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gRNA_GFP-T1 Citations (34)

Originally described in: RNA-Guided Human Genome Engineering via Cas9.
Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM Science. 2013 Jan 3.
PubMed Journal

Articles Citing gRNA_GFP-T1

Targeted genome editing of sweet orange using Cas9/sgRNA. Jia H, Wang N. PLoS One. 2014 Apr 7;9(4):e93806. doi: 10.1371/journal.pone.0093806. eCollection 2014. PubMed
A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice. Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T. BMC Biotechnol. 2014 Jul 21;14:69. doi: 10.1186/1472-6750-14-69. PubMed
The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo. Lin SR, Yang HC, Kuo YT, Liu CJ, Yang TY, Sung KC, Lin YY, Wang HY, Wang CC, Shen YC, Wu FY, Kao JH, Chen DS, Chen PJ. Mol Ther Nucleic Acids. 2014 Aug 19;3:e186. doi: 10.1038/mtna.2014.38. PubMed
Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter. Zhang JH, Pandey M, Kahler JF, Loshakov A, Harris B, Dagur PK, Mo Y, Simonds WF. J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 10.1016/j.jbiotec.2014.08.033. PubMed

Associated Plasmids

Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. Zhou X, Xin J, Fan N, Zou Q, Huang J, Ouyang Z, Zhao Y, Zhao B, Liu Z, Lai S, Yi X, Guo L, Esteban MA, Zeng Y, Yang H, Lai L. Cell Mol Life Sci. 2015 Mar;72(6):1175-84. doi: 10.1007/s00018-014-1744-7. Epub 2014 Oct 2. PubMed
Selection of a hepatitis C virus with altered entry factor requirements reveals a genetic interaction between the E1 glycoprotein and claudins. Hopcraft SE, Evans MJ. Hepatology. 2015 Mar 29. doi: 10.1002/hep.27815. PubMed
Functional disruption of the dystrophin gene in rhesus monkey using CRISPR/Cas9. Chen Y, Zheng Y, Kang Y, Yang W, Niu Y, Guo X, Tu Z, Si C, Wang H, Xing R, Pu X, Yang SH, Li S, Ji W, Li XJ. Hum Mol Genet. 2015 Jul 1;24(13):3764-74. doi: 10.1093/hmg/ddv120. Epub 2015 Apr 9. PubMed
Sequential cancer mutations in cultured human intestinal stem cells. Drost J, van Jaarsveld RH, Ponsioen B, Zimberlin C, van Boxtel R, Buijs A, Sachs N, Overmeer RM, Offerhaus GJ, Begthel H, Korving J, van de Wetering M, Schwank G, Logtenberg M, Cuppen E, Snippert HJ, Medema JP, Kops GJ, Clevers H. Nature. 2015 May 7;521(7550):43-7. doi: 10.1038/nature14415. Epub 2015 Apr 29. PubMed
Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering. Lai S, Wei S, Zhao B, Ouyang Z, Zhang Q, Fan N, Liu Z, Zhao Y, Yan Q, Zhou X, Li L, Xin J, Zeng Y, Lai L, Zou Q. PLoS One. 2016 Jan 12;11(1):e0146562. doi: 10.1371/journal.pone.0146562. eCollection 2016. PubMed
Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. Wei S, Zou Q, Lai S, Zhang Q, Li L, Yan Q, Zhou X, Zhong H, Lai L. Sci Rep. 2016 Jan 19;6:19648. doi: 10.1038/srep19648. PubMed
A non-inheritable maternal Cas9-based multiple-gene editing system in mice. Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T. Sci Rep. 2016 Jan 28;6:20011. doi: 10.1038/srep20011. PubMed
A Library of Infectious Hepatitis C Viruses with Engineered Mutations in the E2 Gene Reveals Growth-Adaptive Mutations That Modulate Interactions with Scavenger Receptor Class B Type I. Zuiani A, Chen K, Schwarz MC, White JP, Luca VC, Fremont DH, Wang D, Evans MJ, Diamond MS. J Virol. 2016 Nov 14;90(23):10499-10512. Print 2016 Dec 1. PubMed
Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques. Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K. Sci Rep. 2016 Oct 26;6:35861. doi: 10.1038/srep35861. PubMed
Generation of Hoxc13 knockout pigs recapitulates human ectodermal dysplasia-9. Han K, Liang L, Li L, Ouyang Z, Zhao B, Wang Q, Liu Z, Zhao Y, Ren X, Jiang F, Lai C, Wang K, Yan S, Huang L, Guo L, Zeng K, Lai L, Fan N. Hum Mol Genet. 2016 Dec 22. pii: ddw378. doi: 10.1093/hmg/ddw378. PubMed
Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids. Fumagalli A, Drost J, Suijkerbuijk SJ, van Boxtel R, de Ligt J, Offerhaus GJ, Begthel H, Beerling E, Tan EH, Sansom OJ, Cuppen E, Clevers H, van Rheenen J. Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):E2357-E2364. doi: 10.1073/pnas.1701219114. Epub 2017 Mar 7. PubMed
Generation of an OCT4 reporter human induced pluripotent stem cell line using CRISPR/SpCas9. Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T. Stem Cell Res. 2017 Aug;23:105-108. doi: 10.1016/j.scr.2017.07.006. Epub 2017 Jul 11. PubMed

Associated Plasmids

Generation of three miR-122 knockout lines from a human embryonic stem cell line. Liu Y, Wu F, Zhuang Y, Guo D, Abbas N, Wei R, Wang K, Chen Y, Tao J, Wu Y, Yuan F, Pan T, Yang F, Lai K, Lai L, Li YX. Stem Cell Res. 2017 Oct;24:164-168. doi: 10.1016/j.scr.2017.06.004. Epub 2017 Jun 9. PubMed
Generation of two MEN1 knockout lines from a human embryonic stem cell line. Liu Y, Ruzi A, Guo D, Wu F, Liu H, Wu H, Wu Y, Yuan F, Zhuang Y, Yang F, Lai K, Lai L, Li YX. Stem Cell Res. 2017 Oct;24:169-173. doi: 10.1016/j.scr.2017.06.005. Epub 2017 Jun 10. PubMed
Generation of a KCNJ11 homozygous knockout human embryonic stem cell line WAe001-A-12 using CRISPR/Cas9. Yuan F, Guo D, Gao G, Liu Y, Xu Y, Wu Y, Yang F, Ke X, Lai K, Hong L, Li YX. Stem Cell Res. 2017 Oct;24:89-93. doi: 10.1016/j.scr.2017.08.016. Epub 2017 Aug 30. PubMed
Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System. Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A. Int J Mol Sci. 2017 Dec 4;18(12). pii: ijms18122610. doi: 10.3390/ijms18122610. PubMed
Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9. Yuan F, Guo D, Liu Y, Xu Y, Gao G, Wu Y, Yang F, Ke X, Lai K, Wei H, Li YX. Stem Cell Res. 2018 Jan;26:67-71. doi: 10.1016/j.scr.2017.11.007. Epub 2017 Nov 10. PubMed
YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Yui S, Azzolin L, Maimets M, Pedersen MT, Fordham RP, Hansen SL, Larsen HL, Guiu J, Alves MRP, Rundsten CF, Johansen JV, Li Y, Madsen CD, Nakamura T, Watanabe M, Nielsen OH, Schweiger PJ, Piccolo S, Jensen KB. Cell Stem Cell. 2018 Jan 4;22(1):35-49.e7. doi: 10.1016/j.stem.2017.11.001. Epub 2017 Dec 14. PubMed
Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing. Wu F, Gao G, Pan T, Yang Z, Xu G, Abbas N, Liu Y, Chen Y, Tan S, You K, Ke X, Zhuang Y, Lin X, Yang F, Li YX. Stem Cell Res. 2018 Mar;27:5-9. doi: 10.1016/j.scr.2017.12.002. Epub 2017 Dec 5. PubMed
Modeling Wnt signaling by CRISPR-Cas9 genome editing recapitulates neoplasia in human Barrett epithelial organoids. Liu X, Cheng Y, Abraham JM, Wang Z, Wang Z, Ke X, Yan R, Shin EJ, Ngamruengphong S, Khashab MA, Zhang G, McNamara G, Ewald AJ, Lin D, Liu Z, Meltzer SJ. Cancer Lett. 2018 Nov 1;436:109-118. doi: 10.1016/j.canlet.2018.08.017. Epub 2018 Aug 23. PubMed
Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing. Wei R, Yuan F, Wu Y, Liu Y, You K, Yang Z, Chen Y, Getachew A, Wang N, Xu Y, Zhuang Y, Yang F, Li YX. Stem Cell Res. 2018 Oct;32:139-144. doi: 10.1016/j.scr.2018.09.010. Epub 2018 Sep 17. PubMed
