Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas.
Farzadfard F, Perli SD, Lu TK
ACS Synth Biol. 2013 Sep 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
49013pTPGI_dCas9_VP64encodes yeast-optimized dCas9_VP64 synthetic transcription factor
Loading...
49014pRPR1_gRNA_handle_RPR1tgRNA expression empty vector (yeast)
Loading...
49015pCMV_dCas9_VP64encodes human-optimized dCas9_VP64 synthetic transcription factor
Loading...
49016pU6_gRNA_handle_U6tgRNA expression empty vector (mammalian cells)
Loading...
62966pRPR1(1xTetO)_gRNA_handle_RPR1taTc inducible gRNA expression vector
Loading...
64279pTPGI_dCas9encodes yeast-optimized dCas9 synthetic transcription factor
Loading...
64378pRPR1_a1gRNA_RPR1tencodes a1 gRNA
Loading...
64379pRPR1_c1gRNA_RPR1tencodes c1 gRNA
Loading...
64380pRPR1_c2gRNA_RPR1tencodes c2 gRNA
Loading...
64381pRPR1_c3gRNA_RPR1tencodes c3 gRNA
Loading...
64382pRPR1_c4gRNA_RPR1tencodes c4 gRNA
Loading...
64383pRPR1_c5gRNA_RPR1tencodes c5 gRNA
Loading...
64384pRPR1_c6gRNA_RPR1tencodes c6 gRNA
Loading...
64385pRPR1_c7gRNA_RPR1tencodes c7 gRNA
Loading...
64386pRPR1_c8gRNA_RPR1tencodes c8 gRNA
Loading...
64387pRPR1_g1gRNA_RPR1tencodes g1 gRNA
Loading...
64388pRPR1_g2gRNA_RPR1tencodes g2 gRNA
Loading...
64389pCYC1m_yeGFPencodes yeast enhanced GFP under control of minimal pCYC1 promoter
Loading...
643901x_a1gRNAOp_pCYC1m_yeBFP2encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP
Loading...
643913x_a1gRNAOp_pCYC1m_yeBFP2encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP
Loading...
643926x_a1gRNAOp_pCYC1m_yeBFP2encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP
Loading...
6439312x_a1gRNAOp_pCYC1m_yeBFP2encodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP
Loading...