pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine
(Plasmid #50471)

• Purpose: Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter
• Depositing Lab: Bryan Roth
• Publication: Roth lab DREADDs (unpublished)

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 7558
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hM4D(Gi)-IRES-mCitrine
• Species: H. sapiens (human)
• Insert Size (bp): 2792
• Mutation: See supplemental documents for DREADD mutations
• Entrez Gene: CHRM4 (a.k.a. HM4, M4R)
• Promoter: GFAP
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: CCGGGCATCGCCAGTCTAGC
• 3′ sequencing primer: GCATTAAAGCAGCGTATCCACATAGC

Resource Information

• Supplemental Documents:
  • DREADD mutations
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry