pLJM1-EGFP-BarcodeV5
(Plasmid #78629)

• Purpose: GESTALT V5 integrating barcode with Puro and EGFP
• Depositing Lab: Jay Shendure
• Publication: McKenna et al Science. 2016 May 26. pii: aaf7907.

Backbone

• Vector backbone: pLJM1-EGFP
• Backbone manufacturer: https://www.addgene.org/19319/
• Backbone size w/o insert (bp): 8083
• Total vector size (bp): 8408
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GESTALT lineage tracing target V5
• Alt name: V5
• Species: H. sapiens (human)
• Insert Size (bp): 323

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGAATTCTCGACCTCGAGACA
• 3′ sequencing primer: CGAAGCTTGAGCTCGAGATCTGAGT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: https://www.addgene.org/19319/

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene 19319 - pLJM1-EGFP, with GESTALT V5 barcode insert at the 3' terminus of EGFP