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TMPRSS2 g1 + Cas9
(Plasmid #153015)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153015 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Lentiviral Prep 153015-LV Virus (1mL at titer ≥ 5x10⁵ TU/mL) and Plasmid. $180


  • Vector backbone
    Lenti-Cas9-gRNA-TagBFP2 (Addgene #124774)
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    Cas9 + TMPRSS2 gRNA
  • gRNA/shRNA sequence
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • TagBFP2

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Information for Lentiviral Prep (Catalog # 153015-LV) ( Back to top)


Ready-to-use Lentiviral Prep particles produced from TMPRSS2 g1 + Cas9 (#153015). In addition to the viral particles, you will also receive purified TMPRSS2 g1 + Cas9 plasmid DNA.

Lentiviral particles carrying a guide RNA targeting TMPRSS2 and co-expressing Cas9 and TagBFP2.


  • Volume 1mL
  • Titer ≥5x10⁵ TU/mL
  • Pricing $150 USD for preparation of 1mL virus + $30 USD for plasmid.
  • Storage Store at -80℃. Thaw just before use and keep on ice.
  • Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Viral Production & Use

  • Packaging Plasmids psPAX2 (plasmid #12260)
  • Envelope pMD2.G (plasmid #12259)
  • Buffer OptiPro


Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide

Terms and Licenses

Viral Quality Control

Titering Method:
  • ddPCR assay: 293T cells were transduced with serial dilutions of 153015-LV, harvested several days later, and genomic DNA was isolated. Copies of RRE were measured and normalized to RPP30.
  • PCR confirmation of insert: PCRs were carried out on the viral preparation with primers targeting the Cas9 and TagBFP genes to confirm inserts and primers targeting the EF1a promoter and the TMPRSS2 guide to confirm the backbone and guide sequences. The PCR products were visualized on an agarose gel for size confirmation.

Visit our viral production page for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMPRSS2 g1 + Cas9 was a gift from Jason Sheltzer (Addgene plasmid # 153015 ; ; RRID:Addgene_153015) For viral preps, please replace (Addgene plasmid # 153015) in the above sentence with: (Addgene viral prep # 153015-LV)