|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41824||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||41824-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
Vector backbonepCR-Blunt II-TOPO
Vector typeMammalian Expression, CRISPR
/ Fusion Protein
- AflII site for Gibson Assembly
Growth in Bacteria
- Cloning method TOPO Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
Information for Cloning Grade DNA (Catalog # 41824-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:gRNA_Cloning Vector was a gift from George Church (Addgene plasmid # 41824 ; http://n2t.net/addgene:41824 ; RRID:Addgene_41824)
For your References section:RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722