CRISPR Plasmids: RNA Editing
Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable RNA editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine, the result is an A->G change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. The T375G mutation reduces off-target effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-984-1090 ADAR2 truncation retain RNA editing capabilities but are small enough to be packaged in AAV particles.
Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna Lab.
Browse, sort, or search the tables below for CRISPR plasmids for RNA editing in mammalian systems.Mammalian
| ID | Plasmid | Gene/Insert | PI | Publication |
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