PurposeCas9n (D10A nickase mutant) from S. pyogenes
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48873||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||48873-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Total vector size (bp) 8500
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Insert Size (bp)5400
MutationCas9 D10A nickase mutant
- Promoter Cbh
/ Fusion Protein
- 3XFLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer TTTATGGCGAGGCGGCGG (Common Sequencing Primers)
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/.
Information for Cloning Grade DNA (Catalog # 48873-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSpCas9n(BB) (PX460) was a gift from Feng Zhang (Addgene plasmid # 48873 ; http://n2t.net/addgene:48873 ; RRID:Addgene_48873)
For your References section:Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. 10.1038/nprot.2013.143 PubMed 24157548