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CRISPR header icon CRISPR/Cas Plasmids: DNA Purification


A catalytically inactive Cas9 (dCas9) fused to an epitope tag(s) can be used to purify genomic DNA bound by the gRNA. Design your gRNA sequence to direct the dCas9 to a specific genomic sequence. If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9.





Mammalian

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
3xFLAG-dCas9/pCMV-7.13xFLAG-dCas9CMV Fujii Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329-6. doi: 10.1016/j.bbrc.2013.08.013. Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest. pCMV-7.1
3xFLAG-dCas9/pMXs-puro3xFLAG-dCas9 (Synthetic)LTRPuromycin Fujii Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014. Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest. pMXs-puro
3xFLAG-dCas9/pMXs-IG3xFLAG-dCas9 (Synthetic)LTRenhanced GFP Fujii Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014. Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest. pMXs-IG
3xFLAG-dCas9/pMXs-I23xFLAG-dCas9 (Synthetic)LTRhuman CD2 Fujii Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014. Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest. pMXs-I2
3xFLAG-dCas9/pMXs-neo3xFLAG-dCas9 (Synthetic)LTRNeomycin (select with G418) Fujii Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. PLoS One. 2014 Jul 22;9(7):e103084. doi: 10.1371/journal.pone.0103084. eCollection 2014. Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest. pMXs-neo
3xFLAG-dCas9/MSCV-EGFP3xFLAG-dCas9 (Synthetic)LTREGFP Fujii Fujii lab 3xFLAG-dCas9 plasmids (unpublished) Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest. MSCV-EGFP
CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-PurosgRNA and dCas9 from pX330 (Other)U6 for sgRNA and CBh for dCas9Puromycin Kimura Dr. Sekita CRISPR/Cas9 (unpublished) Lentivirus vector to express guideRNA and dCas9 with puro resistant gene CSII
pLenti_dCas9-2xAMSp-dCas9-2xAM tag (Synthetic), gRNA to be inserted into Bbs I sites (Synthetic)CBh, U6Puromycin Fujii Fujii lab enChIP lentiviral plasmids with 2xAM tag (unpublished) Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro
pLenti_dCas9-2xAM_hIRF-1Sp-dCas9-2xAM tag (Synthetic), gRNA targeting human IRF-1 promoter (Synthetic)CBh, U6Puromycin Fujii Fujii lab enChIP lentiviral plasmids with 2xAM tag (unpublished) Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

Yeast

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
3xFLAG-dCas9/pTEF1p-CYC1t3xFLAG-dCas9 (Synthetic)TEF1 promoterTRP1 Fujii Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329-6. doi: 10.1016/j.bbrc.2013.08.013. Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest. p414

Bacteria

Plasmid Gene/Insert Promoter Selectable Marker PI Publication Hidden Extra Search Info
3xFLAG-dCas9/p-bacteria3xFLAG-dCas9 (Synthetic)pLtetO-1 Fujii Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329-6. doi: 10.1016/j.bbrc.2013.08.013. Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest. p15A vector
NM-01Cas9 (Other) Cagney Gerard Cagney unpublished plasmids (unpublished) Bacterial expression of Cas9 with His, MBP, and Strep tags pET21a