CRISPR
Base Edit

CRISPR Plasmids: Base Edit

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Two classes of base editors--cytosine base editors (CBEs) and adenine base editors (ABEs)--can be used to generate single base pair edits without double stranded breaks.

Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand.)

Likewise, adenine base editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor.

Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by optimizing expression of the fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage Mu- derived Gam protein (Mu- GAM).

While many base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution applications. Examples of these base editing systems include targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab, in which Cas9 is fused to activation-induced cytidine deaminase (AID).

Browse, sort, or search the tables below for CRISPR base editing plasmids.
Plasmids are available for expression in mammalian systems, bacteria, plants, yeast, and zebrafish.

Mammalian

Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
pCMV-BE1	BE1 (Rattus norvegicus)	CMV		Liu	Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946.	Expresses BE1 with C-terminal NLS in mammalian cells CMV
pCMV-BE2	BE2 (Rattus norvegicus)	CMV		Liu	Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946.	Expresses BE2 in mammalian cells CMV
pCMV-BE3	BE3 (Rattus norvegicus)	CMV		Liu	Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946.	Expresses BE3 in mammalian cells CMV
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)	SpCas9 (Other)	pCMV	Neomycin (select with G418)	Kondo	Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729.	Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells pcDNA3.1
MO91-dCas9-AID(P182X)	dCas9-AID P182X (Synthetic)	MSCV	Puromycin	Chang	Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10.	Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone. M091
pJL-SaBE3	SaBE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses SaBE3 in mammalian cells CMV
pJL-SaKKH-BE3	SaKKH-BE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses SaKKH-BE3 in mammalian cells [Edit] CMV
pBK-VQR-BE3	VQR-BE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses VQR-BE3 in mammalian cells CMV
pBK-EQR-BE3	EQR-BE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses EQR-BE3 in mammalian cells CMV
pBK-VRER-BE3	VRER-BE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses VRER-BE3 in mammalian cells CMV
pBK-YE1-BE3	YE1-BE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses YE1-BE3 in mammalian cells CMV
pBK-EE-BE3	EE-BE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses EE-BE3 in mammalian cells CMV
pBK-YE2-BE3	YEE-BE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses YE2-BE3 in mammalian cells CMV
pBK-YEE-BE3	YEE-BE3 (Rattus norvegicus)	CMV		Liu	Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803.	Expresses YEE-BE3 in mammalian cells CMV
pGH335_MS2-AID*Δ-Hygro	MS2-AID*Δ and Hygromycin resistance		Hygromycin	Bassik	Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.	lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker Addgene #61426
pGH183_MS2-AIDΔDead-Hygro	MS2-AIDΔDead and Hygromycin resistance		Hygromycin	Bassik	Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.	lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker Addgene #61426
pGH156_MS2-AID-Hygro	MS2-AID and Hygromycin resistance		Hygromycin	Bassik	Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.	lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker Addgene #61426
pGH153_MS2-AIDΔ-Hygro	MS2-AIDΔ and Hygromycin resistance		Hygromycin	Bassik	Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.	lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker Addgene #61426
pCMV-HF-BE3	HF-BE3 (Homo sapiens)	CMV		Liu	Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.	Expresses HF-BE3 with C-terminal NLS in mammalian cells pCMV
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS	rAPOBEC1-nCas9 (D10A)-UGI-NLS (Rattus norvegicus)	T7		Kim	Highly efficient RNA-guided base editing in mouse embryos. Nat Biotechnol. 2017 Feb 27. doi: 10.1038/nbt.3816.	Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells. pET28a
BE4	BE (Rattus norvegicus)	CMV		Liu	Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.	Expresses BE4 in mammalian cells pCMV
AID-BE3	activation induced cytidine deaminase (Homo sapiens)	CMV		Liu	Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.	Expresses AID-BE3 in mammalian cells pCMV AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
CDA1-BE3	CDA1-BE3 (Synthetic)	CMV		Liu	Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.	Expresses CDA1-BE3 in mammalian cells pCMV
SaBE4	SaBE4 (Rattus norvegicus)	CMV		Liu	Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.	Expresses SaBE4 in mammalian cells pCMV
BE4-Gam	BE4-Gam (Rattus norvegicus)	CMV		Liu	Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.	Expresses BE4-Gam in mammalian cells pCMV
BE3-Gam	BE3-Gam (Rattus norvegicus)	CMV		Liu	Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.	Expresses BE3-Gam in mammalian cells pCMV
SaBE4-Gam	SaBE4-Gam (Rattus norvegicus)	CMV		Liu	Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.	Expresses SaBE4-Gam in mammalian cells pCMV
SaBE3-Gam	SaBE3-Gam (Rattus norvegicus)	CMV		Liu	Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug.	Expresses SaBE3-Gam in mammalian cells pCMV
pCMV-eBE-S1	eBE-S1 (Rattus norvegicus)	CMV		Chen	Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111.	Expresses eBE-S1 in mammalian cells pCMV
pCMV-eBE-S3	eBE-S3 (Rattus norvegicus)	CMV		Chen	Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111.	Expresses eBE-S3 in mammalian cells pCMV
pCMV-ABE6.3	ABE6.3 (Synthetic)	CMV		Liu	Programmable base editing of AT to GC in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.	expresses ABE6.3 in mammalian cells pBR322/CMV
pCMV-ABE7.8	ABE7.8 (Synthetic)	CMV		Liu	Programmable base editing of AT to GC in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.	expresses ABE7.8 in mammalian cells pBR322/CMV
pCMV-ABE7.9	ABE7.9 (Synthetic)	CMV		Liu	Programmable base editing of AT to GC in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.	expresses ABE7.9 in mammalian cells pBR322/CMV
pCMV-ABE7.10	ABE7.10 (Synthetic)	CMV		Liu	Programmable base editing of AT to GC in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25.	expresses ABE7.10 in mammalian cells pBR322/CMV
pCMV-dCpf1-BE0	dLbCpf1-BE0 (Rattus norvegicus)	CMV		Chen	Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.	Expresses dLbCpf1-BE0 in mammalian cells CMV
pCMV-dLbCpf1-BE0ΔiNLS	dLbCpf1-BE0ΔiNLS (Rattus norvegicus)	CMV		Chen	Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.	Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells CMV
pCMV-dCpf1-BE	dLbCpf1-BE (Rattus norvegicus)	CMV		Chen	Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.	Expresses dLbCpf1-BE in mammalian cells CMV
pCMV-dCpf1-BE-YE	dLbCpf1-BE-YE (Rattus norvegicus)	CMV		Chen	Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.	Expresses dLbCpf1-BE-YE in mammalian cells CMV
pCMV-dCpf1-BE-YEE	dLbCpf1-BE-YEE (Rattus norvegicus)	CMV		Chen	Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.	Expresses dLbCpf1-BE-YEE in mammalian cells CMV
pCMV-dCpf1-eBE	dLbCpf1-eBE (Rattus norvegicus)	CMV		Chen	Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.	Expresses dLbCpf1-eBE in mammalian cells CMV
pCMV-dCpf1-eBE-YE	dLbCpf1-eBE-YE (Rattus norvegicus)	CMV		Chen	Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.	Expresses dLbCpf1-eBE-YE in mammalian cells CMV
pCMV-ABE	ABE7.10 (Synthetic)	CMV		Huang	Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7.	For expression of ABE7.10 in mammalian cells PBR322
pWT063a	TRESG-BE3 (Synthetic)			Liu	Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992.	W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
pWT065a	(TCF-LEF)7-BE3 (Synthetic)			Liu	Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992.	W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
pWT065c	(TCF-LEF)7-BE3-P2A-Luc (Synthetic)			Liu	Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992.	W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
xCas9(3.7)-BE3	xCas9(3.7)-BE3 (Synthetic)			Liu	Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.	Mammalian expression vector for xCas9(3.7)-BE3 pCMV Tag / Fusion Protein NLS (N terminal on insert)
xCas9(3.7)-BE4	xCas9(3.7)-BE4 (Synthetic)			Liu	Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.	Mammalian expression vector for xCas9(3.7)-BE4 pCMV Tag / Fusion Protein NLS (N terminal on insert)
xCas9(3.7)-ABE(7.10)	xCas9(3.7)-ABE(7.10) (Synthetic)			Liu	Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.	Mammalian expression vector for xCas9(3.7)-ABE pCMV Tag / Fusion Protein NLS (N terminal on insert)
xCas9(3.6)-BE3	xCas9(3.6)-BE3 (Synthetic)			Liu	Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.	Mammalian expression vector for xCas9(3.6)-BE3 pCMV Tag / Fusion Protein NLS (N terminal on insert)
xCas9(3.6)-BE4	xCas9(3.6)-BE4 (Synthetic)			Liu	Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.	Mammalian expression vector for xCas9(3.6)-BE4 pCMV Tag / Fusion Protein NLS (N terminal on insert)
xCas9(3.6)-ABE(7.10)	xCas9(3.6)-ABE(7.10) (Synthetic)			Liu	Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155.	Mammalian expression vector for xCas9(3.6)-ABE pCMV Tag / Fusion Protein NLS (N terminal on insert)
A3Ai-Cas9n-UGI-NLS	Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (Homo sapiens)	CMV		Harris	A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.	Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3A
A3Bi-ctd-Cas9n-UGI-NLS	Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (Homo sapiens)	CMV		Harris	A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.	Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
A3Bi-ctd E255A -Cas9n-UGI-NLS	Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (Homo sapiens)	CMV		Harris	A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.	Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
A3Ai E72A-Cas9n-UGI-NLS	Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (Homo sapiens)	CMV		Harris	A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332.	Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pCMV-BE3-2X	BE3-2X (Synthetic)	CMV		Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	CMV expression vector for BE3-2X construct (not codon optimized) pCMV
pCMV-BE3-FNLS	BE3-FNLS (Synthetic)	CMV		Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	CMV expression vector for BE3-FNLS construct (not codon optimized) pCMV Tag / Fusion Protein 3X FLAG (N terminal on insert)
pLenti-BE3-P2A-Puro	BE3 (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of BE3 (not codon optimized) Adapted from lentiCRISPRv1
pLenti-BE3RA-P2A-Puro	BE3RA (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
pLenti-2X-P2A-Puro	BE3RA-2X (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
pLenti-FNLS-P2A-Puro	BE3RA-FNLS (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1 Tag / Fusion Protein 3X FLAG (N terminal on insert)
