Chemogenetics Plasmid Collection
Background
Chemogenetics plasmids encode genetically engineered receptors that are activated by specific small molecules, allowing a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic receptors are popular in neuroscience, where they can be used to interrogate brain function and examine the relationship between neuronal signaling and behavior. Depending on the signalling pathway the engineered receptor is coupled to and the specific cell type the receptor is expressed in, these receptors can be used to artificially activate or inhibit specific biological processes.
Addgene’s collection contains two main classes of chemogenetic receptors: Designer Receptors Activated by Designer Drugs (DREADDs) and Pharmacologically Selective Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-coupled protein receptors that have been engineered to respond to specific physiologically inert small molecules. There are four types DREADDs in Addgene’s collection: hMD3 (Gq), rMD3 (Gs), hMD4 (Gi) and KORD. hMD3(Gq), rMD3(Gs), and hMD4(Gi) are all based on muscarinic receptors and couple to the Gi, Gq, or Gs G-proteins, respectively. KORD is derived from the κ-opoid receptor and couples to the Gi G-protein. hMD3(Gq) and rMD3(Gs) are activating, while hMD4(Gi) and KORD are inhibitory.
PSAMs are engineered α7 nicotinic acetylcholine receptor (nAChR) domains that respond specifically to small molecules termed Pharmacologically Selective Effector Molecules (PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether a PSAM is activating or inhibitory depends on the ion pore domain it is coupled to. Addgene has two main types of PSAMs: 5HT3 coupled, which are activating, and Gly-coupled, which are inhibitory.
Use the tables below to find the best DREADD or PSAM for your experiment. For a more complete description about chemogenetic receptors, ligands used for chemogenetic studies, and how to use these tools in your experiments, please see our Chemogenetics Guide.
Chemogenetic Plasmids
This table contains Addgene's collection of chemogenetic plasmids plasmids. The table below can be sorted by receptor type, plasmid type, promoter, reporter or fusion protein, and whether the plasmid is Cre- or Flp- dependent. We also offer ready-to-use AAV preparations of select chemogenetic plasmids; these are noted with the viral service icon. 
| ID | Plasmid | Receptor | Promoter | Reporter/Fusion | Cre-dependent? | PI | |
|---|---|---|---|---|---|---|---|
| 50476 | pAAV-CaMKIIa-hM3D(Gq)-mCherry |
hM3D (Gq) | CaMKIIa | mCherry | No | Roth | |
| 50477 | pAAV-CaMKIIa-hM4D(Gi)-mCherry |
hM4D (Gi) | CaMKIIa | mCherry | No | Roth | |
| 65418 | pAAV-CaMKIIa-HA-KORD-IRES-mCitrine | KORD | CaMKIIa | IRES-mCitrine | No | Roth | |
| 44361 | pAAV-hSyn-DIO-hM3D(Gq)-mCherry |
hM3D (Gq) | Syn | mCherry | Yes | Roth | |
| 44362 | pAAV-hSyn-DIO-hM4D(Gi)-mCherry |
hM4D (Gi) | Syn | mCherry | Yes | Roth | |
| 50458 | pAAV-hSyn-DIO-rM3D(Gs)-mCherry | rM3D (Gs) | Syn | mCherry | Yes | Roth | |
| 50474 | pAAV-hSyn-hM3D(Gq)-mCherry |
hM3D (Gq) | Syn | mCherry | No | Roth | |
| 50475 | pAAV-hSyn-hM4D(Gi)-mCherry |
hM4D (Gi) | Syn | mCherry | No | Roth | |
| 65417 | pAAV-hSyn-dF-HA-KORD-IRES-mCitrine |
