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CRISPR Plasmids: Activate

Browse CRISPR Plasmids

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CRISPR Resources

Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus.


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Browse, sort, or search the tables below for CRISPR plasmids for transcriptional activation.
Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.

Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
pMSCV-LTR-dCas9-VP64-BFPdCas9-VP64-BFP fusion (Homo sapiens), Puromycin resistanceLTR, PGKPuromycin Qi CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP MSCV-puro
pMSCV-LTR-dCas9-p65AD-BFPdCas9-p65AD-BFP fusion (Homo sapiens), Puromycin resistanceLTR, PGKPuromycin Qi CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP MSCV-puro
pcDNA-dCas9-VP64dCas9-VP64 (Other)CMV Gersbach RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600. Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells pcDNA3.1
Cas9m2-VP64Cas9m2-VP64 (Other) Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Cas9m2 Activator pcDNA3.3_TOPO
Cas9m3-VP64Cas9m3-VP64 (Other) Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Cas9m3 Activator pcDNA3.3_TOPO
Cas9m4-VP64Cas9m4-VP64 (Other) Church CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675. Cas9m4 Activator pcDNA3.3_TOPO
pSL690dCas9-VP64 (Synthetic)CMV Joung CRISPR RNA-guided activation of endogenous human genes. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598. Expresses dCas9-VP64 fusion unknown
pMLM3705codon optimized dCas9-VP64 (Synthetic)CMV Joung CRISPR RNA-guided activation of endogenous human genes. Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598. Expresses mammalian cell codon-optimized dCas9-VP64 pJDS246
pAC1-pCR8-dCas9VP48dCas9(D10A;H840A) fusion with VP48 activation domain (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression pCR8/GW/TOPO
pAC147-pCR8-dCas9VP64dCas9(D10A;H840A) fusion with VP64 activation domain (Homo sapiens) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression  pCR8/GW/TOPO
pAC148-pCR8-dCas9VP96dCas9(D10A;H840A) fusion with VP96 activation domain (Homo sapiens) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression pCR8/GW/TOPO
pAC149-pCR8-dCas9VP160dCas9(D10A;H840A) fusion with VP160 activation domain (Homo sapiens) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression pCR8/GW/TOPO
pAC91-pmax-dCas9VP64dCas9(D10A;H840A) fusion with VP64 activation domain (Homo sapiens)CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP64 on pmax expression vector pmax-DEST (Addgene: 48222)
pAC92-pmax-dCas9VP96dCas9(D10A;H840A) fusion with VP96 activation domain (Homo sapiens)CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP96 on pmax expression vector pmax-DEST (Addgene: 48222)
pAC93-pmax-dCas9VP160dCas9(D10A;H840A) fusion with VP160 activation domain (Homo sapiens)CAGGS Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP160 on pmax expression vector pmax-DEST (Addgene: 48222)
pAC94-pmax-dCas9VP160-2A-purodCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro (Homo sapiens)CAGGSPuromycin Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested. pmax-DEST (Addgene: 48222)
pAC95-pmax-dCas9VP160-2A-neodCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo (Homo sapiens)CAGGSNeomycin (select with G418) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested. pmax-DEST (Addgene: 48222)
pAC2-dual-dCas9VP48-sgExpressiondCas9VP48 (Homo sapiens) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters pX335 (Addgene #42335)
pAC5-dual-dCas9VP48-sgTetOdCas9VP48 and sgTetO (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters pAC2-dual-dCas9VP48-sgExpression (Addgene #48236)
pAC152-dual-dCas9VP64-sgExpressiondCas9 (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters pX335 (Addgene #42335)
  • Tags / Fusion Proteins
    • HA-Tag (N terminal on insert)
    • VP64 (C terminal on insert)
    		•
    pAC153-dual-dCas9VP96-sgExpressiondCas9 (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters pX335 (Addgene #42335)
  • Tags / Fusion Proteins
    • HA-Tag (N terminal on insert)
    • VP96 (C terminal on insert)
    		•
    pAC154-dual-dCas9VP160-sgExpressiondCas9 (Synthetic) Jaenisch Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system. Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122. Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters pX335 (Addgene #42335)
