pOTTC1329 - pAAV EF1a HA-hM4D(Gi)
PurposeAn AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||89150||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepAAV EF1a DIO
Backbone manufacturerKarl Deisseroth
- Backbone size w/o insert (bp) 5239
Modifications to backbonelox sites were removed when CDS was added between BamHI and EcoRV
Vector typeMammalian Expression, AAV
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberLow Copy
Gene/Insert nameHA-tagged hM4D(Gi)
Alt nameGq-coupled inhibitory DREADD
- Promoter EF1a
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer EF1a F1
- 3′ sequencing primer WPRE R1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOTTC1329 - pAAV EF1a HA-hM4D(Gi) was a gift from Brandon Harvey (Addgene plasmid # 89150 ; http://n2t.net/addgene:89150 ; RRID:Addgene_89150)
For your References section:Chemogenetics revealed: DREADD occupancy and activation via converted clozapine. Gomez JL, Bonaventura J, Lesniak W, Mathews WB, Sysa-Shah P, Rodriguez LA, Ellis RJ, Richie CT, Harvey BK, Dannals RF, Pomper MG, Bonci A, Michaelides M. Science. 2017 Aug 4;357(6350):503-507. doi: 10.1126/science.aan2475. 10.1126/science.aan2475 PubMed 28774929