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MXS Chaining Kit
(Kit # 1000000064 )

Depositing Lab:   Pierre Neveu

The MXS-chaining system allows efficient assembly of constructs that are optimized for synthetic biology applications in mammalian systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building blocks: 14 different fluorescent proteins, 10 mammalian promoters and enhancers, 3 polyA signals as well as selection cassettes and tools for inducible gene expression.

This kit contains 55 plasmids and is shipped as bacterial glycerol stocks in a 96-well plate format.

Add to Cart
$375 USD + shipping

Available to academics and nonprofits only.

Original Publication

MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. Sladitschek HL, Neveu PA. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PubMed.

Description

The MXS-Chaining Kit is a cloning platform that utilizes an iterative chaining process to combine individual building blocks, either preconstructed or custom-designed MXS plasmids, in a desired order to generate new plasmids optimized for fluorescence imaging or flow cytometry applications in mammalian cells. The MluI, XhoI and SalI (MXS) enzymes permit fusion of tags or fluorescent proteins to a coding sequence of interest, as the ligation scar is translated to valine-glutamic acid. Similar to other chaining based methods, the original pattern of restriction sites is regenerated after ligation of the two building blocks and the chaining direction is determined by the choice of enzymes used for the ligation. This cloning method has been used to modularly assemble highly similar sequences for various experimental procedures, including subcellular localization or visualization of processes in single, live cells.

Overview of MXS Chaining System

Overview of MXS Chaining procedure

Figure 1. Schematic overview of the MXS-chaining method. Standardized building blocks can be chained directionally using the restriction enzymes MluI, XhoI and SalI.

Kit Documentation

Documents included with shipment:

Icon MXS Chaining Kit info (75.7 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The MXS-Chaining kit was a gift from Pierre Neveu (Addgene kit # 1000000064).”

For your Reference section:

MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. Sladitschek HL, Neveu PA. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PubMed .

MXS Chaining Kit - #1000000064

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
A / 1 MXS_chaining vector
Ampicillin
A / 2 MXS_MCS1
Ampicillin
A / 3 MXS_MCS2
Ampicillin
A / 4 MXS_MCS3
Ampicillin
A / 5 MXS_MCS4
Ampicillin
A / 6 SMX DEST vector
Ampicillin
A / 7 MXS_TagBFP
Ampicillin
A / 8 MXS_Cerulean
Ampicillin
A / 9 MXS_LSS-mKate2
Ampicillin
A / 10 MXS_mEGFP
Ampicillin
A / 11 MXS_mAzamiGreen
Ampicillin
A / 12 MXS_Citrine
Ampicillin
B / 1 MXS_mKO2
Ampicillin
B / 2 MXS_tdTomato
Ampicillin
B / 3 MXS_mCherry
Ampicillin
B / 4 MXS_mPlum
Ampicillin
B / 5 MXS_E2-Crimson
Ampicillin
B / 6 MXS_iRFP670
Ampicillin
B / 7 MXS_PA-GFP
Ampicillin
B / 8 MXS_Kaede
Ampicillin
B / 9 MXS_PGK
Ampicillin
B / 10 MXS_minPGK
Ampicillin
B / 11 MXS_PGKenhancer
Ampicillin
B / 12 MXS_CMV
Ampicillin
C / 1 MXS_minCMV
Ampicillin
C / 2 MXS_CMVenhancer
Ampicillin
C / 3 MXS_CAG
Ampicillin
C / 4 MXS_EF1a
Ampicillin
C / 5 MXS_Tet
Ampicillin
C / 6 MXS_BiTet
Ampicillin
C / 7 MXS_bGpA
Ampicillin
C / 8 MXS_bGHpA
Ampicillin
C / 9 MXS_SV40pA
Ampicillin
C / 10 MXS_CreERT2
Ampicillin
C / 11 MXS_rtTA3
Ampicillin
C / 12 MXS_tTA2
Ampicillin
D / 1 MXS_loxP
Ampicillin
D / 2 MXS_linker
Ampicillin
D / 3 MXS_H2B
Ampicillin
D / 4 MXS_P2A
Ampicillin
D / 5 MXS_PEST2D
Ampicillin
D / 6 MXS_CMV::HygroR-bGHpA
Ampicillin
D / 7 MXS_PGK::HygroR-bGHpA
Ampicillin
D / 8 MXS_CMV::NeoR-bGHpA
Ampicillin
D / 9 MXS_PGK::NeoR-bGHpA
Ampicillin
D / 10 MXS_CMV::PuroR-bGHpA
Ampicillin
D / 11 MXS_PGK::PuroR-bGHpA
Ampicillin
D / 12 MXS_CMV::ZeoR-bGHpA
Ampicillin
E / 1 MXS_PGK::ZeoR-bGHpA
Ampicillin
E / 2 MXS_CMV::CreERT2-bGHpA
Ampicillin
E / 3 MXS_PGK::CreERT2-bGHpA
Ampicillin
E / 4 MXS_CMV::rtTA3-bGHpA
Ampicillin
E / 5 MXS_PGK::rtTA3-bGHpA
Ampicillin
E / 6 MXS_CMV::tTA2-bGHpA
Ampicillin
E / 7 MXS_PGK::tTA2-bGHpA
Ampicillin
Data calculated @ 2017-03-25

Kit Plate Map - #1000000064

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