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Efficient generation of human iPSCs by a synthetic self-replicative RNA.
Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF
Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
58970T7-VEE-IRES-Purononinfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
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58971SP6-VEE-IRES-Purononinfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
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58972T7-VEE-OKS-iMRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
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58973SP6-VEE-OKS-iMRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
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58974T7-VEE-OKS-iGRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
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58975SP6-VEE-OKS-iGRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
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58976SP6-VEE-GFPexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
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58977T7-VEE-GFPexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
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58978pTNT-B18Rexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs
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58979pTNT-B18R-6Hisexpresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs
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Recombinant Antibodies from Article