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CRISPR Plasmids: Base Edit


Two classes of base editors--cytosine base editors (CBEs) and adenine base editors (ABEs)--can be used to generate single base pair edits without double stranded breaks.

Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within a small editing window near the PAM site. Uridine is subsequently converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand.)

Likewise, adenine base editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor.

Both types of base editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by optimizing expression of the fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase inhibitor (UGI) or the bacteriophage Mu- derived Gam protein (Mu- GAM).

While many base editors are designed to work in a very narrow window proximal to the PAM sequence, some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window, and are thus well suited to directed evolution applications. Examples of these base editing systems include targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the Bassik lab, in which Cas9 is fused to activation-induced cytidine deaminase (AID).

CRISPR base editing schematic


Browse, sort, or search the tables below for CRISPR base editing plasmids.
Plasmids are available for expression in mammalian systems, bacteria, plants, yeast, and zebrafish.


Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
pCMV-BE1BE1 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE1 with C-terminal NLS in mammalian cells CMV
pCMV-BE2BE2 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE2 in mammalian cells CMV
pCMV-BE3BE3 (Rattus norvegicus)CMV Liu Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature. 2016 Apr 20. doi: 10.1038/nature17946. Expresses BE3 in mammalian cells CMV
pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)SpCas9 (Other)pCMVNeomycin (select with G418) Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells pcDNA3.1
MO91-dCas9-AID(P182X)dCas9-AID P182X (Synthetic)MSCVPuromycin Chang Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10. Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone. M091
pJL-SaBE3SaBE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses SaBE3 in mammalian cells CMV
pJL-SaKKH-BE3SaKKH-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses SaKKH-BE3 in mammalian cells [Edit] CMV
pBK-VQR-BE3VQR-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses VQR-BE3 in mammalian cells CMV
pBK-EQR-BE3EQR-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses EQR-BE3 in mammalian cells CMV
pBK-VRER-BE3VRER-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses VRER-BE3 in mammalian cells CMV
pBK-YE1-BE3YE1-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YE1-BE3 in mammalian cells CMV
pBK-EE-BE3EE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses EE-BE3 in mammalian cells CMV
pBK-YE2-BE3YEE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YE2-BE3 in mammalian cells CMV
pBK-YEE-BE3YEE-BE3 (Rattus norvegicus)CMV Liu Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions. Nat Biotechnol. 2017 Feb 13. doi: 10.1038/nbt.3803. Expresses YEE-BE3 in mammalian cells CMV
pGH335_MS2-AID*Δ-HygroMS2-AID*Δ and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker Addgene #61426
pGH183_MS2-AIDΔDead-HygroMS2-AIDΔDead and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker Addgene #61426
pGH156_MS2-AID-HygroMS2-AID and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker Addgene #61426
pGH153_MS2-AIDΔ-HygroMS2-AIDΔ and Hygromycin resistanceHygromycin Bassik Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker Addgene #61426
pCMV-HF-BE3HF-BE3 (Homo sapiens)CMV Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790. Expresses HF-BE3 with C-terminal NLS in mammalian cells pCMV
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLSrAPOBEC1-nCas9 (D10A)-UGI-NLS (Rattus norvegicus)T7 Kim Highly efficient RNA-guided base editing in mouse embryos. Nat Biotechnol. 2017 Feb 27. doi: 10.1038/nbt.3816. Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells. pET28a
BE4BE (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE4 in mammalian cells pCMV
AID-BE3activation induced cytidine deaminase (Homo sapiens)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses AID-BE3 in mammalian cells pCMV AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
CDA1-BE3CDA1-BE3 (Synthetic)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses CDA1-BE3 in mammalian cells pCMV
SaBE4SaBE4 (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE4 in mammalian cells pCMV
BE4-GamBE4-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE4-Gam in mammalian cells pCMV
BE3-GamBE3-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses BE3-Gam in mammalian cells pCMV
SaBE4-GamSaBE4-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE4-Gam in mammalian cells pCMV
SaBE3-GamSaBE3-Gam (Rattus norvegicus)CMV Liu Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity. Sci Adv. 2017 Aug 30;3(8):eaao4774. doi: 10.1126/sciadv.aao4774. eCollection 2017 Aug. Expresses SaBE3-Gam in mammalian cells pCMV
pCMV-eBE-S1eBE-S1 (Rattus norvegicus)CMV Chen Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. Expresses eBE-S1 in mammalian cells pCMV
pCMV-eBE-S3eBE-S3 (Rattus norvegicus)CMV Chen Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor. Cell Res. 2017 Aug 29. doi: 10.1038/cr.2017.111. Expresses eBE-S3 in mammalian cells pCMV
pCMV-ABE6.3ABE6.3 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE6.3 in mammalian cells pBR322/CMV
pCMV-ABE7.8ABE7.8 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.8 in mammalian cells pBR322/CMV
pCMV-ABE7.9ABE7.9 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.9 in mammalian cells pBR322/CMV
pCMV-ABE7.10ABE7.10 (Synthetic)CMV Liu Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. expresses ABE7.10 in mammalian cells pBR322/CMV
pCMV-dCpf1-BE0dLbCpf1-BE0 (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE0 in mammalian cells CMV
pCMV-dLbCpf1-BE0ΔiNLSdLbCpf1-BE0ΔiNLS (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells CMV
pCMV-dCpf1-BEdLbCpf1-BE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE in mammalian cells CMV
pCMV-dCpf1-BE-YEdLbCpf1-BE-YE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE-YE in mammalian cells CMV
pCMV-dCpf1-BE-YEEdLbCpf1-BE-YEE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-BE-YEE in mammalian cells CMV
pCMV-dCpf1-eBEdLbCpf1-eBE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-eBE in mammalian cells CMV
pCMV-dCpf1-eBE-YEdLbCpf1-eBE-YE (Rattus norvegicus)CMV Chen Base editing with a Cpf1-cytidine deaminase fusion. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. Expresses dLbCpf1-eBE-YE in mammalian cells CMV
pCMV-ABEABE7.10 (Synthetic)CMV Huang Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7. For expression of ABE7.10 in mammalian cells PBR322
pWT063aTRESG-BE3 (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
pWT065a(TCF-LEF)7-BE3 (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
pWT065c(TCF-LEF)7-BE3-P2A-Luc (Synthetic) Liu Rewritable multi-event analog recording in bacterial and mammalian cells. Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. W2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells pACK129