  • Tags / Fusion Proteins
    • HA-Tag (N terminal on insert)
    • VP160 (C terminal on insert)
    		•
    M-SPn-VP64Cas9-VP64, nuclease-null (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized pcDNA3.3 TOPO
    M-ST1n-VP64Cas9-VP64, nuclease-null (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized pcDNA3.3 TOPO
    M-NMn-VP64Cas9-VP64, nuclease-null (Other)CMVNeomycin (select with G418) Church Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized pcDNA3.3 TOPO
    pCMV_dCas9_VP64dCas9_VP64 (human-codon-optimized) (Homo sapiens)CMV Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11. encodes human-optimized dCas9_VP64 synthetic transcription factor phi-Yellow-Dest
    pHAGE TRE dCas9-VP64dCas9 (Other)TRENeomycin (select with G418) Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Tet-regulatable dCas9-VP64 lentiviral expression vector pHAGE
    pHAGE EF1α dCas9-VP64dCas9 (Other)EF1alphaPuromycin Wolfe Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Constitutive dCas9-VP64 lentiviral expression vector pHAGE
    pLV hUbC-dCas9 VP64-T2A-GFPhumanized dead Cas9 VP64 T2A GFP (Other)hUbC Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP FUGW
    CMVp-dCas9-3xNLS-VP64 (Construct 1)dCas9 (Homo sapiens)CMV/hUBC Lu Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells. Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022. Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector. pFUGw (Addgene id: 25870)
    pLV hUbC-VP64 dCas9 VP64-T2A-GFPhumanized VP64 dead Cas9 VP64 T2A GFP (Other) Gersbach Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014 Aug 13. pii: gku749. Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP FUGW
    pcDNA3.1-CibN-dCas9CibN-dCas9 (Arabidopsis thaliana)CMVNeomycin (select with G418) Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses CibN-dCas9 in mammalian cells pcDNA3.1
    pcDNA3.1-dCas9-CibNdCas9-CibN (Arabidopsis thaliana)CMVNeomycin (select with G418) Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses dCas9-CibN in mammalian cells pcDNA3.1
    pcDNA3.1-CibN-dCas9-CibNdCas9-CibN (Arabidopsis thaliana)CMVNeomycin (select with G418) Gersbach A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 2015 Feb 9. doi: 10.1038/nchembio.1753. Expresses CibN-dCas9-CibN in mammalian cells pcDNA3.1
    pHRdSV40-dCas9-10xGCN4_v4-P2A-BFPCas9 dead Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation pHR
    pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLSscFv-GCN4 Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64 pHR
    pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPREdCas9dSV40 Promoter Vale A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging. Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system pHR
    pcDNA-dCas9-FLp300S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (Homo sapiens)CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300
    pcDNA-dCas9-p300 CoreS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300
    pcDNA-dCas9-p300 Core (D1399Y)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300
    pcDNA-dCas9-p300 Core (1645/1646 RR/EE)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300
    pcDNA-dCas9-p300 Core (C1204R)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300
    pcDNA-dCas9-p300 Core (Y1467F)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300
    pcDNA-dCas9-p300 Core (1396/1397 SY/WW)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300
    pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens)CMV Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter pcDNA3.1 EP300 KAT3B, RSTS2, p300
    pcDNA3.3-Nm-dCas9-p300 CoreNm-dCas9-p300 Core (Homo sapiens)CMVNeomycin (select with G418) Gersbach Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. encodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter pcDNA3.3 EP300 KAT3B, RSTS2, p300
    dCAS9-VP64_GFPdCAS9(D10A,H840A)-VP64_2A_GFP (Synthetic)EF1AGFP Zhang Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. Expresses dCAS9-VP64 activator with 2A GFP lenti(AMP)
    lenti dCAS-VP64_BlastdCAS9(D10A, N863A)-VP64_2A_Blast (Synthetic)EF1ABlasticidin Zhang Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) plenti
