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CRISPR Plasmids: Nick


CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead of the double-strand breaks created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close proximity. These double nicks create a double-strand break (DSB) that is repaired using error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR).



Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna Lab.

Browse, sort, or search the tables below for CRISPR nickase plasmids.
Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.


Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Bacteria

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Drosophila

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Plant

Plasmid Gene/Insert Promoter Selectable Marker PI Publication

Yeast

ID Plasmid Description Gene/Insert Promoter Selectable Marker PI Publication


Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!