pHAGE-BFP
(Plasmid #106282)

• Purpose: A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES.
• Depositing Lab: Jay Shendure
• Publication: Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.4604. doi: 10.1038/nmeth.4604.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	106282	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

• Vector backbone: pHAGE
  (Search Vector Database)
• Total vector size (bp): 8442
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: BFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
• Species: Synthetic
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • phage_bfp_ires_dsred_wpre_mcs.gb
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry