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Musunuru Lab CRISPR Plasmids Available from Addgene


Human codon-optimized Cas9 nuclease from Streptococcus pyogenes in a plasmid optimized for expression in human pluripotent stem cells and co-expressing enhanced green fluorescent protein (EGFP) for cell selection. The plasmid can be used in any human or mammalian cell.

The Cas9 nuclease is based on the version described in:

RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science . 2013 Feb 15;339(6121):823-6. PubMed .

Resources:

A detailed protocol for the use of these plasmids to perform genome editing of cells is available at StemBook: www.stembook.org/node/1438 . A PDF of the protocol can be downloaded from that page.

This plasmid can be used in combination with the gRNA vector reported in the same publication (Addgene ID 41824 ).

These plasmids are described in:

Enhanced Efficiency of Human Pluripotent Stem Cell Genome Editing through Replacing TALENs with CRISPRs. Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K. Cell Stem Cell . 2013 Apr 4;12(4):393-4. doi: 10.1016/j.stem.2013.03.006. PubMed . PMC .

Individual plasmids can be ordered via the links below:

ID Plasmid
44719 pCas9_GFP : A human codon-optimized Cas9 + EGFP co-expression plasmid. Add to Cart
44720 pCas9D10A_GFP : A human codon-optimized Cas9 nickase + EGFP co-expression plasmid. Add to Cart