GESTALT_pX330-v1
(Plasmid #103061)

• Purpose: plasmid px330 with guide targeting GESTALT v1 through V5 constructs
• Depositing Lab: Jay Shendure
• Publication: McKenna et al Science. 2016 May 26. pii: aaf7907.

Backbone

• Vector backbone: lentiCRISPR v2 (#52961)
• Modifications to backbone: removal of spacer and insertion of guide sequence
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: integration of Cas9 with guide targeting GESTALT barcodes V1 to V5
• gRNA/shRNA sequence: GGCACTGCGGCTGGAGGTGG
• Species: H. sapiens (human)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (destroyed during cloning)
• 3′ cloning site: BsmBI (destroyed during cloning)
• 5′ sequencing primer: ggactatcatatgcttaccgt
• 3′ sequencing primer: taccgtaagttatgtaacgggtacc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Derived from pX330-U6-Chimeric_BB-CBh-hSpCas9