xCas9(3.7)-BE3xCas9(3.7)-BE3 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-BE3 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.7)-BE4xCas9(3.7)-BE4 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-BE4 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.7)-ABE(7.10)xCas9(3.7)-ABE(7.10) (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.7)-ABE pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.6)-BE3xCas9(3.6)-BE3 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-BE3 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.6)-BE4xCas9(3.6)-BE4 (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-BE4 pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • xCas9(3.6)-ABE(7.10)xCas9(3.6)-ABE(7.10) (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9(3.6)-ABE pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
  • A3Ai-Cas9n-UGI-NLSApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3A
    A3Bi-ctd E255A -Cas9n-UGI-NLSApolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
    A3Ai E72A-Cas9n-UGI-NLSApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (Homo sapiens)CMV Harris A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC-Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Res. 2018 May 9. pii: 4994269. doi: 10.1093/nar/gky332. Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal. BE3 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-BE3-2XBE3-2X (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-2X construct (not codon optimized) pCMV
    pCMV-BE3-FNLSBE3-FNLS (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-FNLS construct (not codon optimized) pCMV
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-BE3-P2A-PuroBE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3 (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE3RA-P2A-PuroBE3RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-2X-P2A-PuroBE3RA-2X (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-FNLS-P2A-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-BE3-PGK-PuroBE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3 (not codon optimized) pLL3.3
    pLenti-BE3RA-PGK-PuroBE3RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized) pLL3.3
    pLenti-FNLS-PGK-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized) pLL3.3
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-TRE3G-BE3-PGK-PuroBE3 (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of BE3 (not codon optimized) pLL3.3
    pLenti-TRE3G-BE3RA-PGK-PuroBE3RA (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized) pLL3.3
    pLenti-TRE3G-FNLS-PGK-PuroBE3RA-FNLS (Synthetic)TRE3GPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized) pLL3.3
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-BE3-P2A-GFP-PGK-PuroBE3 (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE3RA-P2A-GFP-PGK-PuroBE3RA (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-FNLS-P2A-GFP-PGK-PuroBE3RA-FNLS (Synthetic)EF1sPuromycin ; GFP Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-xBE3-P2A-PuroxCas9(3.7)-BE3 (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells Adapted from lentiCRISPRv1
    pLenti-xFNLS-P2A-PuroxFNLS(3.7) (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pLenti-xF2X-P2A-PuroxFNLS-2X(3.7) (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
  • Tag / Fusion Protein
    • 3X FLAG (N terminal on insert)
  • pCMV_BE4maxBE4max (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_AncBE4maxAncBE4max (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_ABEmaxABEmax (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
    pCMV_BE4max_3xHABE4max_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
  • pCMV_AncBE4max_3xHAAncBE4max_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
  • pCMV_ABEmax_3xHAABEmax_3xHA (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)
  • pCMV_BE4max_P2A_GFPBE4max_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_AncBE4max_P2A_GFPAncBE4max_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. C:G-to-T:A base editing pCMV
    pCMV_ABEmax_P2A_GFPABEmax_P2A_GFP (Homo sapiens)CMV Liu Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. A:T-to-G:C base editing pCMV
    pLL1 sgRNA(MS2)-MS2-AIDmonoAID mono mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AIDmono pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL2 sgRNA(MS2)-MS2-AID3cAID mono 3c mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AID3c pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL3 sgRNA(MS2)-MS2-AID3fAIDmono 3f mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-AID3f pX330 AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pLL4 sgRNA(MS2)-MS2-hA3ahuman Apobec3a (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pLL5 sgRNA(MS2)-MS2-hA3a D131Ahuman Apobec3a D131A mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a D131A pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pLL6 sgRNA(MS2)-MS2-hA3a D131Ehuman Apobec3a D131E mutant (Homo sapiens)hU6,CBh Meng Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. empty sgRNA cloning vector with MS2-humanA3a D131E pX330 APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-BE3-FNLS(RA)BE3-FNLS (Synthetic)CMV Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. CMV expression vector for BE3-FNLS construct (codon optimized) pCMV
    pLenti-BE4RA-P2A-PuroBE4RA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-BE4GamRA-P2A-PuroBE4GamRA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pLenti-ABE7.10-P2A-PuroABE7.10 (Synthetic)EF1sPuromycin Dow Dow Lab Unpublished Base Editors (unpublished) Lentivirus for constitutive expression of ABE7.10 (not codon optimized) Adapted from lentiCRISPRv1
    pLenti-ABERA-P2A-PuroABERA (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of ABE7.10 RA (codon optimized) Adapted from lentiCRISPRv1
    pLenti-xBE4Gam-P2A-PuroxCas9(3.7)-BE4Gam (Synthetic)EF1sPuromycin Dow Optimized base editors enable efficient editing in cells, organoids and mice. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized) Adapted from lentiCRISPRv1
    pAAV-ABE-NT-sgRNAABE-NT-sgRNA (Other) Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA pAAV
    rat APOBEC1-EGFPapolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Rattus norvegicus)CMV promoterNeomycin (select with G418) Conticello Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells. RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033. plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein pEGFP-N1 Apobec1 REPR, apobec-1
    pEGFP mCherry-ApoB-EGFPmCherry-apoB-EGFP (Synthetic)CMV promoterNeomycin (select with G418) Conticello Flow-cytometric visualization of C>U mRNA editing reveals the dynamics of the process in live cells. RNA Biol. 2015;12(4):389-97. doi: 10.1080/15476286.2015.1026033. Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP pEGFP-N1
    pAAV-ABE-CTABE-CT Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. AAV inverted terminal repeat based vector plasmid encoding the C-terminal half of nCas9 pAAV
    pST1374-scFv-APOBEC-UGI-GB1scFv-rAPOBEC1-UGI-GB1 (Rattus norvegicus)CMV Huang BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4. Expresses scFv-rAPOBEC1-UGI in mammalian cells pST1374
    p3s-ABE7.10ABE 7.10 (Other)CMV Kim Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy. Nat Biotechnol. 2018 Jul;36(6):536-539. doi: 10.1038/nbt.4148. Epub 2018 Apr 27. expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized pCDNA3.1
    pCMV-hA3A-BE3hA3A-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3 in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3B-BE3hA3B-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3B-BE3 in mammalian cells CMV APOBEC3B A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2
