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Michael Bassik Lab Plasmids


The Michael Bassik Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions. Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC Nat Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
85405pGH020_sgRNA_G418-GFPG418 resistance and GFPMammalian Expression, Lentiviral, CRISPRDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. hu6 driven sgRNA vector with G418 and GFP selectable markers original addgene plasmid #61426 from Feng Zhang lab was modified by replacing p65-HSF1 with AID*Δ Add to Cart
85406pGH335_MS2-AID*Δ-HygroMS2-AID*Δ and Hygromycin resistanceMammalian Expression, Lentiviral, CRISPRDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker Addgene #61426 Add to Cart
85407pGH314_wtGFP S65T, Q80HwtGFP S65T, Q80HMammalian Expression, LentiviralDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for wtGFP S65T-Q80H pSicoR_no puro Add to Cart
85408pGH311_wtGFP S65TwtGFP S65TMammalian Expression, LentiviralDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for wtGFP S65T pSicoR_no puro Add to Cart
85409pGH312_wtGFP Q80HwtGFP Q80HMammalian Expression, LentiviralDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for wtGFP Q80H pSicoR_no puro Add to Cart
85410pGH220_wtGFPwtGFPMammalian Expression, LentiviralDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for wtGFP pSicoR_no puro Add to Cart
85411pGH045_EGFPEGFPMammalian Expression, LentiviralDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for GFP pSicoR_EGFP without PuroR Add to Cart
85412pGH044_mCherrymCherryMammalian Expression, LentiviralDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for mCherry pSicoR_mCherry without PuroR Add to Cart
85413pGH224_sgRNA_2xMS2_Puropuromycin resistanceMammalian Expression, Lentiviral, CRISPRDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker Addgene #61427 Add to Cart
85414pGH183_MS2-AIDΔDead-HygroMS2-AIDΔDead and Hygromycin resistanceMammalian Expression, Lentiviral, CRISPRDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker Addgene #61426 Add to Cart
85415pGH156_MS2-AID-HygroMS2-AID and Hygromycin resistanceMammalian Expression, Lentiviral, CRISPRDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker Addgene #61426 Add to Cart
85416pGH153_MS2-AIDΔ-HygroMS2-AIDΔ and Hygromycin resistanceMammalian Expression, Lentiviral, CRISPRDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker Addgene #61426 Add to Cart
85417pGH125_dCas9-BlastdCas9 and Blasticidin resistanceMammalian Expression, Lentiviral, CRISPRDirected evolution using dCas9-targeted somatic hypermutation in mammalian cells. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. lentiviral expression vector for dCas9 with Blasticidin selectable marker Addgene #61425 Add to Cart
89358pKHH030GFP, Puromycin resistanceMammalian Expression, Lentiviral, CRISPRSynergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions. Nat Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834. hU6 sgRNA cloning vector for CDKO system pMCB306, with addition of hU6 and BbsI sites for cloning of sgRNA Add to Cart
89359pMCB320puromycin resistance, mCherryMammalian Expression, Lentiviral, CRISPRSynergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions. Nat Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834. sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites pMCB306; mCherry is in place of GFP Add to Cart
89360pMCB306GFP, puromycin resistanceMammalian Expression, Lentiviral, CRISPRSynergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions. Nat Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834. sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites pSicoR Add to Cart