    TetO-FUW-VdC9BVVP64dCas9BFPVP64 (Synthetic) Leong A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification. Stem Cell Reports. 2014 Dec 9;3(6):940-7. doi: 10.1016/j.stemcr.2014.09.013. Epub 2014 Oct 23. Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes TetO-FUW
    pJZC32sgRNA, MCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC25sgRNA + 1x MS2 binding module, MCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC33sgRNA + 2x MS2 binding module, MCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC34sgRNA + 2x MS2(wt+f6) binding module, MCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC41sgRNA, PCP-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC39sgRNA + 1x PP7, mCherryU6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x PP7 with mCherry for mammalian cells MP177_U6 (derived from pSico)
    pJZC101sgRNA, COM-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC48sgRNA + 1x COM binding module, COM-VP64U6, CMVMarked by mCherry Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x COM with COM-VP64 effector for mammalian cells MP177_U6 (derived from pSico)
    pJZC116sgRNA + 2x MS2 (wt+f6) binding module, MCP-VP64U6, CMVMarked by BFP Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP MP177_U6 (derived from pSico)
    PX855SpCas9 (aa 2-535)CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES) PX330
    PX856SpCas9 (aa536-1368)CBh Zhang A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64) PX330
    SP-dCas9-VPRSP-dCas9-VPR (Homo sapiens)CMVNeomycin (select with G418) Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector pcDNA3.3 TOPO
    M_ST1n_VPRST1-dCas9-VPR (Homo sapiens)CMVNeomycin (select with G418) Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector pcDNA3.3 TOPO
    PB-TRE-dCas9-VPRdCas9-VPR (Homo sapiens)TREHygromycin Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. SP-dCas9-VPR with doxycycline-inducible expression PB-TRE
    NLS-dCas9-trCIB1dCas9-trCIB1 fusion (Synthetic)CMVNeomycin (select with G418) Sato CRISPR-Cas9-based Photoactivatable Transcription System. Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22. Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1 pcDNA3.1/V5-His A
    pJZC42sgRNA + 1XPP7, PCP-VP64 IRES mCherryU6, CMVmCherry fluorescence Lim Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry pSico
    pJZC43sgRNA + 2XPP7, PCP-VP64 IRES mCherryU6, CMVmCherry fluorescence Lim Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry pSico
    pEF_dCas9-VP64dCas9 (Other)human EF1[alpha] Rinn Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1. Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter. pNEB193
  • Tags / Fusion Proteins
    • 3xFLAG (C terminal on insert)
    • VP64 (C terminal on insert)
    		•
    AAV_NLS-dSaCas9-NLS-VPRdSaCas9-VPR (Synthetic)CMV Church Cas9 gRNA engineering for genome editing, activation and repression. Nat Methods. 2015 Sep 7. doi: 10.1038/nmeth.3580. AAV vector containing nuclease null SaCas9 fused to VPR pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA Plasmid #61592
    CAG-DDdCas9VP192-T2A-EGFP-ires-puroDD-dCas9VP192-T2A-EGFP (Other)CAGPuromycin Otonkoski Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim. PyCAG
    pCXLE-dCas9VP192-T2A-EGFP-shP53dCas9-dCas9VP192-GFP-shp53 (Other)CAG Otonkoski Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. Episomal plasmid encoding dCas9VP192 and p53 shRNA pCXLE
    pCXLE-dCas9VP192-T2A-EGFPdCas9-dCas9VP192-EGFP (Other)CAG Otonkoski Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. Episomal plasmid encoding dCas9VP192 pCXLE
    pHRm-NLS-dCas9-GFP11x7-NLS-P2A-BFP-NLSNLS-dCas9-GFP11x7-NLS-P2A-BFP-NLS (Synthetic) Huang Versatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. Expresses NLS-dCas9-GFP11x7-NLS and BFP-NLS in mammalian cells pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE (addgene #60910)
    pAC164-pmax-dCas9Master-VP64dCas9-VP64 (Synthetic)CAGGS + chim intron Cheng Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. dCas9-3xGGGGS-VP64 in pmax expression vector pAC90-pmax-DEST
    pAC1364-pmax-dCas9Master_mCBPHATdCas9-mCBPHAT (Synthetic)CAGGS + chim intron Cheng Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. dCas9Master_mCBPHAT in pmax expression vector pAC90-pmax-DEST
    pAC1410-pmax-dCas9Master_p65HSF1dCas9-p65HSF1 (Synthetic)CAGGS + chim intron Cheng Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 Jan 15. dCas9Master_p65HSF1 in pmax expression vector pAC90-pmax-DEST
