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Rapid neurogenesis through transcriptional activation in human stem cells.
Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM
Mol Syst Biol. 2014 Nov 17;10(11):760. doi: 10.15252/msb.20145508.
PubMed

Plasmids from Article

ID Plasmid Purpose  
61471pLV_TRET_Ngn2‐2A‐Ngn13rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1
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61472pLV_hEF1a_rtTA33rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)
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61473pLV_TRET_hNgn1_UBC_Bla3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker
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61474pLV_TRET_hNgn2_UBC_Puro3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker
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