pLenti-BE3-PGK-Puro	BE3 (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of BE3 (not codon optimized) pLL3.3
pLenti-BE3RA-PGK-Puro	BE3RA (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) pLL3.3
pLenti-FNLS-PGK-Puro	BE3RA-FNLS (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) pLL3.3 Tag / Fusion Protein 3X FLAG (N terminal on insert)
pLenti-TRE3G-BE3-PGK-Puro	BE3 (Synthetic)	TRE3G	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for dox-inducible expression of BE3 (not codon optimized) pLL3.3
pLenti-TRE3G-BE3RA-PGK-Puro	BE3RA (Synthetic)	TRE3G	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized) pLL3.3
pLenti-TRE3G-FNLS-PGK-Puro	BE3RA-FNLS (Synthetic)	TRE3G	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized) pLL3.3 Tag / Fusion Protein 3X FLAG (N terminal on insert)
pLenti-BE3-P2A-GFP-PGK-Puro	BE3 (Synthetic)	EF1s	Puromycin ; GFP	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized) Adapted from lentiCRISPRv1
pLenti-BE3RA-P2A-GFP-PGK-Puro	BE3RA (Synthetic)	EF1s	Puromycin ; GFP	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
pLenti-FNLS-P2A-GFP-PGK-Puro	BE3RA-FNLS (Synthetic)	EF1s	Puromycin ; GFP	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1 Tag / Fusion Protein 3X FLAG (N terminal on insert)
pLenti-xBE3-P2A-Puro	xCas9(3.7)-BE3 (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells Adapted from lentiCRISPRv1
pLenti-xFNLS-P2A-Puro	xFNLS(3.7) (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1 Tag / Fusion Protein 3X FLAG (N terminal on insert)
pLenti-xF2X-P2A-Puro	xFNLS-2X(3.7) (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1 Tag / Fusion Protein 3X FLAG (N terminal on insert)
pCMV_BE4max	BE4max (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	C:G-to-T:A base editing pCMV
pCMV_AncBE4max	AncBE4max (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	C:G-to-T:A base editing pCMV
pCMV_ABEmax	ABEmax (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	A:T-to-G:C base editing pCMV
pCMV_BE4max_3xHA	BE4max_3xHA (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	C:G-to-T:A base editing pCMV Tag / Fusion Protein 3xHA (C terminal on insert)
pCMV_AncBE4max_3xHA	AncBE4max_3xHA (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	C:G-to-T:A base editing pCMV Tag / Fusion Protein 3xHA (C terminal on insert)
pCMV_ABEmax_3xHA	ABEmax_3xHA (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	A:T-to-G:C base editing pCMV Tag / Fusion Protein 3xHA (C terminal on insert)
pCMV_BE4max_P2A_GFP	BE4max_P2A_GFP (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	C:G-to-T:A base editing pCMV
pCMV_AncBE4max_P2A_GFP	AncBE4max_P2A_GFP (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	C:G-to-T:A base editing pCMV
pCMV_ABEmax_P2A_GFP	ABEmax_P2A_GFP (Homo sapiens)	CMV		Liu	Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.	A:T-to-G:C base editing pCMV
pLL1 sgRNA(MS2)-MS2-AIDmono	AID mono mutant (Homo sapiens)	hU6,CBh		Meng	Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.	empty sgRNA cloning vector with MS2-AIDmono pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
pLL2 sgRNA(MS2)-MS2-AID3c	AID mono 3c mutant (Homo sapiens)	hU6,CBh		Meng	Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.	empty sgRNA cloning vector with MS2-AID3c pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
pLL3 sgRNA(MS2)-MS2-AID3f	AIDmono 3f mutant (Homo sapiens)	hU6,CBh		Meng	Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.	empty sgRNA cloning vector with MS2-AID3f pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
pLL4 sgRNA(MS2)-MS2-hA3a	human Apobec3a (Homo sapiens)	hU6,CBh		Meng	Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.	empty sgRNA cloning vector with MS2-humanA3a pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pLL5 sgRNA(MS2)-MS2-hA3a D131A	human Apobec3a D131A mutant (Homo sapiens)	hU6,CBh		Meng	Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.	empty sgRNA cloning vector with MS2-humanA3a D131A pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pLL6 sgRNA(MS2)-MS2-hA3a D131E	human Apobec3a D131E mutant (Homo sapiens)	hU6,CBh		Meng	Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090.	empty sgRNA cloning vector with MS2-humanA3a D131E pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pCMV-BE3-FNLS(RA)	BE3-FNLS (Synthetic)	CMV		Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	CMV expression vector for BE3-FNLS construct (codon optimized) pCMV
pLenti-BE4RA-P2A-Puro	BE4RA (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
pLenti-BE4GamRA-P2A-Puro	BE4GamRA (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
pLenti-ABE7.10-P2A-Puro	ABE7.10 (Synthetic)	EF1s	Puromycin	Dow	Dow Lab Unpublished Base Editors (unpublished)	Lentivirus for constitutive expression of ABE7.10 (not codon optimized) Adapted from lentiCRISPRv1
pLenti-ABERA-P2A-Puro	ABERA (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentivirus for constitutive expression of ABE7.10 RA (codon optimized) Adapted from lentiCRISPRv1
pLenti-xBE4Gam-P2A-Puro	xCas9(3.7)-BE4Gam (Synthetic)	EF1s	Puromycin	Dow	Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194.	Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
pAAV-ABE-NT-sgRNA	ABE-NT-sgRNA (Other)			Kim	Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27.	AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA pAAV
rat APOBEC1-EGFP	apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Rattus norvegicus)	CMV promoter	Neomycin (select with G418)	Conticello	Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells. RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033.	plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein pEGFP-N1 Apobec1 REPR, apobec-1
pEGFP mCherry-ApoB-EGFP	mCherry-apoB-EGFP (Synthetic)	CMV promoter	Neomycin (select with G418)	Conticello	Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells. RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033.	Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP pEGFP-N1
pAAV-ABE-CT	ABE-CT			Kim	Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27.	AAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9 pAAV
pST1374-scFv-APOBEC-UGI-GB1	scFv-rAPOBEC1-UGI-GB1 (Rattus norvegicus)	CMV		Huang	BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4.	Expresses scFv-rAPOBEC1-UGI in mammalian cells pST1374
p3s-ABE7.10	ABE 7.10 (Other)	CMV		Kim	Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27.	expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized pCDNA3.1
pCMV-hA3A-BE3	hA3A-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3A-BE3 in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pCMV-hA3B-BE3	hA3B-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3B-BE3 in mammalian cells CMV APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
pCMV-hA3C-BE3	hA3C-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3C-BE3 in mammalian cells CMV APOBEC3C A3C, APOBEC1L, ARDC2, ARDC4, ARP5, PBI, bK150C2.3
pCMV-hA3D-BE3	hA3D-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3D-BE3 in mammalian cells CMV APOBEC3D A3D, APOBEC3DE, APOBEC3E, ARP6
pCMV-hA3F-BE3	hA3F-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3F-BE3 in mammalian cells CMV APOBEC3F A3F, ARP8, BK150C2.4.MRNA, KA6
pCMV-hA3G-BE3	hA3G-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3G-BE3 in mammalian cells CMV APOBEC3G A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1
pCMV-hA3H-BE3	hA3H-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3H-BE3 in mammalian cells CMV APOBEC3H A3H, ARP-10, ARP10
pCMV-hAID-BE3	hAID-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hAID-BE3 in mammalian cells CMV AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
pCMV-hA1-BE3	hA1-BE3 (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA1-BE3 in mammalian cells CMV APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
pCMV-mA3-BE3	mA3-BE3 (Mus musculus)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses mA3-BE3 in mammalian cells CMV Apobec3 Apobec, Arp3, BC003314, Cem15, Gm20117, Rf, Rfv, Rfv3
pCMV-mAID-BE3	mAID-BE3 (Mus musculus)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses mAID-BE3 in mammalian cells CMV Aicda A, Aid, Arp2
pCMV-mA1-BE3	mA1-BE3 (Mus musculus)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses mA1-BE3 in mammalian cells CMV Apobec1 Cdar1
pCMV-cAICDA-BE3	cAICDA-BE3 (Other)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses cAICDA-BE3 in mammalian cells CMV aicda aid
pCMV-hA3A-BE3-D131Y	hA3A-BE3-D131Y (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3A-BE3-D131Y in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pCMV-hA3A-BE3-Y132D	hA3A-BE3-Y132D (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3A-BE3-Y132D in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pCMV-hA3A-BE3-C101S	hA3A-BE3-C101S (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3A-BE3-C101S in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pCMV-hA3A-BE3-C106S	hA3A-BE3-C106S (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3A-BE3-C106S in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pCMV-hA3A-BE3-Y130F	hA3A-BE3-Y130F (Homo sapiens)	CMV		Chen	Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018	Expresses hA3A-BE3-Y130F in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)	human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1) (Synthetic)			Joung	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.	CAG promoter expression plasmid for human codon optimized AsBE1.1 pCAG-CFP Tag / Fusion Protein SV40 NLS (C terminal on insert)
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)	human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4) (Synthetic)			Joung	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.	CAG promoter expression plasmid for human codon optimized AsBE1.4 pCAG-CFP Tags / Fusion Proteins 2x SV40 NLS (N terminal on insert) SV40 NLS (C terminal on insert)
pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)	DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1) (Synthetic)			Joung	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.	CAG promoter expression plasmid for human codon optimized enAsBE1.1 pCAG-CFP Tag / Fusion Protein SV40 NLS (C terminal on insert)
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)	DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2) (Synthetic)			Joung	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.	CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348 pCAG-CFP Tags / Fusion Proteins 2x SV40 NLS (N terminal on insert) SV40 NLS (C terminal on insert)
pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)	DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3) (Synthetic)			Joung	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.	CAG promoter expression plasmid for human codon optimized enAsBE1.3 pCAG-CFP Tag / Fusion Protein SV40 NLS (C terminal on insert)
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)	DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4) (Synthetic)			Joung	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.	CAG promoter expression plasmid for human codon optimized enAsBE1.4 pCAG-CFP Tags / Fusion Proteins 2x SV40 NLS (N terminal on insert) SV40 NLS (C terminal on insert)
pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)	human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1) (Synthetic)			Joung	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.	CAG promoter expression plasmid for human codon optimized LbBE1.1 pCAG-CFP Tag / Fusion Protein SV40 NLS (C terminal on insert)
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)	human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4) (Synthetic)	CAG		Joung	Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0.	CAG promoter expression plasmid for human codon optimized LbBE1.4 pCAG-CFP Tags / Fusion Proteins 2x SV40 NLS (N terminal on insert) SV40 NLS (C terminal on insert)
PB-BE3-NLS	BE3 (Other)			Li	CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4.	Stably express BE3 in mammalian cells PiggyBac-mRFP
PB-BE3-2NLS	BE3 (Other)			Li	CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4.	Stably express BE3 in mammalian cells PiggyBac-mRFP
PB-BE3-3NLS	BE3 (Other)			Li	CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4.	Stably express BE3 in mammalian cells PiggyBac-mRFP
A3Bi-Cas9n-UGI	APOBEC3B L1 intron-Cas9 nickase-UGI (Homo sapiens)	CMV		Harris	A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.	Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction BE3
A3C-Cas9n-UGI	APOBEC3C-Cas9 nickase-UGI (Homo sapiens)	CMV		Harris	A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.	Base editor made from APOBEC3C BE3
A3D-Cas9n-UGI	APOBEC3D-Cas9 nickase-UGI (Homo sapiens)	CMV		Harris	A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.	Base editor made from APOBEC3D BE3
A3F-Cas9n-UGI	APOBEC3F-Cas9 nickase-UGI (Homo sapiens)	CMV		Harris	A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.	Base editor made from APOBEC3F BE3
A3G-Cas9n-UGI	APOBEC3G-Cas9 nickase-UGI (Homo sapiens)	CMV		Harris	A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.	Base editor made from APOBEC3G BE3
A3H HapI-Cas9n-UGI	APOBEC3H Haplotype I-Cas9 nickase-UGI (Homo sapiens)	CMV		Harris	A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.	Base editor made from APOBEC3H Haplotype I BE3
A3H HapII-Cas9n-UGI	APOBEC3H Haplotype II-Cas9 nickase-UGI (Homo sapiens)	CMV		Harris	A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.	Base editor made from APOBEC3H Haplotype II BE3
A3H HapII R175/6E-Cas9n-UGI	APOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI (Homo sapiens)	CMV		Harris	A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.	Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE BE3
CP1012-CBEmax	rApobec1-nSpCas9 (CP1012) -UGI-UGI (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	C-to-T base editor pCMV with a BR322 origin
CP1028-CBEmax	rApobec1-nSpCas9 (CP1028) -UGI-UGI (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	C-to-T base editor pCMV with a BR322 origin
CP1041-CBEmax	rApobec1-nSpCas9 (CP1041) -UGI-UGI (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	C-to-T base editor pCMV with a BR322 origin
CP1249-CBEmax	rApobec1-nSpCas9 (CP1249) -UGI-UGI (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	C-to-T base editor pCMV with a BR322 origin
CP1300-CBEmax	rApobec1-nSpCas9 (CP1300) -UGI-UGI (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	C-to-T base editor pCMV with a BR322 origin
CP1012-ABEmax	ecTadA-ecTadA*(7.10)-nSpCas9 (CP1012) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
CP1028-ABEmax	ecTadA-ecTadA*(7.10)-nSpCas9 (CP1028) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
CP1041-ABEmax	ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
CP1249-ABEmax	ecTadA-ecTadA*(7.10)-nSpCas9 (CP1249) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
CP1300-ABEmax	ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
VRQR-ABEmax	ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
VRER-ABEmax	ecTadA-ecTadA*(7.10)-nSpCas9 (VRER) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
xABEmax	ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
SaABEmax	ecTadA-ecTadA*(7.10)-nSaCas9 (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
SaKKH-ABEmax	ecTadA-ecTadA*(7.10)-nSaCas9 (KKH) (Other)			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV with a BR322 origin
pSI-Target-AID-NG	nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)			Nureki	Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129.	Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells. pcDNA3.1
pLV302	N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE) (Rattus norvegicus)	CMV		Schwank	Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8.	Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells px601
pLV312.3	C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3 (Rattus norvegicus)	CMV		Schwank	Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8.	Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells px601
pcDNA3.1(-)-HF2-BE2	HF2-BE2 (Synthetic)	CMV	Neomycin (select with G418)	Huang	Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5.	Expresses HF2-BE2 in mammalian cells pcDNA3.1(-)
pcDNA3.1(-)-HF2-BE3	HF2-BE3 (Synthetic)	CMV	Neomycin (select with G418)	Huang	Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5.	Expresses HF2-BE3 in mammalian cells pcDNA3.1(-)
pcDNA3.1(-)-ABE7.10	ABE7.10 (Synthetic)	CMV	Neomycin (select with G418)	Huang	Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.	Expresses ABE7.10 in mammalian cells pcDNA3.1(-)
CAG-FNLS-T2A-EGFP-ires-puro	FNLS-T2A-EGFP-ires-puro (Other)		Puromycin ; EGFP	Otonkoski	Otonkoski plasmids 2018 (unpublished)	CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance. PyCAG
CAG-ABEmax-P2A-EGFP-ires-puro	ABEmax-P2A-EGFP-ires-puro (Other)		Puromycin ; EGFP	Otonkoski	Otonkoski plasmids 2018 (unpublished)	CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance. PyCAG
CAG-xFNLS-T2A-EGFP-ires-puro	xFNLS-T2A-EGFP-ires-puro (Other)		Puromycin ; EGFP	Otonkoski	Otonkoski plasmids 2018 (unpublished)	CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance. PyCAG
pBT44c	intein-dCas9-ugi fusion (Other)			Liu	Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.	BE PACE selection, express C-intein-Cas9-ugi fusion pBT38
pBT276	CDA1-BE4max (Other)			Liu	Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.	express CDA1-BE4max in mammalian cells pBT195
pBT277	evoCDA1-BE4max (Other)			Liu	Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.	express evoCDA1-BE4max in mammalian cells pBT195
pBT281	evoAPOBEC1-BE4max (Other)			Liu	Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.	express evoAPOBEC1-BE4max in mammalian cells pBT195
nCas9-UGI-NLS-P2A-EGFP (pJUL1001)	nCas9-UGI-NLS-P2A-EGFP (Synthetic)	CAG		Joung	Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.	CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1). CAG
BE3-P2A-EGFP (pJUL977)	BE3-P2A-EGFP (Rattus norvegicus)	CAG		Joung	Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.	CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP. CAG
BE3(E63Q)-P2A-EGFP (pRZ189)	BE3(E63Q)-P2A-EGFP (Rattus norvegicus)	CAG		Joung	Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.	CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant). CAG
SECURE BE3(R33A)-P2A-EGFP (pJUL1085)	BE3(R33A)-P2A-EGFP (Rattus norvegicus)	CAG		Joung	Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.	CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A). CAG
SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)	BE3(R33A/K34A)-P2A-EGFP (Rattus norvegicus)	CAG		Joung	Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z.	CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A). CAG
NG-ABEmax	TadA-TadA(evo)-Cas9-NG			Liu	Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y.	A-to-G base editor pCMV
dABE	dABE7.10 (Other)			Church	Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239.	Expresses dead Cas9 (dCas9)-ABE7.10 pCMV_ABE7.10
dCBE4-gam	dCBE4-gam (Other)			Church	Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239.	Expresses dead Cas9 (dCas9)-CBE4-gam pCMV
pBT371	CDA1-BE4max-NG (Other)			Liu	Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.	Expresses CDA1-BE4max-NG in mammalian cells pBT195
pBT372	evoCDA1-BE4max-NG (Other)			Liu	Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.	Expresses evoCDA1-BE4max-NG in mammalian cells pBT195
pBT375	evoAPOBEC1-BE4max-NG (Other)			Liu	Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0.	Expresses evoAPOBEC1-BE4max-NG in mammalian cells pBT195
pCMV-ABEmaxAW	ABEmax(TadA E59A, TadA* V106W) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	Expresses ABEmaxAW in mammalian cells CMV
pCMV-ABEmax(TadA E59A)	ABEmax(TadA E59A) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	Expresses ABEmax(TadA E59A) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*E59A)	ABEmax(TadA E59A TadA* E59A) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*V106M)	ABEmax(TadA E59A TadA* V106M) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*V106M) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*V106Q)	ABEmax(TadA E59A TadA* V106Q) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*V106Q) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*V106F)	ABEmax(TadA E59A TadA* V106F) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*V106F) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*D108W)	ABEmax(TadA E59A TadA* D108W) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*D108W) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*D108F)	ABEmax(TadA E59A TadA* D108F) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*D108F) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*D108Q)	ABEmax(TadA E59A TadA* D108Q) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*D108M)	ABEmax(TadA E59A TadA* D108M) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*D108M) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*R47Q)	ABEmax(TadA E59A TadA* R47Q) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*R47Q) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*R47F)	ABEmax(TadA E59A TadA* D108F) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*R47F) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*R47W)	ABEmax(TadA E59A TadA* R47W) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*R47W) in mammalian cells CMV
pCMV-ABEmax(TadA E59A, TadA*R47M)	ABEmax(TadA E59A TadA* R47M) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA E59A, TadA*R47M) in mammalian cells CMV
pCMV-ABEmax(TadA, TadA*E59A)	ABEmax(TadA E59A TadA* E59A) (Homo sapiens)			Liu	Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May.	pCMV-ABEmax(TadA, TadA*E59A) in mammalian cells CMV
pRS0045	TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI (Synthetic)	CMV promoter		Yachie	Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.	Mammalian Target-ACE expression vector pcDNA3.1
pRS0035	nCas9-PmCDA1-UGI (Synthetic)	CMV promoter		Yachie	Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.	Mammalian Target-AID expression vector pcDNA3.1
nCas9-UGI control for Target-AID (pRZ1047)	NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Other)	CAG		Joung	CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.	CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1). CAG
