PurposeA crRNA expression plasmid for targeting a specific sequence.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42875||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2254
- Total vector size (bp) 2433
Vector typeBacterial Expression, CRISPR ; E.coli
Growth in Bacteria
Copy numberHigh Copy
This plasmid is also known as pDB129.
This plasmid contains a duplicated spacer insertion site (4 BsaI sites instead of 2). Please note that these extra sites are eliminated upon treatment with BsaI, and the depositing lab has used this version extensively without issue.
The HME63 strain is available from the from the Court Lab:
For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCRISPR was a gift from Luciano Marraffini (Addgene plasmid # 42875 ; http://n2t.net/addgene:42875 ; RRID:Addgene_42875)
For your References section:RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508. 10.1038/nbt.2508 PubMed 23360965