PurposeExpression of TMPRSS2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53887||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
|Cloning Grade DNA||53887-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95 *|
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Backbone manufacturerLawson Lab (Addgene Plasmid 22423)
- Backbone size w/o insert (bp) 4095
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1479
Entrez GeneTMPRSS2 (a.k.a. PRSS10)
- Promoter SP6 and CMV
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EBV-Rev (Common Sequencing Primers)
Information for Cloning Grade DNA (Catalog # 53887-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TMPRSS2 was a gift from Roger Reeves (Addgene plasmid # 53887 ; http://n2t.net/addgene:53887 ; RRID:Addgene_53887)
For your References section:Survey of Human Chromosome 21 Gene Expression Effects on Early Development in Danio rerio. Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, McCallion AS, Katsanis N, Reeves RH. G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 10.1534/g3.118.200144. 10.1534/g3.118.200144 PubMed 29760202