    pCMV-hA3C-BE3hA3C-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3C-BE3 in mammalian cells CMV APOBEC3C A3C, APOBEC1L, ARDC2, ARDC4, ARP5, PBI, bK150C2.3
    pCMV-hA3D-BE3hA3D-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3D-BE3 in mammalian cells CMV APOBEC3D A3D, APOBEC3DE, APOBEC3E, ARP6
    pCMV-hA3F-BE3hA3F-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3F-BE3 in mammalian cells CMV APOBEC3F A3F, ARP8, BK150C2.4.MRNA, KA6
    pCMV-hA3G-BE3hA3G-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3G-BE3 in mammalian cells CMV APOBEC3G A3G, ARCD, ARP-9, ARP9, CEM-15, CEM15, MDS019, bK150C2.7, dJ494G10.1
    pCMV-hA3H-BE3hA3H-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3H-BE3 in mammalian cells CMV APOBEC3H A3H, ARP-10, ARP10
    pCMV-hAID-BE3hAID-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hAID-BE3 in mammalian cells CMV AICDA AID, ARP2, CDA2, HEL-S-284, HIGM2
    pCMV-hA1-BE3hA1-BE3 (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA1-BE3 in mammalian cells CMV APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    pCMV-mA3-BE3mA3-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mA3-BE3 in mammalian cells CMV Apobec3 Apobec, Arp3, BC003314, Cem15, Gm20117, Rfv3
    pCMV-mAID-BE3mAID-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mAID-BE3 in mammalian cells CMV Aicda Aid, Arp2
    pCMV-mA1-BE3mA1-BE3 (Mus musculus)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses mA1-BE3 in mammalian cells CMV Apobec1 Cdar1
    pCMV-cAICDA-BE3cAICDA-BE3 (Other)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses cAICDA-BE3 in mammalian cells CMV aicda aid
    pCMV-hA3A-BE3-D131YhA3A-BE3-D131Y (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-D131Y in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-Y132DhA3A-BE3-Y132D (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-Y132D in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-C101ShA3A-BE3-C101S (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-C101S in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-C106ShA3A-BE3-C106S (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-C106S in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCMV-hA3A-BE3-Y130FhA3A-BE3-Y130F (Homo sapiens)CMV Chen Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat Biotechnol., 2018 Expresses hA3A-BE3-Y130F in mammalian cells CMV APOBEC3A A3A, ARP3, PHRBN, bK150C2.1
    pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized AsBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
  • pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized AsBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
  • pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
  • pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
  • pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.3 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
  • pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized enAsBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
  • pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1) (Synthetic) Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized LbBE1.1 pCAG-CFP
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)
  • pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4) (Synthetic)CAG Joung Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. CAG promoter expression plasmid for human codon optimized LbBE1.4 pCAG-CFP
  • Tags / Fusion Proteins
    • 2x SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
  • PB-BE3-NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    PB-BE3-2NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    PB-BE3-3NLSBE3 (Other) Li CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. Stably express BE3 in mammalian cells PiggyBac-mRFP
    A3Bi-Cas9n-UGIAPOBEC3B L1 intron-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction BE3
    A3C-Cas9n-UGIAPOBEC3C-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3C BE3
    A3D-Cas9n-UGIAPOBEC3D-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3D BE3
    A3F-Cas9n-UGIAPOBEC3F-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3F BE3
    A3G-Cas9n-UGIAPOBEC3G-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3G BE3
    A3H HapI-Cas9n-UGIAPOBEC3H Haplotype I-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype I BE3
    A3H HapII-Cas9n-UGIAPOBEC3H Haplotype II-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype II BE3
    A3H HapII R175/6E-Cas9n-UGIAPOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI (Homo sapiens)CMV Harris A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes. Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9. Base editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EE BE3
    CP1012-CBEmaxrApobec1-nSpCas9 (CP1012) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1028-CBEmaxrApobec1-nSpCas9 (CP1028) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1041-CBEmaxrApobec1-nSpCas9 (CP1041) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1249-CBEmaxrApobec1-nSpCas9 (CP1249) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1300-CBEmaxrApobec1-nSpCas9 (CP1300) -UGI-UGI (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. C-to-T base editor pCMV with a BR322 origin
    CP1012-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1012) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1028-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1028) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1041-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1041) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1249-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1249) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    CP1300-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (CP1300) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    VRQR-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (VRQR) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    VRER-ABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (VRER) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    xABEmaxecTadA-ecTadA*(7.10)-nSpCas9 (xCas9) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    SaABEmaxecTadA-ecTadA*(7.10)-nSaCas9 (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    SaKKH-ABEmaxecTadA-ecTadA*(7.10)-nSaCas9 (KKH) (Other) Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV with a BR322 origin
    pSI-Target-AID-NGnSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) Nureki Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129. Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells. pcDNA3.1
    pLV302N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE) (Rattus norvegicus)CMV Schwank Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8. Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells px601
    pLV312.3C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3 (Rattus norvegicus)CMV Schwank Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018 Oct;24(10):1519-1525. doi: 10.1038/s41591-018-0209-1. Epub 2018 Oct 8. Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells px601
    pcDNA3.1(-)-HF2-BE2HF2-BE2 (Synthetic)CMVNeomycin (select with G418) Huang Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5. Expresses HF2-BE2 in mammalian cells pcDNA3.1(-)
    pcDNA3.1(-)-HF2-BE3HF2-BE3 (Synthetic)CMVNeomycin (select with G418) Huang Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein Cell. 2017 Aug;8(8):601-611. doi: 10.1007/s13238-017-0418-2. Epub 2017 Jun 5. Expresses HF2-BE3 in mammalian cells pcDNA3.1(-)
