psAAVS1-T2
(Plasmid #60543)

• Purpose: GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities
• Depositing Lab: William Simonds
• Publication: Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 10.1016/j.jbiotec.2014.08.033.

Backbone

• Vector backbone: pEGFP C1
• Backbone manufacturer: ClonTech
• Backbone size w/o insert (bp): 4685
• Total vector size (bp): 4731
• Modifications to backbone: The BsaI site in the backbone was removed by site directed mutagenesis (C3755T).
• Vector type: Mammalian Expression, CRISPR ; Kan

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ProtoSpacer AAVS1-T2
• Alt name: psAAVS1-T2
• Species: H. sapiens (human)
• Insert Size (bp): 46
• Entrez Gene: AAVS1 (a.k.a. AAV)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (unknown if destroyed)
• 3′ cloning site: BsaI (unknown if destroyed)
• 5′ sequencing primer: CCA TTGACGTCAA TGGGAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry