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Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection

The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones, or the pooled library containing the entire pooled collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification of the transcription factor isoform in pooled screens. This collection and library can be used for screening transcription factors for applications such as cellular programming or cancer drug resistance.

A diagram of lentiviral plasmids arrayed in a multi-well plate, labeled “arrayed ORF library”. To the right is an image of lentiviral plasmids inducing cellular differentiation into different cell types.

Protocols & References

A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. doi: 10.1016/j.cell.2022.11.026.


At this time, only a portion of the full collection of individual clones are available. More plasmids will be released over time. Please continue to check the table below, sign up for an Addgene alert, or contact us at [email protected] and let us know what construct you are interested in. Due to the extensive size of this collection, some plasmids will not be fully sequence verified by Addgene before being made available. If an Addgene verified full plasmid sequence is not available, please contact us to request that we sequence it before ordering.

ID Plasmid Gene/Insert

Pooled Library

The Multiplexed Overexpression of Regulatory Factors (MORF) pooled library is a comprehensive collection of human transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification during screening.

Library ID PI Description

MORF library

137000, 192821, 1000000218


A collection of human transcription factor open reading frames with or without GFP and mCherry controls.

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