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CRISPR Plasmids: Base Edit

Browse CRISPR Plasmids

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CRISPR Resources

Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific base editor that can alter DNA bases without inducing a DNA break. Base editors convert C->T (or G->A on the opposite strand) within a small editing window specified by the gRNA. CRISPR-X, a base-editing system from the Bassik lab, uses an MS2 hairpin-tagged gRNA to recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed evolution. If you’re interested in making specific modifications to a gene rather than creating loss-of-function frameshift alleles, base editors may be useful in your research.

CRISPR base editing schematic


Browse, sort, or search the tables below for CRISPR base editing plasmids.
Plasmids are available for expression in mammalian systems, bacteria, plants, yeast, and zebrafish.

Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
pCMV-BE1BE1 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE1 with C-terminal NLS in mammalian cells CMV
pCMV-BE2BE2 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE2 in mammalian cells CMV
pCMV-BE3BE3 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE3 in mammalian cells CMV
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)SpCas9 (Other)pCMVNeomycin (select with G418) Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells pcDNA3.1
MO91-dCas9-AID(P182X)dCas9-AID P182X (Synthetic)MSCVPuromycin Chang Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10. Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone. M091
pJL-SaBE3SaBE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses SaBE3 in mammalian cells CMV
pJL-SaKKH-BE3SaKKH-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses SaKKH-BE3 in mammalian cells [Edit] CMV
pBK-VQR-BE3VQR-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses VQR-BE3 in mammalian cells CMV
pBK-EQR-BE3EQR-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses EQR-BE3 in mammalian cells CMV
pBK-VRER-BE3VRER-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses VRER-BE3 in mammalian cells CMV
pBK-YE1-BE3YE1-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YE1-BE3 in mammalian cells CMV
pBK-EE-BE3EE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses EE-BE3 in mammalian cells CMV
pBK-YE2-BE3YEE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YE2-BE3 in mammalian cells CMV
pBK-YEE-BE3YEE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YEE-BE3 in mammalian cells CMV
pGH335_MS2-AID*Δ-HygroMS2-AID*Δ and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker Addgene #61426
pGH183_MS2-AIDΔDead-HygroMS2-AIDΔDead and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker Addgene #61426
pGH156_MS2-AID-HygroMS2-AID and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker Addgene #61426
pGH153_MS2-AIDΔ-HygroMS2-AIDΔ and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker Addgene #61426
pCMV-HF-BE3HF-BE3 (Homo sapiens)CMV Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790. Expresses HF-BE3 with C-terminal NLS in mammalian cells pCMV
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLSrAPOBEC1-nCas9 (D10A)-UGI-NLS (Rattus norvegicus)T7 Kim Highly efficient RNA-guided base editing in mouse embryos. Nat Biotechnol. 2017 Feb 27. doi: 10.1038/nbt.3816. Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells. pET28a
BE4BE (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE4 in mammalian cells pCMV
AID-BE3activation induced cytidine deaminase (Homo sapiens)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses AID-BE3 in mammalian cells pCMV AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
CDA1-BE3CDA1-BE3 (Synthetic)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses CDA1-BE3 in mammalian cells pCMV
SaBE4SaBE4 (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE4 in mammalian cells pCMV
BE4-GamBE4-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE4-Gam in mammalian cells pCMV
BE3-GamBE3-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE3-Gam in mammalian cells pCMV
SaBE4-GamSaBE4-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE4-Gam in mammalian cells pCMV
SaBE3-GamSaBE3-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE3-Gam in mammalian cells pCMV
pCMV-eBE-S1eBE-S1 (Rattus norvegicus)CMV Chen Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. Expresses eBE-S1 in mammalian cells pCMV
pCMV-eBE-S3eBE-S3 (Rattus norvegicus)CMV Chen Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. Expresses eBE-S3 in mammalian cells pCMV
pCMV-ABE6.3ABE6.3 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE6.3 in mammalian cells pBR322/CMV
pCMV-ABE7.8ABE7.8 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.8 in mammalian cells pBR322/CMV
pCMV-ABE7.9ABE7.9 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.9 in mammalian cells pBR322/CMV
pCMV-ABE7.10ABE7.10 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.10 in mammalian cells pBR322/CMV
pCMV-dCpf1-BE0dLbCpf1-BE0 (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE0 in mammalian cells CMV
pCMV-dLbCpf1-BE0ΔiNLSdLbCpf1-BE0ΔiNLS (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells CMV
pCMV-dCpf1-BEdLbCpf1-BE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE in mammalian cells CMV
pCMV-dCpf1-BE-YEdLbCpf1-BE-YE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE-YE in mammalian cells CMV
pCMV-dCpf1-BE-YEEdLbCpf1-BE-YEE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE-YEE in mammalian cells CMV
pCMV-dCpf1-eBEdLbCpf1-eBE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-eBE in mammalian cells CMV
pCMV-dCpf1-eBE-YEdLbCpf1-eBE-YE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-eBE-YE in mammalian cells CMV
pCMV-ABEABE7.10 (Synthetic)CMV Huang Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7. For expression of ABE7.10 in mammalian cells PBR322
pWT063aTRESG-BE3 (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
pWT065a(TCF-LEF)7-BE3 (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
pWT065c(TCF-LEF)7-BE3-P2A-Luc (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
xCas9(3.7)-BE3xCas9(3.7)-BE3 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-BE3 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    xCas9(3.7)-BE4xCas9(3.7)-BE4 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-BE4 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    xCas9(3.7)-ABE(7.10)xCas9(3.7)-ABE(7.10) (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-ABE pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    xCas9(3.6)-BE3xCas9(3.6)-BE3 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-BE3 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    xCas9(3.6)-BE4xCas9(3.6)-BE4 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-BE4 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    xCas9(3.6)-ABE(7.10)xCas9(3.6)-ABE(7.10) (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-ABE pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    A3Ai-Cas9n-UGI-NLSApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3A
    A3Bi-ctd-Cas9n-UGI-NLSApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
    A3Bi-ctd E255A -Cas9n-UGI-NLSApolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
    A3Ai E72A-Cas9n-UGI-NLSApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-BE3-2XBE3-2X (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-2X construct (not codon optimized) pCMV
    pCMV-BE3-FNLSBE3-FNLS (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-FNLS construct (not codon optimized) pCMV
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
    		•
    pLenti-BE3-P2A-PuroBE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3 (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE3RA-P2A-PuroBE3RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-2X-P2A-PuroBE3RA-2X (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-FNLS-P2A-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
    		•
    pLenti-BE3-PGK-PuroBE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3 (not codon optimized) pLL3.3
    pLenti-BE3RA-PGK-PuroBE3RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) pLL3.3
    pLenti-FNLS-PGK-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) pLL3.3
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
    		•
    pLenti-TRE3G-BE3-PGK-PuroBE3 (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of BE3 (not codon optimized) pLL3.3
    pLenti-TRE3G-BE3RA-PGK-PuroBE3RA (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized) pLL3.3
    pLenti-TRE3G-FNLS-PGK-PuroBE3RA-FNLS (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized) pLL3.3
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
    		•
    pLenti-BE3-P2A-GFP-PGK-PuroBE3 (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE3RA-P2A-GFP-PGK-PuroBE3RA (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-FNLS-P2A-GFP-PGK-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
    		•
    pLenti-xBE3-P2A-PuroxCas9(3.7)-BE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells Adapted from lentiCRISPRv1
    pLenti-xFNLS-P2A-PuroxFNLS(3.7) (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
    		•
    pLenti-xF2X-P2A-PuroxFNLS-2X(3.7) (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
    		•
    pCMV_BE4maxBE4max (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_AncBE4maxAncBE4max (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_ABEmaxABEmax (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
    pCMV_BE4max_3xHABE4max_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
    		•
    pCMV_AncBE4max_3xHAAncBE4max_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
    		•
    pCMV_ABEmax_3xHAABEmax_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
    		•
    pCMV_BE4max_P2A_GFPBE4max_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_AncBE4max_P2A_GFPAncBE4max_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_ABEmax_P2A_GFPABEmax_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
    pLL1 sgRNA(MS2)-MS2-AIDmonoAID mono mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AIDmono pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL2 sgRNA(MS2)-MS2-AID3cAID mono 3c mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AID3c pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL3 sgRNA(MS2)-MS2-AID3fAIDmono 3f mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AID3f pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL4 sgRNA(MS2)-MS2-hA3ahuman Apobec3a (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pLL5 sgRNA(MS2)-MS2-hA3a D131Ahuman Apobec3a D131A mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a D131A pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pLL6 sgRNA(MS2)-MS2-hA3a D131Ehuman Apobec3a D131E mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a D131E pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-BE3-FNLS(RA)BE3-FNLS (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-FNLS construct (codon optimized) pCMV
    pLenti-BE4RA-P2A-PuroBE4RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE4GamRA-P2A-PuroBE4GamRA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-ABE7.10-P2A-PuroABE7.10 (Synthetic)EF1sPuromycin Dow Dow Lab Unpublished Base Editors (unpublished) Lentivirus for constitutive expression of ABE7.10 (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-ABERA-P2A-PuroABERA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of ABE7.10 RA (codon optimized) Adapted from lentiCRISPRv1
    pLenti-xBE4Gam-P2A-PuroxCas9(3.7)-BE4Gam (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pAAV-ABE-NT-sgRNAABE-NT-sgRNA (Other) Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA pAAV
    rat APOBEC1-EGFPapolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Rattus norvegicus)CMV promoterNeomycin (select with G418) Conticello Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells. RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033. plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein pEGFP-N1 Apobec1 REPR, apobec-1
    pEGFP mCherry-ApoB-EGFPmCherry-apoB-EGFP (Synthetic)CMV promoterNeomycin (select with G418) Conticello Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells. RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033. Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP pEGFP-N1
    pAAV-ABE-CTABE-CT Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. AAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9 pAAV
    pST1374-scFv-APOBEC-UGI-GB1scFv-rAPOBEC1-UGI-GB1 (Rattus norvegicus)CMV Huang BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4. Expresses scFv-rAPOBEC1-UGI in mammalian cells pST1374
    p3s-ABE7.10ABE 7.10 (Other)CMV Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized pCDNA3.1
    pCMV-hA3A-BE3hA3A-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3 in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3B-BE3hA3B-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3B-BE3 in mammalian cells CMV APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
    pCMV-hA3C-BE3hA3C-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3C-BE3 in mammalian cells CMV APOBEC3C A3C, APOBEC1L, ARDC2, ARDC4, ARP5, PBI, bK150C2.3
    pCMV-hA3D-BE3hA3D-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3D-BE3 in mammalian cells CMV APOBEC3D A3D, APOBEC3DE, APOBEC3E, ARP6
    pCMV-hA3F-BE3hA3F-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3F-BE3 in mammalian cells CMV APOBEC3F A3F, ARP8, BK150C2.4.MRNA, KA6
    pCMV-hA3G-BE3hA3G-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3G-BE3 in mammalian cells CMV APOBEC3G A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1
    pCMV-hA3H-BE3hA3H-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3H-BE3 in mammalian cells CMV APOBEC3H A3H, ARP-10, ARP10
    pCMV-hAID-BE3hAID-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hAID-BE3 in mammalian cells CMV AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pCMV-hA1-BE3hA1-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA1-BE3 in mammalian cells CMV APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    pCMV-mA3-BE3mA3-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mA3-BE3 in mammalian cells CMV Apobec3 Apobec, Arp3, BC003314, Cem15, Gm20117, Rfv3
    pCMV-mAID-BE3mAID-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mAID-BE3 in mammalian cells CMV Aicda Aid, Arp2
    pCMV-mA1-BE3mA1-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mA1-BE3 in mammalian cells CMV Apobec1 Cdar1
    pCMV-cAICDA-BE3cAICDA-BE3 (Other)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses cAICDA-BE3 in mammalian cells CMV aicda aid
    pCMV-hA3A-BE3-D131YhA3A-BE3-D131Y (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-D131Y in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-Y132DhA3A-BE3-Y132D (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-Y132D in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-C101ShA3A-BE3-C101S (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-C101S in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-C106ShA3A-BE3-C106S (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-C106S in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-Y130FhA3A-BE3-Y130F (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-Y130F in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized AsBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized AsBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
    		•
    pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
    		•
    pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.3 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
    		•
    pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized LbBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
    		•
    pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4) (Synthetic)CAG Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized LbBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
    		•
    PB-BE3-NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    PB-BE3-2NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    PB-BE3-3NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    A3Bi-Cas9n-UGIAPOBEC3B L1 intron-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction BE3
    A3C-Cas9n-UGIAPOBEC3C-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3C BE3
    A3D-Cas9n-UGIAPOBEC3D-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3D BE3
    A3F-Cas9n-UGIAPOBEC3F-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3F BE3
    A3G-Cas9n-UGIAPOBEC3G-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3G BE3
    A3H HapI-Cas9n-UGIAPOBEC3H Haplotype I-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype I BE3
    A3H HapII-Cas9n-UGIAPOBEC3H Haplotype II-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype II BE3
    A3H HapII R175/6E-Cas9n-UGIAPOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE BE3
    CP1012-CBEmaxrApobec1-nSpCas9 (CP1012) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1028-CBEmaxrApobec1-nSpCas9 (CP1028) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1249-CBEmaxrApobec1-nSpCas9 (CP1249) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1300-CBEmaxrApobec1-nSpCas9 (CP1300) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1012-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1012) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1028-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1028) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1041-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1041) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1249-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1249) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1300-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1300) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    VRQR-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (VRQR) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    VRER-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (VRER) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    xABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (xCas9) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    SaABEmaxecTadA-ecTadA*(7.10)-nSaCas9 (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    pSI-Target-AID-NGnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) Nureki Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129. Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells. pcDNA3.1
    pLV302N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE) (Rattus norvegicus)CMV Schwank Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8. Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells px601
    pLV312.3C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3 (Rattus norvegicus)CMV Schwank Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8. Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells px601
    pcDNA3.1(-)-HF2-BE2HF2-BE2 (Synthetic)CMVNeomycin (select with G418) Huang Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5. Expresses HF2-BE2 in mammalian cells pcDNA3.1(-)
    pcDNA3.1(-)-HF2-BE3HF2-BE3 (Synthetic)CMVNeomycin (select with G418) Huang Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5. Expresses HF2-BE3 in mammalian cells pcDNA3.1(-)
    pcDNA3.1(-)-ABE7.10ABE7.10 (Synthetic)CMVNeomycin (select with G418) Huang Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z. Expresses ABE7.10 in mammalian cells pcDNA3.1(-)
    CAG-FNLS-T2A-EGFP-ires-puroFNLS-T2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance. PyCAG
    CAG-ABEmax-P2A-EGFP-ires-puroABEmax-P2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance. PyCAG
    CAG-xFNLS-T2A-EGFP-ires-puroxFNLS-T2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance. PyCAG
    pBT44c intein-dCas9-ugi fusion (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. BE PACE selection, express C-intein-Cas9-ugi fusion pBT38
    pBT276 CDA1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express CDA1-BE4max in mammalian cells pBT195
    pBT277 evoCDA1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express evoCDA1-BE4max in mammalian cells pBT195
    pBT281 evoAPOBEC1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express evoAPOBEC1-BE4max in mammalian cells pBT195
    nCas9-UGI-NLS-P2A-EGFP (pJUL1001)nCas9-UGI-NLS-P2A-EGFP (Synthetic)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1). CAG
    BE3-P2A-EGFP (pJUL977)BE3-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP. CAG
    BE3(E63Q)-P2A-EGFP (pRZ189)BE3(E63Q)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant). CAG
    SECURE BE3(R33A)-P2A-EGFP (pJUL1085)BE3(R33A)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A). CAG
    SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)BE3(R33A/K34A)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A). CAG
    NG-ABEmaxTadA-TadA(evo)-Cas9-NG Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV
    dABEdABE7.10 (Other) Church Enabling large-scale genome editing by reducing DNA nicking bioRxiv Expresses dead Cas9 (dCas9)-ABE7.10 pCMV_ABE7.10
    dCBE4-gamdCBE4-gam (Other) Church Enabling large-scale genome editing by reducing DNA nicking bioRxiv Expresses dead Cas9 (dCas9)-CBE4-gam pCMV

