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CRISPR Plasmids: Nick


CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR).



Browse, sort, or search the tables below for CRISPR nickase plasmids.
Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.


Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Bacteria

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Drosophila

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Plant

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Yeast

ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication


Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!