    lentiSAMv2U6 and EF1ABlasticidin Zhang Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences. plenti
    pLV-dCas9-p300-P2A-PuroRS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (Homo sapiens), pac from Streptomyces alboniger (Other)EFSPuromycin Gersbach CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome. Nat Biotechnol. 2017 Apr 3. doi: 10.1038/nbt.3853. Lentiviral Sp dCas9-p300-P2A-PuroR lentiCRISPR v2 EP300 KAT3B, RSTS2, p300
    AAVS1-idCas9-vprdCas9-VPR (Other)TRE-tightPuromycin Na An inducible CRISPR-ON system for controllable gene activation in human pluripotent stem cells. Protein Cell. 2017 Jan 23. doi: 10.1007/s13238-016-0360-8. inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus AAVS1 homologous recombineering donor plasmid
    lentiSAM v2 (Puro)U6 (sgRNA) and EF1a (dCas9-VP64)Puromycin Karpf Lenti CRISPR Activate (unpublished) Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences. lentiSAM v2
    pXPR_120dCas9 (Other)EF1aBlasticidin Root Najm et al. (unpublished) for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR pXPR
  • Tag / Fusion Protein
    • VPR (C terminal on insert)
    		•
    lenti-EF1a-dCas9-VP64-PurodCas9-VP64-T2A-Puro (Synthetic)EF-1aPuromycin, Zeocin Brennand Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27. 3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE) pLenti
    lenti-EF1a-dCas9-VPR-Puro(Sp)dCas9-VPR-P2A-Puro (Homo sapiens)EF-1aPuromycin, Zeocin Brennand Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27. 3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE) pLenti
    PB-UniSAMUniSAM-mCherry + U6-gRNA2.0EF1amCherry Forrester An all-in-one UniSam vector system for efficient gene activation Scientific Reports Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0 Piggy Bac
    pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1TMt-dCas9(C)-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether pX856
    pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1VEGFR1-dCas9(C) Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1 pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1BDKRB2-dCas9(N) Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2 pTMt_TCS(Q’G)_NLSHA- dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64BDKRB2-dCas9(C)_DHFR-PCP-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2 pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1AVPR2-dCas9(C)-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to AVPR2 pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1CXCR4-dCas9(C)-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to CXCR4 pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1LPAR1-dCas9(C)-VP64 Fulga Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. Encodes dCas9(C)-VP64 with SAM components fused to LPAR1 pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
    IGI-P0492 pHR-dCas9-NLS-VPR-mCherrydCas9-VPR-mCherry (Homo sapiens)CAGmCherry Corn Corn Lab Cas9 plasmids (unpublished) Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa. pHR
  • Tag / Fusion Protein
    • NLS-VPR-mCherry (C terminal on insert)
    		•
    PB-SAMMS2-P65-HSF1_T2A_Hygro (Homo sapiens), dCAS9(D10A, N863A)-VP64_T2A_Blast (Synthetic)EF1A, CAGHygromycin, Blasticidin Liu One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes. Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15. Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. piggybac
    PB-SAM-DestN/AHygromycin, Blasticidin Liu One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes. Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15. Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes. PB-SAM
    pAW90.dCas9-YY1dCas9-YY1Blasticidin Young YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 10.1016/j.cell.2017.11.008. Epub 2017 Dec 7. Expresses dCas9-YY1 dcas9-vp64
    JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65dLbCpf1(D832A)-p65 (Other)CAG Joung Inducible and multiplex gene regulation using CRISPR-Cpf1-based transcription factors. Nat Methods. 2017 Oct 30. doi: 10.1038/nmeth.4483. Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain pCAG-GFP
    dxCas(3.7)-VPRdxCas(3.7)-VPR (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•
    dxCas(3.6)-VPRdxCas(3.6)-VPR (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus pCMV
  • Tag / Fusion Protein
    • NLS (N terminal on insert)
    		•