    Bacteria

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication
    pET42b-BE3BE3 (Homo sapiens)T7 Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
    pET42b-HF-BE3HF-BE3 (Homo sapiens) Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
    pET28b-BE3∆UGIBE3∆UGI (Rattus norvegicus)T7 Kim Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nat Biotechnol. 2017 May;35(5):475-480. doi: 10.1038/nbt.3852. Epub 2017 Apr 10.
    pScI_dCas9-CDAdCas9-PmCDA1 (Synthetic)lambda OR Kondo Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
    pScI_dCas9-CDA_J23119-sgRNAdCas9-PmCDA1 (Synthetic), Streptococcus pyogenes sgRNA (Synthetic)lambda OR, J23119 Kondo Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
    pScI_dCas9-CDA-ULdCas9-PmCDA1-UGI-LVA (Synthetic)lambda OR Kondo Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
    pnCasPA-BEC Ji CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species. iScience. 2018 Aug 31;6:222-231. doi: 10.1016/j.isci.2018.07.024. Epub 2018 Aug 1.
    pBECKP-kmncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase (Other) Ji Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
    pBECKP-spencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase (Other) Ji Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
    pET42b-A3A-PBE-ΔUGIAPOBEC3A-nCas9-NLS (Other)T7 promoter Gao Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
    pET42b-ABE7.10ABE7.10 (Synthetic)T7 Huang Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.
    pBECAb-apr Ji Plasmids for genome editing in Acinetobacter baumannii (unpublished)

