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PurposeGq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 50463 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 7686
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehM3D(Gq)-IRES-mCitrine
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SpeciesH. sapiens (human)
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Insert Size (bp)3124
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MutationSee supplemental documents for DREADD mutations
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Entrez GeneCHRM3 (a.k.a. EGBRS, HM3, PBS)
- Promoter human Synapsin 1
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal I (not destroyed)
- 3′ cloning site EcoR I (not destroyed)
- 5′ sequencing primer TCGTGTCGTGCCTGAGAGCG
- 3′ sequencing primer GCATTAAAGCAGCGTATCCACATAGC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine was a gift from Bryan Roth (Addgene plasmid # 50463 ; http://n2t.net/addgene:50463 ; RRID:Addgene_50463)