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On the design of CRISPR-based single-cell molecular screens.
Hill AJ, McFaline-Figueroa JL, Starita LM, Gasperini MJ, Matreyek KA, Packer J, Jackson D, Shendure J, Trapnell C
Nat Methods. 2018 Feb 19. pii: nmeth.4604. doi: 10.1038/nmeth.4604.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
106280CROP-seq-optiA version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
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106281pHAGE-GFPA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES.
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106282pHAGE-BFPA version of the pHAGE vector with BFP and dsRed expressed separated by an IRES.
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