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RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA
Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2508.
PubMed Article

A two plasmid system for editing bacterial genomes using an RNA-guided Cas9 nuclease.

Marraffini Lab pCas9 Marraffini Lab pCRISPR


Marraffini pCRISPR protocol 125.6 KB

Plasmids from Article

ID Plasmid Purpose
42875pCRISPR A crRNA expression plasmid for targeting a specific sequence.
42876pCas9Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide
44505pCRISPR::rpsLA crRNA expression plasmid specific to the rpsL allele.

Antibodies from Article