Target-AID (pRZ762)	NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP (Other)	CAG		Joung	CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.	CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP. CAG
miniABEmax (pRZ900)	bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)	CMV		Joung	CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.	CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain). CMV
SECURE miniABEmax-K20A/R21A (pJUL1774)	bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)	CMV		Joung	CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.	CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations). CMV
SECURE miniABEmax-V82G (pJUL1828)	bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)	CMV		Joung	CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.	CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation). CMV
hA3A-BE3 (pRZ215)	hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)	CAG		Joung	CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.	CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
eA3A-BE3 (pRZ206)	hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)	CAG		Joung	CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.	CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
hAID-BE3 (pRZ352)	hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)	CAG		Joung	CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6.	CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
BE3-YE1	BE3-(W90Y-R126E) (Synthetic)			Yang	Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10.	Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid. X330-U6-Chimeric_BB-CBh-hSpCas9
BE3-hA3A-R128A	BE3-hA3A(R128A) (Synthetic)			Yang	Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10.	Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid. X330-U6-Chimeric_BB-CBh-hSpCas9
BE3-hA3A-Y130F	BE3-hA3A(Y130F) (Synthetic)			Yang	Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10.	Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid. X330-U6-Chimeric_BB-CBh-hSpCas9
ABE7.10-F148A	ABE7.10(F148A) (Synthetic)			Yang	Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature. 2019 Jul;571(7764):275-278. doi: 10.1038/s41586-019-1314-0. Epub 2019 Jun 10.	Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid. X330-U6-Chimeric_BB-CBh-hSpCas9
N-4-BE	LjCDA1L1_4 (Other)	U6 with EF-1alpha intron		Cheng	Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.	C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9 Px330
N-8-BE	LpCDA1L1_1 (Other)	U6 with EF-1alpha intron		Cheng	Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.	C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9 Px330
C-1-BE	PmCDA1L1_4 (Other)	U6 with EF-1alpha intron		Cheng	Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.	C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9 Px330
C-7-BE	LpCDA1L1_1 (Other)	U6 with EF-1alpha intron		Cheng	Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.	C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9 Px330
C-11-BE	LpCDA1L2_2 (Other)	U6 with EF-1alpha intron		Cheng	Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.	C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9 Px330
C-12-BE	LjCDA1L2_1 (Other)	U6 with EF-1alpha intron		Cheng	Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.	C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9 Px330
C-13-BE	LpCDA1L1_4 (Other)	U6 with EF-1alpha intron		Cheng	Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.	C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9 Px330
AIDx-ID-1058	nSpyCas9-hAIDx-ID-1058-UGI (Homo sapiens)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus pcDNA3.1(+)
BE3-NLS-NLS-P2A-BLAST	BE3-2XNLS (Rattus norvegicus)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance pcDNA3.1(+)
S2 1058 AIDx no UGI	nSpyCas9-hAIDx-ID-1058 (Homo sapiens)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor. pcDNA3.1(+)
S2 1058 APOBEC3 no UGI	nSpyCas9-rAPOBEC3-ID-1058 (Rattus norvegicus)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor. pcDNA3.1(+)
S1 1029 CP APOBEC3 no UGI	rAPOBEC3-nSpyCas9 aa 1029 (Rattus norvegicus)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus pcDNA3.1(+)
S1 1029 AIDx with UGI	hAIDx-nSpyCas9 aa-UGI (Rattus norvegicus)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus pcDNA3.1(+)
pCMV-ABEMAX-NLS-P2A_BLAST	ABEMax-2XNLS (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance pcDNA3.1(+)
S2 1058 ABEMax	nSpyCas9-ABEMax aa 1058 (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9 pcDNA3.1(+)
S1 1029 CP ABEMax strategy 1	ABEMax-nSpyCas9 aa 1029 (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus pcDNA3.1(+)
S1 1029 CP ABEMax strategy 2	WT Tada-nSpyCas9 aa 1029-evolved Tada (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus) pcDNA3.1(+)
S1 1029 CP ABEMax strategy 3	1029 CP nSpyCas9 ABEMax (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus pcDNA3.1(+)
S1 1029 miniABEMax V82G strategy1	mini ABEMax 1029 nSpyCas9 (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus pcDNA3.1(+)
S2 1058 V82G ABEMax	1058 nSpyCas9 V82G ABEMax (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9 pcDNA3.1(+)
miniABEMax V82G wt	miniABEMax nSpyCas9 (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSpyCas9 with N-terminal miniABEMax (V82G) pcDNA3.1(+)
S1 1029 V82G ABEMax Strategy 3	1029 V82G ABEMax (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus pcDNA3.1(+)
ABEMax nSaCas9	ABEMax nSaCas9 (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus pcDNA3.1(+)
miniABEMax V82G nSaCas9	miniABEMax V82G nSaCas9 (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution pcDNA3.1(+)
nSaCas9 D127 miniABEMax V82G	nSaCas9 D127 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D127 ) pcDNA3.1(+)
nSaCas9 D126 miniABEMax V82G	nSaCas9 D126 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D126 ) pcDNA3.1(+)
nSaCas9 E739 miniABEMax V82G	nSaCas9 E739 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E739 ) pcDNA3.1(+)
nSaCas9 E743 miniABEMax V82G	nSaCas9 E743 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E743 ) pcDNA3.1(+)
nSaCas9 E745 miniABEMax V82G	nSaCas9 E745 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E745 ) pcDNA3.1(+)
nSaCas9 F733 miniABEMax V82G	nSaCas9 F733 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (F733 ) pcDNA3.1(+)
nSaCas9 N730 miniABEMax V82G	nSaCas9 N730 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N730 ) pcDNA3.1(+)
nSaCas9 E125 miniABEMax V82G	nSaCas9 E125 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E125 ) pcDNA3.1(+)
nSaCas9 E131 miniABEMax V82G	nSaCas9 E131 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E131 ) pcDNA3.1(+)
nSaCas9 L132 miniABEMax V82G	nSaCas9 L132 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (L132 ) pcDNA3.1(+)
nSaCas9 N130 miniABEMax V82G	nSaCas9-ABEMax(V82G) N130 (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N130 ) pcDNA3.1(+)
nSaCas9 E735 miniABEMax V82G	nSaCas9 E735 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E735 ) pcDNA3.1(+)
nSaCas9 I744 miniABEMax V82G	nSaCas9 I744 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 ) pcDNA3.1(+)
nSaCas9 G129 miniABEMax V82G	nSaCas9 G129 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (G129 ) pcDNA3.1(+)
nSaCas9 H119 miniABEMax V82G	nSaCas9-ABEMax(V82G) H119 (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (H119 ) pcDNA3.1(+)
nSaCas9 M732 miniABEMax V82G	nSaCas9 M732 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 ) pcDNA3.1(+)
nSaCas9 M741 miniABEMax V82G	nSaCas9 M741 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 ) pcDNA3.1(+)
nSaCas9 N120 miniABEMax V82G	nSaCas9 N120 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 ) pcDNA3.1(+)
nSaCas9 P742 miniABEMax V82G	nSaCas9 P742 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (P742 ) pcDNA3.1(+)
nSaCas9 Q731 miniABEMax V82G	nSaCas9 Q731 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q731 ) pcDNA3.1(+)
nSaCas9 Q737 miniABEMax V82G	nSaCas9 Q737 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q737 ) pcDNA3.1(+)
nSaCas9 S740 miniABEMax V82G	nSaCas9 S740 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (S740 ) pcDNA3.1(+)
nSaCas9 T128 miniABEMax V82G	nSaCas9 T128 miniABEMax V82G (Synthetic)		Blasticidin	Hewitt	Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing. Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.	Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (T128 ) pcDNA3.1(+)
SauriABEmax				Wang	A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar.	Expresses ABEmax and nSauriCas9 pFS84-CMV-pfs
SauriBE4max				Wang	A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar.	Expresses AncBE4max and nSauriCas9 pFS84-CMV-pfs
epi-ABEmax			Blasticidin	Wang	Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease. Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020.	Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
epi-BE4max			Blasticidin	Wang	Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease. Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020.	Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
epi-ABEmax-NG			Blasticidin	Wang	Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease. Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020.	Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
epi-BE4max-NG			Blasticidin	Wang	Base Editing Mediated Generation of Point Mutations Into Human Pluripotent Stem Cells for Modeling Disease. Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 10.3389/fcell.2020.590581. eCollection 2020.	Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
PB-XL-AncBE4max	AncBE4max_GFP (Synthetic)	TRE3G	Blasticidin	Ye	Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing. (2019) Nat Commun 10, 5353	Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max XLone-GFP (Addgene plasmid no. 96930)
pspCas9-ABE-3'UTR-sgRNA-ST2-com vector	ABE and sgRNA (Other)			Lu	Lentiviral capsid delivery of SpCas9 RNP (unpublished)	Expresses ABE and ST2-com modified sgRNA scaffold pCDNA3.1
ABE8.8-m	ABE8.8-m			Gaudelli	Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.	expresses ABE8.8-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
ABE8.8-d	ABE8.8-d			Gaudelli	Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.	expresses ABE8.8-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
ABE8.13-m	ABE8.13-m			Gaudelli	Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.	expresses ABE8.13-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
ABE8.13-d	ABE8.13-d			Gaudelli	Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.	expresses ABE8.13-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
ABE8.17-m	ABE8.17-m			Gaudelli	Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.	expresses ABE8.17-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
ABE8.17-d	ABE8.20-d			Gaudelli	Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.	expresses ABE8.17-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
ABE8.20-m	ABE8.20-m			Gaudelli	Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.	expresses ABE8.20-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
