This phage display library will be provided as an aliquot of frozen bacterial glycerol stock (TG1 E. coli ) and will not be sequenced. For further manipulation, please use the growth stain/temperature recommended in your protocol as these will vary by method.
Library screening can be done by many approaches and may need to be tailored to the different targets and the reagents available. The depositor does not recommend amplification unless the primary library becomes unavailable for use in panning as amplification is known to create bias and reduce complexity. If amplification is required, or for general procedures, the depositor recommends following standard procedures such as
Barbas CF. Phage display: a laboratory manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 2001.
For general information, the depositor also recommends the following references:
A novel strategy for development of recombinant antitoxin therapeutics tested in a mouse botulism model. Mukherjee J, Tremblay JM, Leysath CE, Ofori K, Baldwin K, Feng X, Bedenice D, Webb RP, Wright PM, Smith LA, Tzipori S, Shoemaker CB. PLoS One. 2012;7(1):e29941. doi: 10.1371/journal.pone.0029941. PubMed
Alpaca (Lama pacos) as a convenient source of recombinant camelid heavy chain antibodies (VHHs). Maass DR, Sepulveda J, Pernthaner A, Shoemaker CB. J Immunol Methods. 2007 Jul 31;324(1-2):13-25. PubMed
For more information about this library project, refer to the
MJFF Grant Funding