046-2023(18)30497-3. doi: 10.1016/j.ymeth.2019.07.024.
|
pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
pLZ12
|
| pCold CL7-Cas9 | Cas9 (Other) | |
Liu
| Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins.
Commun Biol. 2019 May 3;2:161. doi: 10.1038/s42003-019-0402-x. eCollection 2019.
|
obtaining full Cas9 RNP
pCold I
|
| pLdCN2 | gRNA and Cas9 (Other) | |
Matlashewski
| Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania.
Methods Mol Biol. 2020;2116:199-224. doi: 10.1007/978-1-0716-0294-2_14.
|
Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania
pLdCN
|
| pLQ-KO-tgt50 | Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50 (Other) | |
Yang
| CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus.
Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):764-770. doi: 10.1093/abbs/gmx074.
|
CRISPR-Cas9 based efficient genome editing in S. aureus
ColE1 origin, AmpR, Rep(+), cat resistance
|
| pET-FLAG-eSpCas9 | eSpCas9 (Other) | T7 |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression of increased fidelity eSpCas9 in bacterial cells
pMJ806-like
|
| pET-FLAG-SpCas9-HF1 | SpCas9-HF1 (Other) | T7 |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression of increased fidelity SpCas9-HF1 in bacterial cells
pMJ806-like
|
| pET-FLAG-B-eSpCas9 | B-eSpCas9 (Other) | T7 |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
pMJ806-like
|
| pET-FLAG-eSpCas9-plus | eSpCas9-plus (Other) | T7 |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
pMJ806-like
|
| pET-FLAG-SpCas9-HF1-plus | SpCas9-HF1-plus (Other) | T7 |
Welker
| Blackjack mutations improve the on-target activities of all increased fidelity variants of SpCas9 without compromising their fidelities
Nature Communications, 1223, 2020
|
Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
pMJ806-like
|
| pIgnaviCas9 | IgnaviCas9 (Other) | T7 promoter |
Quake
| Nucleic acid cleavage with a hyperthermophilic Cas9 from an uncultured Ignavibacterium.
Proc Natl Acad Sci U S A. 2019 Oct 28. pii: 1904273116. doi: 10.1073/pnas.1904273116.
|
Expresses IgnaviCas9 in E. coli
pMJ806
|
| pCAH01Sp::Cas9 | Sp Cas9 (Other) | Ptet |
Henard
| Development of a CRISPR/Cas9 System for Methylococcus capsulatus In Vivo Gene Editing.
Appl Environ Microbiol. 2019 May 16;85(11). pii: AEM.00340-19. doi: 10.1128/AEM.00340-19. Print 2019 Jun 1.
|
Ptet-controlled expression of S. pyogenes Cas9
pCAH01SpR
|
| pCasTet-λ | TetR and pTetR/TetO (Other) | tet promoter |
Johnston
| Systematic evasion of the restriction-modification barrier in bacteria.
Proc Natl Acad Sci U S A. 2019 May 16. pii: 1820256116. doi: 10.1073/pnas.1820256116.
|
Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
Plasmid #62225
|
| pSDMA65 | Hygromycin (HYG) (Synthetic) | ACT1 |
Fraser
|
Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.
(unpublished)
|
Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.
pBlueScript II SK(-)
|
| pCRISPomyces-Sth1Cas9 | Sth1Cas9 (Other) | rpsL(XC)-BbsI |
Wong
| Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes.
Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021.
|
Contains codon-optimized Sth1Cas9 and gRNA for streptomyces
pKC1139
|
| pCRISPomyces-SaCas9 | SaCas9 (Other) | rpsL(XC)-BbsI |
Wong
| Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes.
Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021.
|
Contains codon-optimized SaCas9 and gRNA for streptomyces
pKC1139
|
| pCRISPomyces-FnCpf1 | FnCpf1 (Other) | rpsL(XC)-BbsI |
Wong
| Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes.
Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021.
|
Contains codon-optimized FnCpf1 and gRNA for streptomyces
pKC1139
|
| pET-His6-FnCas9GFP | HA-NLS-FnCas9 (Synthetic) | T7 |
Chakraborty
| Francisella novicida Cas9 interrogates genomic DNA with very high specificity and can be used for mammalian genome editing.
Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 10.1073/pnas.1818461116. Epub 2019 Sep 30.
|
expression of FnCas9-GFP in bacterial cells
pET-His6-GFP-TEV-LIC
|
| pKM197 | Upstream & Downstream pyrE deletion region (Other) | |
Sorg
|
Modified / Updated Clostridium difficile CRISPR-Cas9 genome editing plasmids
(unpublished)
|
pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
pJS116
|
| pSCBE3-HF | HF-BE3 (Other) | |
Sun
| Base editing in Streptomyces with Cas9-deaminase fusions
BioRxiv 630137
|
Expresses HF-BE3 CRISPR base editor and gRNA scaffold in Streptomyces
pYH7
|
| pSEVA421-Cas9tr | tracrRNA-Cas9 (Other) | |
de Lorenzo
| Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery.
