CRISPR Plasmids: Activate
Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator to your specific locus.
Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna Lab.
Browse, sort, or search the tables below for CRISPR plasmids for transcriptional activation.
Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants, and yeast.
Mammalian
|
|
ID |
Plasmid |
Gene/Insert |
Promoter |
Selectable Marker |
PI |
Publication |
Bacteria
|
|
ID |
Plasmid |
Gene/Insert |
Promoter |
PI |
Publication |
Drosophila
|
|
ID |
Plasmid |
Gene/Insert |
Promoter |
PI |
Publication |
Plant
|
|
ID |
Plasmid |
Gene/Insert |
Promoter |
Selectable Marker |
PI |
Publication |
C. elegans
|
|
ID |
Plasmid |
Gene/Insert |
Promoter |
PI |
Publication |
Yeast
|
|
ID |
Plasmid |
Gene/Insert |
Promoter |
Selectable Marker |
PI |
Publication |