    pcDNA3.1(-)-ABE7.10ABE7.10 (Synthetic)CMVNeomycin (select with G418) Huang Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z. Expresses ABE7.10 in mammalian cells pcDNA3.1(-)
    CAG-FNLS-T2A-EGFP-ires-puroFNLS-T2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance. PyCAG
    CAG-ABEmax-P2A-EGFP-ires-puroABEmax-P2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance. PyCAG
    CAG-xFNLS-T2A-EGFP-ires-puroxFNLS-T2A-EGFP-ires-puro (Other)Puromycin ; EGFP Otonkoski Otonkoski plasmids 2018 (unpublished) CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance. PyCAG
    pBT44c intein-dCas9-ugi fusion (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. BE PACE selection, express C-intein-Cas9-ugi fusion pBT38
    pBT276 CDA1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express CDA1-BE4max in mammalian cells pBT195
    pBT277 evoCDA1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express evoCDA1-BE4max in mammalian cells pBT195
    pBT281 evoAPOBEC1-BE4max (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. express evoAPOBEC1-BE4max in mammalian cells pBT195
    nCas9-UGI-NLS-P2A-EGFP (pJUL1001)nCas9-UGI-NLS-P2A-EGFP (Synthetic)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1). CAG
    BE3-P2A-EGFP (pJUL977)BE3-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP. CAG
    BE3(E63Q)-P2A-EGFP (pRZ189)BE3(E63Q)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant). CAG
    SECURE BE3(R33A)-P2A-EGFP (pJUL1085)BE3(R33A)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A). CAG
    SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)BE3(R33A/K34A)-P2A-EGFP (Rattus norvegicus)CAG Joung Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A). CAG
    NG-ABEmaxTadA-TadA(evo)-Cas9-NG Liu Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 10.1038/s41587-019-0134-y. A-to-G base editor pCMV
    dABEdABE7.10 (Other) Church Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239. Expresses dead Cas9 (dCas9)-ABE7.10 pCMV_ABE7.10
    dCBE4-gamdCBE4-gam (Other) Church Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239. Expresses dead Cas9 (dCas9)-CBE4-gam pCMV
    pBT371CDA1-BE4max-NG (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. Expresses CDA1-BE4max-NG in mammalian cells pBT195
    pBT372evoCDA1-BE4max-NG (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. Expresses evoCDA1-BE4max-NG in mammalian cells pBT195
    pBT375evoAPOBEC1-BE4max-NG (Other) Liu Continuous evolution of base editors with expanded target compatibility and improved activity. Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 10.1038/s41587-019-0193-0. Expresses evoAPOBEC1-BE4max-NG in mammalian cells pBT195
    pCMV-ABEmaxAWABEmax(TadA E59A, TadA* V106W) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. Expresses ABEmaxAW in mammalian cells CMV
    pCMV-ABEmax(TadA E59A)ABEmax(TadA E59A) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. Expresses ABEmax(TadA E59A) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*E59A)ABEmax(TadA E59A TadA* E59A) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*V106M)ABEmax(TadA E59A TadA* V106M) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*V106M) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*V106Q)ABEmax(TadA E59A TadA* V106Q) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*V106Q) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*V106F)ABEmax(TadA E59A TadA* V106F) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*V106F) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*D108W)ABEmax(TadA E59A TadA* D108W) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*D108W) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*D108F)ABEmax(TadA E59A TadA* D108F) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*D108F) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*D108Q)ABEmax(TadA E59A TadA* D108Q) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*D108M)ABEmax(TadA E59A TadA* D108M) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*D108M) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*R47Q)ABEmax(TadA E59A TadA* R47Q) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*R47Q) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*R47F)ABEmax(TadA E59A TadA* D108F) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*R47F) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*R47W)ABEmax(TadA E59A TadA* R47W) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*R47W) in mammalian cells CMV
    pCMV-ABEmax(TadA E59A, TadA*R47M)ABEmax(TadA E59A TadA* R47M) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA E59A, TadA*R47M) in mammalian cells CMV
    pCMV-ABEmax(TadA, TadA*E59A)ABEmax(TadA E59A TadA* E59A) (Homo sapiens) Liu Analysis and minimization of cellular RNA editing by DNA adenine base editors. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. pCMV-ABEmax(TadA, TadA*E59A) in mammalian cells CMV
    pRS0045TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI (Synthetic)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian Target-ACE expression vector pcDNA3.1
    pRS0035nCas9-PmCDA1-UGI (Synthetic)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian Target-AID expression vector pcDNA3.1
    nCas9-UGI control for Target-AID (pRZ1047)NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Other)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1). CAG
    Target-AID (pRZ762)NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP (Other)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP. CAG
    miniABEmax (pRZ900) bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)CMV Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain). CMV
    SECURE miniABEmax-K20A/R21A (pJUL1774) bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)CMV Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations). CMV
    SECURE miniABEmax-V82G (pJUL1828) bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (Other)CMV Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation). CMV
    hA3A-BE3 (pRZ215)hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
    eA3A-BE3 (pRZ206)hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
    hAID-BE3 (pRZ352)hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP (Homo sapiens)CAG Joung CRISPR DNA base editors with reduced RNA off-target and self-editing activities. Nat Biotechnol. 2019 Sep 2. pii: 10.1038/s41587-019-0236-6. doi: 10.1038/s41587-019-0236-6. CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP. CAG
    N-4-BELjCDA1L1_4 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9 Px330
    N-8-BELpCDA1L1_1 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9 Px330
    C-1-BEPmCDA1L1_4 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9 Px330
    C-7-BELpCDA1L1_1 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9 Px330
    C-11-BELpCDA1L2_2 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LpCDA1L2_2 fused to C terminal of nCas9 Px330
    C-12-BELjCDA1L2_1 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9 Px330
    C-13-BELpCDA1L1_4 (Other)U6 with EF-1alpha intron Cheng Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. C-T base editing using LpCDA1L1_4 fused to C terminal of nCas9 Px330
    SauriABEmax Wang A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar. Expresses ABEmax and nSauriCas9 pFS84-CMV-pfs
    SauriBE4max Wang A compact Cas9 ortholog from Staphylococcus Auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biol. 2020 Mar 30;18(3):e3000686. doi: 10.1371/journal.pbio.3000686. eCollection 2020 Mar. Expresses AncBE4max and nSauriCas9 pFS84-CMV-pfs
    epi-ABEmaxBlasticidin Wang Yongming Wang lab plasmids (unpublished) Expresses ABEmax, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
    epi-BE4maxBlasticidin Wang Yongming Wang lab plasmids (unpublished) Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
    epi-ABEmax-NGBlasticidin Wang Yongming Wang lab plasmids (unpublished) Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
    epi-BE4max-NGBlasticidin Wang Yongming Wang lab plasmids (unpublished) Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin epiCRISPR plasmid
    PB-XL-AncBE4maxAncBE4max_GFP (Synthetic)TRE3GBlasticidin Ye Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing. (2019) Nat Commun 10, 5353 Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max XLone-GFP (Addgene plasmid no. 96930)
    ABE8.8-mABE8.8-m Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.8-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.8-dABE8.8-d Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.8-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.13-mABE8.13-m Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.13-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.13-dABE8.13-d Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.13-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.17-mABE8.17-m Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.17-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.17-dABE8.20-d Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.17-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.20-m ABE8.20-m Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.20-m in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    ABE8.20-dABE8.20-dCMV Gaudelli Directed evolution of adenine base editors with increased activity and therapeutic application. Nat Biotechnol. 2020 Apr 13. pii: 10.1038/s41587-020-0491-6. doi: 10.1038/s41587-020-0491-6. expresses ABE8.20-d in mammalian cells pBR322, beta-lactamase maintenance, CMV promoter
    U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHASt1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)CAG, U6, CAG, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHASt1Cas9 LMD9:CNRZ1066 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)CAG, U6, CAG, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHASt1Cas9 LMD9:LMG18311 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)CAG, U6, CAG, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHASt1Cas9 LMD9:TH1477 chimera (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), APOBEC-1 (Homo sapiens), UGI (Homo sapiens), HA tag (Synthetic)CAG, U6, CAG, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625) APOBEC1 APOBEC-1, BEDP, CDAR1, HEPR
    U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9St1Cas9 LMD-9 (Homo sapiens), sgRNA for St1Cas9 (Homo sapiens), ABE7.10 (Homo sapiens)CAG, U6, CAG Doyon Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9. Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub 2020 Jan 3. Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA MSP1594_2x_NLS (Plasmid #110625)
    pLenti-FNLSNG-P2A-PuroFNLSNG (Synthetic)EF1sPuromycin Dow GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pLenti-FNLSHF1-P2A-PuroFNLSHF1 (Synthetic)EF1sPuromycin Dow GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pLenti-FNLSHiFi-P2A-PuroFNLSHiFi (Synthetic)EF1sPuromycin Dow GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pLenti-FNLSHiFiNG-P2A-PuroFNLSHiFiNG (Synthetic)EF1sPuromycin Dow GO: a functional reporter system to identify and enrich base editing activity. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized) Adapted from pLentiCRISPRv1
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pCMV-BE4max-NRRHBE4max-NRRH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses BE4max-NRRH in mammalian cells pBR322 CMV
    pCMV-BE4max-NRTHBE4max-NRTH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses BE4max-NRTH in mammalian cells pBR322 CMV
    pCMV-BE4max-NRCHBE4max-NRCH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses BE4max-NRCH in mammalian cells pBR322 CMV
    pCMV-ABEmax-NRTHABEmax-NRTH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses ABEmax-NRTH in mammalian cells pBR322 CMV
    pCMV-ABEmax-NRCHABEmax-NRCH (Synthetic)CMV Liu Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nat Biotechnology Expresses ABEmax-NRCH in mammalian cells pBR322 CMV
    CMV_Npu-BE3.9max N-terminalNpu-BE3.9max_N-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the N-terminal split-intein fragment of BE3.9max from the CMV promoter pCMV
    CMV_Npu-BE3.9max C-terminalNpu-BE3.9max_C-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the C-terminal split-intein fragment of BE3.9max from the CMV promoter pCMV
    CMV_Npu-ABEmax N-terminalNpu-ABEmax_N-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter pCMV
    CMV_Npu-ABEmax C-terminalNpu-ABEmax_C-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the C-terminal split-intein fragment of ABEmax from the CMV promoter pCMV
    Cbh_v5 AAV-ABE N-terminalv5 AAV-ABE N-terminal (Synthetic)Cbh Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter px601
    Cbh_v5 AAV-ABE C-terminalv5 AAV-CBE C-terminal; U6-protospacer (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA px601
    CMV_Npu-saBE3.9max N-terminalNpu-saBE3.9max_N-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter pCMV
    CMV_Npu-saBE3.9max C-terminalNpu-saBE3.9max_C-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter pCMV
    CMV_Npu-saBE3.9max_KKH C-terminalNpu-saBE3.9max_KKH_C-terminal (Synthetic)CMV Liu Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 10.1038/s41551-019-0501-5. Epub 2020 Jan 14. Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter pCMV
    YE1-BE4maxrAPOBEC1(YE1)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • YE2-BE4maxrAPOBEC1(YE2)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • YEE-BE4maxrAPOBEC1(YEE)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • EE-BE4maxrAPOBEC1(EE)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • YE1-BE4max-NGrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • YE1-BE4max-CP1028rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • AALN-BE4maxrAPOBEC1(AALN)-nSpCas9-UGI-UGI (Other)CMV Liu Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors. Nat Biotechnol. 2020 Feb 10. pii: 10.1038/s41587-020-0414-6. doi: 10.1038/s41587-020-0414-6. C-to-T base editor pBR322/CMV
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
  • pEF-BE4GamBE4Gam (Synthetic)Ef1a Wang A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713. Expression of BE4am-Cas9 Base-editor from an EF1a promoter. pEF
    pEF-AncBE4maxAncBE4max (Synthetic)Ef1a Wang A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713. Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter. pEF
    pCMV-BE4-PpAPOBEC1 H122ABE4-PpAPOBEC1 H122A (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-PpABOBEC1H122A pCMV
    pCMV-BE4-RrA3FBE4-RrA3F (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-RrA3F pCMV
    pCMV-BE4-RrA3F F130LBE4-RrA3F F130L (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-RrA3F F130L pCMV
    pCMV-BE4-AmAPOBEC1BE4-AmAPOBEC1 (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-AmABOBEC1 pCMV
    pCMV-BE4-SsAPOBEC3BBE4-SsAPOBEC3B (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-SsAPOBEC3B pCMV
