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Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
48137pSpCas9 (PX165)Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)
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48138pSpCas9(BB)-2A-GFP (PX458)Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA
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48139pSpCas9(BB)-2A-Puro (PX459)NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.
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48140pSpCas9n(BB)-2A-GFP (PX461)Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA
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48141pSpCas9n(BB)-2A-Puro (PX462)NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987
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48873pSpCas9n(BB) (PX460)Cas9n (D10A nickase mutant) from S. pyogenes
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62987pSpCas9n(BB)-2A-Puro (PX462) V2.0Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
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62988pSpCas9(BB)-2A-Puro (PX459) V2.0Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
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