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Whole organism lineage tracing by combinatorial and cumulative genome editing.
McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
Science. 2016 May 26. pii: aaf7907.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
78627pTol2-DRv7GESTALT V7 barcode for zebrafish
78628pTol2-DRv6GESTALT V6 barcode for zebrafish
78629pLJM1-EGFP-BarcodeV5GESTALT V5 integrating barcode with Puro and EGFP
78630pLJM1-EGFP-BarcodeV4GESTALT V4 integrating barcode with Puro and EGFP
78631pLJM1-EGFP-BarcodeV3GESTALT V3 integrating barcode with Puro and EGFP
78632pLJM1-EGFP-BarcodeV2GESTALT V2 integrating barcode with Puro and EGFP
78633pLJM1-EGFP-BarcodeV1GESTALT V1 integrating barcode with Puro and EGFP
103061GESTALT_pX330-v1plasmid px330 with guide targeting GESTALT v1 through V5 constructs
103062pLenti-Crispr-V2-HMID.v1LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Antibodies from Article