    Bacteria

    Plasmid Gene/Insert Promoter PI Publication Hidden Extra Search Info
    pWJ66tracrRNA (Other), dcas9-w (Other), CRISPR array Marraffini Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9) pACYC184
    pWJ68tracrRNA (Other), w-dcas9 (Other), CRISPR array Marraffini Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 2013 Jun 12. Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9) pACYC184
    pET-dCas9-VP64-6xHisdCas9-VP64 (Other)T7 Liu Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. Expression of dCas9-VP64-6xHis in bacterial cells pET29
    pET-deSpCas9-VP64-6xHisdead/inactive eSpCas9-NLS-3xFLAG-VP64 (Other)T7 Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells pET29
    pET-dSpCas9-HF1-VP64-6xHisdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64 (Other)T7 Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells pET29
    pET-dHeFSpCas9-VP64-6xHisdead/inactive HeFSpCas9-NLS-3xFLAG-VP64 (Other)T7 Welker Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells pET29

    Drosophila

    Plasmid Gene/Insert Promoter PI Publication
    pAWG-dCas9-VPRSP-dCas9-VPR (Drosophila melanogaster)act5c Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
    pWalium20-10XUAS-3XFLAG-dCas9-VPRdCas9-VPR (Homo sapiens)10XUAS Perrimon In Vivo Transcriptional Activation Using CRISPR/Cas9 in Drosophila. Genetics. 2015 Oct;201(2):433-42. doi: 10.1534/genetics.115.181065. Epub 2015 Aug 5.
    pAct:dCas9-VPRdCas9-VPRpActin (Drosophila) Perrimon Comparison of Cas9 activators in multiple species. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
    pAct:dCas9-GCN4dCas9-10XGCN4pActin (Drosophila) Perrimon Comparison of Cas9 activators in multiple species. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
    pAct:dCas9-VP64dCas9-VP64pActin (Drosophila) Perrimon Comparison of Cas9 activators in multiple species. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
    pAct:dCas9dCas9pActin (Drosophila) Perrimon Comparison of Cas9 activators in multiple species. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.

    Plant

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pBUN6A11dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic)Ubi1p, OsU3pBar Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector
    pHSN6A01dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic)2×35Sp, AtU6-26pHygromycin Chen A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector
    pD10AH840AhCas9 (GB1041)Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised) (Other) Orzaez GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Plant Physiol. 2013 Jul;162(3):1618-31. Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part pUPD
    pYPQ152pco-dCas9-VP64 (Plant codon-optimized) (Other) Qi A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation. Plant Physiol. 2015 Aug 21. pii: pp.00636.2015. Gateway entry vector with pco-dCas9-VP64 pYPQ185-linker1
    pdCas9 (GB1079)Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised) (Other) Orzaez A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions pUPD

    Yeast

    Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
    pTPGI_dCas9_VP64dCas9_VP64 (codon-optimized for expression in S. cerevisiae) (Saccharomyces cerevisiae)pTPGI (galactose+aTc inducible)TRP1 Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11. encodes yeast-optimized dCas9_VP64 synthetic transcription factor pTPGI (pRS304)
    pJZC519dCas9-VP64 (Other)pTdh3LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Yeast dCas9-VP64 expression plasmid pNH605
    pJZC620MCP-VP64, PCP-VP64, dCas9pAdh, pAdh, pTdh3LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells pNH605
    pJZC638MCP-VP64, dCas9 (Other)pAdh, pGal10LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses MCP-VP64 and dCas9 in Yeast cells pNH605
    pJZC545sgRNA + 1x MS2 binding moduleSNR52URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x MS2 for yeast cells pRS416
    pJZC583sgRNA + 2x MS2 binding moduleSNR52URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 2x MS2 for yeast cells pRS416
    pJZC548sgRNA + 1x PP7SNR52URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x PP7 for yeast cells pRS416
    pJZC603sgRNA + 2x PP7 RNA binding moduleSNR52URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 2x PP7 for yeast cells pRS416
    pJZC572sgRNA + 1x com RNA binding moduleSNR52URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x com for yeast cells pRS416
    pJZC593sgRNA + MS2-PP7 RNA binding moduleSNR52URA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with MS2-PP7 for yeast cells pRS416
    pJZC625sgRNA +1x MS2, Pol II promoter with ribozyme cleavagepAdhURA3 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells pSV616
    pJZC595MCP-VP64, dCas9pAdh, Tdh3LEU2 Qi Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses MCP-VP64 and dCas9 in Yeast cells pNH605
    pAG414GPD-dCas9-VPRdCas9-VPR (Saccharomyces cerevisiae)GPDTRP1 Church Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector pAG414GPD
    pTPGI_dCas9Yeast-optimized dCas9 (Saccharomyces cerevisiae)pTPGITRP1 Lu Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth Biol. 2013 Sep 11. encodes yeast-optimized dCas9 synthetic transcription factor pRS304
    pRS416-Gal4-dCas9-VP64Gal4-dCas9-VP64 (Saccharomyces cerevisiae)Tef1URA3 Davis Dissecting the Genetic Basis of a Complex cis-Regulatory Adaptation. PLoS Genet. 2015 Dec 29;11(12):e1005751. doi: 10.1371/journal.pgen.1005751. eCollection 2015 Dec. This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416. pRS416
    dCas9v2dCas9-VPR (Saccharomyces cerevisiae)URA3 Nielsen Multiplexed CRISPR/Cas9 Genome Editing and Gene Regulation using Csy4 in Saccharomyces cerevisiae. ACS Synth Biol. 2017 Nov 21. doi: 10.1021/acssynbio.7b00259. TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site pDTU-113


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