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RNA-Guided Human Genome Engineering via Cas9.
Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM
Science. 2013 Jan 3.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
41815hCas9Expresses human codon optimized Cas9 nuclease for genome engineering
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41816hCas9_D10AExpresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
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41817gRNA_AAVS1-T1Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering
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41818gRNA_AAVS1-T2Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering
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41819gRNA_GFP-T1Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering
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41820gRNA_GFP-T2Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering
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41821gRNA_DNMT3a-T1Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering
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41822gRNA_DNMT3a-T2Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering
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41823gRNA_DNMT3bExpresses a guide RNA (gRNA) to target DNMT3b for genome engineering
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41824gRNA_Cloning VectorAn empty gRNA expression vector, used to create a gRNA to a specific sequence.
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