PurposeExpresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41819||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCR-Blunt II-TOPO
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
- Cloning method TOPO Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
For more information on Church Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/church/
gRNA target sequence GTGAACCGCATCGAGCTGAA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:gRNA_GFP-T1 was a gift from George Church (Addgene plasmid # 41819 ; http://n2t.net/addgene:41819 ; RRID:Addgene_41819)
For your References section:RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722