CRISPR-induced RASGAP deficiencies in colorectal cancer organoids reveal that only loss of NF1 promotes resistance to EGFR inhibition. Post JB, Hami N, Mertens AEE, Elfrink S, Bos JL, Snippert HJG. Oncotarget. 2019 Feb 15;10(14):1440-1457. doi: 10.18632/oncotarget.26677. eCollection 2019 Feb 15. PubMed
Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus. Goldstein JM, Valido A, Lewandowski JP, Walker RG, Mills MJ, Messemer KA, Besseling P, Lee KH, Wattrus SJ, Cho M, Lee RT, Wagers AJ. Sci Rep. 2019 Dec 9;9(1):18613. doi: 10.1038/s41598-019-54766-y. PubMed
NEDD4 and NEDD4L regulate Wnt signalling and intestinal stem cell priming by degrading LGR5 receptor. Novellasdemunt L, Kucharska A, Jamieson C, Prange-Barczynska M, Baulies A, Antas P, van der Vaart J, Gehart H, Maurice MM, Li VS. EMBO J. 2020 Feb 3;39(3):e102771. doi: 10.15252/embj.2019102771. Epub 2019 Dec 23. PubMed
Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9. Sakurai T, Kamiyoshi A, Kawate H, Watanabe S, Sato M, Shindo T. Sci Rep. 2020 Jan 23;10(1):1091. doi: 10.1038/s41598-020-57996-7. PubMed
Plasmodium berghei sporozoites in nonreplicative vacuole are eliminated by a PI3P-mediated autophagy-independent pathway. Bindschedler A, Wacker R, Egli J, Eickel N, Schmuckli-Maurer J, Franke-Fayard BM, Janse CJ, Heussler VT. Cell Microbiol. 2021 Jan;23(1):e13271. doi: 10.1111/cmi.13271. Epub 2020 Oct 8. PubMed
Calcitriol alleviates ethanol-induced hepatotoxicity via AMPK/mTOR-mediated autophagy. Yuan F, Xu Y, You K, Zhang J, Yang F, Li YX. Arch Biochem Biophys. 2021 Jan 15;697:108694. doi: 10.1016/ Epub 2020 Nov 21. PubMed
RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy. Radaszkiewicz T, Noskova M, Gomoryova K, Vondalova Blanarova O, Radaszkiewicz KA, Pickova M, Vichova R, Gybel T, Kaiser K, Demkova L, Kucerova L, Barta T, Potesil D, Zdrahal Z, Soucek K, Bryja V. Elife. 2021 Oct 27;10. pii: 65759. doi: 10.7554/eLife.65759. PubMed
Directed mutational scanning reveals a balance between acidic and hydrophobic residues in strong human activation domains. Staller MV, Ramirez E, Kotha SR, Holehouse AS, Pappu RV, Cohen BA. Cell Syst. 2022 Apr 20;13(4):334-345.e5. doi: 10.1016/j.cels.2022.01.002. Epub 2022 Feb 3. PubMed

Associated Plasmids

Expansion of T memory stem cells with superior anti-tumor immunity by Urolithin A-induced mitophagy. Denk D, Petrocelli V, Conche C, Drachsler M, Ziegler PK, Braun A, Kress A, Nicolas AM, Mohs K, Becker C, Neurath MF, Farin HF, Buchholz CJ, Andreux PA, Rinsch C, Greten FR. Immunity. 2022 Nov 8;55(11):2059-2073.e8. doi: 10.1016/j.immuni.2022.09.014. Epub 2022 Oct 19. PubMed

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