KORD | Syn | IRES-mCitrine | Yes | Roth | |
| 50478 | pAAV-GFAP-hM3D(Gq)-mCherry |
hM3D (Gq) | GFAP | mCherry | No | Roth | |
| 50479 | pAAV-GFAP-hM4D(Gi)-mCherry |
hM4D (Gi) | GFAP | mCherry | No | Roth | |
| 75033 | pAAV CD68-hM4D(Gi)-mCherry |
hM4D (Gi) | CD68 | mCherry | No | Roth | |
| 50454 | pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine |
hM3D (Gq) | Syn | IRES-mCitrine | Yes | Roth | |
| 50455 | pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine | hM4D (Gi) | Syn | IRES-mCitrine | Yes | Roth | |
| 50456 | pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine | rM3D (Gs) | Syn | IRES-mCitrine | Yes | Roth | |
| 50460 | pAAV-EF1a-DIO-hM3D(Gq)-mCherry | hM3D (Gq) | EF1a | mCherry | Yes | Roth | |
| 50461 | pAAV-EF1a-DIO-hM4D(Gi)-mCherry | hM4D (Gi) | EF1a | mCherry | Yes | Roth | |
| 50463 | pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine | hM3D (Gq) | Syn | IRES-mCitrine | No | Roth | |
| 50464 | pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine | hM4D (Gi) | Syn | IRES-mCitrine | No | Roth | |
| 50466 | pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine |
hM3D (Gq) | CaMKIIa | IRES-mCitrine | No | Roth | |
| 50467 | pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine |
hM4D (Gi) | CaMKIIa | IRES-mCitrine | No | Roth | |
| 50468 | pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine | rM3D (Gs) | CaMKIIa | IRES-mCitrine | No | Roth | |
| 50470 | pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine | hM3D (Gq) | GFAP | IRES-mCitrine | No | Roth | |
| 50471 | pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine | hM4D (Gi) | GFAP | IRES-mCitrine | No | Roth | |
| 50472 | pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine | rM3D (Gs) | GFAP | IRES-mCitrine | No | Roth | |
| 45547 | pcDNA5/FRT-HA-hM3D(Gq) | hM3D (Gq) | CMV | No | Roth | ||
| 45548 | pcDNA5/FRT-HA-hM4D(Gi) | hM4D (Gi) | CMV | No | Roth | ||
| 45549 | pcDNA5/FRT-HA-rM3D(Gs) | rM3D (Gs) | CMV | No | Roth | ||
| 70717 | AAV-mOXT-hM3Dq-mCherry-WPRE | hM3D (Gq) | Mouse Oxycotin | mCherry | No | Geschwind | |
| 66795 | pAAV-PTRE-tight-hM3Dq-mCherry | hM3D (Gq) | Tet-inducible | mCherry | No | Wisden | |
| 81008 | pAAV-CBA-DIO-hM4Di-mCherry | hM4D (Gi) | CBA | mCherry | Yes | Sabatini | |
| 135635 | pAAV-S5E2-Gq-P2A-dTomato | hM3D (Gq) | E2 Enhancer | tdTomato | No | Dimidschstein | |
| 111397 | pAAV-hSyn-DIO {hCAR}off-{hM4Di-mCherry}on-W3SL | hM4D (Gi) | Syn | mCherry | Yes | Kepecs | |
| 92285 | pZac2.1 GfaABC1D rM3D mCherry SV40 | rM3D (Gs) | GfaABC1D | mCherry | No | Khakh | |
| 92284 | pZac2.1 GfaABC1D hM3D mCherry SV40 | hM3D (Gq) | GfaABC1D | mCherry | No | Khakh | |
| 92286 | pZac2.1 GfaABC1D hM4D mCherry SV40 | hM4D (Gi) | GfaABC1D | mCherry | No | Khakh | |
| 125146 | pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi) | hM4D (Gi) | Syn | Yes | Richie | ||
| 125147 | pOTTC1761 - pAAV SYN1 DIO HA-hM3D(Gq) | hM3D (Gq) | Syn | Yes | Richie | ||
| 121538 | pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) |
hM4D (Gi) | Syn | No | Richie | ||
| 121539 | pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) |
hM3D (Gq) | Syn | No | Richie | ||
| 50478 | pAAV-GFAP-hM3D(Gq)-mCherry |
