Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library
(Pooled Library #1000000218, #192821)
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Purpose
The Multiplexed Overexpression of Regulatory Factors (MORF) pooled library is a comprehensive collection of human transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to simplify factor identification during screening. Individual plasmids are also available on the MORF Collection page.
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Vector Backbone
pLX_TRC317 - Constructed by inserting ORF and barcode in pLEX_307
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Pooled Library | 1000000218 | MORF library with GFP and mCherry controls + 2 control plasmids
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1 | $430 | Add to Cart | |
Pooled Library | 192821 | MORF library without GFP and mCherry controls | 1 | $380 | Add to Cart |
Library Details
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SpeciesHuman
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ORFs3,548 isoforms of 1,836 genes
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Insert size range250 - 10,000 bp
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
For the Kit with GFP and mCherry controls ONLY: You will also receive bacterial stabs of TFORF3549 (GFP, Addgene ID #145025) and TFORF3550 (mCherry, Addgene ID #145026) plasmids. Please do NOT order these plasmids in addition to this library.
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Volume∼20 µl
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Pooled library #137000 contains low levels of the TFORF3549 (GFP) and TFORF3550 (mCherry) control plasmids since cells that express the control plasmids will grow faster than cells with differentiated phenotypes in a pooled screen. The individual GFP and mCherry plasmids are also provided as bacterial agar stabs in the kit. If you have difficulty amplifying the control plasmids or if you would like higher amounts of mCherry or GFP, you can choose to add in more control plasmids separately, after library amplification. For example, add 3 ng of each control plasmid (GFP and mCherry) to 10 ug of the amplified pooled library.
For library transformation and amplification, please refer to Joung et al. Nat Protoc. 2017, with 2 modifications:
- 1. As this is an ORF library, increasing the coverage (i.e., colonies/ORF) to 5-10 electroporations is recommended to minimize skewing.
- 2. Plate 1 electroporation onto 2 large LB agar plates to help minimize colony contact.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Multiplexed Overexpression of Regulatory Factors (MORF) Library was a gift from Feng Zhang (Addgene #) -
For your References section:
A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F Cell. 2023 Jan 5; 186(1):209-229. doi: 10.1016/j.cell.2022.11.026.