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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32476 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGS- IRES-GFP
- Backbone size w/o insert (bp) 7200
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Modifications to backboneWPRE (Woodchuck hepatitis virus posttranscriptional regulatory element) before polyA sequence
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePSAM Y115F,L141F:5HT3 HC
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SpeciesH. sapiens (human), M. musculus (mouse)
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Insert Size (bp)1415
- Promoter CAG
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Tag
/ Fusion Protein
- IRES-GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CTGCTAACCATGTTCATGC
- 3′ sequencing primer CAGAAACGCAAGAGTCTTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP was a gift from Scott Sternson (Addgene plasmid # 32476 ; http://n2t.net/addgene:32476 ; RRID:Addgene_32476) -
For your References section:
Chemical and genetic engineering of selective ion channel-ligand interactions. Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM. Science. 2011 Sep 2;333(6047):1292-6. 10.1126/science.1206606 PubMed 21885782