    pCMV-BE4-SsAPOBEC3B R54QBE4-SsAPOBEC3B R54Q (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q pCMV
    pCMV-BE4-PpAPOBEC1 R33ABE4-PpAPOBEC1 R33A (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-PpABOBEC1 R33A pCMV
    pCMV-BE4-PpAPOBEC1BE4-PpAPOBEC1 (Synthetic)CMV Gaudelli Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity Nat Commun 11, 2052 (2020) Mammalian expression plasmid for BE4-PpABOBEC1 pCMV
    ABE8eecTadA(8e)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    ABE8e-dimerecTadA(WT)-ecTadA(8e)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    NG-ABE8eecTadA(8e)-nSpCas9 (NG) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    CP1028-ABE8eecTadA(8e)-nSpCas9 (CP1028) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    CP1041-ABE8eecTadA(8e)-nSpCas9 (CP1041) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    ABE8e(TadA-8e V82G)ecTadA(8e V82G)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    ABE8e(TadA-8e V106W)ecTadA(8e V106W)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    ABE8e(TadA-8e K20A R21A)ecTadA(8e K20A R21A)-nSpCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    NG-ABE8-dimerecTadA(WT)-ecTadA(8e)-nSpCas9 (NG) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    CP1028-ABE8e-dimerecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    CP1041-ABE8e-dimerecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaABE8eecTadA(8e)-nSaCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaABE8e-dimerecTadA(WT)-ecTadA(8e)-nSaCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaKKH-ABE8eecTadA(8e)-nSaCas9 (KKH) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaKKH-ABE8e-dimerecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    LbABE8eecTadA(8e)-dLbCas12a (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    LbABE8e-dimerecTadA(WT)-ecTadA(8e)-dLbCas12a (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    enAsABE8eecTadA(8e)-dAsCas12a (En) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    enAsABE8e-dimerecTadA(WT)-ecTadA(8e)-dAsCas12a (En) (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    LbABE8e(TadA-8e V106W)ecTadA(8e V106W)-dLbCas12a (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    SaABE8e(TadA V106W)ecTadA(8e V106W)-nSaCas9 (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) A-to-G base editor pCMV with a BR322 origin
    pBT44cinteinC-dSpCas9-UGI (Other) Liu Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity Nat Biotechnol (2020) P2 pCMV with a BR322 origin
    H446 EF1a RA-ABE6.3 p2A PuroABE RA6.3 (Other)EF1aPuromycin Xue Adenine base editing in an adult mouse model of tyrosinaemia. Nat Biomed Eng. 2019 Feb 25. pii: 10.1038/s41551-019-0357-8. doi: 10.1038/s41551-019-0357-8. Lentiviral expression of ABE6.3. lenti
    pCMV-Target-ACEmaxBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS (Synthetic)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian Target-ACEmax expression vector (pSI-625) pcDNA3.1
    pCMV-ACBEmaxBi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS (Rattus norvegicus)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian ACBEmax expression vector (pSI-632) pcDNA3.1
    pCMV-Target-AIDmaxBi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS (Synthetic)CMV promoter Yachie Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. Mammalian Target-AIDmax expression vector (pSI-626) pcDNA3.1
    pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP pRZ33
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP pRZ33
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)human codon optimized CBE4max xCas9(3.7) with P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP pRZ33
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)human codon optimized CBE4max SpCas9-NG with P2A-EGFP (Synthetic)CAG Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP pRZ33
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP pCMV_ABEmax_P2A_GFP
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP (Synthetic)CMV and T7 Kleinstiver Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants Science , 26 Mar 2020 CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP pCMV_ABEmax_P2A_GFP
  • Tag / Fusion Protein
    • P2A-EGFP (C terminal on insert)
  • pCAG-BE4max-P2A-EGFP (pRZ33)Human codon optimized pCAG BE4max with P2A-EGFP (Synthetic)CAG Joung Joung Lab Plasmids (unpublished) CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP pCAG backbone
    SPACE (pRZ1813)SPACE (Other)CMV Joung A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
    SPACE-ΔUGI (pRZ1836)SPACE-ΔUGI (Other)CMV Joung A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP CMV
    SPACE-NG (pRZ5150)SPACE_NG (Other)CMV Joung A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
    SPACE-VRQR (pRZ5137)SPACE_VRQR (Other)CMV Joung A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0535-y. doi: 10.1038/s41587-020-0535-y. CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP CMV
    SECURE BE4max(R33A) (pBM608)BE4max(R33A) (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP CMV
    CGBE1 (pRZ3885)eUNG-BE4max(R33A)-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP CMV
    miniCGBE1 (pBM1219)BE4max(R33A)-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP CMV
    CGBE1-VRQR (pBM1192)eUNG-BE4max(R33A)_VRQR-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP CMV
    miniCGBE1-VRQR (pBM1173)BE4max(R33A)_VRQR-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP CMV
    CGBE1-NG (pBM1246)eUNG-BE4max(R33A)_NG-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP CMV
    miniCGBE1-NG (pBM1182)BE4max(R33A)_NG-ΔUGI (Rattus norvegicus)CMV Joung CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells. Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 10.1038/s41587-020-0609-x. CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP CMV
    p2T-CMV-ABEmax-BlastRABE7.10-nSpCas9 (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. A-to-G base editor p2T-CMV-BlastR
    p2T-CMV-ABEmaxCP1041-BlastRABE7.10-nSpCas9 CP1041 (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. A-to-G base editor p2T-CMV-BlastR
    p2T-CMV-BE4max-BlastRBE4-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-EA-BE4max-NG-BlastRBE4 H47E+S48A-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. High purity C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-BE4maxCP1028-BlastRBE4-nSpCas9 CP1028-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-AIDmax-BlastRAID-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-CDAmax-BlastRCDA-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-evoAmax-BlastRevoA-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-eA3Amax-BlastReA3A-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-eA3A-BE5-NG-BlastReA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. High purity C-to-T base editor p2T-CMV-BlastR
    p2T-CMV-eA3AmaxNG-BlastReA3A-nSpCas9 NG-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T base editor, NG PAM p2T-CMV-BlastR
    p2T-CMV-eA3Amax-T31A-NG-BlastReA3A-BE4 T31A-nSpCas9-UGI-UGI (Synthetic)CMV Liu Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning. Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037. C-to-T/G/A base editor p2T-CMV-BlastR
    CMV-YE1-BE3-FNLS-CMV-mCherryYE1-BE3-FNLS (Synthetic)CMV Yang A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects. Nat Methods. 2020 May 18. pii: 10.1038/s41592-020-0832-x. doi: 10.1038/s41592-020-0832-x. Express YE1-BE3-FNLS unspecified