    Plant

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pMonAID_dCas9-PmCDA_Hyg_ALSSpCas9 (Other)2x35SHygromycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
    pMonAID_nCas9-PmCDA_Hyg_ALSSpCas9 (Other)2x35SHygromycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
    pDicAID_nCas9-PmCDA_NptII_DellaSpCas9 (Other)PcUbiKanamycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella pZD_OsU3gYSA_HolgerCas9_NPTII
    pDicAID_nCas9-PmCDA-2A-NptII_ETRSpCas9 (Other)PcUbiKanamycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr pZD_OsU3gYSA_HolgerCas9_NPTII
    pHEE901Hygromycin Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR pCambia
    pHSE901Hygromycin Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR pCambia
    pBSE901Neomycin (select with G418) Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance pCambia
    pXSE901BBasta Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance pCambia
    pXSE901BGBasta ; Glyphosate Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance pCambia
    pB-dCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbiBasta Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in maize pBUE411
    pB-nCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbiBasta Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in maize pBUE411
    pdCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbi Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
    pH-dCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbiHygromycin Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411
    pH-nCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbiHygromycin Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411
    pnCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbi Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
    pJY-RpABERPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA cassetteHygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for ABE expression in Arabidopsis pKI1.1R
    pJY-RpABE-FT_INFRPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting FTHygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis pJY-RpABE
    pJY-RpABE-PDS3_IMSRPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting PDS3Hygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis pJY-RpABE
    pH-PABE-7-esgRNAwtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in rice pCAMBIA1300
    pH-PABE-7-sgRNAwtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in rice pCAMBIA1300
    PABE-1NLS-wtTadA-TadA7.10-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-2wtTadA-TadA7.10-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-3wtTadA-TadA7.10-NLS-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-4NLS-nCas9-wtTadA-TadA7.10-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-5NLS-nCas9-NLS-wtTadA-TadA7.10-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-6wtTadA-TadA7.10-nCas9-2*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-7wtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    pYPQ255rAPOBEC1-pcoCas9D10A-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing pNJB91
    pYPQ255-x3.7rAPOBEC1-pcoCas9D10A_x3.7-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
    pYPQ256pcoCas9D10A-PmCDA1-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing pNJB91
    pYPQ256-x3.7pcoCas9D10A_x3.7-PmCDA1-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
    pFH45nSpCas9-ABE7.10 base changer, human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants bioRxiv Level0 nSpCas9-ABE7.10 base changer, human codon optimized pICH41308
    pFH55nSpCas9-PmCDA, Arabidopsis codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants bioRxiv Level0 nSpCas9-PmCDA, Arabidopsis codon optimized pICH41308
    pFH92nScCas9-ABE7.10, wheat/human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants bioRxiv Level0 nScCas9-ABE7.10, wheat/human codon optimized pICH41308
    pFH70ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants bioRxiv Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt pICH47742
    pFH84ZmUBIp::A3A-PBE (wheat codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants bioRxiv Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt pICH47742
    pFH93ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants bioRxiv Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt pICH47742

    Yeast

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHLSpCas9 (Other), PmCDA1 (Other)pGal1, pGal10LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells pRS315
    pRS315e_pGal-dCas9-PmCDA1SpCas9 (Other)pGal1LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses dCas9-PmCDA1 in yeast cells pRS315
    pRS315e_pGal-nCas9(D10A)-PmCDA1SpCas9 (Other)pGal1LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9(D10A)-PmCDA1 in yeast cells pRS315

    Zebrafish

    ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    101739pCMV-BE-zCas9rAPOBEC1-XTEN-zCas9-UGI-NLS (Rattus norvegicus)CMV Lin Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. expression of an optimized Cas9 for base-editing in zebrafish CMV
    101740pCMV-BE-VQRCas9rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS (Rattus norvegicus)CMV Lin Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. expression of an optimized Cas9 for base-editing in zebrafish CMV


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