| pCMV-BE1 | BE1 (Rattus norvegicus) | CMV | |
Liu
| Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.
Nature. 2016 Apr 20. doi: 10.1038/nature17946.
|
Expresses BE1 with C-terminal NLS in mammalian cells
CMV
|
| pCMV-BE2 | BE2 (Rattus norvegicus) | CMV | |
Liu
| Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.
Nature. 2016 Apr 20. doi: 10.1038/nature17946.
|
Expresses BE2 in mammalian cells
CMV
|
| pCMV-BE3 | BE3 (Rattus norvegicus) | CMV | |
Liu
| Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.
Nature. 2016 Apr 20. doi: 10.1038/nature17946.
|
Expresses BE3 in mammalian cells
CMV
|
| pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT) | SpCas9 (Other) | pCMV | Neomycin (select with G418) |
Kondo
| Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.
Science. 2016 Aug 4. pii: aaf8729.
|
Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells
pcDNA3.1
|
| MO91-dCas9-AID(P182X) | dCas9-AID P182X (Synthetic) | MSCV | Puromycin |
Chang
| Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells.
Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10.
|
Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.
M091
|
| pJL-SaBE3 | SaBE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses SaBE3 in mammalian cells
CMV
|
| pJL-SaKKH-BE3 | SaKKH-BE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses SaKKH-BE3 in mammalian cells [Edit]
CMV
|
| pBK-VQR-BE3 | VQR-BE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses VQR-BE3 in mammalian cells
CMV
|
| pBK-EQR-BE3 | EQR-BE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses EQR-BE3 in mammalian cells
CMV
|
| pBK-VRER-BE3 | VRER-BE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses VRER-BE3 in mammalian cells
CMV
|
| pBK-YE1-BE3 | YE1-BE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses YE1-BE3 in mammalian cells
CMV
|
| pBK-EE-BE3 | EE-BE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses EE-BE3 in mammalian cells
CMV
|
| pBK-YE2-BE3 | YEE-BE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses YE2-BE3 in mammalian cells
CMV
|
| pBK-YEE-BE3 | YEE-BE3 (Rattus norvegicus) | CMV | |
Liu
| Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions.
Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.
|
Expresses YEE-BE3 in mammalian cells
CMV
|
| pGH335_MS2-AID*Δ-Hygro | MS2-AID*Δ and Hygromycin resistance | | Hygromycin |
Bassik
| Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
|
lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker
Addgene #61426
|
| pGH183_MS2-AIDΔDead-Hygro | MS2-AIDΔDead and Hygromycin resistance | | Hygromycin |
Bassik
| Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
|
lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker
Addgene #61426
|
| pGH156_MS2-AID-Hygro | MS2-AID and Hygromycin resistance | | Hygromycin |
Bassik
| Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
|
lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker
Addgene #61426
|
| pGH153_MS2-AIDΔ-Hygro | MS2-AIDΔ and Hygromycin resistance | | Hygromycin |
Bassik
| Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.
Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
|
lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker
Addgene #61426
|
| pCMV-HF-BE3 | HF-BE3 (Homo sapiens) | CMV | |
Liu
| Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery.
Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
|
Expresses HF-BE3 with C-terminal NLS in mammalian cells
pCMV
|
| pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS | rAPOBEC1-nCas9 (D10A)-UGI-NLS (Rattus norvegicus) | T7 | |
Kim
| Highly efficient RNA-guided base editing in mouse embryos.
Nat Biotechnol. 2017 Feb 27. doi: 10.1038/nbt.3816.
|
Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.
pET28a
|
| BE4 | BE (Rattus norvegicus) | CMV | |
Liu
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.
|
Expresses BE4 in mammalian cells
pCMV
|
| AID-BE3 | activation induced cytidine deaminase (Homo sapiens) | CMV | |
Liu
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.
|
Expresses AID-BE3 in mammalian cells
pCMV
AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
|
| CDA1-BE3 | CDA1-BE3 (Synthetic) | CMV | |
Liu
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.
|
Expresses CDA1-BE3 in mammalian cells
pCMV
|
| SaBE4 | SaBE4 (Rattus norvegicus) | CMV | |
Liu
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.
|
Expresses SaBE4 in mammalian cells
pCMV
|
| BE4-Gam | BE4-Gam (Rattus norvegicus) | CMV | |
Liu
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.
|
Expresses BE4-Gam in mammalian cells
pCMV
|
| BE3-Gam | BE3-Gam (Rattus norvegicus) | CMV | |
Liu
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.
|
Expresses BE3-Gam in mammalian cells
pCMV
|
| SaBE4-Gam | SaBE4-Gam (Rattus norvegicus) | CMV | |
Liu
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.
|
Expresses SaBE4-Gam in mammalian cells
pCMV
|
| SaBE3-Gam | SaBE3-Gam (Rattus norvegicus) | CMV | |
Liu
| Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.
|
Expresses SaBE3-Gam in mammalian cells
pCMV
|
| pCMV-eBE-S1 | eBE-S1 (Rattus norvegicus) | CMV | |
Chen
| Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor.
Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111.
|
Expresses eBE-S1 in mammalian cells
pCMV
|
| pCMV-eBE-S3 | eBE-S3 (Rattus norvegicus) | CMV | |
Chen
| Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor.
Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111.
|
Expresses eBE-S3 in mammalian cells
pCMV
|
| pCMV-ABE6.3 | ABE6.3 (Synthetic) | CMV | |
Liu
| Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage.
Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.
|
expresses ABE6.3 in mammalian cells
pBR322/CMV
|
| pCMV-ABE7.8 | ABE7.8 (Synthetic) | CMV | |
Liu
| Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage.
Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.
|
expresses ABE7.8 in mammalian cells
pBR322/CMV
|
| pCMV-ABE7.9 | ABE7.9 (Synthetic) | CMV | |
Liu
| Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage.
Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.
|
expresses ABE7.9 in mammalian cells
pBR322/CMV
|
| pCMV-ABE7.10 | ABE7.10 (Synthetic) | CMV | |
Liu
| Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage.
Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.
|
expresses ABE7.10 in mammalian cells
pBR322/CMV
|
| pCMV-dCpf1-BE0 | dLbCpf1-BE0 (Rattus norvegicus) | CMV | |
Chen
| Base editing with a Cpf1-cytidine deaminase fusion.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
|
Expresses dLbCpf1-BE0 in mammalian cells
CMV
|
| pCMV-dLbCpf1-BE0ΔiNLS | dLbCpf1-BE0ΔiNLS (Rattus norvegicus) | CMV | |
Chen
| Base editing with a Cpf1-cytidine deaminase fusion.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
|
Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells
CMV
|
| pCMV-dCpf1-BE | dLbCpf1-BE (Rattus norvegicus) | CMV | |
Chen
| Base editing with a Cpf1-cytidine deaminase fusion.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
|
Expresses dLbCpf1-BE in mammalian cells
CMV
|
| pCMV-dCpf1-BE-YE | dLbCpf1-BE-YE (Rattus norvegicus) | CMV | |
Chen
| Base editing with a Cpf1-cytidine deaminase fusion.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
|
Expresses dLbCpf1-BE-YE in mammalian cells
CMV
|
| pCMV-dCpf1-BE-YEE | dLbCpf1-BE-YEE (Rattus norvegicus) | CMV | |
Chen
| Base editing with a Cpf1-cytidine deaminase fusion.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
|
Expresses dLbCpf1-BE-YEE in mammalian cells
CMV
|
| pCMV-dCpf1-eBE | dLbCpf1-eBE (Rattus norvegicus) | CMV | |
Chen
| Base editing with a Cpf1-cytidine deaminase fusion.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
|
Expresses dLbCpf1-eBE in mammalian cells
CMV
|
| pCMV-dCpf1-eBE-YE | dLbCpf1-eBE-YE (Rattus norvegicus) | CMV | |
Chen
| Base editing with a Cpf1-cytidine deaminase fusion.
Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
|
Expresses dLbCpf1-eBE-YE in mammalian cells
CMV
|
| pCMV-ABE | ABE7.10 (Synthetic) | CMV | |
Huang
| Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing.
Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7.
|
For expression of ABE7.10 in mammalian cells
PBR322
|
| pWT063a | TRESG-BE3 (Synthetic) | | |
Liu
| Rewritable multi-event analog recording in bacterial and mammalian cells.
Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992.
|
W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells
pACK129
|
| pWT065a | (TCF-LEF)7-BE3 (Synthetic) | | |
Liu
| Rewritable multi-event analog recording in bacterial and mammalian cells.
Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992.
|
W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells
pACK129
|
| pWT065c | (TCF-LEF)7-BE3-P2A-Luc (Synthetic) | | |
Liu
| Rewritable multi-event analog recording in bacterial and mammalian cells.
Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992.
|
W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells
pACK129
|
| xCas9(3.7)-BE3 | xCas9(3.7)-BE3 (Synthetic) | | |
Liu
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
|
Mammalian expression vector for xCas9(3.7)-BE3
pCMV
Tag
/ Fusion Protein- NLS (N terminal on insert)
|
| xCas9(3.7)-BE4 | xCas9(3.7)-BE4 (Synthetic) | | |
Liu
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
|
Mammalian expression vector for xCas9(3.7)-BE4
pCMV
Tag
/ Fusion Protein- NLS (N terminal on insert)
|
| xCas9(3.7)-ABE(7.10) | xCas9(3.7)-ABE(7.10) (Synthetic) | | |
Liu
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
|
Mammalian expression vector for xCas9(3.7)-ABE
pCMV
Tag
/ Fusion Protein- NLS (N terminal on insert)
|
| xCas9(3.6)-BE3 | xCas9(3.6)-BE3 (Synthetic) | | |
Liu
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
|
Mammalian expression vector for xCas9(3.6)-BE3
pCMV
Tag
/ Fusion Protein- NLS (N terminal on insert)
|
| xCas9(3.6)-BE4 | xCas9(3.6)-BE4 (Synthetic) | | |
Liu
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
|
Mammalian expression vector for xCas9(3.6)-BE4
pCMV
Tag
/ Fusion Protein- NLS (N terminal on insert)
|
| xCas9(3.6)-ABE(7.10) | xCas9(3.6)-ABE(7.10) (Synthetic) | | |
Liu
| Evolved Cas9 variants with broad PAM compatibility and high DNA specificity.
Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.
|
Mammalian expression vector for xCas9(3.6)-ABE
pCMV
Tag
/ Fusion Protein- NLS (N terminal on insert)
|
| A3Ai-Cas9n-UGI-NLS | Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (Homo sapiens) | CMV | |
Harris
| A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.
|
Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
BE3
APOBEC3A
|
| A3Bi-ctd-Cas9n-UGI-NLS | Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (Homo sapiens) | CMV | |
Harris
| A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.
|
Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
BE3
APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
|
| A3Bi-ctd E255A -Cas9n-UGI-NLS | Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (Homo sapiens) | CMV | |
Harris
| A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.
|
Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
BE3
APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
|
| A3Ai E72A-Cas9n-UGI-NLS | Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (Homo sapiens) | CMV | |
Harris
| A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells.
Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.
|
Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
BE3
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pCMV-BE3-2X | BE3-2X (Synthetic) | CMV | |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
CMV expression vector for BE3-2X construct (not codon optimized)
pCMV
|
| pCMV-BE3-FNLS | BE3-FNLS (Synthetic) | CMV | |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
CMV expression vector for BE3-FNLS construct (not codon optimized)
pCMV
Tag
/ Fusion Protein- 3X FLAG (N terminal on insert)
|
| pLenti-BE3-P2A-Puro | BE3 (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of BE3 (not codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-BE3RA-P2A-Puro | BE3RA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-2X-P2A-Puro | BE3RA-2X (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-FNLS-P2A-Puro | BE3RA-FNLS (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (N terminal on insert)
|
| pLenti-BE3-PGK-Puro | BE3 (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of BE3 (not codon optimized)
pLL3.3
|
| pLenti-BE3RA-PGK-Puro | BE3RA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)
pLL3.3
|
| pLenti-FNLS-PGK-Puro | BE3RA-FNLS (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)
pLL3.3
Tag
/ Fusion Protein- 3X FLAG (N terminal on insert)
|
| pLenti-TRE3G-BE3-PGK-Puro | BE3 (Synthetic) | TRE3G | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)
pLL3.3
|
| pLenti-TRE3G-BE3RA-PGK-Puro | BE3RA (Synthetic) | TRE3G | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)
pLL3.3
|
| pLenti-TRE3G-FNLS-PGK-Puro | BE3RA-FNLS (Synthetic) | TRE3G | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)
pLL3.3
Tag
/ Fusion Protein- 3X FLAG (N terminal on insert)
|
| pLenti-BE3-P2A-GFP-PGK-Puro | BE3 (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-BE3RA-P2A-GFP-PGK-Puro | BE3RA (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-FNLS-P2A-GFP-PGK-Puro | BE3RA-FNLS (Synthetic) | EF1s | Puromycin ; GFP |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (N terminal on insert)
|
| pLenti-xBE3-P2A-Puro | xCas9(3.7)-BE3 (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells
Adapted from lentiCRISPRv1
|
| pLenti-xFNLS-P2A-Puro | xFNLS(3.7) (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (N terminal on insert)
|
| pLenti-xF2X-P2A-Puro | xFNLS-2X(3.7) (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
Tag
/ Fusion Protein- 3X FLAG (N terminal on insert)
|
| pCMV_BE4max | BE4max (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
C:G-to-T:A base editing
pCMV
|
| pCMV_AncBE4max | AncBE4max (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
C:G-to-T:A base editing
pCMV
|
| pCMV_ABEmax | ABEmax (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
A:T-to-G:C base editing
pCMV
|
| pCMV_BE4max_3xHA | BE4max_3xHA (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
C:G-to-T:A base editing
pCMV
Tag
/ Fusion Protein- 3xHA (C terminal on insert)
|
| pCMV_AncBE4max_3xHA | AncBE4max_3xHA (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
C:G-to-T:A base editing
pCMV
Tag
/ Fusion Protein- 3xHA (C terminal on insert)
|
| pCMV_ABEmax_3xHA | ABEmax_3xHA (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
A:T-to-G:C base editing
pCMV
Tag
/ Fusion Protein- 3xHA (C terminal on insert)
|
| pCMV_BE4max_P2A_GFP | BE4max_P2A_GFP (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
C:G-to-T:A base editing
pCMV
|
| pCMV_AncBE4max_P2A_GFP | AncBE4max_P2A_GFP (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
C:G-to-T:A base editing
pCMV
|
| pCMV_ABEmax_P2A_GFP | ABEmax_P2A_GFP (Homo sapiens) | CMV | |
Liu
| Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
|
A:T-to-G:C base editing
pCMV
|
| pLL1 sgRNA(MS2)-MS2-AIDmono | AID mono mutant (Homo sapiens) | hU6,CBh | |
Meng
| Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
|
empty sgRNA cloning vector with MS2-AIDmono
pX330
AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
|
| pLL2 sgRNA(MS2)-MS2-AID3c | AID mono 3c mutant (Homo sapiens) | hU6,CBh | |
Meng
| Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
|
empty sgRNA cloning vector with MS2-AID3c
pX330
AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
|
| pLL3 sgRNA(MS2)-MS2-AID3f | AIDmono 3f mutant (Homo sapiens) | hU6,CBh | |
Meng
| Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
|
empty sgRNA cloning vector with MS2-AID3f
pX330
AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
|
| pLL4 sgRNA(MS2)-MS2-hA3a | human Apobec3a (Homo sapiens) | hU6,CBh | |
Meng
| Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
|
empty sgRNA cloning vector with MS2-humanA3a
pX330
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pLL5 sgRNA(MS2)-MS2-hA3a D131A | human Apobec3a D131A mutant (Homo sapiens) | hU6,CBh | |
Meng
| Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
|
empty sgRNA cloning vector with MS2-humanA3a D131A
pX330
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pLL6 sgRNA(MS2)-MS2-hA3a D131E | human Apobec3a D131E mutant (Homo sapiens) | hU6,CBh | |
Meng
| Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation.
Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.
|
empty sgRNA cloning vector with MS2-humanA3a D131E
pX330
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pCMV-BE3-FNLS(RA) | BE3-FNLS (Synthetic) | CMV | |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
CMV expression vector for BE3-FNLS construct (codon optimized)
pCMV
|
| pLenti-BE4RA-P2A-Puro | BE4RA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-BE4GamRA-P2A-Puro | BE4GamRA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-ABE7.10-P2A-Puro | ABE7.10 (Synthetic) | EF1s | Puromycin |
Dow
|
Dow Lab Unpublished Base Editors
(unpublished)
|
Lentivirus for constitutive expression of ABE7.10 (not codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-ABERA-P2A-Puro | ABERA (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)
Adapted from lentiCRISPRv1
|
| pLenti-xBE4Gam-P2A-Puro | xCas9(3.7)-BE4Gam (Synthetic) | EF1s | Puromycin |
Dow
| Optimized base editors enable efficient editing in cells, organoids and mice.
Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.
|
Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)
Adapted from lentiCRISPRv1
|
| pAAV-ABE-NT-sgRNA | ABE-NT-sgRNA (Other) | | |
Kim
| Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy.
Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27.
|
AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA
pAAV
|
| rat APOBEC1-EGFP | apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Rattus norvegicus) | CMV promoter | Neomycin (select with G418) |
Conticello
| Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells.
RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033.
|
plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein
pEGFP-N1
Apobec1 REPR, apobec-1
|
| pEGFP mCherry-ApoB-EGFP | mCherry-apoB-EGFP (Synthetic) | CMV promoter | Neomycin (select with G418) |
Conticello
| Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells.
RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033.
|
Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP
pEGFP-N1
|
| pAAV-ABE-CT | ABE-CT | | |
Kim
| Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy.
Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27.
|
AAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9
pAAV
|
| pST1374-scFv-APOBEC-UGI-GB1 | scFv-rAPOBEC1-UGI-GB1 (Rattus norvegicus) | CMV | |
Huang
| BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity.
Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4.
|
Expresses scFv-rAPOBEC1-UGI in mammalian cells
pST1374
|
| p3s-ABE7.10 | ABE 7.10 (Other) | CMV | |
Kim
| Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy.
Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27.
|
expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized
pCDNA3.1
|
| pCMV-hA3A-BE3 | hA3A-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3A-BE3 in mammalian cells
CMV
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pCMV-hA3B-BE3 | hA3B-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3B-BE3 in mammalian cells
CMV
APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
|
| pCMV-hA3C-BE3 | hA3C-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3C-BE3 in mammalian cells
CMV
APOBEC3C A3C, APOBEC1L, ARDC2, ARDC4, ARP5, PBI, bK150C2.3
|
| pCMV-hA3D-BE3 | hA3D-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3D-BE3 in mammalian cells
CMV
APOBEC3D A3D, APOBEC3DE, APOBEC3E, ARP6
|
| pCMV-hA3F-BE3 | hA3F-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3F-BE3 in mammalian cells
CMV
APOBEC3F A3F, ARP8, BK150C2.4.MRNA, KA6
|
| pCMV-hA3G-BE3 | hA3G-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3G-BE3 in mammalian cells
CMV
APOBEC3G A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1
|
| pCMV-hA3H-BE3 | hA3H-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3H-BE3 in mammalian cells
CMV
APOBEC3H A3H, ARP-10, ARP10
|
| pCMV-hAID-BE3 | hAID-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hAID-BE3 in mammalian cells
CMV
AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
|
| pCMV-hA1-BE3 | hA1-BE3 (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA1-BE3 in mammalian cells
CMV
APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
|
| pCMV-mA3-BE3 | mA3-BE3 (Mus musculus) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses mA3-BE3 in mammalian cells
CMV
Apobec3 Apobec, Arp3, BC003314, Cem15, Gm20117, Rf, Rfv, Rfv3
|
| pCMV-mAID-BE3 | mAID-BE3 (Mus musculus) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses mAID-BE3 in mammalian cells
CMV
Aicda A, Aid, Arp2
|
| pCMV-mA1-BE3 | mA1-BE3 (Mus musculus) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses mA1-BE3 in mammalian cells
CMV
Apobec1 Cdar1
|
| pCMV-cAICDA-BE3 | cAICDA-BE3 (Other) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses cAICDA-BE3 in mammalian cells
CMV
aicda aid
|
| pCMV-hA3A-BE3-D131Y | hA3A-BE3-D131Y (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3A-BE3-D131Y in mammalian cells
CMV
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pCMV-hA3A-BE3-Y132D | hA3A-BE3-Y132D (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3A-BE3-Y132D in mammalian cells
CMV
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pCMV-hA3A-BE3-C101S | hA3A-BE3-C101S (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3A-BE3-C101S in mammalian cells
CMV
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pCMV-hA3A-BE3-C106S | hA3A-BE3-C106S (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3A-BE3-C106S in mammalian cells
CMV
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pCMV-hA3A-BE3-Y130F | hA3A-BE3-Y130F (Homo sapiens) | CMV | |
Chen
| Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion.
Nat Biotechnol., 2018
|
Expresses hA3A-BE3-Y130F in mammalian cells
CMV
APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
|
| pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351) | human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1) (Synthetic) | | |
Joung
| Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
|
CAG promoter expression plasmid for human codon optimized AsBE1.1
pCAG-CFP
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008) | human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4) (Synthetic) | | |
Joung
| Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
|
CAG promoter expression plasmid for human codon optimized AsBE1.4
pCAG-CFP
Tags
/ Fusion Proteins- 2x SV40 NLS (N terminal on insert)
- SV40 NLS (C terminal on insert)
|
| pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352) | DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1) (Synthetic) | | |
Joung
| Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
|
CAG promoter expression plasmid for human codon optimized enAsBE1.1
pCAG-CFP
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2) | DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2) (Synthetic) | | |
Joung
| Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
|
CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
pCAG-CFP
Tags
/ Fusion Proteins- 2x SV40 NLS (N terminal on insert)
- SV40 NLS (C terminal on insert)
|
| pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296) | DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3) (Synthetic) | | |
Joung
| Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
|
CAG promoter expression plasmid for human codon optimized enAsBE1.3
pCAG-CFP
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028) | DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4) (Synthetic) | | |
Joung
| Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
|
CAG promoter expression plasmid for human codon optimized enAsBE1.4
pCAG-CFP
Tags
/ Fusion Proteins- 2x SV40 NLS (N terminal on insert)
- SV40 NLS (C terminal on insert)
|
| pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295) | human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1) (Synthetic) | | |
Joung
| Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
|
CAG promoter expression plasmid for human codon optimized LbBE1.1
pCAG-CFP
Tag
/ Fusion Protein- SV40 NLS (C terminal on insert)
|
| pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293) | human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4) (Synthetic) | CAG | |
Joung
| Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.
Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.
|
CAG promoter expression plasmid for human codon optimized LbBE1.4
pCAG-CFP
Tags
/ Fusion Proteins- 2x SV40 NLS (N terminal on insert)
- SV40 NLS (C terminal on insert)
|
| PB-BE3-NLS | BE3 (Other) | | |
Li
| CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development.
Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4.
|
Stably express BE3 in mammalian cells
PiggyBac-mRFP
|
| PB-BE3-2NLS | BE3 (Other) | | |
Li
| CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development.
Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4.
|
Stably express BE3 in mammalian cells
PiggyBac-mRFP
|
| PB-BE3-3NLS | BE3 (Other) | | |
Li
| CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development.
Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4.
|
Stably express BE3 in mammalian cells
PiggyBac-mRFP
|
| A3Bi-Cas9n-UGI | APOBEC3B L1 intron-Cas9 nickase-UGI (Homo sapiens) | CMV | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction
BE3
|
| A3C-Cas9n-UGI | APOBEC3C-Cas9 nickase-UGI (Homo sapiens) | CMV | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
Base editor made from APOBEC3C
BE3
|
| A3D-Cas9n-UGI | APOBEC3D-Cas9 nickase-UGI (Homo sapiens) | CMV | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
Base editor made from APOBEC3D
BE3
|
| A3F-Cas9n-UGI | APOBEC3F-Cas9 nickase-UGI (Homo sapiens) | CMV | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
Base editor made from APOBEC3F
BE3
|
| A3G-Cas9n-UGI | APOBEC3G-Cas9 nickase-UGI (Homo sapiens) | CMV | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
Base editor made from APOBEC3G
BE3
|
| A3H HapI-Cas9n-UGI | APOBEC3H Haplotype I-Cas9 nickase-UGI (Homo sapiens) | CMV | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
Base editor made from APOBEC3H Haplotype I
BE3
|
| A3H HapII-Cas9n-UGI | APOBEC3H Haplotype II-Cas9 nickase-UGI (Homo sapiens) | CMV | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
Base editor made from APOBEC3H Haplotype II
BE3
|
| A3H HapII R175/6E-Cas9n-UGI | APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI (Homo sapiens) | CMV | |
Harris
| A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes.
Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
|
Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE
BE3
|
| CP1012-CBEmax | rApobec1-nSpCas9 (CP1012) -UGI-UGI (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
C-to-T base editor
pCMV with a BR322 origin
|
| CP1028-CBEmax | rApobec1-nSpCas9 (CP1028) -UGI-UGI (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
C-to-T base editor
pCMV with a BR322 origin
|
| CP1041-CBEmax | rApobec1-nSpCas9 (CP1041) -UGI-UGI (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
C-to-T base editor
pCMV with a BR322 origin
|
| CP1249-CBEmax | rApobec1-nSpCas9 (CP1249) -UGI-UGI (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
C-to-T base editor
pCMV with a BR322 origin
|
| CP1300-CBEmax | rApobec1-nSpCas9 (CP1300) -UGI-UGI (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
C-to-T base editor
pCMV with a BR322 origin
|
| CP1012-ABEmax | ecTadA-ecTadA*(7.10)-nSpCas9 (CP1012) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| CP1028-ABEmax | ecTadA-ecTadA*(7.10)-nSpCas9 (CP1028) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| CP1041-ABEmax | ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| CP1249-ABEmax | ecTadA-ecTadA*(7.10)-nSpCas9 (CP1249) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| CP1300-ABEmax | ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| VRQR-ABEmax | ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| VRER-ABEmax | ecTadA-ecTadA*(7.10)-nSpCas9 (VRER) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| xABEmax | ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| SaABEmax | ecTadA-ecTadA*(7.10)-nSaCas9 (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| SaKKH-ABEmax | ecTadA-ecTadA*(7.10)-nSaCas9 (KKH) (Other) | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV with a BR322 origin
|
| pSI-Target-AID-NG | nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) | | |
Nureki
| Engineered CRISPR-Cas9 nuclease with expanded targeting space.
Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129.
|
Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.
pcDNA3.1
|
| pLV302 | N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE) (Rattus norvegicus) | CMV | |
Schwank
| Treatment of a metabolic liver disease by in vivo genome base editing in adult mice.
Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8.
|
Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells
px601
|
| pLV312.3 | C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3 (Rattus norvegicus) | CMV | |
Schwank
| Treatment of a metabolic liver disease by in vivo genome base editing in adult mice.
Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8.
|
Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells
px601
|
| pcDNA3.1(-)-HF2-BE2 | HF2-BE2 (Synthetic) | CMV | Neomycin (select with G418) |
Huang
| Effective gene editing by high-fidelity base editor 2 in mouse zygotes.
Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5.
|
Expresses HF2-BE2 in mammalian cells
pcDNA3.1(-)
|
| pcDNA3.1(-)-HF2-BE3 | HF2-BE3 (Synthetic) | CMV | Neomycin (select with G418) |
Huang
| Effective gene editing by high-fidelity base editor 2 in mouse zygotes.
Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5.
|
Expresses HF2-BE3 in mammalian cells
pcDNA3.1(-)
|
| pcDNA3.1(-)-ABE7.10 | ABE7.10 (Synthetic) | CMV | Neomycin (select with G418) |
Huang
| Genome-wide profiling of adenine base editor specificity by EndoV-seq.
Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.
|
Expresses ABE7.10 in mammalian cells
pcDNA3.1(-)
|
| CAG-FNLS-T2A-EGFP-ires-puro | FNLS-T2A-EGFP-ires-puro (Other) | | Puromycin ; EGFP |
Otonkoski
|
Otonkoski plasmids 2018
(unpublished)
|
CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
PyCAG
|
| CAG-ABEmax-P2A-EGFP-ires-puro | ABEmax-P2A-EGFP-ires-puro (Other) | | Puromycin ; EGFP |
Otonkoski
|
Otonkoski plasmids 2018
(unpublished)
|
CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
PyCAG
|
| CAG-xFNLS-T2A-EGFP-ires-puro | xFNLS-T2A-EGFP-ires-puro (Other) | | Puromycin ; EGFP |
Otonkoski
|
Otonkoski plasmids 2018
(unpublished)
|
CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
PyCAG
|
| pBT44c | intein-dCas9-ugi fusion (Other) | | |
Liu
| Continuous evolution of base editors with expanded target compatibility and improved activity.
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.
|
BE PACE selection, express C-intein-Cas9-ugi fusion
pBT38
|
| pBT276 | CDA1-BE4max (Other) | | |
Liu
| Continuous evolution of base editors with expanded target compatibility and improved activity.
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.
|
express CDA1-BE4max in mammalian cells
pBT195
|
| pBT277 | evoCDA1-BE4max (Other) | | |
Liu
| Continuous evolution of base editors with expanded target compatibility and improved activity.
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.
|
express evoCDA1-BE4max in mammalian cells
pBT195
|
| pBT281 | evoAPOBEC1-BE4max (Other) | | |
Liu
| Continuous evolution of base editors with expanded target compatibility and improved activity.
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.
|
express evoAPOBEC1-BE4max in mammalian cells
pBT195
|
| nCas9-UGI-NLS-P2A-EGFP (pJUL1001) | nCas9-UGI-NLS-P2A-EGFP (Synthetic) | CAG | |
Joung
| Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors.
Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.
|
CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).
CAG
|
| BE3-P2A-EGFP (pJUL977) | BE3-P2A-EGFP (Rattus norvegicus) | CAG | |
Joung
| Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors.
Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.
|
CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.
CAG
|
| BE3(E63Q)-P2A-EGFP (pRZ189) | BE3(E63Q)-P2A-EGFP (Rattus norvegicus) | CAG | |
Joung
| Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors.
Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.
|
CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).
CAG
|
| SECURE BE3(R33A)-P2A-EGFP (pJUL1085) | BE3(R33A)-P2A-EGFP (Rattus norvegicus) | CAG | |
Joung
| Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors.
Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.
|
CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).
CAG
|
| SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410) | BE3(R33A/K34A)-P2A-EGFP (Rattus norvegicus) | CAG | |
Joung
| Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors.
Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.
|
CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).
CAG
|
| NG-ABEmax | TadA-TadA(evo)-Cas9-NG | | |
Liu
| Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors.
Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.
|
A-to-G base editor
pCMV
|
| dABE | dABE7.10 (Other) | | |
Church
| Enabling large-scale genome editing at repetitive elements by reducing DNA nicking.
Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239.
|
Expresses dead Cas9 (dCas9)-ABE7.10
pCMV_ABE7.10
|
| dCBE4-gam | dCBE4-gam (Other) | | |
Church
| Enabling large-scale genome editing at repetitive elements by reducing DNA nicking.
Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239.
|
Expresses dead Cas9 (dCas9)-CBE4-gam
pCMV
|
| pBT371 | CDA1-BE4max-NG (Other) | | |
Liu
| Continuous evolution of base editors with expanded target compatibility and improved activity.
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.
|
Expresses CDA1-BE4max-NG in mammalian cells
pBT195
|
| pBT372 | evoCDA1-BE4max-NG (Other) | | |
Liu
| Continuous evolution of base editors with expanded target compatibility and improved activity.
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.
|
Expresses evoCDA1-BE4max-NG in mammalian cells
pBT195
|
| pBT375 | evoAPOBEC1-BE4max-NG (Other) | | |
Liu
| Continuous evolution of base editors with expanded target compatibility and improved activity.
Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.
|
Expresses evoAPOBEC1-BE4max-NG in mammalian cells
pBT195
|
| pCMV-ABEmaxAW | ABEmax(TadA E59A, TadA* V106W) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
Expresses ABEmaxAW in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A) | ABEmax(TadA E59A) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
Expresses ABEmax(TadA E59A) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*E59A) | ABEmax(TadA E59A TadA* E59A) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*V106M) | ABEmax(TadA E59A TadA* V106M) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*V106M) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*V106Q) | ABEmax(TadA E59A TadA* V106Q) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*V106Q) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*V106F) | ABEmax(TadA E59A TadA* V106F) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*V106F) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*D108W) | ABEmax(TadA E59A TadA* D108W) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*D108W) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*D108F) | ABEmax(TadA E59A TadA* D108F) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*D108F) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*D108Q) | ABEmax(TadA E59A TadA* D108Q) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*D108M) | ABEmax(TadA E59A TadA* D108M) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*D108M) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*R47Q) | ABEmax(TadA E59A TadA* R47Q) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*R47Q) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*R47F) | ABEmax(TadA E59A TadA* D108F) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*R47F) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*R47W) | ABEmax(TadA E59A TadA* R47W) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*R47W) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA E59A, TadA*R47M) | ABEmax(TadA E59A TadA* R47M) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA E59A, TadA*R47M) in mammalian cells
CMV
|
| pCMV-ABEmax(TadA, TadA*E59A) | ABEmax(TadA E59A TadA* E59A) (Homo sapiens) | | |
Liu
| Analysis and minimization of cellular RNA editing by DNA adenine base editors.
Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.
|
pCMV-ABEmax(TadA, TadA*E59A) in mammalian cells
CMV
|
| pRS0045 | TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI (Synthetic) | CMV promoter | |
Yachie
| Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.
|
Mammalian Target-ACE expression vector
pcDNA3.1
|
| pRS0035 | nCas9-PmCDA1-UGI (Synthetic) | CMV promoter | |
Yachie
| Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.
|
Mammalian Target-AID expression vector
pcDNA3.1
|
| nCas9-UGI control for Target-AID (pRZ1047) | NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Other) | CAG | |
Joung
| CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
|
CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).
CAG
|
| Target-AID (pRZ762) | NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP (Other) | CAG | |
Joung
| CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
|
CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
CAG
|
| miniABEmax (pRZ900) | bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other) | CMV | |
Joung
| CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
|
CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).
CMV
|
| SECURE miniABEmax-K20A/R21A (pJUL1774) | bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other) | CMV | |
Joung
| CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
|
CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
CMV
|
| SECURE miniABEmax-V82G (pJUL1828) | bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other) | CMV | |
Joung
| CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
|
CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).
CMV
|
| hA3A-BE3 (pRZ215) | hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens) | CAG | |
Joung
| CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
|
CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.
CAG
|
| eA3A-BE3 (pRZ206) | hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens) | CAG | |
Joung
| CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
|
CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.
CAG
|
| hAID-BE3 (pRZ352) | hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens) | CAG | |
Joung
| CRISPR DNA base editors with reduced RNA off-target and self-editing activities.
Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.
|
CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.
CAG
|
| BE3-YE1 | BE3-(W90Y-R126E) (Synthetic) | | |
Yang
| Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis.
Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10.
|
Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
X330-U6-Chimeric_BB-CBh-hSpCas9
|
| BE3-hA3A-R128A | BE3-hA3A(R128A) (Synthetic) | | |
Yang
| Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis.
Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10.
|
Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.
