gRNA_Cloning Vector
(Plasmid #41824)

• Purpose: (Empty Backbone) An empty gRNA expression vector, used to create a gRNA to a specific sequence.
• Depositing Lab: George Church
• Publication: Mali et al Science. 2013 Jan 3.

Backbone

• Vector backbone: pCR-Blunt II-TOPO
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression, CRISPR
• Tag / Fusion Protein:
  • AflII site for Gibson Assembly

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • hCRISPR gRNA Synthesis Protocol
• Articles Citing this Plasmid:
  • 323 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/

Information for Cloning Grade DNA (Catalog # 41824-DNA.cg) ( Back to top )

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here