pAAV-6xTRE-FLEX-hM4Di.mCherry
(Plasmid
#169415)
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PurposeActivity-dependent, cre-dependent, temporally restricted inhibitory DREADD expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehM4Di.mCherry
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SpeciesSynthetic
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MutationhM4Di is Gi-coupled DREADD.Derived from human muscarinic receptor M4 sequence with two point mutations (Y113C,A203G)
- Promoter Minimal TATA
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Modified Tet operator repeats. Construct might show tTA-independent "leaky" expression in some cell types.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-6xTRE-FLEX-hM4Di.mCherry was a gift from David Talmage (Addgene plasmid # 169415 ; http://n2t.net/addgene:169415 ; RRID:Addgene_169415) -
For your References section:
Functionally refined encoding of threat memory by distinct populations of basal forebrain cholinergic projection neurons. Rajebhosale P, Ananth MR, Kim R, Crouse RB, Jiang L, Lopez-Hernandez G, Zhong C, Arty C, Wang S, Jone A, Desai NS, Li Y, Picciotto MR, Role LW, Talmage DA. Elife. 2024 Feb 16;13:e86581. doi: 10.7554/eLife.86581. 10.7554/eLife.86581 PubMed 38363713