    Bacteria

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication
    pET42b-BE3BE3 (Homo sapiens)T7 Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
    pET42b-HF-BE3HF-BE3 (Homo sapiens) Liu Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017 Jun 6;8:15790. doi: 10.1038/ncomms15790.
    pET28b-BE3∆UGIBE3∆UGI (Rattus norvegicus)T7 Kim Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Nat Biotechnol. 2017 May;35(5):475-480. doi: 10.1038/nbt.3852. Epub 2017 Apr 10.
    pScI_dCas9-CDAdCas9-PmCDA1 (Synthetic)lambda OR Kondo Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
    pScI_dCas9-CDA_J23119-sgRNAdCas9-PmCDA1 (Synthetic), Streptococcus pyogenes sgRNA (Synthetic)lambda OR, J23119 Kondo Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
    pScI_dCas9-CDA-ULdCas9-PmCDA1-UGI-LVA (Synthetic)lambda OR Kondo Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 10.1038/s41564-017-0102-6.
    pnCasPA-BEC Ji CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species. iScience. 2018 Aug 31;6:222-231. doi: 10.1016/j.isci.2018.07.024. Epub 2018 Aug 1.
    pBECKP-kmncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase (Other) Ji Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
    pBECKP-spencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase (Other) Ji Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase. Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
    pET42b-A3A-PBE-ΔUGIAPOBEC3A-nCas9-NLS (Other)T7 promoter Gao Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
    pET42b-ABE7.10ABE7.10 (Synthetic)T7 Huang Genome-wide profiling of adenine base editor specificity by EndoV-seq. Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.
    pBECAb-apr Ji A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR. Cell Chem Biol. 2019 Sep 17. pii: S2451-9456(19)30277-6. doi: 10.1016/j.chembiol.2019.09.003.
    pCRISPR-cBESTstreptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1 (Other)ermE*/tipA Weber Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST. Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20366-20375. doi: 10.1073/pnas.1913493116. Epub 2019 Sep 23.
    pCRISPR-aBESTStreptomyces codon optimized spCas9n (D10A) (Synthetic), streptomyces codon optimized tadA (Synthetic)tipA, tipA Weber Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST. Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20366-20375. doi: 10.1073/pnas.1913493116. Epub 2019 Sep 23.
    pSCBE2BE2 (Other)Apramycin for Streptomyces selection Sun Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
    pSCBE3BE3 (Other)Apramycin for Streptomyces selection Sun Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
    pSCBE3-HFHF-BE3 (Other)Apramycin for Streptomyces selection Sun Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
    pSABEdABEd (Other)Apramycin for Streptomyces selection Sun Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137
    pSABEnABEn (Other)Apramycin for Streptomyces selection Sun Base editing in Streptomyces with Cas9-deaminase fusions BioRxiv 630137

    Plant

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pMonAID_dCas9-PmCDA_Hyg_ALSSpCas9 (Other)2x35SHygromycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
    pMonAID_nCas9-PmCDA_Hyg_ALSSpCas9 (Other)2x35SHygromycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls pZDgRNA_Cas9ver.2 (D10A)_HPT
    pDicAID_nCas9-PmCDA_NptII_DellaSpCas9 (Other)PcUbiKanamycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella pZD_OsU3gYSA_HolgerCas9_NPTII
    pDicAID_nCas9-PmCDA-2A-NptII_ETRSpCas9 (Other)PcUbiKanamycin Kondo Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion. Nat Biotechnol. 2017 Mar 27. doi: 10.1038/nbt.3833. Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr pZD_OsU3gYSA_HolgerCas9_NPTII
    pHEE901Hygromycin Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR pCambia
    pHSE901Hygromycin Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR pCambia
    pBSE901Neomycin (select with G418) Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance pCambia
    pXSE901BBasta Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance pCambia
    pXSE901BGBasta ; Glyphosate Chen CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance pCambia
    pB-dCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbiBasta Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in maize pBUE411
    pB-nCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbiBasta Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in maize pBUE411
    pdCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbi Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
    pH-dCas9-PBENLS-APOBEC1-XTEN-dCas9-UGI-NLSUbiHygromycin Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411
    pH-nCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbiHygromycin Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in rice pHUE411
    pnCas9-PBENLS-APOBEC1-XTEN-nCas9-UGI-NLSUbi Gao Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 2017 Feb 27. Expresses CRISPR base editor in protoplasts pJIT163-Ubi-GFP
    pJY-RpABERPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA cassetteHygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for ABE expression in Arabidopsis pKI1.1R
    pJY-RpABE-FT_INFRPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting FTHygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis pJY-RpABE
    pJY-RpABE-PDS3_IMSRPS5A promoter (Arabidopsis thaliana), ABE7.10 (Other), U6-sgRNA targeting PDS3Hygromycin Kim Precision genome engineering through adenine base editing in plants. Nat Plants. 2018 Jun 4. pii: 10.1038/s41477-018-0178-x. doi: 10.1038/s41477-018-0178-x. binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis pJY-RpABE
    pH-PABE-7-esgRNAwtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in rice pCAMBIA1300
    pH-PABE-7-sgRNAwtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in rice pCAMBIA1300
    PABE-1NLS-wtTadA-TadA7.10-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-2wtTadA-TadA7.10-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-3wtTadA-TadA7.10-NLS-nCas9-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-4NLS-nCas9-wtTadA-TadA7.10-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-5NLS-nCas9-NLS-wtTadA-TadA7.10-NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-6wtTadA-TadA7.10-nCas9-2*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    PABE-7wtTadA-TadA7.10-nCas9-3*NLS (Other) Gao Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. Targeted A to G in wheat plants or monocotyledons protoplasts pJIT163
    pYPQ255rAPOBEC1-pcoCas9D10A-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing pNJB91
    pYPQ255-x3.7rAPOBEC1-pcoCas9D10A_x3.7-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
    pYPQ256pcoCas9D10A-PmCDA1-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing pNJB91
    pYPQ256-x3.7pcoCas9D10A_x3.7-PmCDA1-UGI Qi Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing pNJB91
    pGEL066Hygromycin Zhang Improving Plant Genome Editing with High-Fidelity xCas9 and Non-canonical PAM-Targeting Cas9-NG. Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 10.1016/j.molp.2019.03.011. For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor pZHY988
  • Tag / Fusion Protein
    • 3xFlag (C terminal on backbone)
  • pFH45nSpCas9-ABE7.10 base changer, human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 nSpCas9-ABE7.10 base changer, human codon optimized pICH41308
    pFH55nSpCas9-PmCDA, Arabidopsis codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 nSpCas9-PmCDA, Arabidopsis codon optimized pICH41308
    pFH79A3A-PBE, wheat codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 A3A-PBE, wheat codon optimized pICH41308
    pFH92nScCas9-ABE7.10, wheat/human codon optimized (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level0 nScCas9-ABE7.10, wheat/human codon optimized pICH41308
    pFH70ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt pICH47742
    pFH84ZmUBIp::A3A-PBE (wheat codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::A3A-PBE (wheat codon optimized):NOSt pICH47742
    pFH93ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt (Synthetic) Nekrasov A modular cloning toolkit for genome editing in plants. BMC Plant Biol. 2020 Apr 23;20(1):179. doi: 10.1186/s12870-020-02388-2. Level1 ZmUBIp::nScCas9-ABE7.10 (wheat/human codon optimized):NOSt pICH47742
    pGEL033 Zhang Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17. STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing pZHY988
    pGEL035 Zhang Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing. Plant Biotechnol J. 2019 Jul;17(7):1431-1445. doi: 10.1111/pbi.13068. Epub 2019 Jan 17. STU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editing pZHY988
    PABE-7-NGwtTadA-TadA7.10-nCas9-NG-3XNLS (Synthetic) Gao Gao Lab Plasmids (unpublished) Targeted A to G in rice protoplasts pJIT163
    pYPQ265HhAID-ziSpyMacCas9(D10A)-UGI Qi Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020 Gateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMs pNJB91
    pYPQ266BziSpyMacCas9(D10A)-PmCDA1-UGI Qi Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020 Gateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMs pNJB91
    pYPQ262m3ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A) Qi Expanding plant genome editing scope with an A-rich PAM targeting iSpyMacCas9 system Plant Communications, 2020 Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs pYPQ165