ABE8.20-d	ABE8.20-d	CMV		Gaudelli	Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6.	expresses ABE8.20-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA	St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)	CAG, U6, CAG, CAG		Doyon	Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.	Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA	St1Cas9 LMD9:CNRZ1066 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)	CAG, U6, CAG, CAG		Doyon	Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.	Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA	St1Cas9 LMD9:LMG18311 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)	CAG, U6, CAG, CAG		Doyon	Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.	Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA	St1Cas9 LMD9:TH1477 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)	CAG, U6, CAG, CAG		Doyon	Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.	Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9	St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), ABE7.10 (Homo sapiens)	CAG, U6, CAG		Doyon	Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3.	Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625)
pLenti-FNLSNG-P2A-Puro	FNLSNG (Synthetic)	EF1s	Puromycin	Dow	GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124.	Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1 Tags / Fusion Proteins FLAG-NLS (N terminal on insert) NLS (C terminal on insert)
pLenti-FNLSHF1-P2A-Puro	FNLSHF1 (Synthetic)	EF1s	Puromycin	Dow	GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124.	Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1 Tags / Fusion Proteins FLAG-NLS (N terminal on insert) NLS (C terminal on insert)
pLenti-FNLSHiFi-P2A-Puro	FNLSHiFi (Synthetic)	EF1s	Puromycin	Dow	GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124.	Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1 Tags / Fusion Proteins FLAG-NLS (N terminal on insert) NLS (C terminal on insert)
pLenti-FNLSHiFiNG-P2A-Puro	FNLSHiFiNG (Synthetic)	EF1s	Puromycin	Dow	GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124.	Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1 Tags / Fusion Proteins FLAG-NLS (N terminal on insert) NLS (C terminal on insert)
pCMV-BE4max-NRRH	BE4max-NRRH (Synthetic)	CMV		Liu	Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology	Expresses BE4max-NRRH in mammalian cells pBR322 CMV
pCMV-BE4max-NRTH	BE4max-NRTH (Synthetic)	CMV		Liu	Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology	Expresses BE4max-NRTH in mammalian cells pBR322 CMV
pCMV-BE4max-NRCH	BE4max-NRCH (Synthetic)	CMV		Liu	Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology	Expresses BE4max-NRCH in mammalian cells pBR322 CMV
pCMV-ABEmax-NRTH	ABEmax-NRTH (Synthetic)	CMV		Liu	Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology	Expresses ABEmax-NRTH in mammalian cells pBR322 CMV
pCMV-ABEmax-NRCH	ABEmax-NRCH (Synthetic)	CMV		Liu	Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology	Expresses ABEmax-NRCH in mammalian cells pBR322 CMV
CMV_Npu-BE3.9max N-terminal	Npu-BE3.9max_N-terminal (Synthetic)	CMV		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter pCMV
CMV_Npu-BE3.9max C-terminal	Npu-BE3.9max_C-terminal (Synthetic)	CMV		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter pCMV
CMV_Npu-ABEmax N-terminal	Npu-ABEmax_N-terminal (Synthetic)	CMV		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter pCMV
CMV_Npu-ABEmax C-terminal	Npu-ABEmax_C-terminal (Synthetic)	CMV		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter pCMV
Cbh_v5 AAV-ABE N-terminal	v5 AAV-ABE N-terminal (Synthetic)	Cbh		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter px601
Cbh_v5 AAV-ABE C-terminal	v5 AAV-CBE C-terminal; U6-protospacer (Synthetic)	CMV		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA px601
CMV_Npu-saBE3.9max N-terminal	Npu-saBE3.9max_N-terminal (Synthetic)	CMV		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter pCMV
CMV_Npu-saBE3.9max C-terminal	Npu-saBE3.9max_C-terminal (Synthetic)	CMV		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter pCMV
CMV_Npu-saBE3.9max_KKH C-terminal	Npu-saBE3.9max_KKH_C-terminal (Synthetic)	CMV		Liu	Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14.	Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter pCMV
YE1-BE4max	rAPOBEC1(YE1)-nSpCas9-UGI-UGI (Other)	CMV		Liu	Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.	C-to-T base editor pBR322/CMV Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
YE2-BE4max	rAPOBEC1(YE2)-nSpCas9-UGI-UGI (Other)	CMV		Liu	Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.	C-to-T base editor pBR322/CMV Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
YEE-BE4max	rAPOBEC1(YEE)-nSpCas9-UGI-UGI (Other)	CMV		Liu	Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.	C-to-T base editor pBR322/CMV Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
EE-BE4max	rAPOBEC1(EE)-nSpCas9-UGI-UGI (Other)	CMV		Liu	Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.	C-to-T base editor pBR322/CMV Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
YE1-BE4max-NG	rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI (Other)	CMV		Liu	Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.	C-to-T base editor pBR322/CMV Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
YE1-BE4max-CP1028	rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI (Other)	CMV		Liu	Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.	C-to-T base editor pBR322/CMV Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
AALN-BE4max	rAPOBEC1(AALN)-nSpCas9-UGI-UGI (Other)	CMV		Liu	Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6.	C-to-T base editor pBR322/CMV Tags / Fusion Proteins NLS (N terminal on insert) NLS (C terminal on insert)
pEF-BE4Gam	BE4Gam (Synthetic)	Ef1a		Wang	A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713.	Expression of BE4am-Cas9 Base-editor from an EF1a promoter. pEF
pEF-AncBE4max	AncBE4max (Synthetic)	Ef1a		Wang	A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713.	Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter. pEF
pCMV-BE4-PpAPOBEC1 H122A	BE4-PpAPOBEC1 H122A (Synthetic)	CMV		Gaudelli	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020)	Mammalian expression plasmid for BE4-PpABOBEC1H122A pCMV
pCMV-BE4-RrA3F	BE4-RrA3F (Synthetic)	CMV		Gaudelli	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020)	Mammalian expression plasmid for BE4-RrA3F pCMV
pCMV-BE4-RrA3F F130L	BE4-RrA3F F130L (Synthetic)	CMV		Gaudelli	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020)	Mammalian expression plasmid for BE4-RrA3F F130L pCMV
pCMV-BE4-AmAPOBEC1	BE4-AmAPOBEC1 (Synthetic)	CMV		Gaudelli	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020)	Mammalian expression plasmid for BE4-AmABOBEC1 pCMV
pCMV-BE4-SsAPOBEC3B	BE4-SsAPOBEC3B (Synthetic)	CMV		Gaudelli	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020)	Mammalian expression plasmid for BE4-SsAPOBEC3B pCMV
pCMV-BE4-SsAPOBEC3B R54Q	BE4-SsAPOBEC3B R54Q (Synthetic)	CMV		Gaudelli	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020)	Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q pCMV
pCMV-BE4-PpAPOBEC1 R33A	BE4-PpAPOBEC1 R33A (Synthetic)	CMV		Gaudelli	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020)	Mammalian expression plasmid for BE4-PpABOBEC1 R33A pCMV
pCMV-BE4-PpAPOBEC1	BE4-PpAPOBEC1 (Synthetic)	CMV		Gaudelli	Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020)	Mammalian expression plasmid for BE4-PpABOBEC1 pCMV
ABE8e	ecTadA(8e)-nSpCas9 (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
ABE8e-dimer	ecTadA(WT)-ecTadA(8e)-nSpCas9 (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
NG-ABE8e	ecTadA(8e)-nSpCas9 (NG) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
CP1028-ABE8e	ecTadA(8e)-nSpCas9 (CP1028) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
CP1041-ABE8e	ecTadA(8e)-nSpCas9 (CP1041) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
ABE8e(TadA-8e V82G)	ecTadA(8e V82G)-nSpCas9 (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
ABE8e(TadA-8e V106W)	ecTadA(8e V106W)-nSpCas9 (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
ABE8e(TadA-8e K20A R21A)	ecTadA(8e K20A R21A)-nSpCas9 (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
NG-ABE8-dimer	ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
CP1028-ABE8e-dimer	ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
CP1041-ABE8e-dimer	ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
SaABE8e	ecTadA(8e)-nSaCas9 (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
SaABE8e-dimer	ecTadA(WT)-ecTadA(8e)-nSaCas9 (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
SaKKH-ABE8e	ecTadA(8e)-nSaCas9 (KKH) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
SaKKH-ABE8e-dimer	ecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
LbABE8e	ecTadA(8e)-dLbCas12a (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
LbABE8e-dimer	ecTadA(WT)-ecTadA(8e)-dLbCas12a (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
enAsABE8e	ecTadA(8e)-dAsCas12a (En) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
enAsABE8e-dimer	ecTadA(WT)-ecTadA(8e)-dAsCas12a (En) (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
LbABE8e(TadA-8e V106W)	ecTadA(8e V106W)-dLbCas12a (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
SaABE8e(TadA V106W)	ecTadA(8e V106W)-nSaCas9 (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	A-to-G base editor pCMV with a BR322 origin
pBT44c	inteinC-dSpCas9-UGI (Other)			Liu	Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020)	P2 pCMV with a BR322 origin
H446 EF1a RA-ABE6.3 p2A Puro	ABE RA6.3 (Other)	EF1a	Puromycin	Xue	Adenine base editing in an adult mouse model of tyrosinaemia. Nat Biomed Eng. 2019 Feb 25. pii: 10.1038/s41551-019-0357-8. doi: 10.1038/s41551-019-0357-8.	Lentiviral expression of ABE6.3. lenti
pCMV-Target-ACEmax	Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS (Synthetic)	CMV promoter		Yachie	Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.	Mammalian Target-ACEmax expression vector (pSI-625) pcDNA3.1
pCMV-ACBEmax	Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS (Rattus norvegicus)	CMV promoter		Yachie	Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.	Mammalian ACBEmax expression vector (pSI-632) pcDNA3.1
pCMV-Target-AIDmax	Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS (Synthetic)	CMV promoter		Yachie	Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0.	Mammalian Target-AIDmax expression vector (pSI-626) pcDNA3.1
pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)	human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP (Synthetic)	CAG		Kleinstiver	Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP pRZ33 Tag / Fusion Protein P2A-EGFP (C terminal on insert)
pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)	human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP (Synthetic)	CAG		Kleinstiver	Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP pRZ33 Tag / Fusion Protein P2A-EGFP (C terminal on insert)
pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)	human codon optimized CBE4max xCas9(3.7) with P2A-EGFP (Synthetic)	CAG		Kleinstiver	Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP pRZ33 Tag / Fusion Protein P2A-EGFP (C terminal on insert)
pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)	human codon optimized CBE4max SpCas9-NG with P2A-EGFP (Synthetic)	CAG		Kleinstiver	Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP pRZ33 Tag / Fusion Protein P2A-EGFP (C terminal on insert)
pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)	human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP (Synthetic)	CMV and T7		Kleinstiver	Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.	CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP Tag / Fusion Protein P2A-EGFP (C terminal on insert)
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)	human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP (Synthetic)	CMV and T7		Kleinstiver	Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.	CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP Tag / Fusion Protein P2A-EGFP (C terminal on insert)
pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)	human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP (Synthetic)	CMV and T7		Kleinstiver	Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.	CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP pCMV_ABEmax_P2A_GFP Tag / Fusion Protein P2A-EGFP (C terminal on insert)
pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)	human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP (Synthetic)	CMV and T7		Kleinstiver	Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26.	CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP Tag / Fusion Protein P2A-EGFP (C terminal on insert)
pCAG-BE4max-P2A-EGFP (pRZ33)	Human codon optimized pCAG BE4max with P2A-EGFP (Synthetic)	CAG		Joung	Joung Lab Plasmids (unpublished)	CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP pCAG backbone
SPACE (pRZ1813)	SPACE (Other)	CMV		Joung	A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.	CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
SPACE-ΔUGI (pRZ1836)	SPACE-ΔUGI (Other)	CMV		Joung	A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.	CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP CMV
SPACE-NG (pRZ5150)	SPACE_NG (Other)	CMV		Joung	A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.	CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
SPACE-VRQR (pRZ5137)	SPACE_VRQR (Other)	CMV		Joung	A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y.	CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
SECURE BE4max(R33A) (pBM608)	BE4max(R33A) (Rattus norvegicus)	CMV		Joung	CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.	CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP CMV
CGBE1 (pRZ3885)	eUNG-BE4max(R33A)-ΔUGI (Rattus norvegicus)	CMV		Joung	CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.	CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP CMV
miniCGBE1 (pBM1219)	BE4max(R33A)-ΔUGI (Rattus norvegicus)	CMV		Joung	CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.	CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP CMV
CGBE1-VRQR (pBM1192)	eUNG-BE4max(R33A)_VRQR-ΔUGI (Rattus norvegicus)	CMV		Joung	CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.	CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP CMV
miniCGBE1-VRQR (pBM1173)	BE4max(R33A)_VRQR-ΔUGI (Rattus norvegicus)	CMV		Joung	CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.	CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP CMV
CGBE1-NG (pBM1246)	eUNG-BE4max(R33A)_NG-ΔUGI (Rattus norvegicus)	CMV		Joung	CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.	CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP CMV
miniCGBE1-NG (pBM1182)	BE4max(R33A)_NG-ΔUGI (Rattus norvegicus)	CMV		Joung	CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x.	CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP CMV
p2T-CMV-ABEmax-BlastR	ABE7.10-nSpCas9 (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	A-to-G base editor p2T-CMV-BlastR
p2T-CMV-ABEmaxCP1041-BlastR	ABE7.10-nSpCas9 CP1041 (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	A-to-G base editor p2T-CMV-BlastR
p2T-CMV-BE4max-BlastR	BE4-nSpCas9-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	C-to-T base editor p2T-CMV-BlastR
p2T-CMV-EA-BE4max-NG-BlastR	BE4 H47E+S48A-nSpCas9-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	High purity C-to-T base editor p2T-CMV-BlastR
p2T-CMV-BE4maxCP1028-BlastR	BE4-nSpCas9 CP1028-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	C-to-T base editor p2T-CMV-BlastR
p2T-CMV-AIDmax-BlastR	AID-nSpCas9-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	C-to-T base editor p2T-CMV-BlastR
p2T-CMV-CDAmax-BlastR	CDA-nSpCas9-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	C-to-T base editor p2T-CMV-BlastR
p2T-CMV-evoAmax-BlastR	evoA-nSpCas9-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	C-to-T base editor p2T-CMV-BlastR
p2T-CMV-eA3Amax-BlastR	eA3A-nSpCas9-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	C-to-T base editor p2T-CMV-BlastR
p2T-CMV-eA3A-BE5-NG-BlastR	eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	High purity C-to-T base editor p2T-CMV-BlastR
p2T-CMV-eA3AmaxNG-BlastR	eA3A-nSpCas9 NG-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	C-to-T base editor, NG PAM p2T-CMV-BlastR
p2T-CMV-eA3Amax-T31A-NG-BlastR	eA3A-BE4 T31A-nSpCas9-UGI-UGI (Synthetic)	CMV		Liu	Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037.	C-to-T/G/A base editor p2T-CMV-BlastR
CMV-YE1-BE3-FNLS-CMV-mCherry	YE1-BE3-FNLS (Synthetic)	CMV		Yang	A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Nat Methods. 2020 May 18. pii: 10.1038/s41592-020-0832-x. doi: 10.1038/s41592-020-0832-x.	Express YE1-BE3-FNLS unspecified
G1333 DddAtox-N–dSpCas9	bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS			Liu	A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.	expresses split DddAtox-Cas9 construct in mammalian cells pCMV
G1333 DddAtox-C–dSpCas9	bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS			Liu	A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.	expresses split DddAtox-Cas9 construct in mammalian cells pCMV
G1397 DddAtox-N–dSpCas9	bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS			Liu	A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.	expresses split DddAtox-Cas9 construct in mammalian cells pCMV
G1397 DddAtox-C–dSpCas9	bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS			Liu	A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.	expresses split DddAtox-Cas9 construct in mammalian cells pCMV
G1333 DddAtox-N–SaKKH-Cas9(D10A)	G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS			Liu	A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.	expresses split DddAtox-Cas9 construct in mammalian cells pCMV
G1333 DddAtox-C–SaKKH-Cas9(D10A)	G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS			Liu	A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.	expresses split DddAtox-Cas9 construct in mammalian cells pCMV
G1397 DddAtox-N–SaKKH-Cas9(D10A)	G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS			Liu	A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.	expresses split DddAtox-Cas9 construct in mammalian cells pCMV
G1397 DddAtox-C–SaKKH-Cas9(D10A)	G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS			Liu	A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4.	expresses split DddAtox-Cas9 construct in mammalian cells pCMV
Lenti-117G-hyA3A-BE4max	Lenti-117G- hyA3A-BE4max (Synthetic)			Li	Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain. Nat Cell Biol. 2020 Jun;22(6):740-750. doi: 10.1038/s41556-020-0518-8. Epub 2020 May 11.	Lentiviral expression of hyA3A-BE4max Adapted from lentiCRISPRv1
Lenti-117G-hyeA3A-BE4max	Lenti -117G-hyeA3A-BE4max (Synthetic)			Li	Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain. Nat Cell Biol. 2020 Jun;22(6):740-750. doi: 10.1038/s41556-020-0518-8. Epub 2020 May 11.	Lentiviral expression of hyeA3A-BE4max Adapted from lentiCRISPRv1
AID-BE4max-P2A-GFP	AID-BE4max (Synthetic)			Li	Dual base editor catalyzes both cytosine and adenine base conversions in human cells. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 10.1038/s41587-020-0527-y.	Mammalian expression of AID-BE4max-P2A-GFP pCMV
Lenti ABE7.10-N-AIDmax	Lenti ABE7.10-N-AIDmax (Synthetic)			Li	Dual base editor catalyzes both cytosine and adenine base conversions in human cells. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 10.1038/s41587-020-0527-y.	Lentiviral expression of ABE7.10-N-AIDmax Adapted from lentiCRISPRv1
Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP	Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP (Synthetic)			Li	Dual base editor catalyzes both cytosine and adenine base conversions in human cells. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 10.1038/s41587-020-0527-y.	Lentiviral expression of HBG site 1 sgRNA Adapted from lentiCRISPRv2
pCMV-ABE8eWQ	bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS (Homo sapiens)			Bae	Bae lab plasmids (unpublished)	Expresses ABE8eWQ in mammalian cells pCMV
pCMV-ABE-P48R	bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Homo sapiens)			Bae	Bae lab plasmids (unpublished)	Expresses ABE-P48R in mammalian cells pCMV
pCMV-ABE-P48R-UGI	bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS (Homo sapiens)			Bae	Bae lab plasmids (unpublished)	Expresses ABE-P48R-UGI in mammalian cells pCMV
pCMV-ABE7.10-P2A-GFP	ABE7.10-P2A-GFP (Synthetic)	CMV		Kim	Mammalian expression plasmids ABE7.10 with different promoters (unpublished)	Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells pCMV
EFS-ABE7.10-P2A-GFP	ABE7.10-P2A-GFP (Synthetic)	EFS		Kim	Mammalian expression plasmids ABE7.10 with different promoters (unpublished)	Express ABE7.10-P2A-GFP by EFS promoter in mammalian cells pCMV
hPGK-ABE7.10-P2A-GFP	ABE7.10-P2A-GFP (Synthetic)	hPGK		Kim	Mammalian expression plasmids ABE7.10 with different promoters (unpublished)	Express ABE7.10-P2A-GFP by hPGK promoter in mammalian cells pCMV
pCMV_APOBEC1-UdgX-nCas9	APOBEC1-UdgX-nCas9 (Homo sapiens)			Liu	Liu Lab CGBE base editors (unpublished)	Mammalian CG-to-GC base editing pCMV
pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX	POLD2-APOBEC1-UdgX-nCas9-UdgX (Homo sapiens)			Liu	Liu Lab CGBE base editors (unpublished)	Mammalian CG-to-GC base editing pCMV
pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX	POLD2-APOBEC1-UdgX-NG-nCas9-UdgX (Homo sapiens)			Liu	Liu Lab CGBE base editors (unpublished)	Mammalian CG-to-GC base editing pCMV
pCMV_UdgX-APOBEC1-UdgX-nCas9	UdgX-APOBEC1-UdgX-nCas9 (Homo sapiens)			Liu	Liu Lab CGBE base editors (unpublished)	Mammalian CG-to-GC base editing pCMV
pCMV_UdgX-APOBEC1-UdgX-HF-nCas9	UdgX-APOBEC1-UdgX-HF-nCas9 (Homo sapiens)			Liu	Liu Lab CGBE base editors (unpublished)	Mammalian CG-to-GC base editing pCMV
pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9	UdgX-APOBEC1-UdgX-HF-NG-nCas9 (Homo sapiens)			Liu	Liu Lab CGBE base editors (unpublished)	Mammalian CG-to-GC base editing pCMV
pCMV_UdgX-APOBEC1-UdgX-NG-nCas9	UdgX-APOBEC1-UdgX-NG-nCas9 (Homo sapiens)			Liu	Liu Lab CGBE base editors (unpublished)	Mammalian CG-to-GC base editing pCMV
A3G-BE4.4	A3G-BE4.4 (Synthetic)	CMV		Gao	Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome- and transcriptome-wide off-target effects. Sci Adv. 2020 Jul 15;6(29):eaba1773. doi: 10.1126/sciadv.aba1773. eCollection 2020 Jul.	C to T DNA base editing pCMV

