pAAV-GFAP-EGFP
(Plasmid #50473)

• Purpose: EGFP under the control of GFAP promoter
• Depositing Lab: Bryan Roth
• Publication: Roth lab DREADDs (unpublished)

Backbone

• Vector backbone: pAAV
  (Search Vector Database)
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 5490
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EGFP
• Species: Aequorea victoria
• Insert Size (bp): 726
• Promoter: GFAP
• Tag / Fusion Protein:
  • N/A

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal I (not destroyed)
• 3′ cloning site: EcoR I (not destroyed)
• 5′ sequencing primer: CCGGGCATCGCCAGTCTAGC
• 3′ sequencing primer: GCATTAAAGCAGCGTATCCACATAGC

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry