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Jason Sheltzer Lab: CRISPR plasmids to knock out SARS-CoV-2 host genes
Unpublished

Plasmids from Article

ID Plasmid Purpose  
153011ACE2 g1 + Cas9A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
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153012ACE2 g2A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
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153013ACE2 g3A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
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153014ACE2 g4 + Cas9A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
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153015TMPRSS2 g1 + Cas9A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
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153016TMPRSS2 g2A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
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153017TMPRSS2 g3A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing mCherry
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153018TMPRSS2 g4 + Cas9A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
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