Bacteria

Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication
pET42b-BE3	BE3 (Homo sapiens)	T7		Liu	Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
pET42b-HF-BE3	HF-BE3 (Homo sapiens)			Liu	Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
pET28b-BE3∆UGI	BE3∆UGI (Rattus norvegicus)	T7		Kim	Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nat Biotechnol. 2017 May;35(5):475-480. doi: 10.1038/nbt.3852. Epub 2017 Apr 10.
pScI_dCas9-CDA	dCas9-PmCDA1 (Synthetic)	lambda OR		Kondo	Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
pScI_dCas9-CDA_J23119-sgRNA	dCas9-PmCDA1 (Synthetic), Streptococcus pyogenes sgRNA (Synthetic)	lambda OR, J23119		Kondo	Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
pScI_dCas9-CDA-UL	dCas9-PmCDA1-UGI-LVA (Synthetic)	lambda OR		Kondo	Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
pnCasPA-BEC				Ji	CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species. iScience. 2018 Aug 31;6:222-231. doi: 10.1016/j.isci.2018.07.024. Epub 2018 Aug 1.
pBECKP-km	ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase (Other)			Ji	Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
pBECKP-spe	ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase (Other)			Ji	Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
pET42b-A3A-PBE-ΔUGI	APOBEC3A-nCas9-NLS (Other)	T7 promoter		Gao	Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
pET42b-ABE7.10	ABE7.10 (Synthetic)	T7		Huang	Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.
pBECAb-apr				Ji	A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR. Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 10.1016/j.chembiol.2019.09.003.
pCRISPR-cBEST	streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1 (Other)	ermE*/tipA		Weber	Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST. Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20366-20375. doi: 10.1073/pnas.1913493116. Epub 2019 Sep 23.
pCRISPR-aBEST	Streptomyces codon optimized spCas9n (D10A) (Synthetic), streptomyces codon optimized tadA (Synthetic)	tipA, tipA		Weber	Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST. Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20366-20375. doi: 10.1073/pnas.1913493116. Epub 2019 Sep 23.
pSCBE2	BE2 (Other)		Apramycin for Streptomyces selection	Sun	Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
pSCBE3	BE3 (Other)		Apramycin for Streptomyces selection	Sun	Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
pSCBE3-HF	HF-BE3 (Other)		Apramycin for Streptomyces selection	Sun	Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
pSABEd	ABEd (Other)		Apramycin for Streptomyces selection	Sun	Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
pSABEn	ABEn (Other)		Apramycin for Streptomyces selection	Sun	Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
pET-A3A(N57Q)-BE3	6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS (Synthetic)	T7		Joung	Therapeutic base editing of human hematopoietic stem cells. Nat Med. 2020 Apr;26(4):535-541. doi: 10.1038/s41591-020-0790-y. Epub 2020 Mar 16.

