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Purposeexpression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone
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Vector backboneSp6-VEE-IRES-puro (Addgene plasmid 58971)
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Backbone manufacturerDowdy lab
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Vector typeMammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesAequorea victoria
- Promoter 26S subgenomic promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG) (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For sequence and maps, please see T7-VEE-GFP (Addgene plasmid 58977).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SP6-VEE-GFP was a gift from Steven Dowdy (Addgene plasmid # 58976 ; http://n2t.net/addgene:58976 ; RRID:Addgene_58976) -
For your References section:
Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086