hM3D (Gq) | GFAP | mCherry | No | Roth | |
| 83896 | pAAV-hDlx-GiDREADD-dTomato-Fishell-5 |
hM4D (Gi) | hDlx | tdTomato | No | Fishell | |
| 83897 | pAAV-hDlx-GqDREADD-dTomato-Fishell-4 |
hM3D (Gq) | hDlx | tdTomato | No | Fishell | |
| 154867 | pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA |
hM4D (Gi) | Syn | mCherry | Flp-dependent | Gether | |
| 154869 | pAAV-hSyn-fDIO-rM3D(Gs)-mCherry-WPREpA | rM3D (Gs) | Syn | mCherry | Flp-dependent | Gether | |
| 154868 | pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA |
hM3D (Gq) | Syn | mCherry | Flp-dependent | Gether | |
| 89150 | pOTTC1329 - pAAV EF1a HA-hM4D(Gi) | hM4D (Gi) | EF1a | No | Harvey | ||
| 89149 | pOTTC1328 - pAAV EF1a HA-hM3D(Gq) | hM3D (Gq) | EF1a | No | Harvey | ||
| 60544 | AA0250-mCherry-2a-hM4D-nrxn1a rev | hM4Dnrxn (Gi) | CAG | mCherry | Yes | Sternson | |
| 52525 | CMV:: HA-hM4Dnrxn | hM4Dnrxn (Gi) | CMV | No | Sternson | ||
| 52523 | CAG:: mCherry-2a-hM4Dnrxn | hM4Dnrxn (Gi) | CAG | mCherry | No | Sternson | |
| 52521 | AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D | hM4Dnrxn (Gi) | CAG | ChR2-2a | Yes | Sternson | |
| 52520 | CAG:: ChR2HA-2a-hM4D | hM4Dnrxn (Gi) | CAG | ChR2-2a | No | Sternson | |
| 159457 | AAVS1-pur-CAG-Bi-DREADD | hM3D(Gq), KORD | CAG | mCherry | No | Chen | |
| 32483 | rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR | PSAML141F,Y115F-GlyR | CAG | ChR2 2A | Yes | Sterson | |
| 32482 | CAG::PSAMQ79G:GlyR-IRES-GFP | PSAMQ79G-GlyR | CAG | IRES-EGFP | No | Sterson | |
| 32481 | rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP | PSAML141F,Y115F-GlyR | Syn | IRES-EGFP | Yes | Sterson | |
| 32480 | CAG::PSAML141F,Y115F:GlyR-IRES-GFP | PSAML141F,Y115F-GlyR | CAG | IRES-EGFP | No | Sterson | |
| 32479 | rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP | PSAML141F-GlyR | Syn | IRES-EGFP | Yes | Sterson | |
| 32478 | CAG::PSAML141F:GlyR-IRES-GFP | PSAML141F-GlyR | CAG | IRES-EGFP | Yes | Sterson | |
| 32477 | rAAV-syn::FLEX-rev::PSAML141F,Y115F:5HT3HC-IRES-GFP | PSAML141F,Y115F-5HT3HC | Syn | IRES-EGFP | Yes | Sterson | |
| 32476 | CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP | PSAML141F,Y115F-5HT3HC | CAG | IRES-EGFP | No | Sterson | |
| 32475 | rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP | PSAMQ79G,Q139G:5HT3HC | Syn | IRES-EGFP | Yes | Sterson | |
| 32474 | CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP | PSAMQ79G,Q139G:5HT3HC | CAG | IRES-EGFP | No | Sterson | |
| 119739 | pCAG PSAM4 GlyR IRES EGFP | PSAM4-GlyR | CAG | IRES-EGFP | No | Sterson | |
| 119740 | pCAG PSAM4-5HT3 HC IRES EGFP | PSAM4-5HT3 HC | CAG | IRES-EGFP | No | Sterson | |
| 119741 | AAV SYN flex PSAM4 GlyR IRES EGFP |
PSAM4-GlyR | Syn | IRES-EGFP | Yes | Sterson | |
| 119742 | AAV SYN PSAM4 GlyR IRES EGFP |
PSAM4-GlyR | Syn | IRES-EGFP | No | Sterson | |
| 119744 | AAV CAMKII PSAM4 GlyR IRES EGFP |
PSAM4-GlyR | CamKII | IRES-EGFP | No | Sterson | |
| 123066 | pCAG PSAM4 5HT3 LC IRES GFP | PSAM4-5HT3 LC | CAG | IRES-EGFP | No | Sterson |
Currently, no available plasmids.
Browse all DREADD plasmids
Do you have suggestions for other plasmids that should be added to this list?
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