Phagemid Synuclein VHH Immune Library
(Pooled Library #1000000071)
Synuclein phagemid VHH phage display library. The depositors routinely use VCSM13 as helper phage, but other helper phage should be fine.
- Vector Backbone
- Depositing Labs
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||1000000071||Phage display library||1||$300||Add to Cart|
For further manipulation, please use the growth strain/temperature recommended in your protocol as these will vary by method.
This phage display library will be shipped frozen on dry ice.
Volume∼100µL aliquot of frozen bacterial glycerol stock (TG1 E. coli )
Terms and Licenses
This phage display library will be provided as an aliquot of frozen bacterial glycerol stock (TG1 E. coli ) and will not be sequenced. For further manipulation, please use the growth stain/temperature recommended in your protocol as these will vary by method.
Library screening can be done by many approaches and may need to be tailored to the different targets and the reagents available. The depositor does not recommend amplification unless the primary library becomes unavailable for use in panning as amplification is known to create bias and reduce complexity. If amplification is required, or for general procedures, the depositor recommends following standard procedures such as Barbas CF. Phage display: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 2001.
For general information, the depositor also recommends the following references:
A novel strategy for development of recombinant antitoxin therapeutics tested in a mouse botulism model. Mukherjee J, Tremblay JM, Leysath CE, Ofori K, Baldwin K, Feng X, Bedenice D, Webb RP, Wright PM, Smith LA, Tzipori S, Shoemaker CB. PLoS One. 2012;7(1):e29941. doi: 10.1371/journal.pone.0029941. PubMed PMID: 22238680 .
Alpaca (Lama pacos) as a convenient source of recombinant camelid heavy chain antibodies (VHHs). Maass DR, Sepulveda J, Pernthaner A, Shoemaker CB. J Immunol Methods. 2007 Jul 31;324(1-2):13-25. PubMed PMID: 17568607 .
For more information about this library project, refer to the MJFF or MJFF Grant Funding web pages.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Synuclein VHH Immune Library was a gift from Anne Messer and MJFF (Addgene #1000000071)
For your References section:Computational affinity maturation of camelid single-domain intrabodies against the nonamyloid component of alpha-synuclein. Mahajan SP, Meksiriporn B, Waraho-Zhmayev D, Weyant KB, Kocer I, Butler DC, Messer A, Escobedo FA, DeLisa MP. Sci Rep. 2018 Dec 4;8(1):17611. doi: 10.1038/s41598-018-35464-7. PubMed 30514850