PurposeExpresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66795||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 7159
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert namepTIGHT promoter-hM3Dq-mCherry
Insert Size (bp)4400
- Promoter pTIGHT TRE
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (unknown if destroyed)
- 3′ cloning site NheI (unknown if destroyed)
- 5′ sequencing primer Tggaaagagaactgtgcctc
- 3′ sequencing primer Cagttcatgtacggctccaag (Common Sequencing Primers)
hM3Dq-mCherry reading frame was obtained from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene plasmid #44361) and cloned in the sense orientation between the lox sites. Please see the associated publication for additional cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-PTRE-tight-hM3Dq-mCherry was a gift from William Wisden (Addgene plasmid # 66795 ; http://n2t.net/addgene:66795 ; RRID:Addgene_66795)
For your References section:Neuronal ensembles sufficient for recovery sleep and the sedative actions of alpha2 adrenergic agonists. Zhang Z, Ferretti V, Guntan I, Moro A, Steinberg EA, Ye Z, Zecharia AY, Yu X, Vyssotski AL, Brickley SG, Yustos R, Pillidge ZE, Harding EC, Wisden W, Franks NP. Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015 Feb 23. 10.1038/nn.3957 PubMed 25706476