ACS Synth Biol. 2019 Sep 20;8(9):2186-2193. doi: 10.1021/acssynbio.9b00293. Epub 2019 Sep 3.
|
Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes
pSEVA421
|
| pCRISPRx-Sth1Cas9-L5 | Sth1Cas9 (Other), TetR, L5 attP, L5 Int, KanR | |
Bitter
| Efficient genome editing in pathogenic mycobacteria using Streptococcus thermophilus CRISPR1-Cas9
Tuberculosis (2020) 101983
|
Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria
PLJR962
|
| pCB578 | Cas9 and tracrRNA (Other), repeat-spacer-repeat array | |
Beisel
| Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods.
Biotechnol J. 2019 Mar;14(3):e1700583. doi: 10.1002/biot.201700583. Epub 2018 Sep 20.
|
E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array
colE1, pAM beta
|
| pCgCas9_recT | Cas9 (Other) | |
Yang
| De Novo Engineering of Corynebacterium glutamicum for l-Proline Production.
ACS Synth Biol. 2020 Jul 17;9(7):1897-1906. doi: 10.1021/acssynbio.0c00249. Epub 2020 Jul 6.
|
Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr
pJYS1Peftu
|
| pJH1 | | |
Poulter
| The application of the CRISPR-Cas9 system in Pseudomonas syringae pv. actinidiae.
J Med Microbiol. 2020 Mar;69(3):478-486. doi: 10.1099/jmm.0.001124. Epub 2020 Jan 14.
|
encodes CRISPR-Cas9 system derived from plasmid pCas9
pCas9
|
| pEM-Cas9HF1-D1135E | Cas9HF1-D1135E | pTet |
Lynch
| CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
|
aTc inducible Cas9HF1 with D1135E mutation
p15A
|
| pEM-eCas9 | eCas9 | pTet |
Lynch
| CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
|
aTc inducible eCas9
p15A
|
| pEM-eCas9-D1135E | eCas9-D1135E | pTet |
Lynch
| CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
|
aTc inducible eCas9 with D1135E mutation
p15A
|
| pEM-OptiCas9 | OptiCas9 | pTet |
Lynch
| CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
|
aTc inducible OptiCas9
p15A
|
| pEM-OptiCas9-D1135E | OptiCas9-D1135E | pTet |
Lynch
| CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
|
aTc inducible OptiCas9 with D1135E mutation
p15A
|
| pEM-HypaCas9 | HypaCas9 | pTet |
Lynch
| CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
|
aTc inducible HypaCas9
p15A
|
| pEM-HypaCas9-D1135E | HypaCas9-D1135E | pTet |
Lynch
| CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
|
aTc inducible HypaCas9 with D1135E mutation
p15A
|
| pEM-Cas9-D1135E | Cas9-D1135E | pTet |
Lynch
| CRISPR-Cas "Non-Target" Sites Inhibit On-Target Cutting Rates.
CRISPR J. 2020 Dec;3(6):550-561. doi: 10.1089/crispr.2020.0065.
|
aTc inducible Cas9 with D1135E mutation
p15A
|
| pDS05 | wtCas9 (Other), gfp | |
Veening
|
Harnessing CRISPR-Cas9 for genome editing in Streptococcus pneumoniae
(unpublished)
|
A S. pneumoniae not integrated, temperature sensitive plasmid that carries the CRISPR system; a Zn inducible wtCas9 and a sgRNA which has its spacer sequence replaced by gfp gene
pcdf1-b
|
| pJV53-Cas12a | FnCpf1 (Other) | TetO |
Sun
| CRISPR-Cas12a-Assisted Recombineering in Bacteria.
Appl Environ Microbiol. 2017 Aug 17;83(17). pii: AEM.00947-17. doi: 10.1128/AEM.00947-17. Print 2017 Sep 1.
|
CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61
pJV53
|
| pMV261-Cas12a | FnCpf1 (Other) | TetO |
Sun
| CRISPR-Cas12a-Assisted Recombineering in Bacteria.
Appl Environ Microbiol. 2017 Aug 17;83(17). pii: AEM.00947-17. doi: 10.1128/AEM.00947-17. Print 2017 Sep 1.
|
expresses codon-optimized FnCpf1
pMV261
|
| pNHEJ-Cas12a | the MmNHEJ machinery (MMAR_4573, MMAR_4574, and MMAR_4575) (Other) | |
Sun
| A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis.
mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19.
|
CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum
pJV53-Cas12a
|
| pNHEJ-Cas12a-recX | recX (Other) | Pmyc |
Sun
| A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis.
mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19.
|
CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses FnCpf1, NHEJ machinery of M. marinum and recX
pNHEJ-Cas12a
|
| pNHEJ-Cas9-recX | cas9 (Other) | TetO |
Sun
| A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis.
mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 10.1128/mBio.02364-19.
|
CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium smegmatis. Helper plasmid expresses Cas9, NHEJ machinery of M. marinum and recX
pNHEJ-Cas12a-recX
|
| pET SUMO NL-FKBP-Cas9 | FKBP F36V (Homo sapiens) | |
Choudhary
| Chemogenetic System Demonstrates That Cas9 Longevity Impacts Genome Editing Outcomes
ACS Cent. Sci. 2020. November 18, 2020
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Bacterial expression, protein expression
pET
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