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pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)
(Plasmid #125146)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 125146 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pOTTC901 - pAAV SYN1 DIO GIRK4-Myc
  • Backbone manufacturer
    NIDA_GEVVC
  • Backbone size w/o insert (bp) 4811
  • Total vector size (bp) 6124
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HA-tagged hM4D(Gi)
  • Alt name
    Inhibitory DREADD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1470
  • GenBank ID
    1132 CHRM4
  • Entrez Gene
    CHRM4 (a.k.a. HM4, M4R)
  • Promoter SYN1
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer hSYN1 F417 ACTCAGCGCTGCCTCAGTCT
  • 3′ sequencing primer WPRE R1 ATGAAAGCCATACGGGAAGC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi) was a gift from Christopher Richie (Addgene plasmid # 125146 ; http://n2t.net/addgene:125146 ; RRID:Addgene_125146)