Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed December 24 - January 1, 2020. For more information, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Genome engineering using the CRISPR-Cas9 system.
Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
PubMed Article

Plasmids from Article

ID Plasmid  
48137pSpCas9 (PX165)
48138pSpCas9(BB)-2A-GFP (PX458)
48139pSpCas9(BB)-2A-Puro (PX459)
48140pSpCas9n(BB)-2A-GFP (PX461)
48141pSpCas9n(BB)-2A-Puro (PX462)
48873pSpCas9n(BB) (PX460)
62987pSpCas9n(BB)-2A-Puro (PX462) V2.0
62988pSpCas9(BB)-2A-Puro (PX459) V2.0