    Yeast

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHLSpCas9 (Other), PmCDA1 (Other)pGal1, pGal10LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells pRS315
    pRS315e_pGal-dCas9-PmCDA1SpCas9 (Other)pGal1LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses dCas9-PmCDA1 in yeast cells pRS315
    pRS315e_pGal-nCas9(D10A)-PmCDA1SpCas9 (Other)pGal1LEU2 Kondo Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016 Aug 4. pii: aaf8729. Expresses nCas9(D10A)-PmCDA1 in yeast cells pRS315
    pJT112_GalL_nCDA1-xBE3nCDA1-xBE3 (Other)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor nCDA1-xBE3 in yeast cells p415
    pJT25_GalL_BE3BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses BE3 in yeast cells p415
    pJT45_GalL_nCDA1-BE3nCDA1-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses nCDA1-BE3 in yeast cells p415
    pJT46_GalL_cCDA1-BE3cCDA1-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses cCDA1-BE3 in yeast cells p415
    pJT78_GalL_nCDA1Δ195-BE3nCDA1Δ195-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses nCDA1Δ195-BE3 in yeast cells p415
    pJT79_GalL_nCDA1Δ192-BE3nCDA1Δ192-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses nCDA1Δ192-BE3 in yeast cells p415
    pJT82_GalL_nCDA1Δ198-BE3nCDA1Δ198-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses nCDA1Δ198-BE3 in yeast cells p415
    pJT84_GalL_nCDA1Δ194-BE3nCDA1Δ194-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses nCDA1Δ194-BE3 in yeast cells p415
    pJT85_GalL_nCDA1Δ193-BE3nCDA1Δ193-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses nCDA1Δ193-BE3 in yeast cells p415
    pJT90_GalL_nCDA1Δ190-BE3nCDA1Δ190-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses nCDA1Δ190-BE3 in yeast cells p415
    pJT92_GalL_nCDA1Δ188-BE3nCDA1Δ188-BE3 (Other)GalLLEU2 Bock Engineering of high-precision base editors for site-specific single nucleotide replacement. Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8. Expresses nCDA1Δ188-BE3 in yeast cells p415
    pJT113_GalL_cCDA1-xBE3cCDA1-xBE3 (Other)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor cCDA1-xBE3 in yeast cells p415
    pJT121_GalL_nCDA1-VQRBE3nCDA1-VQRBE3 (Other)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor nCDA1-VQRBE3 in yeast cells p415
    pJT122_GalL_cCDA1-VQRBE3cCDA1-VQRBE3 (Other)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor cCDA1-VQRBE3 in yeast cells p415
    pJT130_GalL_nCDA1-NGBE3nCDA1-NGBE3 (Other)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor nCDA1-NGBE3 in yeast cells p415
    pJT131_GalL_cCDA1-NGBE3cCDA1-NGBE3 (Other)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor cCDA1-NGBE3 in yeast cells p415
    pJT139_GalL_nCDA1-VRERBE3nCDA1-VRERBE3 (Other)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor nCDA1-VRERBE3 in yeast cells p415
    pJT140_GalL_cCDA1-VRERBE3cCDA1-VRERBE3 (Other)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor cCDA1-VRERBE3 in yeast cells p415
    pJT166_GalL_A3A-BE3A3A-BE3 (Homo sapiens)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor A3A-BE3 in yeast cells p415
    pJT169_GalL_A3AΔ186-BE3A3AΔ186-BE3 (Homo sapiens)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor A3AΔ186-BE3 in yeast cells p415
    pJT170_GalL_A3AΔ182-BE3A3AΔ182-BE3 (Homo sapiens)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor A3AΔ182-BE3 in yeast cells p415
    pJT171_GalL_A3AΔ178-BE3A3AΔ178-BE3 (Homo sapiens)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor A3AΔ178-BE3 in yeast cells p415
    pJT173_GalL_A3A-R128A-BE3A3A(R128A)-BE3 (Homo sapiens)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor A3A-R128A-BE3 in yeast cells p415
    pJT174_GalL_A3A-Y130F-BE3A3A(Y130F)-BE3 (Homo sapiens)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor A3A-Y130F-BE3 in yeast cells p415
    pJT175_GalL_A3A(Y130F)Δ186-BE3A3A(Y130F)Δ186-BE3 (Homo sapiens)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells p415
    pJT176_GalL_eA3A-BE3A3A(N57G)-BE3 (Homo sapiens)GalLLEU2 Bock Expanding the genome-targeting scope and the site selectivity of high-precision base editors. Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z. Expressing base editor eA3A-BE3 in yeast cells p415

    Zebrafish

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pCMV-BE-zCas9rAPOBEC1-XTEN-zCas9-UGI-NLS (Rattus norvegicus)CMV Lin Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. expression of an optimized Cas9 for base-editing in zebrafish CMV
    pCMV-BE-VQRCas9rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS (Rattus norvegicus)CMV Lin Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. expression of an optimized Cas9 for base-editing in zebrafish CMV


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