Plant

Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
pMonAID_dCas9-PmCDA_Hyg_ALS	SpCas9 (Other)	2x35S	Hygromycin	Kondo	Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.	Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
pMonAID_nCas9-PmCDA_Hyg_ALS	SpCas9 (Other)	2x35S	Hygromycin	Kondo	Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.	Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
pDicAID_nCas9-PmCDA_NptII_Della	SpCas9 (Other)	PcUbi	Kanamycin	Kondo	Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.	Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella pZD_OsU3gYSA_HolgerCas9_NPTII
pDicAID_nCas9-PmCDA-2A-NptII_ETR	SpCas9 (Other)	PcUbi	Kanamycin	Kondo	Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833.	Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr pZD_OsU3gYSA_HolgerCas9_NPTII
pHEE901			Hygromycin	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR pCambia
pHSE901			Hygromycin	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR pCambia
pBSE901			Neomycin (select with G418)	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance pCambia
pXSE901B			Basta	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance pCambia
pXSE901BG			Basta ; Glyphosate	Chen	CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.	CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance pCambia
pB-dCas9-PBE	NLS-APOBEC1-XTEN-dCas9-UGI-NLS	Ubi	Basta	Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in maize pBUE411
pB-nCas9-PBE	NLS-APOBEC1-XTEN-nCas9-UGI-NLS	Ubi	Basta	Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in maize pBUE411
pdCas9-PBE	NLS-APOBEC1-XTEN-dCas9-UGI-NLS	Ubi		Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
pH-dCas9-PBE	NLS-APOBEC1-XTEN-dCas9-UGI-NLS	Ubi	Hygromycin	Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in rice pHUE411
pH-nCas9-PBE	NLS-APOBEC1-XTEN-nCas9-UGI-NLS	Ubi	Hygromycin	Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in rice pHUE411
pnCas9-PBE	NLS-APOBEC1-XTEN-nCas9-UGI-NLS	Ubi		Gao	Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27.	Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
pJY-RpABE	RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA cassette		Hygromycin	Kim	Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x.	binary vector for ABE expression in Arabidopsis pKI1.1R
pJY-RpABE-FT_INF	RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting FT		Hygromycin	Kim	Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x.	binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis pJY-RpABE
pJY-RpABE-PDS3_IMS	RPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting PDS3		Hygromycin	Kim	Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x.	binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis pJY-RpABE
pH-PABE-7-esgRNA	wtTadA-TadA7.10-nCas9-3*NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in rice pCAMBIA1300
pH-PABE-7-sgRNA	wtTadA-TadA7.10-nCas9-3*NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in rice pCAMBIA1300
PABE-1	NLS-wtTadA-TadA7.10-nCas9-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
PABE-2	wtTadA-TadA7.10-nCas9-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
PABE-3	wtTadA-TadA7.10-NLS-nCas9-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
PABE-4	NLS-nCas9-wtTadA-TadA7.10-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
PABE-5	NLS-nCas9-NLS-wtTadA-TadA7.10-NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
PABE-6	wtTadA-TadA7.10-nCas9-2*NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
PABE-7	wtTadA-TadA7.10-nCas9-3*NLS (Other)			Gao	Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.	Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
pYPQ255	rAPOBEC1-pcoCas9D10A-UGI			Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing pNJB91
pYPQ255-x3.7	rAPOBEC1-pcoCas9D10A_x3.7-UGI			Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
pYPQ256	pcoCas9D10A-PmCDA1-UGI			Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing pNJB91
pYPQ256-x3.7	pcoCas9D10A_x3.7-PmCDA1-UGI			Qi	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
pGEL066			Hygromycin	Zhang	Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011.	For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor pZHY988 Tag / Fusion Protein 3xFlag (C terminal on backbone)
pFH45	nSpCas9-ABE7.10 base changer, human codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.	Level0 nSpCas9-ABE7.10 base changer, human codon optimized pICH41308
pFH55	nSpCas9-PmCDA, Arabidopsis codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.	Level0 nSpCas9-PmCDA, Arabidopsis codon optimized pICH41308
pFH79	A3A-PBE, wheat codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.	Level0 A3A-PBE, wheat codon optimized pICH41308
pFH92	nScCas9-ABE7.10, wheat/human codon optimized (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.	Level0 nScCas9-ABE7.10, wheat/human codon optimized pICH41308
pFH70	ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.	Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt pICH47742
pFH84	ZmUBIp::A3A-PBE (wheat codon optimized):NOSt (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.	Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt pICH47742
pFH93	ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt (Synthetic)			Nekrasov	A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2.	Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt pICH47742
pGEL033				Zhang	Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17.	STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing pZHY988
pGEL035				Zhang	Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17.	STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing pZHY988
PABE-7-NG	wtTadA-TadA7.10-nCas9-NG-3XNLS (Synthetic)			Gao	Gao Lab Plasmids (unpublished)	Targeted A to G in rice protoplasts pJIT163
pYPQ265H	hAID-ziSpyMacCas9(D10A)-UGI			Qi	Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020	Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs pNJB91
pYPQ266B	ziSpyMacCas9(D10A)-PmCDA1-UGI			Qi	Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020	Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs pNJB91
pYPQ262m3	ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)			Qi	Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020	Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs pYPQ165

Yeast

Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL	SpCas9 (Other), PmCDA1 (Other)	pGal1, pGal10	LEU2	Kondo	Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729.	Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells pRS315
pRS315e_pGal-dCas9-PmCDA1	SpCas9 (Other)	pGal1	LEU2	Kondo	Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729.	Expresses dCas9-PmCDA1 in yeast cells pRS315
pRS315e_pGal-nCas9(D10A)-PmCDA1	SpCas9 (Other)	pGal1	LEU2	Kondo	Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729.	Expresses nCas9(D10A)-PmCDA1 in yeast cells pRS315
pJT112_GalL_nCDA1-xBE3	nCDA1-xBE3 (Other)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor nCDA1-xBE3 in yeast cells p415
pJT25_GalL_BE3	BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses BE3 in yeast cells p415
pJT45_GalL_nCDA1-BE3	nCDA1-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses nCDA1-BE3 in yeast cells p415
pJT46_GalL_cCDA1-BE3	cCDA1-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses cCDA1-BE3 in yeast cells p415
pJT78_GalL_nCDA1Δ195-BE3	nCDA1Δ195-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses nCDA1Δ195-BE3 in yeast cells p415
pJT79_GalL_nCDA1Δ192-BE3	nCDA1Δ192-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses nCDA1Δ192-BE3 in yeast cells p415
pJT82_GalL_nCDA1Δ198-BE3	nCDA1Δ198-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses nCDA1Δ198-BE3 in yeast cells p415
pJT84_GalL_nCDA1Δ194-BE3	nCDA1Δ194-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses nCDA1Δ194-BE3 in yeast cells p415
pJT85_GalL_nCDA1Δ193-BE3	nCDA1Δ193-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses nCDA1Δ193-BE3 in yeast cells p415
pJT90_GalL_nCDA1Δ190-BE3	nCDA1Δ190-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses nCDA1Δ190-BE3 in yeast cells p415
pJT92_GalL_nCDA1Δ188-BE3	nCDA1Δ188-BE3 (Other)	GalL	LEU2	Bock	Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.	Expresses nCDA1Δ188-BE3 in yeast cells p415
pJT113_GalL_cCDA1-xBE3	cCDA1-xBE3 (Other)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor cCDA1-xBE3 in yeast cells p415
pJT121_GalL_nCDA1-VQRBE3	nCDA1-VQRBE3 (Other)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor nCDA1-VQRBE3 in yeast cells p415
pJT122_GalL_cCDA1-VQRBE3	cCDA1-VQRBE3 (Other)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor cCDA1-VQRBE3 in yeast cells p415
pJT130_GalL_nCDA1-NGBE3	nCDA1-NGBE3 (Other)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor nCDA1-NGBE3 in yeast cells p415
pJT131_GalL_cCDA1-NGBE3	cCDA1-NGBE3 (Other)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor cCDA1-NGBE3 in yeast cells p415
pJT139_GalL_nCDA1-VRERBE3	nCDA1-VRERBE3 (Other)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor nCDA1-VRERBE3 in yeast cells p415
pJT140_GalL_cCDA1-VRERBE3	cCDA1-VRERBE3 (Other)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor cCDA1-VRERBE3 in yeast cells p415
pJT166_GalL_A3A-BE3	A3A-BE3 (Homo sapiens)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor A3A-BE3 in yeast cells p415
pJT169_GalL_A3AΔ186-BE3	A3AΔ186-BE3 (Homo sapiens)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor A3AΔ186-BE3 in yeast cells p415
pJT170_GalL_A3AΔ182-BE3	A3AΔ182-BE3 (Homo sapiens)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor A3AΔ182-BE3 in yeast cells p415
pJT171_GalL_A3AΔ178-BE3	A3AΔ178-BE3 (Homo sapiens)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor A3AΔ178-BE3 in yeast cells p415
pJT173_GalL_A3A-R128A-BE3	A3A(R128A)-BE3 (Homo sapiens)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor A3A-R128A-BE3 in yeast cells p415
pJT174_GalL_A3A-Y130F-BE3	A3A(Y130F)-BE3 (Homo sapiens)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor A3A-Y130F-BE3 in yeast cells p415
pJT175_GalL_A3A(Y130F)Δ186-BE3	A3A(Y130F)Δ186-BE3 (Homo sapiens)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells p415
pJT176_GalL_eA3A-BE3	A3A(N57G)-BE3 (Homo sapiens)	GalL	LEU2	Bock	Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.	Expressing base editor eA3A-BE3 in yeast cells p415

Zebrafish

	Plasmid	Gene/Insert	Promoter	Selectable Marker	PI	Publication	Hidden Extra Search Info
	pCMV-BE-zCas9	rAPOBEC1-XTEN-zCas9-UGI-NLS (Rattus norvegicus)	CMV		Lin	Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6.	expression of an optimized Cas9 for base-editing in zebrafish CMV
	pCMV-BE-VQRCas9	rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS (Rattus norvegicus)	CMV		Lin	Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6.	expression of an optimized Cas9 for base-editing in zebrafish CMV

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