On the design of CRISPR-based single-cell molecular screens.
Hill AJ, McFaline-Figueroa JL, Starita LM, Gasperini MJ, Matreyek KA, Packer J, Jackson D, Shendure J, Trapnell C
Nat Methods. 2018 Feb 19. pii: nmeth.4604. doi: 10.1038/nmeth.4604.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106280 | CROP-seq-opti | A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al. |
| 106281 | pHAGE-GFP | A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed. |
| 106282 | pHAGE-BFP | A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed. |