X330-U6-Chimeric_BB-CBh-hSpCas9
|
| BE3-hA3A-Y130F | BE3-hA3A(Y130F) (Synthetic) | | |
Yang
| Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis.
Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10.
|
Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
X330-U6-Chimeric_BB-CBh-hSpCas9
|
| ABE7.10-F148A | ABE7.10(F148A) (Synthetic) | | |
Yang
| Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis.
Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10.
|
Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
X330-U6-Chimeric_BB-CBh-hSpCas9
|
| N-4-BE | LjCDA1L1_4 (Other) | U6 with EF-1alpha intron | |
Cheng
| Expanding C-T base editing toolkit with diversified cytidine deaminases.
Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
|
C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9
Px330
|
| N-8-BE | LpCDA1L1_1 (Other) | U6 with EF-1alpha intron | |
Cheng
| Expanding C-T base editing toolkit with diversified cytidine deaminases.
Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
|
C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9
Px330
|
| C-1-BE | PmCDA1L1_4 (Other) | U6 with EF-1alpha intron | |
Cheng
| Expanding C-T base editing toolkit with diversified cytidine deaminases.
Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
|
C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9
Px330
|
| C-7-BE | LpCDA1L1_1 (Other) | U6 with EF-1alpha intron | |
Cheng
| Expanding C-T base editing toolkit with diversified cytidine deaminases.
Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
|
C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9
Px330
|
| C-11-BE | LpCDA1L2_2 (Other) | U6 with EF-1alpha intron | |
Cheng
| Expanding C-T base editing toolkit with diversified cytidine deaminases.
Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
|
C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9
Px330
|
| C-12-BE | LjCDA1L2_1 (Other) | U6 with EF-1alpha intron | |
Cheng
| Expanding C-T base editing toolkit with diversified cytidine deaminases.
Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
|
C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9
Px330
|
| C-13-BE | LpCDA1L1_4 (Other) | U6 with EF-1alpha intron | |
Cheng
| Expanding C-T base editing toolkit with diversified cytidine deaminases.
Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
|
C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9
Px330
|
| AIDx-ID-1058 | nSpyCas9-hAIDx-ID-1058-UGI (Homo sapiens) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus
pcDNA3.1(+)
|
| BE3-NLS-NLS-P2A-BLAST | BE3-2XNLS (Rattus norvegicus) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
pcDNA3.1(+)
|
| S2 1058 AIDx no UGI | nSpyCas9-hAIDx-ID-1058 (Homo sapiens) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.
pcDNA3.1(+)
|
| S2 1058 APOBEC3 no UGI | nSpyCas9-rAPOBEC3-ID-1058 (Rattus norvegicus) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.
pcDNA3.1(+)
|
| S1 1029 CP APOBEC3 no UGI | rAPOBEC3-nSpyCas9 aa 1029 (Rattus norvegicus) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus
pcDNA3.1(+)
|
| S1 1029 AIDx with UGI | hAIDx-nSpyCas9 aa-UGI (Rattus norvegicus) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus
pcDNA3.1(+)
|
| pCMV-ABEMAX-NLS-P2A_BLAST | ABEMax-2XNLS (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance
pcDNA3.1(+)
|
| S2 1058 ABEMax | nSpyCas9-ABEMax aa 1058 (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9
pcDNA3.1(+)
|
| S1 1029 CP ABEMax strategy 1 | ABEMax-nSpyCas9 aa 1029 (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus
pcDNA3.1(+)
|
| S1 1029 CP ABEMax strategy 2 | WT Tada-nSpyCas9 aa 1029-evolved Tada (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)
pcDNA3.1(+)
|
| S1 1029 CP ABEMax strategy 3 | 1029 CP nSpyCas9 ABEMax (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus
pcDNA3.1(+)
|
| S1 1029 miniABEMax V82G strategy1 | mini ABEMax 1029 nSpyCas9 (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus
pcDNA3.1(+)
|
| S2 1058 V82G ABEMax | 1058 nSpyCas9 V82G ABEMax (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9
pcDNA3.1(+)
|
| miniABEMax V82G wt | miniABEMax nSpyCas9 (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSpyCas9 with N-terminal miniABEMax (V82G)
pcDNA3.1(+)
|
| S1 1029 V82G ABEMax Strategy 3 | 1029 V82G ABEMax (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus
pcDNA3.1(+)
|
| ABEMax nSaCas9 | ABEMax nSaCas9 (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus
pcDNA3.1(+)
|
| miniABEMax V82G nSaCas9 | miniABEMax V82G nSaCas9 (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution
pcDNA3.1(+)
|
| nSaCas9 D127 miniABEMax V82G | nSaCas9 D127 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D127 )
pcDNA3.1(+)
|
| nSaCas9 D126 miniABEMax V82G | nSaCas9 D126 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D126 )
pcDNA3.1(+)
|
| nSaCas9 E739 miniABEMax V82G | nSaCas9 E739 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E739 )
pcDNA3.1(+)
|
| nSaCas9 E743 miniABEMax V82G | nSaCas9 E743 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E743 )
pcDNA3.1(+)
|
| nSaCas9 E745 miniABEMax V82G | nSaCas9 E745 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E745 )
pcDNA3.1(+)
|
| nSaCas9 F733 miniABEMax V82G | nSaCas9 F733 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (F733 )
pcDNA3.1(+)
|
| nSaCas9 N730 miniABEMax V82G | nSaCas9 N730 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N730 )
pcDNA3.1(+)
|
| nSaCas9 E125 miniABEMax V82G | nSaCas9 E125 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E125 )
pcDNA3.1(+)
|
| nSaCas9 E131 miniABEMax V82G | nSaCas9 E131 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E131 )
pcDNA3.1(+)
|
| nSaCas9 L132 miniABEMax V82G | nSaCas9 L132 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (L132 )
pcDNA3.1(+)
|
| nSaCas9 N130 miniABEMax V82G | nSaCas9-ABEMax(V82G) N130 (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N130 )
pcDNA3.1(+)
|
| nSaCas9 E735 miniABEMax V82G | nSaCas9 E735 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E735 )
pcDNA3.1(+)
|
| nSaCas9 I744 miniABEMax V82G | nSaCas9 I744 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )
pcDNA3.1(+)
|
| nSaCas9 G129 miniABEMax V82G | nSaCas9 G129 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (G129 )
pcDNA3.1(+)
|
| nSaCas9 H119 miniABEMax V82G | nSaCas9-ABEMax(V82G) H119 (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (H119 )
pcDNA3.1(+)
|
| nSaCas9 M732 miniABEMax V82G | nSaCas9 M732 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )
pcDNA3.1(+)
|
| nSaCas9 M741 miniABEMax V82G | nSaCas9 M741 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )
pcDNA3.1(+)
|
| nSaCas9 N120 miniABEMax V82G | nSaCas9 N120 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )
pcDNA3.1(+)
|
| nSaCas9 P742 miniABEMax V82G | nSaCas9 P742 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (P742 )
pcDNA3.1(+)
|
| nSaCas9 Q731 miniABEMax V82G | nSaCas9 Q731 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q731 )
pcDNA3.1(+)
|
| nSaCas9 Q737 miniABEMax V82G | nSaCas9 Q737 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q737 )
pcDNA3.1(+)
|
| nSaCas9 S740 miniABEMax V82G | nSaCas9 S740 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (S740 )
pcDNA3.1(+)
|
| nSaCas9 T128 miniABEMax V82G | nSaCas9 T128 miniABEMax V82G (Synthetic) | | Blasticidin |
Hewitt
| Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing.
Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
|
Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (T128 )
pcDNA3.1(+)
|
| SauriABEmax | | | |
Wang
| A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope.
PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar.
|
Expresses ABEmax and nSauriCas9
pFS84-CMV-pfs
|
| SauriBE4max | | | |
Wang
| A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope.
PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar.
|
Expresses AncBE4max and nSauriCas9
pFS84-CMV-pfs
|
| epi-ABEmax | | | Blasticidin |
Wang
| Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease.
Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020.
|
Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
epiCRISPR plasmid
|
| epi-BE4max | | | Blasticidin |
Wang
| Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease.
Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020.
|
Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
epiCRISPR plasmid
|
| epi-ABEmax-NG | | | Blasticidin |
Wang
| Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease.
Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020.
|
Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
epiCRISPR plasmid
|
| epi-BE4max-NG | | | Blasticidin |
Wang
| Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease.
Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020.
|
Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin
epiCRISPR plasmid
|
| PB-XL-AncBE4max | AncBE4max_GFP (Synthetic) | TRE3G | Blasticidin |
Ye
| Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing.
(2019) Nat Commun 10, 5353
|
Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max
XLone-GFP (Addgene plasmid no. 96930)
|
| pspCas9-ABE-3'UTR-sgRNA-ST2-com vector | ABE and sgRNA (Other) | | |
Lu
|
Lentiviral capsid delivery of SpCas9 RNP
(unpublished)
|
Expresses ABE and ST2-com modified sgRNA scaffold
pCDNA3.1
|
| ABE8.8-m | ABE8.8-m | | |
Gaudelli
| Directed evolution of adenine base editors with increased activity and therapeutic application.
Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.
|
expresses ABE8.8-m in mammalian cells
pBR322, beta-lactamase maintenance, CMV promoter
|
| ABE8.8-d | ABE8.8-d | | |
Gaudelli
| Directed evolution of adenine base editors with increased activity and therapeutic application.
Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.
|
expresses ABE8.8-d in mammalian cells
pBR322, beta-lactamase maintenance, CMV promoter
|
| ABE8.13-m | ABE8.13-m | | |
Gaudelli
| Directed evolution of adenine base editors with increased activity and therapeutic application.
Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.
|
expresses ABE8.13-m in mammalian cells
pBR322, beta-lactamase maintenance, CMV promoter
|
| ABE8.13-d | ABE8.13-d | | |
Gaudelli
| Directed evolution of adenine base editors with increased activity and therapeutic application.
Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.
|
expresses ABE8.13-d in mammalian cells
pBR322, beta-lactamase maintenance, CMV promoter
|
| ABE8.17-m | ABE8.17-m | | |
Gaudelli
| Directed evolution of adenine base editors with increased activity and therapeutic application.
Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.
|
expresses ABE8.17-m in mammalian cells
pBR322, beta-lactamase maintenance, CMV promoter
|
| ABE8.17-d | ABE8.20-d | | |
Gaudelli
| Directed evolution of adenine base editors with increased activity and therapeutic application.
Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.
|
expresses ABE8.17-d in mammalian cells
pBR322, beta-lactamase maintenance, CMV promoter
|
| ABE8.20-m
| ABE8.20-m | | |
Gaudelli
| Directed evolution of adenine base editors with increased activity and therapeutic application.
Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.
|
expresses ABE8.20-m in mammalian cells
pBR322, beta-lactamase maintenance, CMV promoter
|
| ABE8.20-d | ABE8.20-d | CMV | |
Gaudelli
| Directed evolution of adenine base editors with increased activity and therapeutic application.
Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.
|
expresses ABE8.20-d in mammalian cells
pBR322, beta-lactamase maintenance, CMV promoter
|
| U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA | St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic) | CAG, U6, CAG, CAG | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA
MSP1594_2x_NLS (Plasmid #110625)
APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
|
| U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA | St1Cas9 LMD9:CNRZ1066 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic) | CAG, U6, CAG, CAG | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA
MSP1594_2x_NLS (Plasmid #110625)
APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
|
| U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA | St1Cas9 LMD9:LMG18311 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic) | CAG, U6, CAG, CAG | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA
MSP1594_2x_NLS (Plasmid #110625)
APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
|
| U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA | St1Cas9 LMD9:TH1477 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic) | CAG, U6, CAG, CAG | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
MSP1594_2x_NLS (Plasmid #110625)
APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
|
| U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9 | St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), ABE7.10 (Homo sapiens) | CAG, U6, CAG | |
Doyon
| Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9.
Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.
|
Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA
MSP1594_2x_NLS (Plasmid #110625)
|
| pLenti-FNLSNG-P2A-Puro | FNLSNG (Synthetic) | EF1s | Puromycin |
Dow
| GO: a functional reporter system to identify and enrich base editing activity.
Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124.
|
Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)
Adapted from pLentiCRISPRv1
Tags
/ Fusion Proteins- FLAG-NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| pLenti-FNLSHF1-P2A-Puro | FNLSHF1 (Synthetic) | EF1s | Puromycin |
Dow
| GO: a functional reporter system to identify and enrich base editing activity.
Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124.
|
Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)
Adapted from pLentiCRISPRv1
Tags
/ Fusion Proteins- FLAG-NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| pLenti-FNLSHiFi-P2A-Puro | FNLSHiFi (Synthetic) | EF1s | Puromycin |
Dow
| GO: a functional reporter system to identify and enrich base editing activity.
Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124.
|
Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)
Adapted from pLentiCRISPRv1
Tags
/ Fusion Proteins- FLAG-NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| pLenti-FNLSHiFiNG-P2A-Puro | FNLSHiFiNG (Synthetic) | EF1s | Puromycin |
Dow
| GO: a functional reporter system to identify and enrich base editing activity.
Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124.
|
Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)
Adapted from pLentiCRISPRv1
Tags
/ Fusion Proteins- FLAG-NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| pCMV-BE4max-NRRH | BE4max-NRRH (Synthetic) | CMV | |
Liu
| Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Nat Biotechnology
|
Expresses BE4max-NRRH in mammalian cells
pBR322 CMV
|
| pCMV-BE4max-NRTH | BE4max-NRTH (Synthetic) | CMV | |
Liu
| Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Nat Biotechnology
|
Expresses BE4max-NRTH in mammalian cells
pBR322 CMV
|
| pCMV-BE4max-NRCH | BE4max-NRCH (Synthetic) | CMV | |
Liu
| Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Nat Biotechnology
|
Expresses BE4max-NRCH in mammalian cells
pBR322 CMV
|
| pCMV-ABEmax-NRTH | ABEmax-NRTH (Synthetic) | CMV | |
Liu
| Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Nat Biotechnology
|
Expresses ABEmax-NRTH in mammalian cells
pBR322 CMV
|
| pCMV-ABEmax-NRCH | ABEmax-NRCH (Synthetic) | CMV | |
Liu
| Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Nat Biotechnology
|
Expresses ABEmax-NRCH in mammalian cells
pBR322 CMV
|
| CMV_Npu-BE3.9max N-terminal | Npu-BE3.9max_N-terminal (Synthetic) | CMV | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter
pCMV
|
| CMV_Npu-BE3.9max C-terminal | Npu-BE3.9max_C-terminal (Synthetic) | CMV | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter
pCMV
|
| CMV_Npu-ABEmax N-terminal | Npu-ABEmax_N-terminal (Synthetic) | CMV | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter
pCMV
|
| CMV_Npu-ABEmax C-terminal | Npu-ABEmax_C-terminal (Synthetic) | CMV | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter
pCMV
|
| Cbh_v5 AAV-ABE N-terminal | v5 AAV-ABE N-terminal (Synthetic) | Cbh | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter
px601
|
| Cbh_v5 AAV-ABE C-terminal | v5 AAV-CBE C-terminal; U6-protospacer (Synthetic) | CMV | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA
px601
|
| CMV_Npu-saBE3.9max N-terminal | Npu-saBE3.9max_N-terminal (Synthetic) | CMV | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter
pCMV
|
| CMV_Npu-saBE3.9max C-terminal | Npu-saBE3.9max_C-terminal (Synthetic) | CMV | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter
pCMV
|
| CMV_Npu-saBE3.9max_KKH C-terminal | Npu-saBE3.9max_KKH_C-terminal (Synthetic) | CMV | |
Liu
| Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses.
Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
|
Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter
pCMV
|
| YE1-BE4max | rAPOBEC1(YE1)-nSpCas9-UGI-UGI (Other) | CMV | |
Liu
| Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.
|
C-to-T base editor
pBR322/CMV
Tags
/ Fusion Proteins- NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| YE2-BE4max | rAPOBEC1(YE2)-nSpCas9-UGI-UGI (Other) | CMV | |
Liu
| Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.
|
C-to-T base editor
pBR322/CMV
Tags
/ Fusion Proteins- NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| YEE-BE4max | rAPOBEC1(YEE)-nSpCas9-UGI-UGI (Other) | CMV | |
Liu
| Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.
|
C-to-T base editor
pBR322/CMV
Tags
/ Fusion Proteins- NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| EE-BE4max | rAPOBEC1(EE)-nSpCas9-UGI-UGI (Other) | CMV | |
Liu
| Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.
|
C-to-T base editor
pBR322/CMV
Tags
/ Fusion Proteins- NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| YE1-BE4max-NG | rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI (Other) | CMV | |
Liu
| Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.
|
C-to-T base editor
pBR322/CMV
Tags
/ Fusion Proteins- NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| YE1-BE4max-CP1028 | rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI (Other) | CMV | |
Liu
| Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.
|
C-to-T base editor
pBR322/CMV
Tags
/ Fusion Proteins- NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| AALN-BE4max | rAPOBEC1(AALN)-nSpCas9-UGI-UGI (Other) | CMV | |
Liu
| Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors.
Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.
|
C-to-T base editor
pBR322/CMV
Tags
/ Fusion Proteins- NLS (N terminal on insert)
- NLS (C terminal on insert)
|
| pEF-BE4Gam | BE4Gam (Synthetic) | Ef1a | |
Wang
| A transient reporter for editing enrichment (TREE) in human cells.
Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713.
|
Expression of BE4am-Cas9 Base-editor from an EF1a promoter.
pEF
|
| pEF-AncBE4max | AncBE4max (Synthetic) | Ef1a | |
Wang
| A transient reporter for editing enrichment (TREE) in human cells.
Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713.
|
Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter.
pEF
|
| pCMV-BE4-PpAPOBEC1 H122A | BE4-PpAPOBEC1 H122A (Synthetic) | CMV | |
Gaudelli
| Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Nat Commun 11, 2052 (2020)
|
Mammalian expression plasmid for BE4-PpABOBEC1H122A
pCMV
|
| pCMV-BE4-RrA3F | BE4-RrA3F (Synthetic) | CMV | |
Gaudelli
| Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Nat Commun 11, 2052 (2020)
|
Mammalian expression plasmid for BE4-RrA3F
pCMV
|
| pCMV-BE4-RrA3F F130L | BE4-RrA3F F130L (Synthetic) | CMV | |
Gaudelli
| Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Nat Commun 11, 2052 (2020)
|
Mammalian expression plasmid for BE4-RrA3F F130L
pCMV
|
| pCMV-BE4-AmAPOBEC1 | BE4-AmAPOBEC1 (Synthetic) | CMV | |
Gaudelli
| Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Nat Commun 11, 2052 (2020)
|
Mammalian expression plasmid for BE4-AmABOBEC1
pCMV
|
| pCMV-BE4-SsAPOBEC3B | BE4-SsAPOBEC3B (Synthetic) | CMV | |
Gaudelli
| Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Nat Commun 11, 2052 (2020)
|
Mammalian expression plasmid for BE4-SsAPOBEC3B
pCMV
|
| pCMV-BE4-SsAPOBEC3B R54Q | BE4-SsAPOBEC3B R54Q (Synthetic) | CMV | |
Gaudelli
| Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Nat Commun 11, 2052 (2020)
|
Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q
pCMV
|
| pCMV-BE4-PpAPOBEC1 R33A | BE4-PpAPOBEC1 R33A (Synthetic) | CMV | |
Gaudelli
| Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Nat Commun 11, 2052 (2020)
|
Mammalian expression plasmid for BE4-PpABOBEC1 R33A
pCMV
|
| pCMV-BE4-PpAPOBEC1 | BE4-PpAPOBEC1 (Synthetic) | CMV | |
Gaudelli
| Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Nat Commun 11, 2052 (2020)
|
Mammalian expression plasmid for BE4-PpABOBEC1
pCMV
|
| ABE8e | ecTadA(8e)-nSpCas9 (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| ABE8e-dimer | ecTadA(WT)-ecTadA(8e)-nSpCas9 (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| NG-ABE8e | ecTadA(8e)-nSpCas9 (NG) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| CP1028-ABE8e | ecTadA(8e)-nSpCas9 (CP1028) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| CP1041-ABE8e | ecTadA(8e)-nSpCas9 (CP1041) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| ABE8e(TadA-8e V82G) | ecTadA(8e V82G)-nSpCas9 (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| ABE8e(TadA-8e V106W) | ecTadA(8e V106W)-nSpCas9 (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| ABE8e(TadA-8e K20A R21A) | ecTadA(8e K20A R21A)-nSpCas9 (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| NG-ABE8-dimer | ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| CP1028-ABE8e-dimer | ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| CP1041-ABE8e-dimer | ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| SaABE8e | ecTadA(8e)-nSaCas9 (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| SaABE8e-dimer | ecTadA(WT)-ecTadA(8e)-nSaCas9 (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| SaKKH-ABE8e | ecTadA(8e)-nSaCas9 (KKH) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| SaKKH-ABE8e-dimer | ecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| LbABE8e | ecTadA(8e)-dLbCas12a (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| LbABE8e-dimer | ecTadA(WT)-ecTadA(8e)-dLbCas12a (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| enAsABE8e | ecTadA(8e)-dAsCas12a (En) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| enAsABE8e-dimer | ecTadA(WT)-ecTadA(8e)-dAsCas12a (En) (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| LbABE8e(TadA-8e V106W) | ecTadA(8e V106W)-dLbCas12a (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| SaABE8e(TadA V106W) | ecTadA(8e V106W)-nSaCas9 (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
A-to-G base editor
pCMV with a BR322 origin
|
| pBT44c | inteinC-dSpCas9-UGI (Other) | | |
Liu
| Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Nat Biotechnol (2020)
|
P2
pCMV with a BR322 origin
|
| H446 EF1a RA-ABE6.3 p2A Puro | ABE RA6.3 (Other) | EF1a | Puromycin |
Xue
| Adenine base editing in an adult mouse model of tyrosinaemia.
Nat Biomed Eng. 2019 Feb 25. pii: 10.1038/s41551-019-0357-8. doi: 10.1038/s41551-019-0357-8.
|
Lentiviral expression of ABE6.3.
lenti
|
| pCMV-Target-ACEmax | Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS (Synthetic) | CMV promoter | |
Yachie
| Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.
|
Mammalian Target-ACEmax expression vector (pSI-625)
pcDNA3.1
|
| pCMV-ACBEmax | Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS (Rattus norvegicus) | CMV promoter | |
Yachie
| Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.
|
Mammalian ACBEmax expression vector (pSI-632)
pcDNA3.1
|
| pCMV-Target-AIDmax | Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS (Synthetic) | CMV promoter | |
Yachie
| Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.
|
Mammalian Target-AIDmax expression vector (pSI-626)
pcDNA3.1
|
| pCAG-CBE4max-SpG-P2A-EGFP (RTW4552) | human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP (Synthetic) | CAG | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
pRZ33
Tag
/ Fusion Protein- P2A-EGFP (C terminal on insert)
|
| pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133) | human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP (Synthetic) | CAG | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
pRZ33
Tag
/ Fusion Protein- P2A-EGFP (C terminal on insert)
|
| pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637) | human codon optimized CBE4max xCas9(3.7) with P2A-EGFP (Synthetic) | CAG | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
pRZ33
Tag
/ Fusion Protein- P2A-EGFP (C terminal on insert)
|
| pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554) | human codon optimized CBE4max SpCas9-NG with P2A-EGFP (Synthetic) | CAG | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
pRZ33
Tag
/ Fusion Protein- P2A-EGFP (C terminal on insert)
|
| pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) | human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
pCMV_ABEmax_P2A_GFP
Tag
/ Fusion Protein- P2A-EGFP (C terminal on insert)
|
| pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) | human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
pCMV_ABEmax_P2A_GFP
Tag
/ Fusion Protein- P2A-EGFP (C terminal on insert)
|
| pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644) | human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
pCMV_ABEmax_P2A_GFP
Tag
/ Fusion Protein- P2A-EGFP (C terminal on insert)
|
| pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564) | human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP (Synthetic) | CMV and T7 | |
Kleinstiver
| Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.
|
CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
pCMV_ABEmax_P2A_GFP
Tag
/ Fusion Protein- P2A-EGFP (C terminal on insert)
|
| pCAG-BE4max-P2A-EGFP (pRZ33) | Human codon optimized pCAG BE4max with P2A-EGFP (Synthetic) | CAG | |
Joung
|
Joung Lab Plasmids
(unpublished)
|
CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP
pCAG backbone
|
| SPACE (pRZ1813) | SPACE (Other) | CMV | |
Joung
| A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.
|
CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP
CMV
|
| SPACE-ΔUGI (pRZ1836) | SPACE-ΔUGI (Other) | CMV | |
Joung
| A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.
|
CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP
CMV
|
| SPACE-NG (pRZ5150) | SPACE_NG (Other) | CMV | |
Joung
| A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.
|
CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP
CMV
|
| SPACE-VRQR (pRZ5137) | SPACE_VRQR (Other) | CMV | |
Joung
| A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.
|
CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP
CMV
|
| SECURE BE4max(R33A) (pBM608) | BE4max(R33A) (Rattus norvegicus) | CMV | |
Joung
| CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.
Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
|
CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP
CMV
|
| CGBE1 (pRZ3885) | eUNG-BE4max(R33A)-ΔUGI (Rattus norvegicus) | CMV | |
Joung
| CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.
Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
|
CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP
CMV
|
| miniCGBE1 (pBM1219) | BE4max(R33A)-ΔUGI (Rattus norvegicus) | CMV | |
Joung
| CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.
Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
|
CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP
CMV
|
| CGBE1-VRQR (pBM1192) | eUNG-BE4max(R33A)_VRQR-ΔUGI (Rattus norvegicus) | CMV | |
Joung
| CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.
Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
|
CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP
CMV
|
| miniCGBE1-VRQR (pBM1173) | BE4max(R33A)_VRQR-ΔUGI (Rattus norvegicus) | CMV | |
Joung
| CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.
Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
|
CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP
CMV
|
| CGBE1-NG (pBM1246) | eUNG-BE4max(R33A)_NG-ΔUGI (Rattus norvegicus) | CMV | |
Joung
| CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.
Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
|
CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
CMV
|
| miniCGBE1-NG (pBM1182) | BE4max(R33A)_NG-ΔUGI (Rattus norvegicus) | CMV | |
Joung
| CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells.
Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.
|
CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
CMV
|
| p2T-CMV-ABEmax-BlastR | ABE7.10-nSpCas9 (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
A-to-G base editor
p2T-CMV-BlastR
|
| p2T-CMV-ABEmaxCP1041-BlastR | ABE7.10-nSpCas9 CP1041 (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
A-to-G base editor
p2T-CMV-BlastR
|
| p2T-CMV-BE4max-BlastR | BE4-nSpCas9-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
C-to-T base editor
p2T-CMV-BlastR
|
| p2T-CMV-EA-BE4max-NG-BlastR | BE4 H47E+S48A-nSpCas9-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
High purity C-to-T base editor
p2T-CMV-BlastR
|
| p2T-CMV-BE4maxCP1028-BlastR | BE4-nSpCas9 CP1028-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
C-to-T base editor
p2T-CMV-BlastR
|
| p2T-CMV-AIDmax-BlastR | AID-nSpCas9-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
C-to-T base editor
p2T-CMV-BlastR
|
| p2T-CMV-CDAmax-BlastR | CDA-nSpCas9-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
C-to-T base editor
p2T-CMV-BlastR
|
| p2T-CMV-evoAmax-BlastR | evoA-nSpCas9-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
C-to-T base editor
p2T-CMV-BlastR
|
| p2T-CMV-eA3Amax-BlastR | eA3A-nSpCas9-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
C-to-T base editor
p2T-CMV-BlastR
|
| p2T-CMV-eA3A-BE5-NG-BlastR | eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
High purity C-to-T base editor
p2T-CMV-BlastR
|
| p2T-CMV-eA3AmaxNG-BlastR | eA3A-nSpCas9 NG-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
C-to-T base editor, NG PAM
p2T-CMV-BlastR
|
| p2T-CMV-eA3Amax-T31A-NG-BlastR | eA3A-BE4 T31A-nSpCas9-UGI-UGI (Synthetic) | CMV | |
Liu
| Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.
Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.
|
C-to-T/G/A base editor
p2T-CMV-BlastR
|
| CMV-YE1-BE3-FNLS-CMV-mCherry | YE1-BE3-FNLS (Synthetic) | CMV | |
Yang
| A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.
Nat Methods. 2020 May 18. pii: 10.1038/s41592-020-0832-x. doi: 10.1038/s41592-020-0832-x.
|
Express YE1-BE3-FNLS
unspecified
|
| G1333 DddAtox-N–dSpCas9 | bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS | | |
Liu
| A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.
|
expresses split DddAtox-Cas9 construct in mammalian cells
pCMV
|
| G1333 DddAtox-C–dSpCas9 | bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS | | |
Liu
| A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.
|
expresses split DddAtox-Cas9 construct in mammalian cells
pCMV
|
| G1397 DddAtox-N–dSpCas9 | bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS | | |
Liu
| A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.
|
expresses split DddAtox-Cas9 construct in mammalian cells
pCMV
|
| G1397 DddAtox-C–dSpCas9 | bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS | | |
Liu
| A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.
|
expresses split DddAtox-Cas9 construct in mammalian cells
pCMV
|
| G1333 DddAtox-N–SaKKH-Cas9(D10A) | G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS | | |
Liu
| A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.
|
expresses split DddAtox-Cas9 construct in mammalian cells
pCMV
|
| G1333 DddAtox-C–SaKKH-Cas9(D10A) | G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS | | |
Liu
| A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.
|
expresses split DddAtox-Cas9 construct in mammalian cells
pCMV
|
| G1397 DddAtox-N–SaKKH-Cas9(D10A) | G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS | | |
Liu
| A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.
|
expresses split DddAtox-Cas9 construct in mammalian cells
pCMV
|
| G1397 DddAtox-C–SaKKH-Cas9(D10A) | G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS | | |
Liu
| A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.
|
expresses split DddAtox-Cas9 construct in mammalian cells
pCMV
|
| Lenti-117G-hyA3A-BE4max | Lenti-117G- hyA3A-BE4max (Synthetic) | | |
Li
| Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain.
Nat Cell Biol. 2020 Jun;22(6):740-750. doi: 10.1038/s41556-020-0518-8. Epub 2020 May 11.
|
Lentiviral expression of hyA3A-BE4max
Adapted from lentiCRISPRv1
|
| Lenti-117G-hyeA3A-BE4max | Lenti -117G-hyeA3A-BE4max (Synthetic) | | |
Li
| Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain.
Nat Cell Biol. 2020 Jun;22(6):740-750. doi: 10.1038/s41556-020-0518-8. Epub 2020 May 11.
|
Lentiviral expression of hyeA3A-BE4max
Adapted from lentiCRISPRv1
|
| AID-BE4max-P2A-GFP | AID-BE4max (Synthetic) | | |
Li
| Dual base editor catalyzes both cytosine and adenine base conversions in human cells.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 10.1038/s41587-020-0527-y.
|
Mammalian expression of AID-BE4max-P2A-GFP
pCMV
|
| Lenti ABE7.10-N-AIDmax | Lenti ABE7.10-N-AIDmax (Synthetic) | | |
Li
| Dual base editor catalyzes both cytosine and adenine base conversions in human cells.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 10.1038/s41587-020-0527-y.
|
Lentiviral expression of ABE7.10-N-AIDmax
Adapted from lentiCRISPRv1
|
| Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP | Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP (Synthetic) | | |
Li
| Dual base editor catalyzes both cytosine and adenine base conversions in human cells.
Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 10.1038/s41587-020-0527-y.
|
Lentiviral expression of HBG site 1 sgRNA
Adapted from lentiCRISPRv2
|
| pCMV-ABE8eWQ | bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS (Homo sapiens) | | |
Bae
|
Bae lab plasmids
(unpublished)
|
Expresses ABE8eWQ in mammalian cells
pCMV
|
| pCMV-ABE-P48R | bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Homo sapiens) | | |
Bae
|
Bae lab plasmids
(unpublished)
|
Expresses ABE-P48R in mammalian cells
pCMV
|
| pCMV-ABE-P48R-UGI | bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS (Homo sapiens) | | |
Bae
|
Bae lab plasmids
(unpublished)
|
Expresses ABE-P48R-UGI in mammalian cells
pCMV
|
| pCMV-ABE7.10-P2A-GFP | ABE7.10-P2A-GFP (Synthetic) | CMV | |
Kim
|
Mammalian expression plasmids ABE7.10 with different promoters
(unpublished)
|
Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells
pCMV
|
| EFS-ABE7.10-P2A-GFP | ABE7.10-P2A-GFP (Synthetic) | EFS | |
Kim
|
Mammalian expression plasmids ABE7.10 with different promoters
(unpublished)
|
Express ABE7.10-P2A-GFP by EFS promoter in mammalian cells
pCMV
|
| hPGK-ABE7.10-P2A-GFP | ABE7.10-P2A-GFP (Synthetic) | hPGK | |
Kim
|
Mammalian expression plasmids ABE7.10 with different promoters
(unpublished)
|
Express ABE7.10-P2A-GFP by hPGK promoter in mammalian cells
pCMV
|
| pCMV_APOBEC1-UdgX-nCas9 | APOBEC1-UdgX-nCas9 (Homo sapiens) | | |
Liu
|
Liu Lab CGBE base editors
(unpublished)
|
Mammalian CG-to-GC base editing
pCMV
|
| pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX | POLD2-APOBEC1-UdgX-nCas9-UdgX (Homo sapiens) | | |
Liu
|
Liu Lab CGBE base editors
(unpublished)
|
Mammalian CG-to-GC base editing
pCMV
|
| pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX | POLD2-APOBEC1-UdgX-NG-nCas9-UdgX (Homo sapiens) | | |
Liu
|
Liu Lab CGBE base editors
(unpublished)
|
Mammalian CG-to-GC base editing
pCMV
|
| pCMV_UdgX-APOBEC1-UdgX-nCas9 | UdgX-APOBEC1-UdgX-nCas9 (Homo sapiens) | | |
Liu
|
Liu Lab CGBE base editors
(unpublished)
|
Mammalian CG-to-GC base editing
pCMV
|
| pCMV_UdgX-APOBEC1-UdgX-HF-nCas9 | UdgX-APOBEC1-UdgX-HF-nCas9 (Homo sapiens) | | |
Liu
|
Liu Lab CGBE base editors
(unpublished)
|
Mammalian CG-to-GC base editing
pCMV
|
| pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9 | UdgX-APOBEC1-UdgX-HF-NG-nCas9 (Homo sapiens) | | |
Liu
|
Liu Lab CGBE base editors
(unpublished)
|
Mammalian CG-to-GC base editing
pCMV
|
| pCMV_UdgX-APOBEC1-UdgX-NG-nCas9 | UdgX-APOBEC1-UdgX-NG-nCas9 (Homo sapiens) | | |
Liu
|
Liu Lab CGBE base editors
(unpublished)
|
Mammalian CG-to-GC base editing
pCMV
|
| A3G-BE4.4 | A3G-BE4.4 (Synthetic) | CMV | |
Gao
| Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects.
Sci Adv. 2020 Jul 15;6(29):eaba1773. doi: 10.1126/sciadv.aba1773. eCollection 2020 Jul.
|
C to T DNA base editing
pCMV
|