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hACE2
(Plasmid #1786)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1786 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Angiotensin-converting enzyme 2
  • Alt name
    ACE2
  • Alt name
    SARS Receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • GenBank ID
    NM_021804
  • Entrez Gene
    ACE2 (a.k.a. ACEH)
  • Tag / Fusion Protein
    • C9 (GTETSQVAPA) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hACE2 was a gift from Hyeryun Choe (Addgene plasmid # 1786 ; http://n2t.net/addgene:1786 ; RRID:Addgene_1786)
  • For your References section:

    Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, Somasundaran M, Sullivan JL, Luzuriaga K, Greenough TC, Choe H, Farzan M. Nature. 2003 Nov 27. 426(6965):450-4. 10.1038/nature02145 PubMed 14647384