pAAV-PTRE-tight-hM3Dq-mCherry
(Plasmid #66795)

• Purpose: Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging
• Depositing Lab: William Wisden
• Publication: Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015 Feb 23.

Backbone

• Vector backbone: pUC
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 7159
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pTIGHT promoter-hM3Dq-mCherry
• Species: Synthetic
• Insert Size (bp): 4400
• Promoter: pTIGHT TRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: Tggaaagagaactgtgcctc
• 3′ sequencing primer: Cagttcatgtacggctccaag

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Brian Roth provided the hM3Dq-mCherry gene
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

hM3Dq-mCherry reading frame was obtained from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene plasmid #44361) and cloned in the sense orientation between the lox sites. Please see the associated publication for additional cloning details.