Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection
The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled screens. This collection and library can be used for screening transcription factors for applications such as cellular programming or cancer drug resistance.
Protocols & References
A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. doi: 10.1016/j.cell.2022.11.026.
- TF ORF barcode PCR for NGS QC and bulk screening (85 KB)
- TF ORF barcode dial out PCR for 10X scRNA-seq (59.2 KB)
Plasmids
At this time, only a portion of the full collection of individual clones are available. More plasmids will be released over time. Please continue to check the table below, sign up for an Addgene alert, or contact us at [email protected] and let us know what construct you are interested in. Due to the extensive size of this collection, some plasmids will not be fully sequence verified by Addgene before being made available. If an Addgene verified full plasmid sequence is not available, please contact us to request that we sequence it before ordering.
| ID | Plasmid | Gene/Insert |
|---|
Pooled Library
The Multiplexed Overexpression of Regulatory Factors (MORF) pooled library is a comprehensive collection of human transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification during screening.
| Library | ID | PI | Description |
|---|---|---|---|
| 137000, 192821, 1000000218 | A collection of human transcription factor open reading frames with or without GFP and mCherry controls. |
Do you have suggestions for other plasmids that should be added to this list?
Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!